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Q59179 (TPIS_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Triosephosphate isomerase

Short name=TIM
EC=5.3.1.1
Alternative name(s):
Triose-phosphate isomerase
Gene names
Name:tpiA
Synonyms:tpi
Ordered Locus Names:BUsg_297
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP-Rule MF_00147

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00147

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP-Rule MF_00147

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00147

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00147.

Sequence similarities

Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
Pentose shunt
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontriose-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Triosephosphate isomerase HAMAP-Rule MF_00147
PRO_0000090196

Sites

Active site961Electrophile By similarity
Active site1681Proton acceptor By similarity
Binding site91Substrate By similarity
Binding site111Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59179 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DFA12B60F9E68E10

FASTA25428,458
        10         20         30         40         50         60 
MKKKIIAANW KLNGSIKTIS YFLTFLKSQI SSFLKNNIII IAPPTVFLER VYKDINSINI 

        70         80         90        100        110        120 
HLAAQNIDVN LTGAFTGENS ALMMKDIGVK YIIIGHSERR LLHNENNEII AKKFCLVKNL 

       130        140        150        160        170        180 
NLVPILCIGE TEAEKKSNKT EKILKEQLNS IFNSFGEKAF RNAVIAYEPI WSIGTGVSAD 

       190        200        210        220        230        240 
PKNVQLIHKF IKNYIKKYDI VSAENLIVQY GGSVTSLNAG NFLKQPDIDG LLIGSASLKH 

       250 
EEFLKIIKIS DSFL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes aspS-trxB-serS-serC-aroA-rpsA-himD-tpiA."
Thao M.L., Baumann P.
Curr. Microbiol. 35:68-69(1997)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43549 Genomic DNA. Translation: AAC05431.1.
AE013218 Genomic DNA. Translation: AAM67852.1.
RefSeqNP_660641.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ59179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg297.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67852; AAM67852; BUsg_297.
GeneID1005501.
KEGGbas:BUsg297.
PATRIC21247403. VBIBucAph100086_0311.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0149.
KOK01803.
OMAAKLGCTF.
OrthoDBEOG66QM23.
ProtClustDBPRK00042.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-311-MONOMER.
UniPathwayUPA00109; UER00189.
UPA00138.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00147_B. TIM_B.
InterProIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERPTHR21139. PTHR21139. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMSSF51351. SSF51351. 1 hit.
TIGRFAMsTIGR00419. tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPIS_BUCAP
AccessionPrimary (citable) accession number: Q59179
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names