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Protein

Acyl-CoA wax alcohol acyltransferase 1

Gene

AWAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference for arachidyl alcohol as well as decyl alcohol, demonstrating its relatively poor activity using saturated long chain alcohols (C16, C18, and C20).1 Publication

Catalytic activityi

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.

Kineticsi

    1. Vmax=31.7 pmol/min/mg enzyme with [14C]oleoyl-CoA as substrate1 Publication
    2. Vmax=113 pmol/min/mg enzyme with [14C]cetyl-CoA as substrate1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi2.3.1.75. 2681.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-CoA wax alcohol acyltransferase 1 (EC:2.3.1.75)
    Alternative name(s):
    Diacylglycerol O-acyltransferase 2-like protein 3
    Diacylglycerol acyltransferase 2
    Long-chain-alcohol O-fatty-acyltransferase 1
    Gene namesi
    Name:AWAT1
    Synonyms:DGA2, DGAT2L3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome X

    Organism-specific databases

    HGNCiHGNC:23252. AWAT1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei34 – 5320HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA164716409.

    Polymorphism and mutation databases

    BioMutaiAWAT1.
    DMDMi74741070.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Acyl-CoA wax alcohol acyltransferase 1PRO_0000249051Add
    BLAST

    Proteomic databases

    PaxDbiQ58HT5.
    PRIDEiQ58HT5.

    Expressioni

    Tissue specificityi

    Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis.1 Publication

    Gene expression databases

    BgeeiQ58HT5.
    CleanExiHS_DGAT2L3.
    GenevisibleiQ58HT5. HS.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000363645.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58HT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG238369.
    GeneTreeiENSGT00750000117391.
    HOGENOMiHOG000179738.
    HOVERGENiHBG065791.
    InParanoidiQ58HT5.
    KOiK11171.
    OMAiLQWPLSY.
    OrthoDBiEOG7KH9KB.
    PhylomeDBiQ58HT5.
    TreeFamiTF314707.

    Family and domain databases

    InterProiIPR007130. DAGAT.
    [Graphical view]
    PANTHERiPTHR12317. PTHR12317. 1 hit.
    PfamiPF03982. DAGAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q58HT5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAHSKQPSHF QSLMLLQWPL SYLAIFWILQ PLFVYLLFTS LWPLPVLYFA
    60 70 80 90 100
    WLFLDWKTPE RGGRRSAWVR NWCVWTHIRD YFPITILKTK DLSPEHNYLM
    110 120 130 140 150
    GVHPHGLLTF GAFCNFCTEA TGFSKTFPGI TPHLATLSWF FKIPFVREYL
    160 170 180 190 200
    MAKGVCSVSQ PAINYLLSHG TGNLVGIVVG GVGEALQSVP NTTTLILQKR
    210 220 230 240 250
    KGFVRTALQH GAHLVPTFTF GETEVYDQVL FHKDSRMYKF QSCFRRIFGF
    260 270 280 290 300
    YCCVFYGQSF CQGSTGLLPY SRPIVTVVGE PLPLPQIEKP SQEMVDKYHA
    310 320
    LYMDALHKLF DQHKTHYGCS ETQKLFFL
    Length:328
    Mass (Da):37,759
    Last modified:April 26, 2005 - v1
    Checksum:i51475CFD31667215
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY947638 mRNA. Translation: AAX48018.1.
    AL357752 Genomic DNA. Translation: CAI41493.2.
    BC039181 Genomic DNA. No translation available.
    BN000155 mRNA. Translation: CAD89266.1.
    CCDSiCCDS35321.1.
    RefSeqiNP_001013597.1. NM_001013579.2.
    UniGeneiHs.407641.

    Genome annotation databases

    EnsembliENST00000374521; ENSP00000363645; ENSG00000204195.
    GeneIDi158833.
    KEGGihsa:158833.
    UCSCiuc004dxy.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY947638 mRNA. Translation: AAX48018.1.
    AL357752 Genomic DNA. Translation: CAI41493.2.
    BC039181 Genomic DNA. No translation available.
    BN000155 mRNA. Translation: CAD89266.1.
    CCDSiCCDS35321.1.
    RefSeqiNP_001013597.1. NM_001013579.2.
    UniGeneiHs.407641.

    3D structure databases

    ProteinModelPortaliQ58HT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi9606.ENSP00000363645.

    Chemistry

    BindingDBiQ58HT5.
    ChEMBLiCHEMBL2375205.

    Polymorphism and mutation databases

    BioMutaiAWAT1.
    DMDMi74741070.

    Proteomic databases

    PaxDbiQ58HT5.
    PRIDEiQ58HT5.

    Protocols and materials databases

    DNASUi158833.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000374521; ENSP00000363645; ENSG00000204195.
    GeneIDi158833.
    KEGGihsa:158833.
    UCSCiuc004dxy.3. human.

    Organism-specific databases

    CTDi158833.
    GeneCardsiGC0XP069455.
    HGNCiHGNC:23252. AWAT1.
    MIMi300924. gene.
    neXtProtiNX_Q58HT5.
    PharmGKBiPA164716409.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiNOG238369.
    GeneTreeiENSGT00750000117391.
    HOGENOMiHOG000179738.
    HOVERGENiHBG065791.
    InParanoidiQ58HT5.
    KOiK11171.
    OMAiLQWPLSY.
    OrthoDBiEOG7KH9KB.
    PhylomeDBiQ58HT5.
    TreeFamiTF314707.

    Enzyme and pathway databases

    BRENDAi2.3.1.75. 2681.

    Miscellaneous databases

    GenomeRNAii158833.
    NextBioi87826.
    PROiQ58HT5.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ58HT5.
    CleanExiHS_DGAT2L3.
    GenevisibleiQ58HT5. HS.

    Family and domain databases

    InterProiIPR007130. DAGAT.
    [Graphical view]
    PANTHERiPTHR12317. PTHR12317. 1 hit.
    PfamiPF03982. DAGAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Identification of two novel human Acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily."
      Turkish A.R., Henneberry A.L., Cromley D., Padamsee M., Oelkers P., Bazzi H., Christiano A.M., Billheimer J.T., Sturley S.L.
      J. Biol. Chem. 280:14755-14764(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-328.
      Tissue: Skin.
    4. "Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals."
      Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R.
      Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiAWAT1_HUMAN
    AccessioniPrimary (citable) accession number: Q58HT5
    Secondary accession number(s): Q5JT21, Q6IEE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: April 26, 2005
    Last modified: June 24, 2015
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.