Q59179 (TPIS_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Synonyms:	tpi
Ordered Locus Names:	BUsg_297

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP-Rule MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP-Rule MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Triosephosphate isomerase HAMAP-Rule MF_00147		PRO_0000090196
Sites
	Active site	96	1	Electrophile By similarity
	Active site	168	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59179 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: DFA12B60F9E68E10	FASTA	254	28,458
	10 20 30 40 50 60 MKKKIIAANW KLNGSIKTIS YFLTFLKSQI SSFLKNNIII IAPPTVFLER VYKDINSINI 70 80 90 100 110 120 HLAAQNIDVN LTGAFTGENS ALMMKDIGVK YIIIGHSERR LLHNENNEII AKKFCLVKNL 130 140 150 160 170 180 NLVPILCIGE TEAEKKSNKT EKILKEQLNS IFNSFGEKAF RNAVIAYEPI WSIGTGVSAD 190 200 210 220 230 240 PKNVQLIHKF IKNYIKKYDI VSAENLIVQY GGSVTSLNAG NFLKQPDIDG LLIGSASLKH 250 EEFLKIIKIS DSFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA." Thao M.L., Baumann P. Curr. Microbiol. 35:68-69(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43549 Genomic DNA. Translation: AAC05431.1. AE013218 Genomic DNA. Translation: AAM67852.1.
RefSeq	NP_660641.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q59179.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg297.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67852; AAM67852; BUsg_297.
GeneID	1005501.
KEGG	bas:BUsg297.
PATRIC	21247403. VBIBucAph100086_0311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
KO	K01803.
OMA	QEVCGAI.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-354-MONOMER.
UniPathway	UPA00109; UER00189. UPA00138.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00147_B. TIM_B.
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
PANTHER	PTHR21139. PTHR21139. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BUCAP

Accession

Primary (citable) accession number: Q59179

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program