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Protein

Beta-galactosidase

Gene

lacZ

Organism
Arthrobacter sp. (strain B7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Cofactori

pH dependencei

Optimum pH is 7.2.

Temperature dependencei

Optimum temperature is 30 degrees Celsius with o-nitrophenyl-beta-D-galactopyranoside (ONPG) as substrate. Has high specific activity at low temperatures.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei434 – 4341Proton donorBy similarity
Active sitei513 – 5131NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Manganese

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiArthrobacter sp. (strain B7)
Taxonomic identifieri86041 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10151015Beta-galactosidasePRO_0000057657Add
BLAST

Expressioni

Inductioni

By growth on lactose.

Structurei

3D structure databases

ProteinModelPortaliQ59140.
SMRiQ59140. Positions 4-1015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSYITDQG PGSGLRVPAR SWLNSDAPSL SLNGDWRFRL LPTAPGTPGA
60 70 80 90 100
GSVLATGETV EAVASESFDD SSWDTLAVPS HWVLAEDGKY GRPIYTNVQY
110 120 130 140 150
PFPIDPPFVP DANPTGDYRR TFDVPDSWFE STTAALTLRF DGVESRYKVW
160 170 180 190 200
VNGVEIGVGS GSRLAQEFDV SEALRPGKNL LVVRVHQWSA ASYLEDQDQW
210 220 230 240 250
WLPGIFRDVK LQARPVGGLT DVWLRTDWSG SGTITPEITA DPAAFPVTLR
260 270 280 290 300
VPELGLEVIW DSPADVAPVS IDAVEPWSAE VPRLYDASVS SAAESISLRL
310 320 330 340 350
GFRTVKIVGD QFLVNGRKVI FHGVNRHETN ADRGRVFDEA SAREDLALMK
360 370 380 390 400
RFNVNAIRTS HYPPHPRFLD LADELGFWVI LECDLETHGF HALKWVGNPS
410 420 430 440 450
DDPAWRDALV DRMERTVERD KNHASIVMWS LGNESGTGAN LAAMAAWTHA
460 470 480 490 500
RDLSRPVHYE GDYTGAYTDV YSRMYSSIPE TDSIGRNDSH ALLLGCNAIE
510 520 530 540 550
SARQRTRPFI LCEYVHAMGN GPGAIDQYED LVDKYPRLHG GFVWEWRDHG
560 570 580 590 600
IRTRTADGTE FFAYGGDFDE VIHDGNFVMD GMILSDSTPT PGLFEYKQIV
610 620 630 640 650
SPIRLALTLN AEGNAGLTVA NLRHTSDASD VVLRWRVEHN GTRVDAGELT
660 670 680 690 700
TDGANGPLQA GDSLTLTLPT IVAAAEGETW LSVEAVLREA TAWAPAGHPL
710 720 730 740 750
SETQLDLSPA QPPLRVPRPA SPIAGAAPVE LGPATFDAGS LVTLAGLPVA
760 770 780 790 800
GPRLELWRAP TDNDKGQGFG AYGPEDPWIN SGRGVPAPSS AVVWQQAGLD
810 820 830 840 850
RLTRRVEDVA ALPQGLRVRS RYAAANSEHD VAVEENWQLS GDELWLRIDI
860 870 880 890 900
APSAGWDLVF PRIGVRLDLP SEVDGASWFG AGPRESYPDS LHSAVVGTHG
910 920 930 940 950
GSLEELNVNY ARPQETGHHS DVRWVELSRD GAPWLRIEAD PDALGRRPGF
960 970 980 990 1000
SLAKNTAQEV ALAPHPHELP ESQHSYLYLD AAQHGLGSRA CGPDVWPDFA
1010
LRPEARTLVL RIRAA
Length:1,015
Mass (Da):110,903
Last modified:November 1, 1996 - v1
Checksum:i7846E36D62F76302
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12334 Genomic DNA. Translation: AAA69907.1.
PIRiI39697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12334 Genomic DNA. Translation: AAA69907.1.
PIRiI39697.

3D structure databases

ProteinModelPortaliQ59140.
SMRiQ59140. Positions 4-1015.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase."
    Trimbur D.E., Gutshall K.R., Prema P., Brenchley J.E.
    Appl. Environ. Microbiol. 60:4544-4552(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, CHARACTERIZATION.

Entry informationi

Entry nameiBGAL_ARTSB
AccessioniPrimary (citable) accession number: Q59140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.