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Q59046

- TOP1_METJA

UniProt

Q59046 - TOP1_METJA

Protein

DNA topoisomerase 1

Gene

topA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121Magnesium 1; catalyticUniRule annotation
    Sitei55 – 551Interaction with DNAUniRule annotation
    Metal bindingi111 – 1111Magnesium 1; catalyticUniRule annotation
    Metal bindingi111 – 1111Magnesium 2UniRule annotation
    Metal bindingi113 – 1131Magnesium 2UniRule annotation
    Sitei167 – 1671Interaction with DNAUniRule annotation
    Sitei171 – 1711Interaction with DNAUniRule annotation
    Active sitei315 – 3151O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei317 – 3171Interaction with DNAUniRule annotation
    Sitei502 – 5021Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri600 – 62627C4-type 1Add
    BLAST
    Zinc fingeri680 – 70627C4-type 2Add
    BLAST
    Zinc fingeri721 – 74727C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:MJ1652
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 761761DNA topoisomerase 1PRO_0000145178Add
    BLAST

    Proteomic databases

    PRIDEiQ59046.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243232.MJ1652.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59046.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 143138ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni196 – 2016Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri600 – 62627C4-type 1Add
    BLAST
    Zinc fingeri680 – 70627C4-type 2Add
    BLAST
    Zinc fingeri721 – 74727C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    KOiK03168.
    OMAiWINKAKK.
    PhylomeDBiQ59046.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005739. TopoI_arch.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 3 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01057. topA_arch. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEIMTALII CEKPSVAKKI ANALGKAKKK SIDGVPYYEL ERDGKKIIVA    50
    SAVGHLFTLV EKENKEFGFY PVFDIKWVPA SVDKGKEYVN KYIKALKKLS 100
    KDADEFYIAT DWDIEGELIG YHALKYCCGR EKAKRMRFSS LTKKEIVRAF 150
    ENPDEIDYGL VDAGESRHIL DWYFGINLSR ALMNAIRAVN RWKTMSVGRV 200
    QGPALAFLTE RELEIKKFIP KPYWVIEALL KDNLKAIHEK EKFWNEKEAK 250
    NVYEKIKDEK SAKVVEIKKT KRKLKPLPPF DLGTLQREAY SYFKISPKET 300
    QEIAQKLYEN ALISYPRTSS QKLPKDRKYL EDILNIIKNH PVYGKWAERI 350
    LKENLKPVEG KKEDPAHPAI HIVDIPKEEL SEKEKEIYDL IARRTLAAFW 400
    DNAEREYLNV KIDIKGEKFK LSGSRTVKEG WHEIYYFPKF DEIELPPLKK 450
    NDIIKVEKIT ITRKETQPPK RYTVASIIKE LEKRGLGTKA TRAEIIDKLI 500
    KRGYVIDDGS LKVTDLGISV IETLKRFCPE IIDEKMTRDL EEKLEKIQFR 550
    KIKKDDVLDE AEKRLRKILE EFKKKEEDIG IYLIKNLDAT NKKAKIVGKC 600
    PKCGGDLILI RHKKGRFVGC SNYPECDVKY SLPDKGRIKI PNKVCDACKS 650
    PILKIGDREI CINPECPLKQ VEVKEEDRIC PKCGAKLILK KGVYGAFYGC 700
    SNYPKCKYTE PINKKEVVGK CPKCGGDLVV REGKFGKFVG CSNYPKCRYT 750
    EKLKLNEKEE K 761
    Length:761
    Mass (Da):87,833
    Last modified:November 1, 1996 - v1
    Checksum:i2F9C95753E202D82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99673.1.
    PIRiB64506.
    RefSeqiNP_248662.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99673; AAB99673; MJ_1652.
    GeneIDi1452561.
    KEGGimja:MJ_1652.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99673.1 .
    PIRi B64506.
    RefSeqi NP_248662.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q59046.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1652.

    Proteomic databases

    PRIDEi Q59046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99673 ; AAB99673 ; MJ_1652 .
    GeneIDi 1452561.
    KEGGi mja:MJ_1652.

    Phylogenomic databases

    eggNOGi COG0551.
    KOi K03168.
    OMAi WINKAKK.
    PhylomeDBi Q59046.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005739. TopoI_arch.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 3 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01057. topA_arch. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiTOP1_METJA
    AccessioniPrimary (citable) accession number: Q59046
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3