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Q58HT5 (AWAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acyl-CoA wax alcohol acyltransferase 1

EC=2.3.1.75
Alternative name(s):
Diacylglycerol O-acyltransferase 2-like protein 3
Diacylglycerol acyltransferase 2
Long-chain-alcohol O-fatty-acyltransferase 1
Gene names
Name:AWAT1
Synonyms:DGA2, DGAT2L3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference for arachidyl alcohol as well as decyl alcohol, demonstrating its relatively poor activity using saturated long chain alcohols (C16, C18, and C20). Ref.1

Catalytic activity

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis. Ref.1

Sequence similarities

Belongs to the diacylglycerol acyltransferase family.

Biophysicochemical properties

Kinetic parameters:

Vmax=31.7 pmol/min/mg enzyme with [14C]oleoyl-CoA as substrate Ref.1

Vmax=113 pmol/min/mg enzyme with [14C]cetyl-CoA as substrate

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Acyl-CoA wax alcohol acyltransferase 1
PRO_0000249051

Regions

Transmembrane12 – 3221Helical; Potential
Transmembrane34 – 5320Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q58HT5 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 51475CFD31667215

FASTA32837,759
        10         20         30         40         50         60 
MAHSKQPSHF QSLMLLQWPL SYLAIFWILQ PLFVYLLFTS LWPLPVLYFA WLFLDWKTPE 

        70         80         90        100        110        120 
RGGRRSAWVR NWCVWTHIRD YFPITILKTK DLSPEHNYLM GVHPHGLLTF GAFCNFCTEA 

       130        140        150        160        170        180 
TGFSKTFPGI TPHLATLSWF FKIPFVREYL MAKGVCSVSQ PAINYLLSHG TGNLVGIVVG 

       190        200        210        220        230        240 
GVGEALQSVP NTTTLILQKR KGFVRTALQH GAHLVPTFTF GETEVYDQVL FHKDSRMYKF 

       250        260        270        280        290        300 
QSCFRRIFGF YCCVFYGQSF CQGSTGLLPY SRPIVTVVGE PLPLPQIEKP SQEMVDKYHA 

       310        320 
LYMDALHKLF DQHKTHYGCS ETQKLFFL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two novel human Acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily."
Turkish A.R., Henneberry A.L., Cromley D., Padamsee M., Oelkers P., Bazzi H., Christiano A.M., Billheimer J.T., Sturley S.L.
J. Biol. Chem. 280:14755-14764(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-328.
Tissue: Skin.
[4]"Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals."
Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R.
Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY947638 mRNA. Translation: AAX48018.1.
AL357752 Genomic DNA. Translation: CAI41493.2.
BC039181 Genomic DNA. No translation available.
BN000155 mRNA. Translation: CAD89266.1.
CCDSCCDS35321.1.
RefSeqNP_001013597.1. NM_001013579.2.
UniGeneHs.407641.

3D structure databases

ProteinModelPortalQ58HT5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000363645.

Chemistry

ChEMBLCHEMBL2375205.

Polymorphism databases

DMDM74741070.

Proteomic databases

PaxDbQ58HT5.
PRIDEQ58HT5.

Protocols and materials databases

DNASU158833.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374521; ENSP00000363645; ENSG00000204195.
GeneID158833.
KEGGhsa:158833.
UCSCuc004dxy.3. human.

Organism-specific databases

CTD158833.
GeneCardsGC0XP069455.
HGNCHGNC:23252. AWAT1.
neXtProtNX_Q58HT5.
PharmGKBPA164716409.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG238369.
HOGENOMHOG000179738.
HOVERGENHBG065791.
InParanoidQ58HT5.
KOK11171.
OMAFWILQPL.
OrthoDBEOG7KH9KB.
PhylomeDBQ58HT5.
TreeFamTF314707.

Enzyme and pathway databases

BRENDA2.3.1.75. 2681.

Gene expression databases

BgeeQ58HT5.
CleanExHS_DGAT2L3.
GenevestigatorQ58HT5.

Family and domain databases

InterProIPR007130. DAGAT.
[Graphical view]
PANTHERPTHR12317. PTHR12317. 1 hit.
PfamPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi158833.
NextBio87826.
PROQ58HT5.

Entry information

Entry nameAWAT1_HUMAN
AccessionPrimary (citable) accession number: Q58HT5
Secondary accession number(s): Q5JT21, Q6IEE4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: April 26, 2005
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM