Reviewed, UniProtKB/Swiss-Prot Q59140 (BGAL_ARTSB)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase

Gene names

Name:

lacZ

Organism

Arthrobacter sp. (strain B7)

Taxonomic identifier

86041 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	1015 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Cofactor	Magnesium or manganese.
Induction	By growth on lactose.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.
biophysicochemical properties	pH dependence: Optimum pH is 7.2. Temperature dependence: Optimum temperature is 30 degrees Celsius with o-nitrophenyl-beta-D-galactopyranoside (ONPG) as substrate. Has high specific activity at low temperatures.

Ontologies

Keywords
Ligand	Magnesium Manganese
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1015	1015	Beta-galactosidase		PRO_0000057657
Sites
	Active site	434	1	Proton donor By similarity
	Active site	513	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59140-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7846E36D62F76302	FASTA	1,015	110,903
	10 20 30 40 50 60 MSSSYITDQG PGSGLRVPAR SWLNSDAPSL SLNGDWRFRL LPTAPGTPGA GSVLATGETV 70 80 90 100 110 120 EAVASESFDD SSWDTLAVPS HWVLAEDGKY GRPIYTNVQY PFPIDPPFVP DANPTGDYRR 130 140 150 160 170 180 TFDVPDSWFE STTAALTLRF DGVESRYKVW VNGVEIGVGS GSRLAQEFDV SEALRPGKNL 190 200 210 220 230 240 LVVRVHQWSA ASYLEDQDQW WLPGIFRDVK LQARPVGGLT DVWLRTDWSG SGTITPEITA 250 260 270 280 290 300 DPAAFPVTLR VPELGLEVIW DSPADVAPVS IDAVEPWSAE VPRLYDASVS SAAESISLRL 310 320 330 340 350 360 GFRTVKIVGD QFLVNGRKVI FHGVNRHETN ADRGRVFDEA SAREDLALMK RFNVNAIRTS 370 380 390 400 410 420 HYPPHPRFLD LADELGFWVI LECDLETHGF HALKWVGNPS DDPAWRDALV DRMERTVERD 430 440 450 460 470 480 KNHASIVMWS LGNESGTGAN LAAMAAWTHA RDLSRPVHYE GDYTGAYTDV YSRMYSSIPE 490 500 510 520 530 540 TDSIGRNDSH ALLLGCNAIE SARQRTRPFI LCEYVHAMGN GPGAIDQYED LVDKYPRLHG 550 560 570 580 590 600 GFVWEWRDHG IRTRTADGTE FFAYGGDFDE VIHDGNFVMD GMILSDSTPT PGLFEYKQIV 610 620 630 640 650 660 SPIRLALTLN AEGNAGLTVA NLRHTSDASD VVLRWRVEHN GTRVDAGELT TDGANGPLQA 670 680 690 700 710 720 GDSLTLTLPT IVAAAEGETW LSVEAVLREA TAWAPAGHPL SETQLDLSPA QPPLRVPRPA 730 740 750 760 770 780 SPIAGAAPVE LGPATFDAGS LVTLAGLPVA GPRLELWRAP TDNDKGQGFG AYGPEDPWIN 790 800 810 820 830 840 SGRGVPAPSS AVVWQQAGLD RLTRRVEDVA ALPQGLRVRS RYAAANSEHD VAVEENWQLS 850 860 870 880 890 900 GDELWLRIDI APSAGWDLVF PRIGVRLDLP SEVDGASWFG AGPRESYPDS LHSAVVGTHG 910 920 930 940 950 960 GSLEELNVNY ARPQETGHHS DVRWVELSRD GAPWLRIEAD PDALGRRPGF SLAKNTAQEV 970 980 990 1000 1010 ALAPHPHELP ESQHSYLYLD AAQHGLGSRA CGPDVWPDFA LRPEARTLVL RIRAA

References

[1]

"Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase." Trimbur D.E., Gutshall K.R., Prema P., Brenchley J.E. Appl. Environ. Microbiol. 60:4544-4552(1994) [PubMed: 7811090] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12334 Genomic DNA. Translation: AAA69907.1.
PIR	I39697.
3D structure databases
HSSP	HSSP built from PDB template 1JZ7 based on UniProtKB P00722.
SMR	Q59140. Positions 4-1015.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Family and domain databases
InterPro	IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_ARTSB

Accession

Primary (citable) accession number: Q59140

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)