Reviewed, UniProtKB/Swiss-Prot Q59106 (ODO1_RALEH)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	H16_A2325

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: InterPro thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 950	950	2-oxoglutarate dehydrogenase E1 component		PRO_0000162160
Experimental info
	Sequence conflict	621	1	G → A in CAA62980. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q59106-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 531BC77561D2ECCF	FASTA	950	105,996
	10 20 30 40 50 60 MMQQYQSNSY LFGGNAPYVE ELYEAYLQNP ASVPDNWRAY FDAMQNVPAV DGSNGRDIPH 70 80 90 100 110 120 APIVASFAER AKQGPIRTIV ASADSDMGRK RVAATQLIAA YRNIGSHWAD LDPLKRQERP 130 140 150 160 170 180 PLPDLDPAFY GFSEADLDIV FNASNTYFGK ESMSLRELLN NLRETYCGTI GFEFMYVSDQ 190 200 210 220 230 240 AQKRWWQERL ETTRSKPVFT LEKKKHILDR LTAAEGLERF LHTKYVGQKR FSLEGGESFI 250 260 270 280 290 300 AAMDELIQHA GSKGVQEIVI GMAHRGRLNV LVNTLGKMPA DLFAEFEGKH VDDLPAGDVK 310 320 330 340 350 360 YHKGFSSDVS TEGGPVHLSL AFNPSHLEIV NPVVEGSAKA RQERRGEVGH KEVLPVQVHG 370 380 390 400 410 420 DAAFAGQGVV METLNLAQTR GYGTGGSMHI VINNQIGFTT SDPRDARSTL YCTDVVKMIE 430 440 450 460 470 480 APVLHVNGDD PEAVVYAMQL AVDFRMEFKK DVVVDIICFR KLGHNEQDTP AVTQPLMYKK 490 500 510 520 530 540 IAQHPGTRKL YADKLAAQNL VPAEFGDEKV KAYRAAMDAG KHTADPVLSN FKNKFAVDWM 550 560 570 580 590 600 PFLNRKWTDA ADTAVPVTEL KRLAERITTT PETLKLHPLV EKVVKDRANM GRGDQPLDWG 610 620 630 640 650 660 MGEHLAFASL VSSGYPVRIT GQDAGRGTFT HRHAVLHDQA RERWDAGSYV PLQNVSENQA 670 680 690 700 710 720 PFTVIDSVLS EEAVLGFEYG YSAAEPNALV IWEAQFGDFV NGAQVVIDQF ISSGEVKWGR 730 740 750 760 770 780 ASGLTLMLPH GYEGQGPEHS SARIERFLQL CADHNMQVCQ PTTPAQIFHL LRRQMIRLFR 790 800 810 820 830 840 KPLVIMTPKS LLRNKDAVSP LSDLAKGHFE TVIPDHEELN ASKVKRVIMC SGKVYYDLVN 850 860 870 880 890 900 TRKEREANDT AVIRLEQLYP FPHKAVAAEL KKYPNATEIV WCQDEPQNQG AWFFVQHYIM 910 920 930 940 950 ENMTDGQKLG YAGRPASASP AVGYYAKHNE QQKALLEAAF AKLKGFVLTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Alcaligenes eutrophus 2-oxoglutarate dehydrogenase complex." Hein S., Steinbuechel A. FEMS Microbiol. Lett. 136:231-238(1996) [PubMed: 8867378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91877 Genomic DNA. Translation: CAA62980.1. AM260479 Genomic DNA. Translation: CAJ93421.1.
PIR	T44422.
RefSeq	YP_726789.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4249723.
GenomeReviews	Gene locus H16_A2325 in contig AM260479_GR.
KEGG	reh:H16_A2325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59106.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DHase_E1. IPR001017. DHase_E1. IPR005475. Transketo_Cen_R. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_RALEH

Accession

Primary (citable) accession number: Q59106
Secondary accession number(s): Q0K9A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)