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Q58F21 (BRDT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bromodomain testis-specific protein
Alternative name(s):
Cancer/testis antigen 9
Short name=CT9
RING3-like protein
Gene names
Name:BRDT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length947 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. Ref.1 Ref.2 Ref.12

Subunit structure

Interacts with mRNA splicing machinery proteins SRSF2, DDX5, HNRNPK and TARDBP. Interacts with the acetylated N-terminus of histone H1, H2, H3 and H4. Interacts with P-TEFb components CDK9 and CCNT1/cyclin-T1. Interacts with SMARCE1 By similarity. Interacts with the acetylated N-terminus of histone H1.4, H2A, H2B, H3 and H4. Ref.11

Subcellular location

Nucleus. Note: Detected on chromatin. Ref.9 Ref.10 Ref.12

Tissue specificity

Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expression is seen in patients' testes with abnormal spermatogenesis. Expressed in cancers such as non-small cell lung cancer and squamous cell carcinomas of the head and neck as well as of esophagus, but not in melanoma or in cancers of the colon, breast, kidney and bladder. Ref.1 Ref.2 Ref.7

Developmental stage

Expressed in embryo testis.

Domain

Bromo domains mediate interaction with histones that have acetylated lysine residues at specific positions (Ref.11). Bromo domain 1 mediates binding with histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) (Ref.12). Ref.11

Miscellaneous

BRDT is a promising target for male contraception. Inhibition by thienodiazepine inhibitor (+)-JQ1 that binds Asn-109, prevents recognition of acetylated histone H4, causing a complete and reversible contraceptive effect in male mice (Ref.12).

Sequence similarities

Contains 2 bromo domains.

Contains 1 NET domain.

Sequence caution

The sequence AAH05281.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH47900.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH62700.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
   Biological processDifferentiation
Meiosis
mRNA processing
mRNA splicing
Spermatogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainBromodomain
Coiled coil
Repeat
   Molecular functionActivator
Chromatin regulator
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

chromatin remodeling

Inferred from sequence or structural similarity. Source: UniProtKB

histone displacement

Inferred from sequence or structural similarity. Source: UniProtKB

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

male meiosis

Inferred from sequence or structural similarity. Source: UniProtKB

male meiosis I

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription during meiosis

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of RNA splicing

Inferred from sequence or structural similarity. Source: UniProtKB

spermatogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionhistone acetyl-lysine binding

Inferred from sequence or structural similarity. Source: UniProtKB

histone binding

Inferred from direct assay Ref.11. Source: UniProtKB

transcription coactivator activity

Traceable author statement PubMed 10365964. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q58F21-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q58F21-2)

Also known as: BRDT-NY;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTCTCRQDSKQLRM
Isoform 3 (identifier: Q58F21-3)

The sequence of this isoform differs from the canonical sequence as follows:
     148-148: K → KGKAG
Isoform 4 (identifier: Q58F21-4)

The sequence of this isoform differs from the canonical sequence as follows:
     65-110: Missing.
Isoform 5 (identifier: Q58F21-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 947947Bromodomain testis-specific protein
PRO_0000239225

Regions

Domain44 – 11673Bromo 1
Domain287 – 35973Bromo 2
Domain500 – 58283NET
Coiled coil417 – 47054 Potential
Coiled coil591 – 62131 Potential
Motif209 – 22012Nuclear localization signal Ref.10
Compositional bias635 – 66733Ser-rich

Sites

Binding site1091Histone H4K5ac By similarity
Binding site1091JQ1 inhibitor
Binding site1141Histone H4K5ac By similarity

Natural variations

Alternative sequence1 – 7373Missing in isoform 5.
VSP_044509
Alternative sequence11M → MTCTCRQDSKQLRM in isoform 2.
VSP_019118
Alternative sequence65 – 11046Missing in isoform 4.
VSP_044510
Alternative sequence1481K → KGKAG in isoform 3.
VSP_044511
Natural variant21S → F. Ref.8
Corresponds to variant rs55806733 [ dbSNP | Ensembl ].
VAR_041924
Natural variant61R → Q. Ref.8
Corresponds to variant rs56273490 [ dbSNP | Ensembl ].
VAR_041925
Natural variant621Q → K. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6
Corresponds to variant rs10783071 [ dbSNP | Ensembl ].
VAR_026584
Natural variant891A → V in a gastric adenocarcinoma sample; somatic mutation. Ref.8
VAR_041926
Natural variant2381K → N. Ref.6 Ref.8
Corresponds to variant rs1156281 [ dbSNP | Ensembl ].
VAR_026585
Natural variant2881H → Y in a lung neuroendocrine carcinoma sample; somatic mutation. Ref.8
VAR_041927
Natural variant3361K → T.
Corresponds to variant rs1064567 [ dbSNP | Ensembl ].
VAR_047327
Natural variant3571E → K. Ref.8
Corresponds to variant rs34674879 [ dbSNP | Ensembl ].
VAR_041928
Natural variant4101N → K. Ref.6 Ref.8
Corresponds to variant rs3088232 [ dbSNP | Ensembl ].
VAR_026586
Natural variant5421P → A. Ref.8
Corresponds to variant rs55912588 [ dbSNP | Ensembl ].
VAR_041929
Natural variant6051R → Q.
Corresponds to variant rs35327986 [ dbSNP | Ensembl ].
VAR_047328
Natural variant6961P → L. Ref.1 Ref.2 Ref.3 Ref.5
Corresponds to variant rs10747493 [ dbSNP | Ensembl ].
VAR_047329

Experimental info

Sequence conflict421D → N in BAH13876. Ref.3
Sequence conflict731P → S in AAH05281. Ref.6
Sequence conflict801K → I in AAH62700. Ref.6
Sequence conflict2651K → E in AAB87862. Ref.1
Sequence conflict3361K → S in AAB87862. Ref.1
Sequence conflict4621E → K in AAH17582. Ref.6
Sequence conflict4771Q → R in BAH13876. Ref.3
Sequence conflict5521I → M in AAQ16198. Ref.2
Sequence conflict6861N → H in AAB87862. Ref.1
Sequence conflict8931Q → P in AAB87862. Ref.1
Sequence conflict9151Q → P in AAB87862. Ref.1

Secondary structure

.................. 947
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 14, 2009. Version 4.
Checksum: 09C0FD8690E217BB

FASTA947107,954
        10         20         30         40         50         60 
MSLPSRQTAI IVNPPPPEYI NTKKNGRLTN QLQYLQKVVL KDLWKHSFSW PFQRPVDAVK 

        70         80         90        100        110        120 
LQLPDYYTII KNPMDLNTIK KRLENKYYAK ASECIEDFNT MFSNCYLYNK PGDDIVLMAQ 

       130        140        150        160        170        180 
ALEKLFMQKL SQMPQEEQVV GVKERIKKGT QQNIAVSSAK EKSSPSATEK VFKQQEIPSV 

       190        200        210        220        230        240 
FPKTSISPLN VVQGASVNSS SQTAAQVTKG VKRKADTTTP ATSAVKASSE FSPTFTEKSV 

       250        260        270        280        290        300 
ALPPIKENMP KNVLPDSQQQ YNVVKTVKVT EQLRHCSEIL KEMLAKKHFS YAWPFYNPVD 

       310        320        330        340        350        360 
VNALGLHNYY DVVKNPMDLG TIKEKMDNQE YKDAYKFAAD VRLMFMNCYK YNPPDHEVVT 

       370        380        390        400        410        420 
MARMLQDVFE THFSKIPIEP VESMPLCYIK TDITETTGRE NTNEASSEGN SSDDSEDERV 

       430        440        450        460        470        480 
KRLAKLQEQL KAVHQQLQVL SQVPFRKLNK KKEKSKKEKK KEKVNNSNEN PRKMCEQMRL 

       490        500        510        520        530        540 
KEKSKRNQPK KRKQQFIGLK SEDEDNAKPM NYDEKRQLSL NINKLPGDKL GRVVHIIQSR 

       550        560        570        580        590        600 
EPSLSNSNPD EIEIDFETLK ASTLRELEKY VSACLRKRPL KPPAKKIMMS KEELHSQKKQ 

       610        620        630        640        650        660 
ELEKRLLDVN NQLNSRKRQT KSDKTQPSKA VENVSRLSES SSSSSSSSES ESSSSDLSSS 

       670        680        690        700        710        720 
DSSDSESEMF PKFTEVKPND SPSKENVKKM KNECIPPEGR TGVTQIGYCV QDTTSANTTL 

       730        740        750        760        770        780 
VHQTTPSHVM PPNHHQLAFN YQELEHLQTV KNISPLQILP PSGDSEQLSN GITVMHPSGD 

       790        800        810        820        830        840 
SDTTMLESEC QAPVQKDIKI KNADSWKSLG KPVKPSGVMK SSDELFNQFR KAAIEKEVKA 

       850        860        870        880        890        900 
RTQELIRKHL EQNTKELKAS QENQRDLGNG LTVESFSNKI QNKCSGEEQK EHQQSSEAQD 

       910        920        930        940 
KSKLWLLKDR DLARQKEQER RRREAMVGTI DMTLQSDIMT MFENNFD 

« Hide

Isoform 2 (BRDT-NY) [UniParc].

Checksum: D477F897F6D72935
Show »

FASTA960109,506
Isoform 3 [UniParc].

Checksum: 8A9508DF12C6AB36
Show »

FASTA951108,268
Isoform 4 [UniParc].

Checksum: 64B19CBC80791B60
Show »

FASTA901102,361
Isoform 5 [UniParc].

Checksum: C8A7FAC55BA14F94
Show »

FASTA87499,394

References

« Hide 'large scale' references
[1]"Identification and characterization of BRDT: a testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh."
Jones M.H., Numata M., Shimane M.
Genomics 45:529-534(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANTS LYS-62 AND LEU-696.
[2]"Molecular cloning and expression of a novel alternative splice variant of BRDT gene."
Zheng Y., Yuan W., Zhou Z., Xu M., Sha J.-H.
Int. J. Mol. Med. 15:315-321(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, VARIANTS LYS-62 AND LEU-696.
Tissue: Testis.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), VARIANTS LYS-62 AND LEU-696.
Tissue: Testis.
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS LYS-62 AND LEU-696.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-462 (ISOFORM 1), VARIANTS LYS-62; ASN-238 AND LYS-410.
Tissue: Brain and Testis.
[7]"Expression of cancer-testis antigens in lung cancer: definition of bromodomain testis-specific gene (BRDT) as a new CT gene, CT9."
Scanlan M.J., Altorki N.K., Gure A.O., Williamson B., Jungbluth A., Chen Y.-T., Old L.J.
Cancer Lett. 150:155-164(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
[8]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] PHE-2; GLN-6; VAL-89; ASN-238; TYR-288; LYS-357; LYS-410 AND ALA-542.
[9]"The interaction of modified histones with the bromodomain testis-specific (BRDT) gene and its mRNA level in sperm of fertile donors and subfertile men."
Steilmann C., Cavalcanti M.C., Bartkuhn M., Pons-Kuhnemann J., Schuppe H.C., Weidner W., Steger K., Paradowska A.
Reproduction 140:435-443(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"The conserved 12-amino acid stretch in the inter-bromodomain region of BET family proteins functions as a nuclear localization signal."
Fukazawa H., Masumi A.
Biol. Pharm. Bull. 35:2064-2068(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MOTIF NUCLEAR LOCALIZATION SIGNAL.
[11]"Histone recognition and large-scale structural analysis of the human bromodomain family."
Filippakopoulos P., Picaud S., Mangos M., Keates T., Lambert J.P., Barsyte-Lovejoy D., Felletar I., Volkmer R., Muller S., Pawson T., Gingras A.C., Arrowsmith C.H., Knapp S.
Cell 149:214-231(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 21-137, SUBUNIT, DOMAIN.
[12]"Small-molecule inhibition of BRDT for male contraception."
Matzuk M.M., McKeown M.R., Filippakopoulos P., Li Q., Ma L., Agno J.E., Lemieux M.E., Picaud S., Yu R.N., Qi J., Knapp S., Bradner J.E.
Cell 150:673-684(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.23 ANGSTROMS) OF 21-137 IN COMPLEX WITH JQ1, SUBCELLULAR LOCATION, FUNCTION.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Asking life to be patient - Issue 144 of November 2012

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019085 mRNA. Translation: AAB87862.1.
AY338951 mRNA. Translation: AAQ16198.1.
AK303008 mRNA. Translation: BAH13876.1.
AK302758 mRNA. Translation: BAH13797.1.
AK316442 mRNA. Translation: BAH14813.1.
AC114486 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73106.1.
CH471097 Genomic DNA. Translation: EAW73107.1.
CH471097 Genomic DNA. Translation: EAW73108.1.
CH471097 Genomic DNA. Translation: EAW73110.1.
CH471097 Genomic DNA. Translation: EAW73111.1.
BC005281 mRNA. Translation: AAH05281.1. Sequence problems.
BC017582 mRNA. Translation: AAH17582.1.
BC047900 mRNA. Translation: AAH47900.1. Sequence problems.
BC062700 mRNA. Translation: AAH62700.1. Sequence problems.
RefSeqNP_001229734.1. NM_001242805.1.
NP_001229735.1. NM_001242806.1.
NP_001229736.1. NM_001242807.1.
NP_001229737.1. NM_001242808.1.
NP_001229739.1. NM_001242810.1.
NP_001717.2. NM_001726.3.
NP_997072.1. NM_207189.2.
XP_005271208.1. XM_005271151.2.
UniGeneHs.482520.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2RFJX-ray2.05A/B/C21-137[»]
4FLPX-ray2.23A/B21-137[»]
4KCXX-ray2.00A/B21-137[»]
ProteinModelPortalQ58F21.
SMRQ58F21. Positions 29-134, 266-377, 505-578.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107143. 4 interactions.
IntActQ58F21. 1 interaction.
STRING9606.ENSP00000354568.

Chemistry

ChEMBLCHEMBL1795185.

PTM databases

PhosphoSiteQ58F21.

Polymorphism databases

DMDM226694198.

Proteomic databases

PaxDbQ58F21.
PRIDEQ58F21.

Protocols and materials databases

DNASU676.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000362005; ENSP00000354568; ENSG00000137948. [Q58F21-1]
ENST00000370389; ENSP00000359416; ENSG00000137948. [Q58F21-5]
ENST00000394530; ENSP00000378038; ENSG00000137948. [Q58F21-4]
ENST00000399546; ENSP00000387822; ENSG00000137948. [Q58F21-1]
ENST00000402388; ENSP00000384051; ENSG00000137948. [Q58F21-1]
GeneID676.
KEGGhsa:676.
UCSCuc001dok.4. human. [Q58F21-1]
uc010osz.2. human. [Q58F21-3]

Organism-specific databases

CTD676.
GeneCardsGC01P092414.
HGNCHGNC:1105. BRDT.
HPACAB012237.
HPA015283.
MIM602144. gene.
neXtProtNX_Q58F21.
PharmGKBPA25418.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5076.
InParanoidQ58F21.
KOK11724.
OMAENQRDLG.
OrthoDBEOG7TTQ86.
PhylomeDBQ58F21.
TreeFamTF317345.

Gene expression databases

ArrayExpressQ58F21.
BgeeQ58F21.
CleanExHS_BRDT.
GenevestigatorQ58F21.

Family and domain databases

Gene3D1.20.920.10. 2 hits.
InterProIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamPF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMSSF47370. SSF47370. 2 hits.
PROSITEPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBRDT. human.
EvolutionaryTraceQ58F21.
GeneWikiBRDT.
GenomeRNAi676.
NextBio2784.
PROQ58F21.
SOURCESearch...

Entry information

Entry nameBRDT_HUMAN
AccessionPrimary (citable) accession number: Q58F21
Secondary accession number(s): A6NF68 expand/collapse secondary AC list , B7Z811, B7Z890, B7ZAX7, D3DT32, O14789, Q05DQ4, Q6P5T1, Q7Z4A6, Q8IWI6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: April 14, 2009
Last modified: April 16, 2014
This is version 87 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM