Reviewed, UniProtKB/Swiss-Prot Q59046 (TOP1_METJA)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	MJ1652

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	DNA topoisomerase 1		PRO_0000145178
Regions
	Zinc finger	600 – 626	27	C4-type 1
	Zinc finger	680 – 706	27	C4-type 2
	Zinc finger	721 – 747	27	C4-type 3
Sites
	Active site	315	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59046-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2F9C95753E202D82	FASTA	761	87,833
	10 20 30 40 50 60 MAEIMTALII CEKPSVAKKI ANALGKAKKK SIDGVPYYEL ERDGKKIIVA SAVGHLFTLV 70 80 90 100 110 120 EKENKEFGFY PVFDIKWVPA SVDKGKEYVN KYIKALKKLS KDADEFYIAT DWDIEGELIG 130 140 150 160 170 180 YHALKYCCGR EKAKRMRFSS LTKKEIVRAF ENPDEIDYGL VDAGESRHIL DWYFGINLSR 190 200 210 220 230 240 ALMNAIRAVN RWKTMSVGRV QGPALAFLTE RELEIKKFIP KPYWVIEALL KDNLKAIHEK 250 260 270 280 290 300 EKFWNEKEAK NVYEKIKDEK SAKVVEIKKT KRKLKPLPPF DLGTLQREAY SYFKISPKET 310 320 330 340 350 360 QEIAQKLYEN ALISYPRTSS QKLPKDRKYL EDILNIIKNH PVYGKWAERI LKENLKPVEG 370 380 390 400 410 420 KKEDPAHPAI HIVDIPKEEL SEKEKEIYDL IARRTLAAFW DNAEREYLNV KIDIKGEKFK 430 440 450 460 470 480 LSGSRTVKEG WHEIYYFPKF DEIELPPLKK NDIIKVEKIT ITRKETQPPK RYTVASIIKE 490 500 510 520 530 540 LEKRGLGTKA TRAEIIDKLI KRGYVIDDGS LKVTDLGISV IETLKRFCPE IIDEKMTRDL 550 560 570 580 590 600 EEKLEKIQFR KIKKDDVLDE AEKRLRKILE EFKKKEEDIG IYLIKNLDAT NKKAKIVGKC 610 620 630 640 650 660 PKCGGDLILI RHKKGRFVGC SNYPECDVKY SLPDKGRIKI PNKVCDACKS PILKIGDREI 670 680 690 700 710 720 CINPECPLKQ VEVKEEDRIC PKCGAKLILK KGVYGAFYGC SNYPKCKYTE PINKKEVVGK 730 740 750 760 CPKCGGDLVV REGKFGKFVG CSNYPKCRYT EKLKLNEKEE K

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99673.1.
PIR	B64506.
RefSeq	NP_248662.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	1452561.
GenomeReviews	Gene locus MJ1652 in contig L77117_GR.
KEGG	mja:MJ1652.
NMPDR	fig|243232.1.peg.1699.
TIGR	MJ1652.
Phylogenomic databases
HOGENOM	Q59046.
OMA	LIICEKP.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1652-MON.
BRENDA	5.99.1.2. 256362.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005739. TopoI_arch. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01057. topA_arch. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_METJA

Accession

Primary (citable) accession number: Q59046

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)