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Q59167 (ACSA2_GLUHA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cellulose synthase 2

Including the following 2 domains:

  1. Cellulose synthase catalytic subunit [UDP-forming]
    EC=2.4.1.12
  2. Cyclic di-GMP-binding domain
    Alternative name(s):
    Cellulose synthase 2 regulatory domain
Gene names
Name:acsAII
OrganismGluconacetobacter hansenii (Acetobacter hansenii)
Taxonomic identifier436 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter ›

Protein attributes

Sequence length1596 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Subcellular location

Cell inner membrane; Multi-pass membrane protein Potential.

Domain

There are two conserved domains in the globular part of the catalytic subunit: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.

Miscellaneous

It is not essential for cellulose production in this strain.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 2 family.

In the C-terminal section; belongs to the AcsB/BcsB family.

Contains 1 PilZ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15961596Cellulose synthase 2
PRO_0000059261

Regions

Transmembrane25 – 4521Helical; Potential
Transmembrane106 – 12621Helical; Potential
Transmembrane396 – 41621Helical; Potential
Transmembrane421 – 44121Helical; Potential
Transmembrane505 – 52521Helical; Potential
Transmembrane544 – 56421Helical; Potential
Transmembrane1553 – 157321Helical; Potential
Domain570 – 669100PilZ
Region1 – 749749Catalytic
Region145 – 23894Catalytic subdomain A
Region315 – 37561Catalytic subdomain B
Region750 – 1596847Cyclic di-GMP binding Potential

Sites

Active site1871 Potential
Active site3311 Potential
Binding site2341Substrate Potential
Binding site2361Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q59167 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D98A6F6259E1F3CE

FASTA1,596175,801
        10         20         30         40         50         60 
MIYRAILKRL RLEQLARVPA VSAASPFVMM AVGVFLMLMA GGVTISTTSQ AFVTCGTVGL 

        70         80         90        100        110        120 
FLLLKGRKGR GVTCFLMMLS LLVSLRYMVW RLTTTLELHS PLQAALSLLL VAAELYALLT 

       130        140        150        160        170        180 
LCLSYFQMSW PLDRKPLPLP ADTTDWPVVD VYVPSYNEEL SLVRSTVLGA LAIDWPADKL 

       190        200        210        220        230        240 
NVYILDDGRR KSFHAFAMEA GAGYIIRDQN NHAKAGNLNH ALRVTEGEYV VIFDCDHIPT 

       250        260        270        280        290        300 
RGFLKKTIGW MMADPKLALL QTPHHFYSPD PFQRNLATGQ NVPPEGNMFY GLVQDGNDFW 

       310        320        330        340        350        360 
DATFFCGSCA AIRRSAVLGI GGFATETVTE DAHTALKMQR EGWHTAYLRQ PLAAGLSTER 

       370        380        390        400        410        420 
LMLHIGQRVR WARGMLQIMR LDNPLLGSGL RWQQRLCYLS AMSHFLFAIP RLVFLASPLA 

       430        440        450        460        470        480 
FLFLGQNIIA ASPFAILVYA FPHVFHSIGT LSRVEGRWRY SFWSEIYETT LALFLVRVTI 

       490        500        510        520        530        540 
MTLLNPRKGE FNVTDKGGLL QSEYFDLNAV YPNVILAVIL ALALVRGIGG MMWEYHDRLA 

       550        560        570        580        590        600 
LQSFALNTLW VAVSLIIVLA SIAVGRETRQ IRHKPRVRAT LPITLIDEHG QHYHAHTSDI 

       610        620        630        640        650        660 
SLGGIAARLS TEHALPTQTR VTMLYHNEKD GIDVRIPAVI LFSKPGQLHL QWSVDDLDVE 

       670        680        690        700        710        720 
RQIVEFMFGR NDAWSNWGDF QPDRPVRSFL MVLRSIGGLF RRGQRLFRWQ APQEAPLAES 

       730        740        750        760        770        780 
EHVEEEKLEK KSLVLKPVRR SARHGATASL IVLLGLPAAI APSLAQAPSR ATPVATEQGA 

       790        800        810        820        830        840 
TPVEPPPVNA PPPPSLPQPP GTLPTPPQIA PASAGELLPA ATAVSLPTGP ATQQMRERLS 

       850        860        870        880        890        900 
ERTGVSPASP FGDTNTGALP ADPSAPPIDP ADAARVADGE ITRTSTFRDL GLATGPLTLR 

       910        920        930        940        950        960 
GFSPLQGLDV IVPANRVVTR ARITLSGALS PSLLPEASAV SVTLNEQYVG TIRVDPEHPR 

       970        980        990       1000       1010       1020 
FGPITFDIDP LYFTGDNKLN FHFAGEYRRD CNDLYNEVLW ARISDFSTVT LTTTRIAPDR 

      1030       1040       1050       1060       1070       1080 
KLSYLPAPFY DPNLRTPLRV PVVMPNPDAH GMLKASALVA SWFGKLADFR KVSFPVSTTI 

      1090       1100       1110       1120       1130       1140 
PASGNAIAIG ENLPIDARGT RPTGPTLSEV ENPNDRLGTI LVLTGRNAQE VEVAARVLAF 

      1150       1160       1170       1180       1190       1200 
SSDTLGAVGT KVVNDVTLQP RHPYDAPAFV PTDRPVRFGE LVAASDLQGG GFAPPVMALP 

      1210       1220       1230       1240       1250       1260 
FHLPPDLYSW RNRPYPIDLW VRTPGGPVVD LETSRLDVHL NNNYLDSFTL KPPSLWAAWS 

      1270       1280       1290       1300       1310       1320 
ERLVNQHAGA VEHAAALPPW LLFGQNQLKF SFDARPIDRG VCRRTPDDIH MSVDSDSWLD 

      1330       1340       1350       1360       1370       1380 
FRRGYHFARL PNLSYFAEAA FPFSRMADLS ETTVVVPHHI DAGTAGTFMD LMGFFGATTW 

      1390       1400       1410       1420       1430       1440 
YPASGVQVAD INDLSEHPPQ GDILILATAG DAPKFEELLT RAPYELTDGH IRVGQHMGLQ 

      1450       1460       1470       1480       1490       1500 
GIWYLFQDHD HAGLQDGVQA NLNAPIAGAG VLLGAQSPYR SDRSVVALMG DTPSRMHDLV 

      1510       1520       1530       1540       1550       1560 
MGLRSKEDVP RIQGDLVLRN GDRLTSYRTA PTFTMGSLPW WMWLDWYLGT RPLTLYVLGL 

      1570       1580       1590 
VGAGLVAAAA VRLLRRRAQH RLEEAARVKD TTDASH

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References

[1]"Identification of a second cellulose synthase gene (acsAII) in Acetobacter xylinum."
Saxena I.M., Brown R.M. Jr.
J. Bacteriol. 177:5276-5283(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23769 / NCIMB 8246.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U15957 Genomic DNA. Translation: AAA85264.1.
PIRT31338.

3D structure databases

ProteinModelPortalQ59167.
ModBaseSearch...

Protein family/group databases

CAZyGT2. Glycosyltransferase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003919. Cell_synth_A.
IPR003920. Cell_synth_B.
IPR018513. Cell_synthase_bac.
IPR005150. Cellulose_synth.
IPR001173. Glyco_trans_2.
IPR009875. PilZ_domain.
[Graphical view]
PfamPF03170. BcsB. 1 hit.
PF03552. Cellulose_synt. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF07238. PilZ. 1 hit.
[Graphical view]
PRINTSPR01440. CELLSNTHASEB.
TIGRFAMsTIGR03030. CelA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameACSA2_GLUHA
AccessionPrimary (citable) accession number: Q59167
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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