Reviewed, UniProtKB/Swiss-Prot Q59110 (DSRB_ARCFU)

Names and origin

Protein names

Recommended name:
    Sulfite reductase, dissimilatory-type subunit beta
    EC=1.8.99.3
Alternative name(s):
    Hydrogensulfite reductase subunit beta

Gene names

Name:	dsrB
Ordered Locus Names:	AF_0424

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reduction of sulfite to sulfide. This is the terminal oxidation reaction in sulfate respiration.
Catalytic activity	(O3S.S.SO3)2- + acceptor + 2 H2O + OH- = 3 HSO3- + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster per subunit. Binds 2 sirohemes per subunit.
Subunit structure	Heterotetramer of two alpha and two beta subunits.
Subcellular location	Membrane. Note: Although the protein complex is found in the soluble fraction it may be membrane-associated in vivo.
Sequence similarities	Contains 1 4Fe-4S ferredoxin-type domain.
Biophysicochemical properties	Temperature dependence: Highly thermostable. Inactive towards methylviologen below 55 degrees Celsius.

Ontologies

Keywords
Cellular component	Membrane
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro hydrogensulfite reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 366

366

Sulfite reductase, dissimilatory-type subunit beta

PRO_0000080027

Regions

Domain

232 – 262

31

4Fe-4S ferredoxin-type

Sites

Metal binding

140

1

Iron (heme axial ligand) Potential

Metal binding

178

1

Iron (heme axial ligand) Potential

Metal binding

182

1

Iron (heme axial ligand) Potential

Metal binding

220

1

Iron-sulfur (4Fe-4S) Potential

Metal binding

241

1

Iron-sulfur (4Fe-4S) Potential

Metal binding

244

1

Iron-sulfur (4Fe-4S) Potential

Metal binding

247

1

Iron-sulfur (4Fe-4S) Potential

Secondary structure

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366

Helix Strand Turn

Details...

Helix

15 – 18

4

Helix

21 – 26

6

Beta strand

30 – 37

8

Beta strand

40 – 45

6

Beta strand

50 – 56

7

Beta strand

60 – 63

4

Helix

64 – 77

14

Beta strand

81 – 84

4

Beta strand

90 – 95

6

Helix

97 – 99

3

Helix

100 – 111

12

Turn

121 – 124

4

Helix

136 – 139

4

Helix

148 – 162

15

Beta strand

167 – 169

3

Beta strand

173 – 178

6

Helix

185 – 187

3

Beta strand

189 – 195

7

Helix

204 – 210

7

Helix

213 – 218

6

Beta strand

225 – 228

4

Turn

229 – 232

4

Beta strand

233 – 236

4

Turn

238 – 240

3

Helix

246 – 250

5

Turn

259 – 261

3

Beta strand

263 – 268

6

Beta strand

275 – 277

3

Beta strand

283 – 290

8

Turn

293 – 295

3

Helix

297 – 313

17

Helix

320 – 327

8

Helix

329 – 336

8

Helix

342 – 344

3

Helix

353 – 357

5

Sequences

	Sequence		Length	Mass (Da)
	Q59110-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BA8E4C59216459DA	FASTA	366	41,570
	10 20 30 40 50 60 MVVEGVKTDF GPPYFRDLLH PVIAKNYGKW KYHEVVKPGV IKRVAESGDV IYVVRFGTPR 70 80 90 100 110 120 LLSIYTVREL CDIADKYSDG YLRWTSRNNV EFFVTDESKI DDLINEVQER VGFPCGGTWD 130 140 150 160 170 180 AVKGEYGLSN IVHTQGWIHC HTPAIDASGI VKAVMDELYE YFTDHKLPAM CRISLACCAN 190 200 210 220 230 240 MCGAVHASDI AIVGIHRTPP IPNDEAIRKT CEIPSTVAAC PTGALKPDMK NKTIKVDVEK 250 260 270 280 290 300 CMYCGNCYTM CPGMPLFDPE NDGAAIMVGG KLSEARRMPE LSKVVVPWVP NEPPRWPTLV 310 320 330 340 350 360 KYVKQILEAW AANANKHERL IEWVDRIGWE RFFELTGLEF TQHLIDDYRI TPYFYSEFRA STQFKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dissimilatory sulphite reductase from Archaeoglobus fulgidus: physico-chemical properties of the enzyme and cloning, sequencing and analysis of the reductase genes." Dahl C., Kredich N.M., Deutzmann R., Trueper H.G. J. Gen. Microbiol. 139:1817-1828(1993) [PubMed: 7691984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 332-359. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[2]	"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M95624 Genomic DNA. Translation: AAB17214.1.
AE000782 Genomic DNA. Translation: AAB90811.1. Different initiation.

PIR

H69302.

RefSeq

NP_069260.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C7B	X-ray	2.00	B/E	1-366	[»]

ModBase

Search...

Genome annotation databases

GeneID

1483640.

GenomeReviews

Gene locus AF_0424 in contig AE000782_GR.

KEGG

afu:AF0424.

NMPDR

fig|224325.1.peg.419.

TIGR

AF_0424.

Phylogenomic databases

HOGENOM

Q59110.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_0424-MON.
MetaCyc:MON-12501.

BRENDA

1.8.99.3. 7576.

Family and domain databases

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR011808. DsrB.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S.
[Graphical view]

Pfam

PF00037. Fer4. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02066. dsrB. 1 hit.

PROSITE

PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DSRB_ARCFU

Accession

Primary (citable) accession number: Q59110

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)