DNA polymerase II large subunit - Methanocaldococcus jannaschii

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Reviewed, UniProtKB/Swiss-Prot Q59024 (DP2L_METJA)

Last modified July 22, 2008. Version 54. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MJ1630

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1139 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
Subunit structure	Heterodimer of a large subunit and a small subunit.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1139

DNA polymerase II large subunit

PRO_0000152575

Sequences

Sequence

Length

Mass (Da)

Tools

Q59024-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3B7968A013AAC45A
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1,139

129,402

        10         20         30         40         50         60 
MIVMVHVACS ENMKKYFENI VDEVKKIYRI AEECRKKGFD PTDEVEIPLA ADMADRVEGL 

        70         80         90        100        110        120 
VGPKGVAERI RELVKELGKE PAALEIAKEI VEGKFGNFDK EKKAEQAVRT ALAVLTEGIV 

       130        140        150        160        170        180 
AAPLEGIADV KIKKNPDGTE YLAIYYAGPI RSAGGTAQAL SVLVGDFVRK AMGLDRYKPT 

       190        200        210        220        230        240 
EDEIERYVEE VELYQSEVGS FQYNPTADEI RTAIRNIPIE ITGEATDDVE VSGHRDLPRV 

       250        260        270        280        290        300 
ETNQLRGGAL LVLVEGVLLK APKILRHVDK LGIEGWDWLK DLMSKKEEKE EEKDEKVDDE 

       310        320        330        340        350        360 
EIDEEEEEIS GYWRDVKIEA NKKFISEVIA GRPVFAHPSK VGGFRLRYGR SRNTGFATQG 

       370        380        390        400        410        420 
FHPALMYLVD EFMAVGTQLK TERPGKATCV VPVDSIEPPI VKLKNGDVIR VDTIEKAMDV 

       430        440        450        460        470        480 
RNRVEEILFL GDVLVNYGDF LENNHPLLPS CWCEEWYEKI LIANNIEYDK DFIKNPKPEE 

       490        500        510        520        530        540 
AVKFALETKT PLHPRFTYHW HDVSKEDIIL LRNWLLKGKE DSLEGKKVWI VDLEIEEDKK 

       550        560        570        580        590        600 
AKRILELIGC CHLVRNKKVI IEEYYPLLYS LGFDVENKKD LVENIEKILE SAKNSMHLIN 

       610        620        630        640        650        660 
LLAPFEVRRN TYVYVGARMG RPEKAAPRKM KPPVNGLFPI GNAGGQVRLI NKAVEENNTD 

       670        680        690        700        710        720 
DVDVSYTRCP NCGKISLYRV CPFCGTKVEL DNFGRIKAPL KDYWYAALKR LGINKPGDVK 

       730        740        750        760        770        780 
CIKGMTSKQK IVEPLEKAIL RAINEVYVFK DGTTRFDCTD VPVTHFKPNE INVTVEKLRE 

       790        800        810        820        830        840 
LGYDKDIYGN ELVDGEQVVE LKPQDVIIPE SCAEYFVKVA NFIDDLLEKF YKVERFYNVK 

       850        860        870        880        890        900 
KKEDLIGHLV IGMAPHTSAG MVGRIIGYTK ANVGYAHPYF HAAKRRNCDG DEDSFFLLLD 

       910        920        930        940        950        960 
AFLNFSKKFL PDKRGGQMDA PLVLTTILDP KEVDGEVHNM DTMWSYPLEF YEKTLEMPSP 

       970        980        990       1000       1010       1020 
KEVKEFMETV EDRLGKPEQY EGIGYTHETS RIDLGPKVCA YKTLGSMLEK TTSQLSVAKK 

      1030       1040       1050       1060       1070       1080 
IRATDERDVA EKVIQSHFIP DLIGNLRAFS RQAVRCKCGA KYRRIPLKGK CPKCGSNLIL 

      1090       1100       1110       1120       1130 
TVSKGAVEKY MDVAEKMAEE YNVNDYIKQR LKIIKEGINS IFENEKSRQV KLSDFFKIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"A novel DNA polymerase family found in Archaea." Ishino Y., Komori K., Cann I.K.O., Koga Y. J. Bacteriol. 180:2232-2236(1998) [PubMed: 9555910] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
	L77117 Genomic DNA. Translation: AAB99651.1.
PIR	D64503.
RefSeq	NP_248640.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1452539.
GenomeReviews	Gene locus MJ1630 in contig L77117_GR.
KEGG	mja:MJ1630.
NMPDR	fig\|243232.1.peg.1677.
TIGR	MJ1630.
Phylogenomic databases
HOGENOM	Q59024.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1630-MON.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METJA

Accession

Primary (citable) accession number: Q59024

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents