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Q59000

- G6PI_METJA

UniProt

Q59000 - G6PI_METJA

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Protein

Glucose-6-phosphate isomerase

Gene

pgi

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of glucose-6-P to fructose-6-P.2 Publications

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Competively inhibited by 6-phosphogluconate and erythrose 4-phosphate.1 Publication

Kineticsi

  1. KM=0.04 mM for F6P (at pH 6.3 and 50 degrees Celsius)1 Publication
  2. KM=1 mM for G6P (at pH 6.3 and 50 degrees Celsius)1 Publication

Vmax=9 µmol/min/mg enzyme for G6P (at pH 6.3 and 50 degrees Celsius)1 Publication

Vmax=21 µmol/min/mg enzyme for F6P (at pH 6.3 and 50 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 6.3. 50% remaining activity is observed at pH 5.3 and pH 7.1 Publication

Temperature dependencei

Optimum temperature is 89 degrees Celsius. It does not lose activity upon incubation at 80 degrees Celsius for about 120 min and still has a half-life at 95 degrees Celsius of 40 min. At 100 degrees Celsius, an almost complete loss of activity is observed after 30 min.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei261 – 2611Proton donorBy similarity
Active sitei282 – 2821By similarity
Active sitei392 – 3921By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: UniProtKB

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

SABIO-RKQ59000.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgi
Ordered Locus Names:MJ1605
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Glucose-6-phosphate isomerasePRO_0000180780Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi243232.MJ1605.

Structurei

3D structure databases

ProteinModelPortaliQ59000.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiCOG0166.
InParanoidiQ59000.
KOiK01810.
OMAiAIAFREF.
PhylomeDBiQ59000.

Family and domain databases

HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q59000-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSYDYENAL KVGEISLEDI NKVDFANAYS NLMEKLDNGV VGFRDVIYDE
60 70 80 90 100
NLDKYKSLNG YENVVVIGMG GSILGTMAIY YAISPFNNNA YFIDNSDPEK
110 120 130 140 150
TLSILKKVDL NESIIYIISK SGNTLETLVN YYLIKKRIEK LNSFKGKLVF
160 170 180 190 200
ITNGGKLKRE AEKNNYDIFS IPENVPGRFS VFTAVGLAPL YSLGVDISKI
210 220 230 240 250
LEGAREMDKI CQNEDILKNP ALLNGVIHYL YDKRGKDISV IMSYVESLKY
260 270 280 290 300
FGDWYKQLIG ESLGKNKHGI TPLLSIGAKD QHSLLQLYMD GKKDKIITFM
310 320 330 340 350
VAKKYRLDEE IEFEDINDEK ISCRYSDIIR SQQKATEIAL TNNGVPNVRI
360 370 380 390 400
TLDEINEMAM GALLYMYEMQ VGFMGELYNI NAYNQPAVEE EKKICWRLIK

Q
Length:401
Mass (Da):45,675
Last modified:November 1, 1996 - v1
Checksum:iD5B766A9F2230ED9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99624.1.
PIRiD64500.
RefSeqiNP_248615.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99624; AAB99624; MJ_1605.
GeneIDi1452514.
KEGGimja:MJ_1605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99624.1 .
PIRi D64500.
RefSeqi NP_248615.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q59000.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99624 ; AAB99624 ; MJ_1605 .
GeneIDi 1452514.
KEGGi mja:MJ_1605.

Phylogenomic databases

eggNOGi COG0166.
InParanoidi Q59000.
KOi K01810.
OMAi AIAFREF.
PhylomeDBi Q59000.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00181 .
SABIO-RK Q59000.

Family and domain databases

HAMAPi MF_00473. G6P_isomerase.
InterProi IPR001672. G6P_Isomerase.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view ]
PANTHERi PTHR11469. PTHR11469. 1 hit.
Pfami PF00342. PGI. 1 hit.
[Graphical view ]
PRINTSi PR00662. G6PISOMERASE.
PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily."
    Rudolph B., Hansen T., Schonheit P.
    Arch. Microbiol. 181:82-87(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
  3. "Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii."
    White R.H., Xu H.
    Biochemistry 45:12366-12379(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE.

Entry informationi

Entry nameiG6PI_METJA
AccessioniPrimary (citable) accession number: Q59000
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3