Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q59046

- TOP1_METJA

UniProt

Q59046 - TOP1_METJA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA topoisomerase 1

Gene

topA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Magnesium 1; catalyticUniRule annotation
Sitei55 – 551Interaction with DNAUniRule annotation
Metal bindingi111 – 1111Magnesium 1; catalyticUniRule annotation
Metal bindingi111 – 1111Magnesium 2UniRule annotation
Metal bindingi113 – 1131Magnesium 2UniRule annotation
Sitei167 – 1671Interaction with DNAUniRule annotation
Sitei171 – 1711Interaction with DNAUniRule annotation
Active sitei315 – 3151O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei317 – 3171Interaction with DNAUniRule annotation
Sitei502 – 5021Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri600 – 62627C4-type 1Add
BLAST
Zinc fingeri680 – 70627C4-type 2Add
BLAST
Zinc fingeri721 – 74727C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MJ1652
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 761761DNA topoisomerase 1PRO_0000145178Add
BLAST

Proteomic databases

PRIDEiQ59046.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ1652.

Structurei

3D structure databases

ProteinModelPortaliQ59046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 143138ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni196 – 2016Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri600 – 62627C4-type 1Add
BLAST
Zinc fingeri680 – 70627C4-type 2Add
BLAST
Zinc fingeri721 – 74727C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
InParanoidiQ59046.
KOiK03168.
OMAiWINKAKK.
PhylomeDBiQ59046.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01057. topA_arch. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59046-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEIMTALII CEKPSVAKKI ANALGKAKKK SIDGVPYYEL ERDGKKIIVA
60 70 80 90 100
SAVGHLFTLV EKENKEFGFY PVFDIKWVPA SVDKGKEYVN KYIKALKKLS
110 120 130 140 150
KDADEFYIAT DWDIEGELIG YHALKYCCGR EKAKRMRFSS LTKKEIVRAF
160 170 180 190 200
ENPDEIDYGL VDAGESRHIL DWYFGINLSR ALMNAIRAVN RWKTMSVGRV
210 220 230 240 250
QGPALAFLTE RELEIKKFIP KPYWVIEALL KDNLKAIHEK EKFWNEKEAK
260 270 280 290 300
NVYEKIKDEK SAKVVEIKKT KRKLKPLPPF DLGTLQREAY SYFKISPKET
310 320 330 340 350
QEIAQKLYEN ALISYPRTSS QKLPKDRKYL EDILNIIKNH PVYGKWAERI
360 370 380 390 400
LKENLKPVEG KKEDPAHPAI HIVDIPKEEL SEKEKEIYDL IARRTLAAFW
410 420 430 440 450
DNAEREYLNV KIDIKGEKFK LSGSRTVKEG WHEIYYFPKF DEIELPPLKK
460 470 480 490 500
NDIIKVEKIT ITRKETQPPK RYTVASIIKE LEKRGLGTKA TRAEIIDKLI
510 520 530 540 550
KRGYVIDDGS LKVTDLGISV IETLKRFCPE IIDEKMTRDL EEKLEKIQFR
560 570 580 590 600
KIKKDDVLDE AEKRLRKILE EFKKKEEDIG IYLIKNLDAT NKKAKIVGKC
610 620 630 640 650
PKCGGDLILI RHKKGRFVGC SNYPECDVKY SLPDKGRIKI PNKVCDACKS
660 670 680 690 700
PILKIGDREI CINPECPLKQ VEVKEEDRIC PKCGAKLILK KGVYGAFYGC
710 720 730 740 750
SNYPKCKYTE PINKKEVVGK CPKCGGDLVV REGKFGKFVG CSNYPKCRYT
760
EKLKLNEKEE K
Length:761
Mass (Da):87,833
Last modified:November 1, 1996 - v1
Checksum:i2F9C95753E202D82
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99673.1.
PIRiB64506.
RefSeqiNP_248662.1. NC_000909.1.
WP_010871176.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99673; AAB99673; MJ_1652.
GeneIDi1452561.
KEGGimja:MJ_1652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99673.1 .
PIRi B64506.
RefSeqi NP_248662.1. NC_000909.1.
WP_010871176.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q59046.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1652.

Proteomic databases

PRIDEi Q59046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99673 ; AAB99673 ; MJ_1652 .
GeneIDi 1452561.
KEGGi mja:MJ_1652.

Phylogenomic databases

eggNOGi COG0551.
InParanoidi Q59046.
KOi K03168.
OMAi WINKAKK.
PhylomeDBi Q59046.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01057. topA_arch. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiTOP1_METJA
AccessioniPrimary (citable) accession number: Q59046
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3