Q59097 (ODP1_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate dehydrogenase E1 component
Short name=PDH E1 component
EC=1.2.4.1

Gene names

Name:	pdhA
Ordered Locus Names:	H16_A1374

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	895 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2 By similarity.
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity. Makes part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 895	895	Pyruvate dehydrogenase E1 component		PRO_0000162238

Sequences

	Sequence		Length	Mass (Da)
	Q59097 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 522A9A3C43454051	FASTA	895	100,365
	10 20 30 40 50 60 MSAVPEQILG ASSANDADPQ ETHEWLDALQ GVLAAEGPAR AAFLIDKQIE YARVNGVTQP 70 80 90 100 110 120 FHAETQYINT IPVEQQARIP GDQDIEHRIR SYTRWNAMAM VLRANKHTNV GGHISSFASA 130 140 150 160 170 180 ATLYDVGYNH FWRAPSEAGG GDLVFVQGHS APGVYSRAFL LGRLTQDQLD NFRQEVDGKG 190 200 210 220 230 240 ISSYPHPWLM PDFWQFPTVS MGLGPIMAIY QARFMKYLDS RGLAKAGDRK VWAFLGDGET 250 260 270 280 290 300 DEPESLGAIG MAGREKLDNL VFVINCNLQR LDGPVRGNGK IIQELESEFR GAGWNVIKVV 310 320 330 340 350 360 WGSKWDSLLA RDTKGLLMKR MMECVDGEYQ TMKAKDGAYV REHFFNTPEL KAMVADWSDD 370 380 390 400 410 420 DIWRLNRGGH DPHKIYAAYK AASEHKGQPT LILAKTIKGY GMGDAGQAMN VAHQQKKMPV 430 440 450 460 470 480 DAIRKFRDQF NLPVADDQLE EVPYITFPEG SKELEYMRQA RQNLGGYLPA RRQKAEALPV 490 500 510 520 530 540 PQLSAFDALL KATGEGREVS TTMAFVRILN TLLKDKQIGK HVVPIVPDES RTFGMEGLFR 550 560 570 580 590 600 QVGIWNQEGQ KYVPEDHDQL MFYKESQTGQ VLQEGINEAG AMCDWIAAAT SYSTHGVQMI 610 620 630 640 650 660 PFYIYYSMFG IQRIGDLCWA AADMRSRGFL LGGTAGRTTL NGEGLQHEDG HSHVFHAAIP 670 680 690 700 710 720 NCISYDPTFQ YELAVVMQDG LRRMYAEQED VYYYLTVMNE NYEHPEMPAG VEQDIVKGMY 730 740 750 760 770 780 QFRKGVENSN APRVQLLGSG TIFREVIAAA DLLKKDWGVE SDLWGCPSFT ELAREGHDVE 790 800 810 820 830 840 RFNLLHPTET PRESHVAKSL KSARGPVIAS TDYVRAFAEQ IRPFVPRRYV VLGTDGFGRS 850 860 870 880 890 DTREKLRHFF EVDRYWVTLA ALKALADEGA IGRDKVAEAI KKYNLDPNKP NPMSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical and molecular characterization of the Alcaligenes eutrophus pyruvate dehydrogenase complex and identification of a new type of dihydrolipoamide dehydrogenase." Hein S., Steinbuechel A. J. Bacteriol. 176:4394-4408(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09865 Genomic DNA. Translation: AAA21598.1. AM260479 Genomic DNA. Translation: CAJ92510.1.
PIR	A55514.
RefSeq	YP_725878.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q59097.
SMR	Q59097. Positions 65-892.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_A1374.
Proteomic databases
PRIDE	Q59097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ92510; CAJ92510; H16_A1374.
GeneID	4250135.
KEGG	reh:H16_A1374.
PATRIC	35232211. VBIRalEut6770_1763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2609.
HOGENOM	HOG000115215.
KO	K00163.
OMA	AHNTKKV.
ProtClustDB	PRK09405.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-1371-MONOMER.
Family and domain databases
Gene3D	3.40.50.920. 1 hit.
InterPro	IPR004660. 2-oxoA_DH_E1. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005474. Transketolase_N. [Graphical view]
Pfam	PF00456. Transketolase_N. 2 hits. [Graphical view]
PIRSF	PIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00759. aceE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODP1_CUPNH

Accession

Primary (citable) accession number: Q59097
Secondary accession number(s): Q0KBW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program