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Q59097 (ODP1_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate dehydrogenase E1 component

Short name=PDH E1 component
EC=1.2.4.1
Gene names
Name:pdhA
Ordered Locus Names:H16_A1374
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length895 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2 By similarity.

Catalytic activity

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactor

Thiamine pyrophosphate By similarity.

Subunit structure

Homodimer By similarity. Part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.

Ontologies

Keywords
   Biological processGlycolysis
   LigandPyruvate
Thiamine pyrophosphate
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionpyruvate dehydrogenase (acetyl-transferring) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 895895Pyruvate dehydrogenase E1 component
PRO_0000162238

Sequences

Sequence LengthMass (Da)Tools
Q59097 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 522A9A3C43454051

FASTA895100,365
        10         20         30         40         50         60 
MSAVPEQILG ASSANDADPQ ETHEWLDALQ GVLAAEGPAR AAFLIDKQIE YARVNGVTQP 

        70         80         90        100        110        120 
FHAETQYINT IPVEQQARIP GDQDIEHRIR SYTRWNAMAM VLRANKHTNV GGHISSFASA 

       130        140        150        160        170        180 
ATLYDVGYNH FWRAPSEAGG GDLVFVQGHS APGVYSRAFL LGRLTQDQLD NFRQEVDGKG 

       190        200        210        220        230        240 
ISSYPHPWLM PDFWQFPTVS MGLGPIMAIY QARFMKYLDS RGLAKAGDRK VWAFLGDGET 

       250        260        270        280        290        300 
DEPESLGAIG MAGREKLDNL VFVINCNLQR LDGPVRGNGK IIQELESEFR GAGWNVIKVV 

       310        320        330        340        350        360 
WGSKWDSLLA RDTKGLLMKR MMECVDGEYQ TMKAKDGAYV REHFFNTPEL KAMVADWSDD 

       370        380        390        400        410        420 
DIWRLNRGGH DPHKIYAAYK AASEHKGQPT LILAKTIKGY GMGDAGQAMN VAHQQKKMPV 

       430        440        450        460        470        480 
DAIRKFRDQF NLPVADDQLE EVPYITFPEG SKELEYMRQA RQNLGGYLPA RRQKAEALPV 

       490        500        510        520        530        540 
PQLSAFDALL KATGEGREVS TTMAFVRILN TLLKDKQIGK HVVPIVPDES RTFGMEGLFR 

       550        560        570        580        590        600 
QVGIWNQEGQ KYVPEDHDQL MFYKESQTGQ VLQEGINEAG AMCDWIAAAT SYSTHGVQMI 

       610        620        630        640        650        660 
PFYIYYSMFG IQRIGDLCWA AADMRSRGFL LGGTAGRTTL NGEGLQHEDG HSHVFHAAIP 

       670        680        690        700        710        720 
NCISYDPTFQ YELAVVMQDG LRRMYAEQED VYYYLTVMNE NYEHPEMPAG VEQDIVKGMY 

       730        740        750        760        770        780 
QFRKGVENSN APRVQLLGSG TIFREVIAAA DLLKKDWGVE SDLWGCPSFT ELAREGHDVE 

       790        800        810        820        830        840 
RFNLLHPTET PRESHVAKSL KSARGPVIAS TDYVRAFAEQ IRPFVPRRYV VLGTDGFGRS 

       850        860        870        880        890 
DTREKLRHFF EVDRYWVTLA ALKALADEGA IGRDKVAEAI KKYNLDPNKP NPMSV 

« Hide

References

« Hide 'large scale' references
[1]"Biochemical and molecular characterization of the Alcaligenes eutrophus pyruvate dehydrogenase complex and identification of a new type of dihydrolipoamide dehydrogenase."
Hein S., Steinbuechel A.
J. Bacteriol. 176:4394-4408(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09865 Genomic DNA. Translation: AAA21598.1.
AM260479 Genomic DNA. Translation: CAJ92510.1.
PIRA55514.
RefSeqYP_725878.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ59097.
SMRQ59097. Positions 65-892.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A1374.

Proteomic databases

PRIDEQ59097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ92510; CAJ92510; H16_A1374.
GeneID4250135.
KEGGreh:H16_A1374.
PATRIC35232211. VBIRalEut6770_1763.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2609.
HOGENOMHOG000115215.
KOK00163.
OMAANMIPNC.
OrthoDBEOG6BW4TW.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-1371-MONOMER.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
InterProIPR004660. 2-oxoA_DH_E1.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005474. Transketolase_N.
[Graphical view]
PfamPF00456. Transketolase_N. 2 hits.
[Graphical view]
PIRSFPIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAMSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
TIGRFAMsTIGR00759. aceE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameODP1_CUPNH
AccessionPrimary (citable) accession number: Q59097
Secondary accession number(s): Q0KBW1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program