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Q59140

- BGAL_ARTSB

UniProt

Q59140 - BGAL_ARTSB

Protein

Beta-galactosidase

Gene

lacZ

Organism
Arthrobacter sp. (strain B7)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Cofactori

    Magnesium or manganese.

    pH dependencei

    Optimum pH is 7.2.

    Temperature dependencei

    Optimum temperature is 30 degrees Celsius with o-nitrophenyl-beta-D-galactopyranoside (ONPG) as substrate. Has high specific activity at low temperatures.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei434 – 4341Proton donorBy similarity
    Active sitei513 – 5131NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Manganese

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Lactase
    Gene namesi
    Name:lacZ
    OrganismiArthrobacter sp. (strain B7)
    Taxonomic identifieri86041 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10151015Beta-galactosidasePRO_0000057657Add
    BLAST

    Expressioni

    Inductioni

    By growth on lactose.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59140.
    SMRiQ59140. Positions 4-1015.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59140-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSYITDQG PGSGLRVPAR SWLNSDAPSL SLNGDWRFRL LPTAPGTPGA     50
    GSVLATGETV EAVASESFDD SSWDTLAVPS HWVLAEDGKY GRPIYTNVQY 100
    PFPIDPPFVP DANPTGDYRR TFDVPDSWFE STTAALTLRF DGVESRYKVW 150
    VNGVEIGVGS GSRLAQEFDV SEALRPGKNL LVVRVHQWSA ASYLEDQDQW 200
    WLPGIFRDVK LQARPVGGLT DVWLRTDWSG SGTITPEITA DPAAFPVTLR 250
    VPELGLEVIW DSPADVAPVS IDAVEPWSAE VPRLYDASVS SAAESISLRL 300
    GFRTVKIVGD QFLVNGRKVI FHGVNRHETN ADRGRVFDEA SAREDLALMK 350
    RFNVNAIRTS HYPPHPRFLD LADELGFWVI LECDLETHGF HALKWVGNPS 400
    DDPAWRDALV DRMERTVERD KNHASIVMWS LGNESGTGAN LAAMAAWTHA 450
    RDLSRPVHYE GDYTGAYTDV YSRMYSSIPE TDSIGRNDSH ALLLGCNAIE 500
    SARQRTRPFI LCEYVHAMGN GPGAIDQYED LVDKYPRLHG GFVWEWRDHG 550
    IRTRTADGTE FFAYGGDFDE VIHDGNFVMD GMILSDSTPT PGLFEYKQIV 600
    SPIRLALTLN AEGNAGLTVA NLRHTSDASD VVLRWRVEHN GTRVDAGELT 650
    TDGANGPLQA GDSLTLTLPT IVAAAEGETW LSVEAVLREA TAWAPAGHPL 700
    SETQLDLSPA QPPLRVPRPA SPIAGAAPVE LGPATFDAGS LVTLAGLPVA 750
    GPRLELWRAP TDNDKGQGFG AYGPEDPWIN SGRGVPAPSS AVVWQQAGLD 800
    RLTRRVEDVA ALPQGLRVRS RYAAANSEHD VAVEENWQLS GDELWLRIDI 850
    APSAGWDLVF PRIGVRLDLP SEVDGASWFG AGPRESYPDS LHSAVVGTHG 900
    GSLEELNVNY ARPQETGHHS DVRWVELSRD GAPWLRIEAD PDALGRRPGF 950
    SLAKNTAQEV ALAPHPHELP ESQHSYLYLD AAQHGLGSRA CGPDVWPDFA 1000
    LRPEARTLVL RIRAA 1015
    Length:1,015
    Mass (Da):110,903
    Last modified:November 1, 1996 - v1
    Checksum:i7846E36D62F76302
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12334 Genomic DNA. Translation: AAA69907.1.
    PIRiI39697.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12334 Genomic DNA. Translation: AAA69907.1 .
    PIRi I39697.

    3D structure databases

    ProteinModelPortali Q59140.
    SMRi Q59140. Positions 4-1015.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase."
      Trimbur D.E., Gutshall K.R., Prema P., Brenchley J.E.
      Appl. Environ. Microbiol. 60:4544-4552(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, CHARACTERIZATION.

    Entry informationi

    Entry nameiBGAL_ARTSB
    AccessioniPrimary (citable) accession number: Q59140
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3