Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q59140 (BGAL_ARTSB) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismArthrobacter sp. (strain B7)
Taxonomic identifier86041 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length1015 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Cofactor

Magnesium or manganese.

Induction

By growth on lactose.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.2.

Temperature dependence:

Optimum temperature is 30 degrees Celsius with o-nitrophenyl-beta-D-galactopyranoside (ONPG) as substrate. Has high specific activity at low temperatures.

Ontologies

Keywords
   LigandMagnesium
Manganese
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10151015Beta-galactosidase
PRO_0000057657

Sites

Active site4341Proton donor By similarity
Active site5131Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q59140 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7846E36D62F76302

FASTA1,015110,903
        10         20         30         40         50         60 
MSSSYITDQG PGSGLRVPAR SWLNSDAPSL SLNGDWRFRL LPTAPGTPGA GSVLATGETV 

        70         80         90        100        110        120 
EAVASESFDD SSWDTLAVPS HWVLAEDGKY GRPIYTNVQY PFPIDPPFVP DANPTGDYRR 

       130        140        150        160        170        180 
TFDVPDSWFE STTAALTLRF DGVESRYKVW VNGVEIGVGS GSRLAQEFDV SEALRPGKNL 

       190        200        210        220        230        240 
LVVRVHQWSA ASYLEDQDQW WLPGIFRDVK LQARPVGGLT DVWLRTDWSG SGTITPEITA 

       250        260        270        280        290        300 
DPAAFPVTLR VPELGLEVIW DSPADVAPVS IDAVEPWSAE VPRLYDASVS SAAESISLRL 

       310        320        330        340        350        360 
GFRTVKIVGD QFLVNGRKVI FHGVNRHETN ADRGRVFDEA SAREDLALMK RFNVNAIRTS 

       370        380        390        400        410        420 
HYPPHPRFLD LADELGFWVI LECDLETHGF HALKWVGNPS DDPAWRDALV DRMERTVERD 

       430        440        450        460        470        480 
KNHASIVMWS LGNESGTGAN LAAMAAWTHA RDLSRPVHYE GDYTGAYTDV YSRMYSSIPE 

       490        500        510        520        530        540 
TDSIGRNDSH ALLLGCNAIE SARQRTRPFI LCEYVHAMGN GPGAIDQYED LVDKYPRLHG 

       550        560        570        580        590        600 
GFVWEWRDHG IRTRTADGTE FFAYGGDFDE VIHDGNFVMD GMILSDSTPT PGLFEYKQIV 

       610        620        630        640        650        660 
SPIRLALTLN AEGNAGLTVA NLRHTSDASD VVLRWRVEHN GTRVDAGELT TDGANGPLQA 

       670        680        690        700        710        720 
GDSLTLTLPT IVAAAEGETW LSVEAVLREA TAWAPAGHPL SETQLDLSPA QPPLRVPRPA 

       730        740        750        760        770        780 
SPIAGAAPVE LGPATFDAGS LVTLAGLPVA GPRLELWRAP TDNDKGQGFG AYGPEDPWIN 

       790        800        810        820        830        840 
SGRGVPAPSS AVVWQQAGLD RLTRRVEDVA ALPQGLRVRS RYAAANSEHD VAVEENWQLS 

       850        860        870        880        890        900 
GDELWLRIDI APSAGWDLVF PRIGVRLDLP SEVDGASWFG AGPRESYPDS LHSAVVGTHG 

       910        920        930        940        950        960 
GSLEELNVNY ARPQETGHHS DVRWVELSRD GAPWLRIEAD PDALGRRPGF SLAKNTAQEV 

       970        980        990       1000       1010 
ALAPHPHELP ESQHSYLYLD AAQHGLGSRA CGPDVWPDFA LRPEARTLVL RIRAA 

« Hide

References

[1]"Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase."
Trimbur D.E., Gutshall K.R., Prema P., Brenchley J.E.
Appl. Environ. Microbiol. 60:4544-4552(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12334 Genomic DNA. Translation: AAA69907.1.
PIRI39697.

3D structure databases

ProteinModelPortalQ59140.
SMRQ59140. Positions 4-1015.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_ARTSB
AccessionPrimary (citable) accession number: Q59140
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries