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Q59112

- GCTB_ACIFV

UniProt

Q59112 - GCTB_ACIFV

Protein

Glutaconate CoA-transferase subunit B

Gene

gctB

Organism
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of the CoA moiety from acetyl-CoA to (R)-2-hydroxyglutarate and related compounds like glutaconate.

    Catalytic activityi

    Acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei54 – 541

    GO - Molecular functioni

    1. glutaconate CoA-transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamate catabolic process via 2-hydroxyglutarate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciAFER591001:GHUL-1891-MONOMER.
    MetaCyc:MONOMER-1029.
    SABIO-RKQ59112.
    UniPathwayiUPA00533; UER00686.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaconate CoA-transferase subunit B (EC:2.8.3.12)
    Alternative name(s):
    GCT small subunit
    Gene namesi
    Name:gctB
    Ordered Locus Names:Acfer_1818
    OrganismiAcidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
    Taxonomic identifieri591001 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesAcidaminococcaceaeAcidaminococcus
    ProteomesiUP000001902: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 266265Glutaconate CoA-transferase subunit BPRO_0000157930Add
    BLAST

    Interactioni

    Subunit structurei

    Heterooctamer of four A and four B subunits.

    Protein-protein interaction databases

    DIPiDIP-6201N.
    IntActiQ59112. 1 interaction.

    Structurei

    Secondary structure

    1
    266
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 1810
    Beta strandi26 – 283
    Helixi33 – 4311
    Beta strandi50 – 534
    Turni54 – 563
    Beta strandi57 – 604
    Helixi72 – 754
    Beta strandi77 – 815
    Helixi84 – 9714
    Beta strandi102 – 1065
    Beta strandi109 – 1113
    Beta strandi120 – 1234
    Beta strandi125 – 1317
    Helixi138 – 1447
    Beta strandi147 – 1504
    Turni155 – 1573
    Helixi176 – 1794
    Beta strandi188 – 1936
    Beta strandi196 – 2005
    Turni202 – 2043
    Beta strandi207 – 2126
    Helixi218 – 2236
    Helixi242 – 2509

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1POIX-ray2.50B/D3-262[»]
    ProteinModelPortaliQ59112.
    SMRiQ59112. Positions 3-262.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ59112.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000221246.
    KOiK01040.
    OMAiHSKRTFV.

    Family and domain databases

    InterProiIPR004165. CoA_trans_fam_I.
    [Graphical view]
    PANTHERiPTHR13707. PTHR13707. 1 hit.
    PfamiPF01144. CoA_trans. 1 hit.
    [Graphical view]
    SMARTiSM00882. CoA_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q59112-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADYTNYTNK EMQAVTIAKQ IKNGQVVTVG TGLPLIGASV AKRVYAPDCH    50
    IIVESGLMDC SPVEVPRSVG DLRFMAHCGC IWPNVRFVGF EINEYLHKAN 100
    RLIAFIGGAQ IDPYGNVNST SIGDYHHPKT RFTGSGGANG IATYSNTIIM 150
    MQHEKRRFMN KIDYVTSPGW IDGPGGRERL GLPGDVGPQL VVTDKGILKF 200
    DEKTKRMYLA AYYPTSSPED VLENTGFDLD VSKAVELEAP DPAVIKLIRE 250
    EIDPGQAFIQ VPTEAK 266
    Length:266
    Mass (Da):29,166
    Last modified:January 23, 2007 - v3
    Checksum:i1E691B864237A784
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81440 Genomic DNA. Translation: CAA57200.1.
    CP001859 Genomic DNA. Translation: ADB48172.1.
    PIRiS51052.
    RefSeqiWP_012939155.1. NC_013740.1.
    YP_003399487.1. NC_013740.1.

    Genome annotation databases

    EnsemblBacteriaiADB48172; ADB48172; Acfer_1818.
    GeneIDi8738321.
    KEGGiafn:Acfer_1818.
    PATRICi31910227. VBIAciFer109666_1809.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81440 Genomic DNA. Translation: CAA57200.1 .
    CP001859 Genomic DNA. Translation: ADB48172.1 .
    PIRi S51052.
    RefSeqi WP_012939155.1. NC_013740.1.
    YP_003399487.1. NC_013740.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1POI X-ray 2.50 B/D 3-262 [» ]
    ProteinModelPortali Q59112.
    SMRi Q59112. Positions 3-262.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6201N.
    IntActi Q59112. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADB48172 ; ADB48172 ; Acfer_1818 .
    GeneIDi 8738321.
    KEGGi afn:Acfer_1818.
    PATRICi 31910227. VBIAciFer109666_1809.

    Phylogenomic databases

    HOGENOMi HOG000221246.
    KOi K01040.
    OMAi HSKRTFV.

    Enzyme and pathway databases

    UniPathwayi UPA00533 ; UER00686 .
    BioCyci AFER591001:GHUL-1891-MONOMER.
    MetaCyc:MONOMER-1029.
    SABIO-RK Q59112.

    Miscellaneous databases

    EvolutionaryTracei Q59112.

    Family and domain databases

    InterProi IPR004165. CoA_trans_fam_I.
    [Graphical view ]
    PANTHERi PTHR13707. PTHR13707. 1 hit.
    Pfami PF01144. CoA_trans. 1 hit.
    [Graphical view ]
    SMARTi SM00882. CoA_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Location of the two genes encoding glutaconate coenzyme A-transferase at the beginning of the hydroxyglutarate operon in Acidaminococcus fermentans."
      Mack M., Bendrat K., Zelder O., Eckel E., Linder D., Buckel W.
      Eur. J. Biochem. 226:41-51(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-19 AND 44-66.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25085 / DSM 20731 / VR4.
    3. "Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases."
      Jacob U., Mack M., Clausen T., Huber R., Buckel W., Messerschmidt A.
      Structure 5:415-426(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).

    Entry informationi

    Entry nameiGCTB_ACIFV
    AccessioniPrimary (citable) accession number: Q59112
    Secondary accession number(s): D2RM70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3