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Protein

3-carboxy-cis,cis-muconate cycloisomerase

Gene

pcaB

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an anti cycloisomerization.

Catalytic activityi

2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-carboxy-cis,cis-muconate cycloisomerase (pcaB)
  2. 4-carboxymuconolactone decarboxylase (pcaC)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BioCyciASP62977:GJVV-1619-MONOMER.
UniPathwayiUPA00157; UER00265.

Names & Taxonomyi

Protein namesi
Recommended name:
3-carboxy-cis,cis-muconate cycloisomerase (EC:5.5.1.2)
Alternative name(s):
3-carboxymuconate lactonizing enzyme
Short name:
CMLE
Gene namesi
Name:pcaB
Ordered Locus Names:ACIAD1707
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
Proteomesi
  • UP000000430 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4514513-carboxy-cis,cis-muconate cycloisomerasePRO_0000161347Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi62977.ACIAD1707.

Structurei

Secondary structure

1
451
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 94Combined sources
Helixi12 – 176Combined sources
Helixi20 – 4021Combined sources
Helixi46 – 5611Combined sources
Helixi59 – 624Combined sources
Helixi65 – 7511Combined sources
Beta strandi76 – 783Combined sources
Helixi79 – 9113Combined sources
Helixi97 – 1004Combined sources
Turni101 – 1044Combined sources
Helixi107 – 14337Combined sources
Turni144 – 1463Combined sources
Beta strandi148 – 1536Combined sources
Beta strandi156 – 1627Combined sources
Helixi163 – 18826Combined sources
Helixi201 – 2033Combined sources
Helixi207 – 21812Combined sources
Helixi232 – 25827Combined sources
Turni261 – 2633Combined sources
Helixi287 – 30822Combined sources
Helixi320 – 34829Combined sources
Helixi353 – 3619Combined sources
Turni362 – 3654Combined sources
Helixi366 – 3683Combined sources
Helixi369 – 38012Combined sources
Helixi382 – 39918Combined sources
Helixi403 – 4075Combined sources
Helixi411 – 4144Combined sources
Helixi419 – 4257Combined sources
Helixi428 – 4314Combined sources
Helixi435 – 44410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q5NX-ray2.30A1-451[»]
ProteinModelPortaliQ59092.
SMRiQ59092. Positions 3-446.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59092.

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family.Curated

Phylogenomic databases

eggNOGiENOG4107RAW. Bacteria.
COG0015. LUCA.
HOGENOMiHOG000033914.
KOiK01857.
OMAiHRATPMI.
OrthoDBiPOG091H0168.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR012789. Protocat_PcaB.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR02426. protocat_pcaB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59092-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLYASLFY QRDVTEIFSD RALVSYMVEA EVALAQAQAQ VGVIPQSAAT
60 70 80 90 100
VIERAAKTAI DKIDFDALAT ATGLAGNIAI PFVKQLTAIV KDADEDAARY
110 120 130 140 150
VHWGATSQDI LDTACILQCR DALAIVQNQV QQCYETALSQ AQTYRHQVMM
160 170 180 190 200
GRTWLQQALP ITLGHKLARW ASAFKRDLDR INAIKARVLV AQLGGAVGSL
210 220 230 240 250
ASLQDQGSIV VEAYAKQLKL GQTACTWHGE RDRIVEIASV LGIITGNVGK
260 270 280 290 300
MARDWSLMMQ TEIAEVFEPT AKGRGGSSTM PHKRNPVAAA SVLAAANRVP
310 320 330 340 350
ALMSSIYQSM VQEHERSLGA WHAEWLSLPE IFQLTAGALE RTLDVLKGME
360 370 380 390 400
VNAENMHQNI ECTHGLIMAE AVMMALAPHM GRLNAHHVVE AACKTAVAEQ
410 420 430 440 450
KHLKDIISQV DEVKQYFNPS QLDEIFKPES YLGNIQDQID AVLQEAKGEA

K
Length:451
Mass (Da):49,316
Last modified:August 31, 2004 - v2
Checksum:i831FE94AC6574617
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371A → Q in AAC37149 (PubMed:8063101).Curated
Sequence conflicti50 – 567TVIERAA → NCDRTCQQ in AAC37149 (PubMed:8063101).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37149.1.
CR543861 Genomic DNA. Translation: CAG68549.1.
RefSeqiWP_004926621.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG68549; CAG68549; ACIAD1707.
GeneIDi25684309.
KEGGiaci:ACIAD1707.
PATRICi20741184. VBIAciSp98416_1538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37149.1.
CR543861 Genomic DNA. Translation: CAG68549.1.
RefSeqiWP_004926621.1. NC_005966.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q5NX-ray2.30A1-451[»]
ProteinModelPortaliQ59092.
SMRiQ59092. Positions 3-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi62977.ACIAD1707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG68549; CAG68549; ACIAD1707.
GeneIDi25684309.
KEGGiaci:ACIAD1707.
PATRICi20741184. VBIAciSp98416_1538.

Phylogenomic databases

eggNOGiENOG4107RAW. Bacteria.
COG0015. LUCA.
HOGENOMiHOG000033914.
KOiK01857.
OMAiHRATPMI.
OrthoDBiPOG091H0168.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00265.
BioCyciASP62977:GJVV-1619-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ59092.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR012789. Protocat_PcaB.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR02426. protocat_pcaB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCAB_ACIAD
AccessioniPrimary (citable) accession number: Q59092
Secondary accession number(s): Q6FBL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 31, 2004
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.