Q59182 (TPIS_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Synonyms:	tpi
Ordered Locus Names:	BB_0055

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Triosephosphate isomerase HAMAP MF_00147_B		PRO_0000090184
Sites
	Active site	96	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity
Experimental info
	Sequence conflict	201	1	S → T in AAB53932. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59182 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 8EDE97F4EF616495	FASTA	253	27,759
	10 20 30 40 50 60 MRKTFLAGNW KMHYTSAEAS IVAKKIATEV KTLKDDVVIM ITPPFTALSK VSECIKGSNI 70 80 90 100 110 120 LLGAQNMSYM ESGARTSEIS PSMLLEFGVE YVILGHSECR LYLAETDEII NKKILAGLKH 130 140 150 160 170 180 PFKYLILCVG ETLDERDSGK TLEVVLNQVK KGLNCVSESD IQRIILAYEP VWAIGTGKTA 190 200 210 220 230 240 TKEEAEEVHK AIRLEITKLY SKSASDNIII QYGGSVNSNN VKELMNEPNI DGALIGGASL 250 KAESFLSIIN NVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A glyceraldehyde-3-phosphate dehydrogenase homolog in Borrelia burgdorferi and Borrelia hermsii." Anda P., Gebbia J.A., Backenson P.B., Coleman J.L., Benach J.L. Infect. Immun. 64:262-268(1996) [PubMed: 8557349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[3]	"Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzelii." Ojaimi C., Davidson B.E., Saint-Girons I., Old I.G. Microbiology 140:2931-2940(1994) [PubMed: 7812434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74. Strain: 212.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28760 Genomic DNA. Translation: AAB53932.1. AE000783 Genomic DNA. Translation: AAC66452.1. L32595 Genomic DNA. Translation: AAC41406.1.
PIR	G70106.
RefSeq	NP_212189.1. NC_001318.1.
3D structure databases
ProteinModelPortal	Q59182.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000009137; EBBORP00000008467; EBBORG00000009136.
GeneID	1194891.
GenomeReviews	Gene locus BB_0055 in contig AE000783_GR.
KEGG	bbu:BB0055.
NMPDR	fig|224326.1.peg.439.
PATRIC	20556715. VBIBorBur75917_0453.
TIGR	BB_0055.
Phylogenomic databases
GeneTree	EBGT00050000007284.
HOGENOM	HBG708281.
OMA	VCVGETQ.
PhylomeDB	Q59182.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0055-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BORBU

Accession

Primary (citable) accession number: Q59182
Secondary accession number(s): Q59187

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program