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Protein

tRNA (pseudouridine(54)-N(1))-methyltransferase

Gene

trmY

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.UniRule annotation2 Publications

Catalytic activityi

S-adenosyl-L-methionine + pseudouridine(54) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(54) in tRNA.UniRule annotation2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication
Binding sitei156 – 1561S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1 Publication
Binding sitei189 – 1891S-adenosyl-L-methionineUniRule annotation1 Publication

GO - Molecular functioni

  • S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB
  • tRNA methyltransferase activity Source: UniProtKB

GO - Biological processi

  • tRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.257. 3260.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (pseudouridine(54)-N(1))-methyltransferaseUniRule annotation (EC:2.1.1.257UniRule annotation)
Gene namesi
Name:trmYUniRule annotation
Ordered Locus Names:MJ1640
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205tRNA (pseudouridine(54)-N(1))-methyltransferasePRO_0000157948Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1640.

Structurei

Secondary structure

1
205
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109Combined sources
Helixi19 – 213Combined sources
Turni22 – 276Combined sources
Helixi29 – 4012Combined sources
Beta strandi43 – 464Combined sources
Beta strandi50 – 567Combined sources
Beta strandi59 – 613Combined sources
Beta strandi64 – 696Combined sources
Turni70 – 723Combined sources
Helixi80 – 9617Combined sources
Helixi101 – 1033Combined sources
Beta strandi106 – 1094Combined sources
Beta strandi112 – 1154Combined sources
Helixi119 – 12810Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi140 – 1423Combined sources
Helixi143 – 1453Combined sources
Beta strandi150 – 1567Combined sources
Helixi163 – 1719Combined sources
Beta strandi175 – 1784Combined sources
Helixi186 – 19914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AI9X-ray1.55X1-205[»]
3AIAX-ray1.40A/B1-205[»]
ProteinModelPortaliQ59034.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59034.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 1846S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the methyltransferase superfamily. TrmY family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01239. Archaea.
COG1901. LUCA.
InParanoidiQ59034.
KOiK16317.
OMAiHADHCII.
PhylomeDBiQ59034.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00587. tRNA_methyltr_TrmY. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR007158. TrmY.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04013. Methyltrn_RNA_2. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

Q59034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREFIFKANK TITSSDINLK DLPGSCGRLD LLCRCVSDAF FLSHDIRRDV
60 70 80 90 100
VFYAVLYGQP NPPVCIKFVG SELKKVSPDE RNIAIFIKKA LKKFEELDEE
110 120 130 140 150
QRKDWNQSTP GIYVRRLGFR NLVLEKLEEG KNIYYLHMNG EDVENVDIEN
160 170 180 190 200
PVFIIGDHIG IGEEDERFLD EIKAKRISLS PLELHANHCI TIIHNVLDKK

RICEI
Length:205
Mass (Da):23,704
Last modified:November 1, 1996 - v1
Checksum:iEBED16985B07E132
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99660.1.
PIRiF64504.

Genome annotation databases

EnsemblBacteriaiAAB99660; AAB99660; MJ_1640.
KEGGimja:MJ_1640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99660.1.
PIRiF64504.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AI9X-ray1.55X1-205[»]
3AIAX-ray1.40A/B1-205[»]
ProteinModelPortaliQ59034.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99660; AAB99660; MJ_1640.
KEGGimja:MJ_1640.

Phylogenomic databases

eggNOGiarCOG01239. Archaea.
COG1901. LUCA.
InParanoidiQ59034.
KOiK16317.
OMAiHADHCII.
PhylomeDBiQ59034.

Enzyme and pathway databases

BRENDAi2.1.1.257. 3260.

Miscellaneous databases

EvolutionaryTraceiQ59034.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00587. tRNA_methyltr_TrmY. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR007158. TrmY.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04013. Methyltrn_RNA_2. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMY_METJA
AccessioniPrimary (citable) accession number: Q59034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.