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Q59181 (PGK_BORBU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:BB_0056
OrganismBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) [Reference proteome] [HAMAP]
Taxonomic identifier224326 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145911

Regions

Nucleotide binding350 – 3534ATP By similarity
Region22 – 243Substrate binding By similarity
Region60 – 634Substrate binding By similarity

Sites

Binding site371Substrate By similarity
Binding site1191Substrate By similarity
Binding site1521Substrate By similarity
Binding site2021ATP By similarity
Binding site2931ATP; via carbonyl oxygen By similarity
Binding site3241ATP By similarity

Experimental info

Sequence conflict1641L → S in AAB53931. Ref.1
Sequence conflict177 – 1782KF → NS in AAB53931. Ref.1
Sequence conflict1901F → S in AAB53931. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q59181 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: EB437EFD5F7AFFDE

FASTA39342,346
        10         20         30         40         50         60 
MSIKTVKDFS SFAGKRALVR CDFNVPLKEG SISDDTRIRA ALSTIEYLKE RGARIVLVSH 

        70         80         90        100        110        120 
LGRPDGKKNP KYSLKPVANR LSELLGQDVK MLSDCIGSEV VNSTLQMKDG DVVLLENVRF 

       130        140        150        160        170        180 
YAEEEKNDKN FAKKLSENGD VFVNDAFGAA HRAHASTVGV ADYLPSVGGF LMEKEDKFLG 

       190        200        210        220        230        240 
GILKNPERPF VSIIGGSKVS SKIAVLESLL SKSNVVVIGG GMAYTFLHSE GYSIGKSLLE 

       250        260        270        280        290        300 
DEYIGIASSF LKKAKELGVK VILPLDHIVA DDFNKNSIPE YIDSFNIPEN KIGMDIGANT 

       310        320        330        340        350        360 
LKEIENVVKT AKTIIWNGPL GVFEFDSFSK GTAKVAEMVA SCSGLTVVGG GDSVAAVNKF 

       370        380        390 
NLSDKITHVS TGGGASLEYL EGRILPGIKV LEN 

« Hide

References

« Hide 'large scale' references
[1]"A glyceraldehyde-3-phosphate dehydrogenase homolog in Borrelia burgdorferi and Borrelia hermsii."
Anda P., Gebbia J.A., Backenson P.B., Coleman J.L., Benach J.L.
Infect. Immun. 64:262-268(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi."
Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. expand/collapse author list , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C.
Nature 390:580-586(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[3]"Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzelii."
Ojaimi C., Davidson B.E., Saint-Girons I., Old I.G.
Microbiology 140:2931-2940(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 346-393.
Strain: 212.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28760 Genomic DNA. Translation: AAB53931.1.
AE000783 Genomic DNA. Translation: AAC66451.1.
L32595 Genomic DNA. Translation: AAC41405.1.
PIRH70106.
RefSeqNP_212190.1. NC_001318.1.

3D structure databases

ProteinModelPortalQ59181.
SMRQ59181. Positions 1-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224326.BB0056.

Proteomic databases

PRIDEQ59181.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC66451; AAC66451; BB_0056.
GeneID1194892.
KEGGbbu:BB_0056.
PATRIC20556717. VBIBorBur75917_0454.

Phylogenomic databases

eggNOGCOG0126.
KOK00927.
OMAAGHPVGK.
OrthoDBEOG64N9Z0.

Enzyme and pathway databases

BioCycBBUR224326:G9SO-55-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
ProtoNetSearch...

Entry information

Entry namePGK_BORBU
AccessionPrimary (citable) accession number: Q59181
Secondary accession number(s): Q59186
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways