Q59000 (G6PI_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate isomerase
Short name=GPI
EC=5.3.1.9

Alternative name(s):
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI

Gene names

Name:	pgi
Ordered Locus Names:	MJ1605

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the isomerization of glucose-6-P to fructose-6-P. Ref.2 Ref.3
Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_00473
Enzyme regulation	Competively inhibited by 6-phosphogluconate and erythrose 4-phosphate. Ref.2
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_00473
Subunit structure	Homodimer. Ref.2
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GPI family.
Biophysicochemical properties	Kinetic parameters: KM=0.04 mM for F6P (at pH 6.3 and 50 degrees Celsius) Ref.2 KM=1 mM for G6P (at pH 6.3 and 50 degrees Celsius) Vmax=9 µmol/min/mg enzyme for G6P (at pH 6.3 and 50 degrees Celsius) Vmax=21 µmol/min/mg enzyme for F6P (at pH 6.3 and 50 degrees Celsius) pH dependence: Optimum pH is 6.3. 50% remaining activity is observed at pH 5.3 and pH 7. Temperature dependence: Optimum temperature is 89 degrees Celsius. It does not lose activity upon incubation at 80 degrees Celsius for about 120 min and still has a half-life at 95 degrees Celsius of 40 min. At 100 degrees Celsius, an almost complete loss of activity is observed after 30 min.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucose-6-phosphate isomerase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Glucose-6-phosphate isomerase HAMAP-Rule MF_00473		PRO_0000180780
Sites
	Active site	261	1	Proton donor By similarity
	Active site	282	1	By similarity
	Active site	392	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59000 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D5B766A9F2230ED9	FASTA	401	45,675
	10 20 30 40 50 60 MLSYDYENAL KVGEISLEDI NKVDFANAYS NLMEKLDNGV VGFRDVIYDE NLDKYKSLNG 70 80 90 100 110 120 YENVVVIGMG GSILGTMAIY YAISPFNNNA YFIDNSDPEK TLSILKKVDL NESIIYIISK 130 140 150 160 170 180 SGNTLETLVN YYLIKKRIEK LNSFKGKLVF ITNGGKLKRE AEKNNYDIFS IPENVPGRFS 190 200 210 220 230 240 VFTAVGLAPL YSLGVDISKI LEGAREMDKI CQNEDILKNP ALLNGVIHYL YDKRGKDISV 250 260 270 280 290 300 IMSYVESLKY FGDWYKQLIG ESLGKNKHGI TPLLSIGAKD QHSLLQLYMD GKKDKIITFM 310 320 330 340 350 360 VAKKYRLDEE IEFEDINDEK ISCRYSDIIR SQQKATEIAL TNNGVPNVRI TLDEINEMAM 370 380 390 400 GALLYMYEMQ VGFMGELYNI NAYNQPAVEE EKKICWRLIK Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily." Rudolph B., Hansen T., Schonheit P. Arch. Microbiol. 181:82-87(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
[3]	"Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii." White R.H., Xu H. Biochemistry 45:12366-12379(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99624.1.
PIR	D64500.
RefSeq	NP_248615.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q59000.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ1605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB99624; AAB99624; MJ_1605.
GeneID	1452514.
KEGG	mja:MJ_1605.
Phylogenomic databases
eggNOG	COG0166.
KO	K01810.
OMA	QLQLYMD.
ProtClustDB	PRK00973.
Enzyme and pathway databases
SABIO-RK	Q59000.
UniPathway	UPA00109; UER00181.
Family and domain databases
HAMAP	MF_00473. G6P_isomerase.
InterPro	IPR001672. G6P_Isomerase. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
PANTHER	PTHR11469. PTHR11469. 1 hit.
Pfam	PF00342. PGI. 1 hit. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PI_METJA

Accession

Primary (citable) accession number: Q59000

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program