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Q59140

- BGAL_ARTSB

UniProt

Q59140 - BGAL_ARTSB

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Protein

Beta-galactosidase

Gene
lacZ
Organism
Arthrobacter sp. (strain B7)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Cofactori

Magnesium or manganese.

pH dependencei

Optimum pH is 7.2.

Temperature dependencei

Optimum temperature is 30 degrees Celsius with o-nitrophenyl-beta-D-galactopyranoside (ONPG) as substrate. Has high specific activity at low temperatures.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei434 – 4341Proton donor By similarity
Active sitei513 – 5131Nucleophile By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Manganese

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiArthrobacter sp. (strain B7)
Taxonomic identifieri86041 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10151015Beta-galactosidasePRO_0000057657Add
BLAST

Expressioni

Inductioni

By growth on lactose.

Structurei

3D structure databases

ProteinModelPortaliQ59140.
SMRiQ59140. Positions 4-1015.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59140-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSYITDQG PGSGLRVPAR SWLNSDAPSL SLNGDWRFRL LPTAPGTPGA     50
GSVLATGETV EAVASESFDD SSWDTLAVPS HWVLAEDGKY GRPIYTNVQY 100
PFPIDPPFVP DANPTGDYRR TFDVPDSWFE STTAALTLRF DGVESRYKVW 150
VNGVEIGVGS GSRLAQEFDV SEALRPGKNL LVVRVHQWSA ASYLEDQDQW 200
WLPGIFRDVK LQARPVGGLT DVWLRTDWSG SGTITPEITA DPAAFPVTLR 250
VPELGLEVIW DSPADVAPVS IDAVEPWSAE VPRLYDASVS SAAESISLRL 300
GFRTVKIVGD QFLVNGRKVI FHGVNRHETN ADRGRVFDEA SAREDLALMK 350
RFNVNAIRTS HYPPHPRFLD LADELGFWVI LECDLETHGF HALKWVGNPS 400
DDPAWRDALV DRMERTVERD KNHASIVMWS LGNESGTGAN LAAMAAWTHA 450
RDLSRPVHYE GDYTGAYTDV YSRMYSSIPE TDSIGRNDSH ALLLGCNAIE 500
SARQRTRPFI LCEYVHAMGN GPGAIDQYED LVDKYPRLHG GFVWEWRDHG 550
IRTRTADGTE FFAYGGDFDE VIHDGNFVMD GMILSDSTPT PGLFEYKQIV 600
SPIRLALTLN AEGNAGLTVA NLRHTSDASD VVLRWRVEHN GTRVDAGELT 650
TDGANGPLQA GDSLTLTLPT IVAAAEGETW LSVEAVLREA TAWAPAGHPL 700
SETQLDLSPA QPPLRVPRPA SPIAGAAPVE LGPATFDAGS LVTLAGLPVA 750
GPRLELWRAP TDNDKGQGFG AYGPEDPWIN SGRGVPAPSS AVVWQQAGLD 800
RLTRRVEDVA ALPQGLRVRS RYAAANSEHD VAVEENWQLS GDELWLRIDI 850
APSAGWDLVF PRIGVRLDLP SEVDGASWFG AGPRESYPDS LHSAVVGTHG 900
GSLEELNVNY ARPQETGHHS DVRWVELSRD GAPWLRIEAD PDALGRRPGF 950
SLAKNTAQEV ALAPHPHELP ESQHSYLYLD AAQHGLGSRA CGPDVWPDFA 1000
LRPEARTLVL RIRAA 1015
Length:1,015
Mass (Da):110,903
Last modified:November 1, 1996 - v1
Checksum:i7846E36D62F76302
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12334 Genomic DNA. Translation: AAA69907.1.
PIRiI39697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12334 Genomic DNA. Translation: AAA69907.1 .
PIRi I39697.

3D structure databases

ProteinModelPortali Q59140.
SMRi Q59140. Positions 4-1015.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase."
    Trimbur D.E., Gutshall K.R., Prema P., Brenchley J.E.
    Appl. Environ. Microbiol. 60:4544-4552(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, CHARACTERIZATION.

Entry informationi

Entry nameiBGAL_ARTSB
AccessioniPrimary (citable) accession number: Q59140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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