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Q59112

- GCTB_ACIFV

UniProt

Q59112 - GCTB_ACIFV

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Protein

Glutaconate CoA-transferase subunit B

Gene

gctB

Organism
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the CoA moiety from acetyl-CoA to (R)-2-hydroxyglutarate and related compounds like glutaconate.

Catalytic activityi

Acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 541

GO - Molecular functioni

  1. glutaconate CoA-transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamate catabolic process via 2-hydroxyglutarate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciAFER591001:GHUL-1891-MONOMER.
MetaCyc:MONOMER-1029.
SABIO-RKQ59112.
UniPathwayiUPA00533; UER00686.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaconate CoA-transferase subunit B (EC:2.8.3.12)
Alternative name(s):
GCT small subunit
Gene namesi
Name:gctB
Ordered Locus Names:Acfer_1818
OrganismiAcidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Taxonomic identifieri591001 [NCBI]
Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesAcidaminococcaceaeAcidaminococcus
ProteomesiUP000001902: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 266265Glutaconate CoA-transferase subunit BPRO_0000157930Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of four A and four B subunits.

Protein-protein interaction databases

DIPiDIP-6201N.
IntActiQ59112. 1 interaction.

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 1810
Beta strandi26 – 283
Helixi33 – 4311
Beta strandi50 – 534
Turni54 – 563
Beta strandi57 – 604
Helixi72 – 754
Beta strandi77 – 815
Helixi84 – 9714
Beta strandi102 – 1065
Beta strandi109 – 1113
Beta strandi120 – 1234
Beta strandi125 – 1317
Helixi138 – 1447
Beta strandi147 – 1504
Turni155 – 1573
Helixi176 – 1794
Beta strandi188 – 1936
Beta strandi196 – 2005
Turni202 – 2043
Beta strandi207 – 2126
Helixi218 – 2236
Helixi242 – 2509

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1POIX-ray2.50B/D3-262[»]
ProteinModelPortaliQ59112.
SMRiQ59112. Positions 3-262.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59112.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000221246.
KOiK01040.
OMAiHSKRTFV.

Family and domain databases

InterProiIPR004165. CoA_trans_fam_I.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTiSM00882. CoA_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59112-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADYTNYTNK EMQAVTIAKQ IKNGQVVTVG TGLPLIGASV AKRVYAPDCH
60 70 80 90 100
IIVESGLMDC SPVEVPRSVG DLRFMAHCGC IWPNVRFVGF EINEYLHKAN
110 120 130 140 150
RLIAFIGGAQ IDPYGNVNST SIGDYHHPKT RFTGSGGANG IATYSNTIIM
160 170 180 190 200
MQHEKRRFMN KIDYVTSPGW IDGPGGRERL GLPGDVGPQL VVTDKGILKF
210 220 230 240 250
DEKTKRMYLA AYYPTSSPED VLENTGFDLD VSKAVELEAP DPAVIKLIRE
260
EIDPGQAFIQ VPTEAK
Length:266
Mass (Da):29,166
Last modified:January 23, 2007 - v3
Checksum:i1E691B864237A784
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81440 Genomic DNA. Translation: CAA57200.1.
CP001859 Genomic DNA. Translation: ADB48172.1.
PIRiS51052.
RefSeqiWP_012939155.1. NC_013740.1.
YP_003399487.1. NC_013740.1.

Genome annotation databases

EnsemblBacteriaiADB48172; ADB48172; Acfer_1818.
GeneIDi8738321.
KEGGiafn:Acfer_1818.
PATRICi31910227. VBIAciFer109666_1809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81440 Genomic DNA. Translation: CAA57200.1 .
CP001859 Genomic DNA. Translation: ADB48172.1 .
PIRi S51052.
RefSeqi WP_012939155.1. NC_013740.1.
YP_003399487.1. NC_013740.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1POI X-ray 2.50 B/D 3-262 [» ]
ProteinModelPortali Q59112.
SMRi Q59112. Positions 3-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6201N.
IntActi Q59112. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADB48172 ; ADB48172 ; Acfer_1818 .
GeneIDi 8738321.
KEGGi afn:Acfer_1818.
PATRICi 31910227. VBIAciFer109666_1809.

Phylogenomic databases

HOGENOMi HOG000221246.
KOi K01040.
OMAi HSKRTFV.

Enzyme and pathway databases

UniPathwayi UPA00533 ; UER00686 .
BioCyci AFER591001:GHUL-1891-MONOMER.
MetaCyc:MONOMER-1029.
SABIO-RK Q59112.

Miscellaneous databases

EvolutionaryTracei Q59112.

Family and domain databases

InterProi IPR004165. CoA_trans_fam_I.
[Graphical view ]
PANTHERi PTHR13707. PTHR13707. 1 hit.
Pfami PF01144. CoA_trans. 1 hit.
[Graphical view ]
SMARTi SM00882. CoA_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Location of the two genes encoding glutaconate coenzyme A-transferase at the beginning of the hydroxyglutarate operon in Acidaminococcus fermentans."
    Mack M., Bendrat K., Zelder O., Eckel E., Linder D., Buckel W.
    Eur. J. Biochem. 226:41-51(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-19 AND 44-66.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25085 / DSM 20731 / VR4.
  3. "Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases."
    Jacob U., Mack M., Clausen T., Huber R., Buckel W., Messerschmidt A.
    Structure 5:415-426(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).

Entry informationi

Entry nameiGCTB_ACIFV
AccessioniPrimary (citable) accession number: Q59112
Secondary accession number(s): D2RM70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3