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Reviewed, UniProtKB/Swiss-Prot Q59105 (NOSZ_RALEH)

Last modified November 25, 2008. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase
Gene names
Name: nosZ
Ordered Locus Names: PHG252
Encoded onPlasmid megaplasmid pHG1
OrganismRalstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

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Protein attributes

Sequence length643 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide.

Catalytic activity

N(2) + H(2)O + acceptor = N(2)O + reduced acceptor.

Cofactor

Binds 2 calcium ions per subunit By similarity.

Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4.

Subunit structure

Homodimer By similarity.

Subcellular location

PeriplasmBy similarity.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Sequence similarities

Belongs to the nosZ family.

In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4646Tat-type signal Potential
Chain47 – 643597Nitrous-oxide reductase
PRO_0000019823

Regions

Region548 – 64396COX2-like

Sites

Metal binding1381Copper Z2 By similarity
Metal binding1391Copper Z3 By similarity
Metal binding1871Copper Z2 By similarity
Metal binding2691Calcium 2; via carbonyl oxygen By similarity
Metal binding2721Calcium 2 By similarity
Metal binding2801Calcium 2; via carbonyl oxygen By similarity
Metal binding2861Calcium 2 By similarity
Metal binding3391Calcium 2 By similarity
Metal binding3411Copper Z1 By similarity
Metal binding3971Copper Z1 By similarity
Metal binding4491Copper Z3 By similarity
Metal binding4701Calcium 1; via carbonyl oxygen By similarity
Metal binding4851Calcium 1 By similarity
Metal binding5101Copper Z4 By similarity
Metal binding5891Copper A1 By similarity
Metal binding6241Copper A1 By similarity
Metal binding6241Copper A2 By similarity
Metal binding6261Copper A2; via carbonyl oxygen By similarity
Metal binding6281Copper A1 By similarity
Metal binding6281Copper A2 By similarity
Metal binding6321Copper A2 By similarity
Metal binding6351Copper A1 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q59105-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 42845B13497E4984

FASTA64370,085
        10         20         30         40         50         60 
MSKEKASIGN GPGGIGRRQF LGTAALAGLA GVVACTDKGA APAAAAVGAP ASGAHGAAHG 

        70         80         90        100        110        120 
AGASVHLKPG ELDTYYGLWS GGHTGDMRVL GLPSGREILR IPCFVPDALV GWGITNESKK 

       130        140        150        160        170        180 
VMGARPDGTL RYTVADTHHT HASYKDGNYD GRYAWVNDKI NSRIARIRLD YFICDKITEL 

       190        200        210        220        230        240 
PNVQGFHGIF PDKRDPVDTK INYTTRVFCG GEFGIPLPSA PTEDAGKYRS LFTCVDAETM 

       250        260        270        280        290        300 
AVRWQVLIDG NCDLVATSYD GKLAATNQYN TENGAHFEDM MSAERDACVF FNIARIEAAV 

       310        320        330        340        350        360 
QAGKFKTYGD SKVPVVDGTQ AANKDPKTAL TAYVSVPKNP HGVNASPDQK YFICAGKLSP 

       370        380        390        400        410        420 
TATVIELSRV LGWFDGKQEK LDDAIVAEVE LGLGPLHTAF DGRGNAYTTL FLDSQLVKWN 

       430        440        450        460        470        480 
LDAAIKFHKG DKNAKYVVDR LDLQYQPGHV NASQSETVAA DGKYLAVGCK FSKDRFLPVG 

       490        500        510        520        530        540 
PLHPENEQLI DISGQKMVLM ADHPVRGEPH DFIIFKRELV RPKQVYALDD FPLAIKDPKE 

       550        560        570        580        590        600 
SGVFRNGRKV TVKITSQAPA FSLREFKLKK GDEVTLILTN LDKIEDLTHG FAIPKYNVNF 

       610        620        630        640 
IVNPQETASV TFVADKPGVF WCYCTHFCHA LHLEMRTRMI VEA

« Hide

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References

« Hide 'large scale' references
[1]"Derived amino acid sequences of the nosZ gene (respiratory N2O reductase) from Alcaligenes eutrophus, Pseudomonas aeruginosa and Pseudomonas stutzeri reveal potential copper-binding residues. Implications for the CuA site of N2O reductase and cytochrome-c oxidase."
Zumft W.G., Dreusch A., Loechelt S., Cuypers H., Friedrich B., Schneider B.
Eur. J. Biochem. 208:31-40(1992) [PubMed: 1324835] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis."
Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G.
J. Mol. Biol. 332:369-383(2003) [PubMed: 12948488] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

X65278 Genomic DNA. Translation: CAA46383.1.
AY305378 Genomic DNA. Translation: AAP86001.1.
PIRS24382.
RefSeqNP_942887.1.

3D structure databases

HSSPHSSP built from PDB template 1FWX based on UniProtKB Q51705.
ModBaseSearch...

Genome annotation databases

GeneID2656479.
GenomeReviewsGene locus PHG252 in contig AY305378_GR.
KEGGreh:PHG252.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ59105.

Family and domain databases

HAMAPMF_00716.
[Tree]
InterProIPR001505. Copper_CuA.
IPR002429. COX2_C.
IPR008972. Cupredoxin.
IPR006311. Tat.
IPR015943. WD40/YVTN_repeat-like.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00116. COX2. 1 hit.
[Graphical view]
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS00078. COX2. False negative.
PS50857. COX2_CUA. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNOSZ_RALEH
AccessionPrimary (citable) accession number: Q59105
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents