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Protein

Sulfite reductase, dissimilatory-type subunit beta

Gene

dsrB

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of sulfite to sulfide. This is the terminal oxidation reaction in sulfate respiration.1 Publication

Catalytic activityi

Hydrogen sulfide + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O = sulfite + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+.2 Publications
A [DsrC protein]-S-sulfanyl-L-cysteine + 3 acceptor + 3 H2O = sulfite + a [DsrC protein]-disulfide + 3 reduced acceptor + 2 H+.2 Publications

Cofactori

Protein has several cofactor binding sites:

Temperature dependencei

Highly thermostable. Inactive towards methylviologen below 55 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi140Iron-sulfur (4Fe-4S) 1Combined sources2 Publications1
Metal bindingi177Iron-sulfur (4Fe-4S) 1Combined sources2 Publications1
Metal bindingi178Iron-sulfur (4Fe-4S) 1Combined sources2 Publications1
Metal bindingi182Iron (siroheme axial ligand)2 Publications1
Metal bindingi182Iron-sulfur (4Fe-4S) 1Combined sources2 Publications1
Metal bindingi220Iron-sulfur (4Fe-4S) 2Combined sources2 Publications1
Metal bindingi241Iron-sulfur (4Fe-4S) 2Combined sources2 Publications1
Metal bindingi244Iron-sulfur (4Fe-4S) 2Combined sources2 Publications1
Metal bindingi247Iron-sulfur (4Fe-4S) 2Combined sources2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12501.
BRENDAi1.8.99.3. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase, dissimilatory-type subunit beta (EC:1.8.99.52 Publications)
Alternative name(s):
Hydrogensulfite reductase subunit beta
Gene namesi
Name:dsrB
Ordered Locus Names:AF_0424
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Membrane

  • Note: Although the protein complex is found in the soluble fraction it may be membrane-associated in vivo.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800271 – 366Sulfite reductase, dissimilatory-type subunit betaAdd BLAST366

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.2 Publications

Protein-protein interaction databases

STRINGi224325.AF0424.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 18Combined sources4
Helixi21 – 26Combined sources6
Beta strandi30 – 37Combined sources8
Beta strandi40 – 45Combined sources6
Beta strandi50 – 56Combined sources7
Beta strandi60 – 63Combined sources4
Helixi64 – 77Combined sources14
Beta strandi81 – 84Combined sources4
Beta strandi90 – 95Combined sources6
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Turni121 – 124Combined sources4
Helixi136 – 139Combined sources4
Helixi148 – 157Combined sources10
Helixi159 – 163Combined sources5
Beta strandi167 – 169Combined sources3
Beta strandi173 – 178Combined sources6
Beta strandi182 – 184Combined sources3
Helixi185 – 187Combined sources3
Beta strandi188 – 195Combined sources8
Helixi204 – 210Combined sources7
Helixi213 – 218Combined sources6
Beta strandi225 – 228Combined sources4
Turni229 – 232Combined sources4
Beta strandi233 – 236Combined sources4
Helixi238 – 240Combined sources3
Helixi246 – 250Combined sources5
Turni259 – 261Combined sources3
Beta strandi263 – 268Combined sources6
Beta strandi275 – 277Combined sources3
Beta strandi283 – 290Combined sources8
Turni293 – 295Combined sources3
Helixi297 – 313Combined sources17
Helixi320 – 327Combined sources8
Helixi329 – 336Combined sources8
Helixi342 – 344Combined sources3
Beta strandi350 – 352Combined sources3
Helixi353 – 357Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MM5X-ray1.80B/E1-366[»]
3MM6X-ray1.90B/E1-366[»]
3MM7X-ray1.90B/E1-366[»]
3MM8X-ray2.28B/E1-366[»]
3MM9X-ray2.10B/E1-366[»]
3MMAX-ray2.30B/E1-366[»]
3MMBX-ray2.30B/E1-366[»]
3MMCX-ray2.04B/E1-366[»]
ProteinModelPortaliQ59110.
SMRiQ59110.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59110.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 2624Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST31

Sequence similaritiesi

Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG02059. Archaea.
COG2221. LUCA.
KOiK11181.
OMAiWLHCDIP.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR011808. DsrB.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
[Graphical view]
PfamiPF00037. Fer4. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
[Graphical view]
SUPFAMiSSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02066. dsrB. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVEGVKTDF GPPYFRDLLH PVIAKNYGKW KYHEVVKPGV IKRVAESGDV
60 70 80 90 100
IYVVRFGTPR LLSIYTVREL CDIADKYSDG YLRWTSRNNV EFFVTDESKI
110 120 130 140 150
DDLINEVQER VGFPCGGTWD AVKGEYGLSN IVHTQGWIHC HTPAIDASGI
160 170 180 190 200
VKAVMDELYE YFTDHKLPAM CRISLACCAN MCGAVHASDI AIVGIHRTPP
210 220 230 240 250
IPNDEAIRKT CEIPSTVAAC PTGALKPDMK NKTIKVDVEK CMYCGNCYTM
260 270 280 290 300
CPGMPLFDPE NDGAAIMVGG KLSEARRMPE LSKVVVPWVP NEPPRWPTLV
310 320 330 340 350
KYVKQILEAW AANANKHERL IEWVDRIGWE RFFELTGLEF TQHLIDDYRI
360
TPYFYSEFRA STQFKW
Length:366
Mass (Da):41,570
Last modified:November 1, 1996 - v1
Checksum:iBA8E4C59216459DA
GO

Sequence cautioni

The sequence AAB90811 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95624 Genomic DNA. Translation: AAB17214.1.
AE000782 Genomic DNA. Translation: AAB90811.1. Different initiation.
PIRiH69302.
RefSeqiWP_048064231.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90811; AAB90811; AF_0424.
GeneIDi24793962.
KEGGiafu:AF_0424.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95624 Genomic DNA. Translation: AAB17214.1.
AE000782 Genomic DNA. Translation: AAB90811.1. Different initiation.
PIRiH69302.
RefSeqiWP_048064231.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MM5X-ray1.80B/E1-366[»]
3MM6X-ray1.90B/E1-366[»]
3MM7X-ray1.90B/E1-366[»]
3MM8X-ray2.28B/E1-366[»]
3MM9X-ray2.10B/E1-366[»]
3MMAX-ray2.30B/E1-366[»]
3MMBX-ray2.30B/E1-366[»]
3MMCX-ray2.04B/E1-366[»]
ProteinModelPortaliQ59110.
SMRiQ59110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90811; AAB90811; AF_0424.
GeneIDi24793962.
KEGGiafu:AF_0424.

Phylogenomic databases

eggNOGiarCOG02059. Archaea.
COG2221. LUCA.
KOiK11181.
OMAiWLHCDIP.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12501.
BRENDAi1.8.99.3. 414.

Miscellaneous databases

EvolutionaryTraceiQ59110.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR011808. DsrB.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
[Graphical view]
PfamiPF00037. Fer4. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
[Graphical view]
SUPFAMiSSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02066. dsrB. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDSRB_ARCFU
AccessioniPrimary (citable) accession number: Q59110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.