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Q58HT5

- AWAT1_HUMAN

UniProt

Q58HT5 - AWAT1_HUMAN

Protein

Acyl-CoA wax alcohol acyltransferase 1

Gene

AWAT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference for arachidyl alcohol as well as decyl alcohol, demonstrating its relatively poor activity using saturated long chain alcohols (C16, C18, and C20).1 Publication

    Catalytic activityi

    Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.

    Kineticsi

      Vmax=31.7 pmol/min/mg enzyme with [14C]oleoyl-CoA as substrate1 Publication

      Vmax=113 pmol/min/mg enzyme with [14C]cetyl-CoA as substrate1 Publication

      GO - Molecular functioni

      1. long-chain-alcohol O-fatty-acyltransferase activity Source: UniProtKB-EC

      GO - Biological processi

      1. lipid metabolic process Source: UniProtKB-KW

      Keywords - Molecular functioni

      Acyltransferase, Transferase

      Keywords - Biological processi

      Lipid biosynthesis, Lipid metabolism

      Enzyme and pathway databases

      BRENDAi2.3.1.75. 2681.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Acyl-CoA wax alcohol acyltransferase 1 (EC:2.3.1.75)
      Alternative name(s):
      Diacylglycerol O-acyltransferase 2-like protein 3
      Diacylglycerol acyltransferase 2
      Long-chain-alcohol O-fatty-acyltransferase 1
      Gene namesi
      Name:AWAT1
      Synonyms:DGA2, DGAT2L3
      OrganismiHomo sapiens (Human)
      Taxonomic identifieri9606 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
      ProteomesiUP000005640: Chromosome X

      Organism-specific databases

      HGNCiHGNC:23252. AWAT1.

      Subcellular locationi

      GO - Cellular componenti

      1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
      2. integral component of membrane Source: UniProtKB-KW

      Keywords - Cellular componenti

      Endoplasmic reticulum, Membrane

      Pathology & Biotechi

      Organism-specific databases

      PharmGKBiPA164716409.

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 328328Acyl-CoA wax alcohol acyltransferase 1PRO_0000249051Add
      BLAST

      Proteomic databases

      PaxDbiQ58HT5.
      PRIDEiQ58HT5.

      Expressioni

      Tissue specificityi

      Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis.1 Publication

      Gene expression databases

      BgeeiQ58HT5.
      CleanExiHS_DGAT2L3.
      GenevestigatoriQ58HT5.

      Interactioni

      Protein-protein interaction databases

      STRINGi9606.ENSP00000363645.

      Structurei

      3D structure databases

      ProteinModelPortaliQ58HT5.
      ModBaseiSearch...
      MobiDBiSearch...

      Transmembrane

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Transmembranei12 – 3221HelicalSequence AnalysisAdd
      BLAST
      Transmembranei34 – 5320HelicalSequence AnalysisAdd
      BLAST

      Family & Domainsi

      Sequence similaritiesi

      Keywords - Domaini

      Transmembrane, Transmembrane helix

      Phylogenomic databases

      eggNOGiNOG238369.
      HOGENOMiHOG000179738.
      HOVERGENiHBG065791.
      InParanoidiQ58HT5.
      KOiK11171.
      OMAiFWILQPL.
      OrthoDBiEOG7KH9KB.
      PhylomeDBiQ58HT5.
      TreeFamiTF314707.

      Family and domain databases

      InterProiIPR007130. DAGAT.
      [Graphical view]
      PANTHERiPTHR12317. PTHR12317. 1 hit.
      PfamiPF03982. DAGAT. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Q58HT5-1 [UniParc]FASTAAdd to Basket

      « Hide

      MAHSKQPSHF QSLMLLQWPL SYLAIFWILQ PLFVYLLFTS LWPLPVLYFA    50
      WLFLDWKTPE RGGRRSAWVR NWCVWTHIRD YFPITILKTK DLSPEHNYLM 100
      GVHPHGLLTF GAFCNFCTEA TGFSKTFPGI TPHLATLSWF FKIPFVREYL 150
      MAKGVCSVSQ PAINYLLSHG TGNLVGIVVG GVGEALQSVP NTTTLILQKR 200
      KGFVRTALQH GAHLVPTFTF GETEVYDQVL FHKDSRMYKF QSCFRRIFGF 250
      YCCVFYGQSF CQGSTGLLPY SRPIVTVVGE PLPLPQIEKP SQEMVDKYHA 300
      LYMDALHKLF DQHKTHYGCS ETQKLFFL 328
      Length:328
      Mass (Da):37,759
      Last modified:April 26, 2005 - v1
      Checksum:i51475CFD31667215
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AY947638 mRNA. Translation: AAX48018.1.
      AL357752 Genomic DNA. Translation: CAI41493.2.
      BC039181 Genomic DNA. No translation available.
      BN000155 mRNA. Translation: CAD89266.1.
      CCDSiCCDS35321.1.
      RefSeqiNP_001013597.1. NM_001013579.2.
      UniGeneiHs.407641.

      Genome annotation databases

      EnsembliENST00000374521; ENSP00000363645; ENSG00000204195.
      GeneIDi158833.
      KEGGihsa:158833.
      UCSCiuc004dxy.3. human.

      Polymorphism databases

      DMDMi74741070.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AY947638 mRNA. Translation: AAX48018.1 .
      AL357752 Genomic DNA. Translation: CAI41493.2 .
      BC039181 Genomic DNA. No translation available.
      BN000155 mRNA. Translation: CAD89266.1 .
      CCDSi CCDS35321.1.
      RefSeqi NP_001013597.1. NM_001013579.2.
      UniGenei Hs.407641.

      3D structure databases

      ProteinModelPortali Q58HT5.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 9606.ENSP00000363645.

      Chemistry

      ChEMBLi CHEMBL2375205.

      Polymorphism databases

      DMDMi 74741070.

      Proteomic databases

      PaxDbi Q58HT5.
      PRIDEi Q58HT5.

      Protocols and materials databases

      DNASUi 158833.
      Structural Biology Knowledgebase Search...

      Genome annotation databases

      Ensembli ENST00000374521 ; ENSP00000363645 ; ENSG00000204195 .
      GeneIDi 158833.
      KEGGi hsa:158833.
      UCSCi uc004dxy.3. human.

      Organism-specific databases

      CTDi 158833.
      GeneCardsi GC0XP069455.
      HGNCi HGNC:23252. AWAT1.
      MIMi 300924. gene.
      neXtProti NX_Q58HT5.
      PharmGKBi PA164716409.
      GenAtlasi Search...

      Phylogenomic databases

      eggNOGi NOG238369.
      HOGENOMi HOG000179738.
      HOVERGENi HBG065791.
      InParanoidi Q58HT5.
      KOi K11171.
      OMAi FWILQPL.
      OrthoDBi EOG7KH9KB.
      PhylomeDBi Q58HT5.
      TreeFami TF314707.

      Enzyme and pathway databases

      BRENDAi 2.3.1.75. 2681.

      Miscellaneous databases

      GenomeRNAii 158833.
      NextBioi 87826.
      PROi Q58HT5.
      SOURCEi Search...

      Gene expression databases

      Bgeei Q58HT5.
      CleanExi HS_DGAT2L3.
      Genevestigatori Q58HT5.

      Family and domain databases

      InterProi IPR007130. DAGAT.
      [Graphical view ]
      PANTHERi PTHR12317. PTHR12317. 1 hit.
      Pfami PF03982. DAGAT. 1 hit.
      [Graphical view ]
      ProtoNeti Search...

      Publicationsi

      1. "Identification of two novel human Acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily."
        Turkish A.R., Henneberry A.L., Cromley D., Padamsee M., Oelkers P., Bazzi H., Christiano A.M., Billheimer J.T., Sturley S.L.
        J. Biol. Chem. 280:14755-14764(2005) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      2. "The DNA sequence of the human X chromosome."
        Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
        , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
        Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
        The MGC Project Team
        Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-328.
        Tissue: Skin.
      4. "Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals."
        Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R.
        Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract]
        Cited for: IDENTIFICATION.

      Entry informationi

      Entry nameiAWAT1_HUMAN
      AccessioniPrimary (citable) accession number: Q58HT5
      Secondary accession number(s): Q5JT21, Q6IEE4
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: September 5, 2006
      Last sequence update: April 26, 2005
      Last modified: October 1, 2014
      This is version 81 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program
      DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. Human chromosome X
        Human chromosome X: entries, gene names and cross-references to MIM
      2. MIM cross-references
        Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
      3. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3