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Q59000

- G6PI_METJA

UniProt

Q59000 - G6PI_METJA

Protein

Glucose-6-phosphate isomerase

Gene

pgi

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization of glucose-6-P to fructose-6-P.2 Publications

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.

    Enzyme regulationi

    Competively inhibited by 6-phosphogluconate and erythrose 4-phosphate.1 Publication

    Kineticsi

    1. KM=0.04 mM for F6P (at pH 6.3 and 50 degrees Celsius)1 Publication
    2. KM=1 mM for G6P (at pH 6.3 and 50 degrees Celsius)1 Publication

    Vmax=9 µmol/min/mg enzyme for G6P (at pH 6.3 and 50 degrees Celsius)1 Publication

    Vmax=21 µmol/min/mg enzyme for F6P (at pH 6.3 and 50 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.3. 50% remaining activity is observed at pH 5.3 and pH 7.1 Publication

    Temperature dependencei

    Optimum temperature is 89 degrees Celsius. It does not lose activity upon incubation at 80 degrees Celsius for about 120 min and still has a half-life at 95 degrees Celsius of 40 min. At 100 degrees Celsius, an almost complete loss of activity is observed after 30 min.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei261 – 2611Proton donorBy similarity
    Active sitei282 – 2821By similarity
    Active sitei392 – 3921By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate isomerase activity Source: UniProtKB

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Gluconeogenesis, Glycolysis

    Enzyme and pathway databases

    SABIO-RKQ59000.
    UniPathwayiUPA00109; UER00181.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate isomerase (EC:5.3.1.9)
    Short name:
    GPI
    Alternative name(s):
    Phosphoglucose isomerase
    Short name:
    PGI
    Phosphohexose isomerase
    Short name:
    PHI
    Gene namesi
    Name:pgi
    Ordered Locus Names:MJ1605
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 401401Glucose-6-phosphate isomerasePRO_0000180780Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ1605.

    Structurei

    3D structure databases

    ProteinModelPortaliQ59000.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI family.Curated

    Phylogenomic databases

    eggNOGiCOG0166.
    KOiK01810.
    OMAiAIAFREF.
    PhylomeDBiQ59000.

    Family and domain databases

    HAMAPiMF_00473. G6P_isomerase.
    InterProiIPR001672. G6P_Isomerase.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view]
    PANTHERiPTHR11469. PTHR11469. 1 hit.
    PfamiPF00342. PGI. 1 hit.
    [Graphical view]
    PRINTSiPR00662. G6PISOMERASE.
    PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q59000-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSYDYENAL KVGEISLEDI NKVDFANAYS NLMEKLDNGV VGFRDVIYDE    50
    NLDKYKSLNG YENVVVIGMG GSILGTMAIY YAISPFNNNA YFIDNSDPEK 100
    TLSILKKVDL NESIIYIISK SGNTLETLVN YYLIKKRIEK LNSFKGKLVF 150
    ITNGGKLKRE AEKNNYDIFS IPENVPGRFS VFTAVGLAPL YSLGVDISKI 200
    LEGAREMDKI CQNEDILKNP ALLNGVIHYL YDKRGKDISV IMSYVESLKY 250
    FGDWYKQLIG ESLGKNKHGI TPLLSIGAKD QHSLLQLYMD GKKDKIITFM 300
    VAKKYRLDEE IEFEDINDEK ISCRYSDIIR SQQKATEIAL TNNGVPNVRI 350
    TLDEINEMAM GALLYMYEMQ VGFMGELYNI NAYNQPAVEE EKKICWRLIK 400
    Q 401
    Length:401
    Mass (Da):45,675
    Last modified:November 1, 1996 - v1
    Checksum:iD5B766A9F2230ED9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99624.1.
    PIRiD64500.
    RefSeqiNP_248615.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99624; AAB99624; MJ_1605.
    GeneIDi1452514.
    KEGGimja:MJ_1605.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99624.1 .
    PIRi D64500.
    RefSeqi NP_248615.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q59000.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99624 ; AAB99624 ; MJ_1605 .
    GeneIDi 1452514.
    KEGGi mja:MJ_1605.

    Phylogenomic databases

    eggNOGi COG0166.
    KOi K01810.
    OMAi AIAFREF.
    PhylomeDBi Q59000.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00181 .
    SABIO-RK Q59000.

    Family and domain databases

    HAMAPi MF_00473. G6P_isomerase.
    InterProi IPR001672. G6P_Isomerase.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view ]
    PANTHERi PTHR11469. PTHR11469. 1 hit.
    Pfami PF00342. PGI. 1 hit.
    [Graphical view ]
    PRINTSi PR00662. G6PISOMERASE.
    PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily."
      Rudolph B., Hansen T., Schonheit P.
      Arch. Microbiol. 181:82-87(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
    3. "Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii."
      White R.H., Xu H.
      Biochemistry 45:12366-12379(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE.

    Entry informationi

    Entry nameiG6PI_METJA
    AccessioniPrimary (citable) accession number: Q59000
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3