Q58HT5 (AWAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-CoA wax alcohol acyltransferase 1
EC=2.3.1.75

Alternative name(s):
Diacylglycerol O-acyltransferase 2-like protein 3
Diacylglycerol acyltransferase 2
Long-chain-alcohol O-fatty-acyltransferase 1

Gene names

Name:	AWAT1
Synonyms:	DGA2, DGAT2L3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference for arachidyl alcohol as well as decyl alcohol, demonstrating its relatively poor activity using saturated long chain alcohols (C16, C18, and C20). Ref.1
Catalytic activity	Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis. Ref.1
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.
Biophysicochemical properties	Kinetic parameters: Vmax=31.7 pmol/min/mg enzyme with [14C]oleoyl-CoA as substrate Ref.1 Vmax=113 pmol/min/mg enzyme with [14C]cetyl-CoA as substrate

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	long-chain-alcohol O-fatty-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Acyl-CoA wax alcohol acyltransferase 1		PRO_0000249051
Regions
	Transmembrane	12 – 32	21	Helical; Potential
	Transmembrane	34 – 53	20	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58HT5 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 51475CFD31667215	FASTA	328	37,759
	10 20 30 40 50 60 MAHSKQPSHF QSLMLLQWPL SYLAIFWILQ PLFVYLLFTS LWPLPVLYFA WLFLDWKTPE 70 80 90 100 110 120 RGGRRSAWVR NWCVWTHIRD YFPITILKTK DLSPEHNYLM GVHPHGLLTF GAFCNFCTEA 130 140 150 160 170 180 TGFSKTFPGI TPHLATLSWF FKIPFVREYL MAKGVCSVSQ PAINYLLSHG TGNLVGIVVG 190 200 210 220 230 240 GVGEALQSVP NTTTLILQKR KGFVRTALQH GAHLVPTFTF GETEVYDQVL FHKDSRMYKF 250 260 270 280 290 300 QSCFRRIFGF YCCVFYGQSF CQGSTGLLPY SRPIVTVVGE PLPLPQIEKP SQEMVDKYHA 310 320 LYMDALHKLF DQHKTHYGCS ETQKLFFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two novel human Acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily." Turkish A.R., Henneberry A.L., Cromley D., Padamsee M., Oelkers P., Bazzi H., Christiano A.M., Billheimer J.T., Sturley S.L. J. Biol. Chem. 280:14755-14764(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-328. Tissue: Skin.
[4]	"Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals." Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R. Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY947638 mRNA. Translation: AAX48018.1. AL357752 Genomic DNA. Translation: CAI41493.2. BC039181 Genomic DNA. No translation available. BN000155 mRNA. Translation: CAD89266.1.
IPI	IPI00419338.
RefSeq	NP_001013597.1. NM_001013579.2.
UniGene	Hs.407641.
3D structure databases
ProteinModelPortal	Q58HT5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000363645.
Polymorphism databases
DMDM	74741070.
Proteomic databases
PaxDb	Q58HT5.
PRIDE	Q58HT5.
Protocols and materials databases
DNASU	158833.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000374521; ENSP00000363645; ENSG00000204195.
GeneID	158833.
KEGG	hsa:158833.
UCSC	uc004dxy.3. human.
Organism-specific databases
CTD	158833.
GeneCards	GC0XP069455.
HGNC	HGNC:23252. AWAT1.
neXtProt	NX_Q58HT5.
PharmGKB	PA164716409.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238369.
HOGENOM	HOG000179738.
HOVERGEN	HBG065791.
InParanoid	Q58HT5.
KO	K11171.
OMA	NYLMGVH.
OrthoDB	EOG498V14.
Enzyme and pathway databases
BRENDA	2.3.1.75. 2681.
Gene expression databases
Bgee	Q58HT5.
CleanEx	HS_DGAT2L3.
Genevestigator	Q58HT5.
GermOnline	ENSG00000204195. Homo sapiens.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. PTHR12317. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	158833.
NextBio	87826.

Entry information

Entry name

AWAT1_HUMAN

Accession

Primary (citable) accession number: Q58HT5
Secondary accession number(s): Q5JT21, Q6IEE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.