Reviewed, UniProtKB/Swiss-Prot Q59014 (BPL_METJA)

Names and origin

Protein names

Recommended name:
    Putative biotin ligase
    EC=6.3.4.15

Gene names

Ordered Locus Names:

MJ1619

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].
Sequence similarities	Belongs to the biotin--protein ligase family.

Ontologies

Keywords
Ligand	ATP-binding Biotin Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW biotin-[acetyl-CoA-carboxylase] ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 237

237

Putative biotin ligase

PRO_0000064982

Secondary structure

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237

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

12 – 21

10

Beta strand

26 – 32

7

Beta strand

34 – 36

3

Helix

40 – 42

3

Beta strand

51 – 59

9

Helix

65 – 80

16

Turn

81 – 83

3

Beta strand

88 – 91

4

Turn

92 – 94

3

Beta strand

95 – 100

6

Beta strand

103 – 114

12

Beta strand

116 – 125

10

Beta strand

127 – 129

3

Helix

133 – 137

5

Helix

142 – 146

5

Helix

152 – 171

20

Helix

177 – 187

11

Beta strand

188 – 190

3

Beta strand

194 – 199

6

Beta strand

204 – 212

9

Beta strand

214 – 221

8

Beta strand

224 – 229

6

Helix

230 – 232

3

Beta strand

233 – 236

4

Sequences

	Sequence		Length	Mass (Da)
	Q59014-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 602506218437B1F6	FASTA	237	27,185
	10 20 30 40 50 60 MEIIHLSEID STNDYAKELA KEGKRNFIVL ADKQNNGKGR WGRVWYSDEG GLYFSMVLDS 70 80 90 100 110 120 KLYNPKVINL LVPICIIEVL KNYVDKELGL KFPNDIMVKV NDNYKKLGGI LTELTDDYMI 130 140 150 160 170 180 IGIGINVNNQ IRNEIREIAI SLKEITGKEL DKVEILSNFL KTFESYLEKL KNKEIDDYEI 190 200 210 220 230 LKKYKKYSIT IGKQVKILLS NNEIITGKVY DIDFDGIVLG TEKGIERIPS GICIHVR

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L77117 Genomic DNA. Translation: AAB99640.1.

PIR

B64502.

RefSeq

NP_248629.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EJ9	X-ray	2.00	A	1-237	[»]

ModBase

Search...

Genome annotation databases

GeneID

1452528.

GenomeReviews

Gene locus MJ1619 in contig L77117_GR.

KEGG

mja:MJ1619.

NMPDR

fig|243232.1.peg.1666.

TIGR

MJ1619.

Phylogenomic databases

HOGENOM

Q59014.

OMA

GLNINES.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_1619-MON.

BRENDA

6.3.4.15. 256362.

Family and domain databases

InterPro

IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LipA_LipB.
[Graphical view]

PANTHER

PTHR12835. BirA_ligase. 1 hit.

Pfam

PF02237. BPL_C. 1 hit.
PF03099. BPL_LipA_LipB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00121. birA_ligase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

BPL_METJA

Accession

Primary (citable) accession number: Q59014

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)