Q58F21 (BRDT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bromodomain testis-specific protein

Alternative name(s):
Cancer/testis antigen 9
Short name=CT9
RING3-like protein

Gene names

Name:

BRDT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	947 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. Ref.1 Ref.2 Ref.12
Subunit structure	Interacts with mRNA splicing machinery proteins SRSF2, DDX5, HNRNPK and TARDBP. Interacts with the acetylated N-terminus of histone H1, H2, H3 and H4. Interacts with P-TEFb components CDK9 and CCNT1/cyclin-T1. Interacts with SMARCE1 By similarity. Interacts with the acetylated N-terminus of histone H1.4, H2A, H2B, H3 and H4. Ref.11
Subcellular location	Nucleus. Note: Detected on chromatin. Ref.9 Ref.10 Ref.12
Tissue specificity	Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expression is seen in patients' testes with abnormal spermatogenesis. Expressed in cancers such as non-small cell lung cancer and squamous cell carcinomas of the head and neck as well as of esophagus, but not in melanoma or in cancers of the colon, breast, kidney and bladder. Ref.1 Ref.2 Ref.7
Developmental stage	Expressed in embryo testis.
Domain	Bromo domains mediate interaction with histones that have acetylated lysine residues at specific positions (Ref.11). Bromo domain 1 mediates binding with histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) (Ref.12). Ref.11
Miscellaneous	BRDT is a promising target for male contraception. Inhibition by thienodiazepine inhibitor (+)-JQ1 that binds Asn-109, prevents recognition of acetylated histone H4, causing a complete and reversible contraceptive effect in male mice (Ref.12).
Sequence similarities	Contains 2 bromo domains. Contains 1 NET domain.
Sequence caution	The sequence AAH05281.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH47900.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH62700.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Differentiation Meiosis Spermatogenesis Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Bromodomain Coiled coil Repeat
Molecular function	Activator Chromatin regulator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW chromatin remodeling Inferred from sequence or structural similarity. Source: UniProtKB histone displacement Inferred from sequence or structural similarity. Source: UniProtKB mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW male meiosis I Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription during meiosis Inferred from sequence or structural similarity. Source: UniProtKB regulation of RNA splicing Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	histone acetyl-lysine binding Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Traceable author statement PubMed 10365964. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q58F21-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q58F21-2)

Also known as: BRDT-NY;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTCTCRQDSKQLRM

Isoform 3 (identifier: Q58F21-3)

The sequence of this isoform differs from the canonical sequence as follows:
     148-148: K → KGKAG

Isoform 4 (identifier: Q58F21-4)

The sequence of this isoform differs from the canonical sequence as follows:
     65-110: Missing.

Isoform 5 (identifier: Q58F21-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 947

947

Bromodomain testis-specific protein

PRO_0000239225

Regions

Domain

44 – 116

73

Bromo 1

Domain

287 – 359

73

Bromo 2

Domain

500 – 582

83

NET

Coiled coil

417 – 470

54

Potential

Coiled coil

591 – 621

31

Potential

Motif

209 – 220

12

Nuclear localization signal Ref.10

Compositional bias

635 – 667

33

Ser-rich

Sites

Binding site

109

1

Histone H4K5ac By similarity

Binding site

109

1

JQ1 inhibitor

Binding site

114

1

Histone H4K5ac By similarity

Natural variations

Alternative sequence

1 – 73

73

Missing in isoform 5.

VSP_044509

Alternative sequence

1

1

M → MTCTCRQDSKQLRM in isoform 2.

VSP_019118

Alternative sequence

65 – 110

46

Missing in isoform 4.

VSP_044510

Alternative sequence

148

1

K → KGKAG in isoform 3.

VSP_044511

Natural variant

2

1

S → F. Ref.8
Corresponds to variant rs55806733 [ dbSNP | Ensembl ].

VAR_041924

Natural variant

6

1

R → Q. Ref.8
Corresponds to variant rs56273490 [ dbSNP | Ensembl ].

VAR_041925

Natural variant

62

1

Q → K. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6
Corresponds to variant rs10783071 [ dbSNP | Ensembl ].

VAR_026584

Natural variant

89

1

A → V in a gastric adenocarcinoma sample; somatic mutation. Ref.8

VAR_041926

Natural variant

238

1

K → N. Ref.6 Ref.8
Corresponds to variant rs1156281 [ dbSNP | Ensembl ].

VAR_026585

Natural variant

288

1

H → Y in a lung neuroendocrine carcinoma sample; somatic mutation. Ref.8

VAR_041927

Natural variant

336

1

K → T.
Corresponds to variant rs1064567 [ dbSNP | Ensembl ].

VAR_047327

Natural variant

357

1

E → K. Ref.8
Corresponds to variant rs34674879 [ dbSNP | Ensembl ].

VAR_041928

Natural variant

410

1

N → K. Ref.6 Ref.8
Corresponds to variant rs3088232 [ dbSNP | Ensembl ].

VAR_026586

Natural variant

542

1

P → A. Ref.8
Corresponds to variant rs55912588 [ dbSNP | Ensembl ].

VAR_041929

Natural variant

605

1

R → Q.
Corresponds to variant rs35327986 [ dbSNP | Ensembl ].

VAR_047328

Natural variant

696

1

P → L. Ref.1 Ref.2 Ref.3 Ref.5
Corresponds to variant rs10747493 [ dbSNP | Ensembl ].

VAR_047329

Experimental info

Sequence conflict

42

1

D → N in BAH13876. Ref.3

Sequence conflict

73

1

P → S in AAH05281. Ref.6

Sequence conflict

80

1

K → I in AAH62700. Ref.6

Sequence conflict

265

1

K → E in AAB87862. Ref.1

Sequence conflict

336

1

K → S in AAB87862. Ref.1

Sequence conflict

462

1

E → K in AAH17582. Ref.6

Sequence conflict

477

1

Q → R in BAH13876. Ref.3

Sequence conflict

552

1

I → M in AAQ16198. Ref.2

Sequence conflict

686

1

N → H in AAB87862. Ref.1

Sequence conflict

893

1

Q → P in AAB87862. Ref.1

Sequence conflict

915

1

Q → P in AAB87862. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

947

Helix Strand Turn

Details...

Helix

28 – 37

10

Helix

39 – 45

7

Helix

50 – 52

3

Beta strand

53 – 55

3

Helix

58 – 61

4

Helix

63 – 70

8

Helix

76 – 84

9

Helix

91 – 108

18

Helix

114 – 131

18

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 4. Checksum: 09C0FD8690E217BB	FASTA	947	107,954
	10 20 30 40 50 60 MSLPSRQTAI IVNPPPPEYI NTKKNGRLTN QLQYLQKVVL KDLWKHSFSW PFQRPVDAVK 70 80 90 100 110 120 LQLPDYYTII KNPMDLNTIK KRLENKYYAK ASECIEDFNT MFSNCYLYNK PGDDIVLMAQ 130 140 150 160 170 180 ALEKLFMQKL SQMPQEEQVV GVKERIKKGT QQNIAVSSAK EKSSPSATEK VFKQQEIPSV 190 200 210 220 230 240 FPKTSISPLN VVQGASVNSS SQTAAQVTKG VKRKADTTTP ATSAVKASSE FSPTFTEKSV 250 260 270 280 290 300 ALPPIKENMP KNVLPDSQQQ YNVVKTVKVT EQLRHCSEIL KEMLAKKHFS YAWPFYNPVD 310 320 330 340 350 360 VNALGLHNYY DVVKNPMDLG TIKEKMDNQE YKDAYKFAAD VRLMFMNCYK YNPPDHEVVT 370 380 390 400 410 420 MARMLQDVFE THFSKIPIEP VESMPLCYIK TDITETTGRE NTNEASSEGN SSDDSEDERV 430 440 450 460 470 480 KRLAKLQEQL KAVHQQLQVL SQVPFRKLNK KKEKSKKEKK KEKVNNSNEN PRKMCEQMRL 490 500 510 520 530 540 KEKSKRNQPK KRKQQFIGLK SEDEDNAKPM NYDEKRQLSL NINKLPGDKL GRVVHIIQSR 550 560 570 580 590 600 EPSLSNSNPD EIEIDFETLK ASTLRELEKY VSACLRKRPL KPPAKKIMMS KEELHSQKKQ 610 620 630 640 650 660 ELEKRLLDVN NQLNSRKRQT KSDKTQPSKA VENVSRLSES SSSSSSSSES ESSSSDLSSS 670 680 690 700 710 720 DSSDSESEMF PKFTEVKPND SPSKENVKKM KNECIPPEGR TGVTQIGYCV QDTTSANTTL 730 740 750 760 770 780 VHQTTPSHVM PPNHHQLAFN YQELEHLQTV KNISPLQILP PSGDSEQLSN GITVMHPSGD 790 800 810 820 830 840 SDTTMLESEC QAPVQKDIKI KNADSWKSLG KPVKPSGVMK SSDELFNQFR KAAIEKEVKA 850 860 870 880 890 900 RTQELIRKHL EQNTKELKAS QENQRDLGNG LTVESFSNKI QNKCSGEEQK EHQQSSEAQD 910 920 930 940 KSKLWLLKDR DLARQKEQER RRREAMVGTI DMTLQSDIMT MFENNFD
	Isoform 2 (BRDT-NY) [UniParc]. Checksum: D477F897F6D72935	FASTA	960	109,506
	10 20 30 40 50 60 MTCTCRQDSK QLRMSLPSRQ TAIIVNPPPP EYINTKKNGR LTNQLQYLQK VVLKDLWKHS 70 80 90 100 110 120 FSWPFQRPVD AVKLQLPDYY TIIKNPMDLN TIKKRLENKY YAKASECIED FNTMFSNCYL 130 140 150 160 170 180 YNKPGDDIVL MAQALEKLFM QKLSQMPQEE QVVGVKERIK KGTQQNIAVS SAKEKSSPSA 190 200 210 220 230 240 TEKVFKQQEI PSVFPKTSIS PLNVVQGASV NSSSQTAAQV TKGVKRKADT TTPATSAVKA 250 260 270 280 290 300 SSEFSPTFTE KSVALPPIKE NMPKNVLPDS QQQYNVVKTV KVTEQLRHCS EILKEMLAKK 310 320 330 340 350 360 HFSYAWPFYN PVDVNALGLH NYYDVVKNPM DLGTIKEKMD NQEYKDAYKF AADVRLMFMN 370 380 390 400 410 420 CYKYNPPDHE VVTMARMLQD VFETHFSKIP IEPVESMPLC YIKTDITETT GRENTNEASS 430 440 450 460 470 480 EGNSSDDSED ERVKRLAKLQ EQLKAVHQQL QVLSQVPFRK LNKKKEKSKK EKKKEKVNNS 490 500 510 520 530 540 NENPRKMCEQ MRLKEKSKRN QPKKRKQQFI GLKSEDEDNA KPMNYDEKRQ LSLNINKLPG 550 560 570 580 590 600 DKLGRVVHII QSREPSLSNS NPDEIEIDFE TLKASTLREL EKYVSACLRK RPLKPPAKKI 610 620 630 640 650 660 MMSKEELHSQ KKQELEKRLL DVNNQLNSRK RQTKSDKTQP SKAVENVSRL SESSSSSSSS 670 680 690 700 710 720 SESESSSSDL SSSDSSDSES EMFPKFTEVK PNDSPSKENV KKMKNECIPP EGRTGVTQIG 730 740 750 760 770 780 YCVQDTTSAN TTLVHQTTPS HVMPPNHHQL AFNYQELEHL QTVKNISPLQ ILPPSGDSEQ 790 800 810 820 830 840 LSNGITVMHP SGDSDTTMLE SECQAPVQKD IKIKNADSWK SLGKPVKPSG VMKSSDELFN 850 860 870 880 890 900 QFRKAAIEKE VKARTQELIR KHLEQNTKEL KASQENQRDL GNGLTVESFS NKIQNKCSGE 910 920 930 940 950 960 EQKEHQQSSE AQDKSKLWLL KDRDLARQKE QERRRREAMV GTIDMTLQSD IMTMFENNFD
	Isoform 3 [UniParc]. Checksum: 8A9508DF12C6AB36	FASTA	951	108,268
	10 20 30 40 50 60 MSLPSRQTAI IVNPPPPEYI NTKKNGRLTN QLQYLQKVVL KDLWKHSFSW PFQRPVDAVK 70 80 90 100 110 120 LQLPDYYTII KNPMDLNTIK KRLENKYYAK ASECIEDFNT MFSNCYLYNK PGDDIVLMAQ 130 140 150 160 170 180 ALEKLFMQKL SQMPQEEQVV GVKERIKKGK AGGTQQNIAV SSAKEKSSPS ATEKVFKQQE 190 200 210 220 230 240 IPSVFPKTSI SPLNVVQGAS VNSSSQTAAQ VTKGVKRKAD TTTPATSAVK ASSEFSPTFT 250 260 270 280 290 300 EKSVALPPIK ENMPKNVLPD SQQQYNVVKT VKVTEQLRHC SEILKEMLAK KHFSYAWPFY 310 320 330 340 350 360 NPVDVNALGL HNYYDVVKNP MDLGTIKEKM DNQEYKDAYK FAADVRLMFM NCYKYNPPDH 370 380 390 400 410 420 EVVTMARMLQ DVFETHFSKI PIEPVESMPL CYIKTDITET TGRENTNEAS SEGNSSDDSE 430 440 450 460 470 480 DERVKRLAKL QEQLKAVHQQ LQVLSQVPFR KLNKKKEKSK KEKKKEKVNN SNENPRKMCE 490 500 510 520 530 540 QMRLKEKSKR NQPKKRKQQF IGLKSEDEDN AKPMNYDEKR QLSLNINKLP GDKLGRVVHI 550 560 570 580 590 600 IQSREPSLSN SNPDEIEIDF ETLKASTLRE LEKYVSACLR KRPLKPPAKK IMMSKEELHS 610 620 630 640 650 660 QKKQELEKRL LDVNNQLNSR KRQTKSDKTQ PSKAVENVSR LSESSSSSSS SSESESSSSD 670 680 690 700 710 720 LSSSDSSDSE SEMFPKFTEV KPNDSPSKEN VKKMKNECIP PEGRTGVTQI GYCVQDTTSA 730 740 750 760 770 780 NTTLVHQTTP SHVMPPNHHQ LAFNYQELEH LQTVKNISPL QILPPSGDSE QLSNGITVMH 790 800 810 820 830 840 PSGDSDTTML ESECQAPVQK DIKIKNADSW KSLGKPVKPS GVMKSSDELF NQFRKAAIEK 850 860 870 880 890 900 EVKARTQELI RKHLEQNTKE LKASQENQRD LGNGLTVESF SNKIQNKCSG EEQKEHQQSS 910 920 930 940 950 EAQDKSKLWL LKDRDLARQK EQERRRREAM VGTIDMTLQS DIMTMFENNF D
	Isoform 4 [UniParc]. Checksum: 64B19CBC80791B60	FASTA	901	102,361
	10 20 30 40 50 60 MSLPSRQTAI IVNPPPPEYI NTKKNGRLTN QLQYLQKVVL KDLWKHSFSW PFQRPVDAVK 70 80 90 100 110 120 LQLPPGDDIV LMAQALEKLF MQKLSQMPQE EQVVGVKERI KKGTQQNIAV SSAKEKSSPS 130 140 150 160 170 180 ATEKVFKQQE IPSVFPKTSI SPLNVVQGAS VNSSSQTAAQ VTKGVKRKAD TTTPATSAVK 190 200 210 220 230 240 ASSEFSPTFT EKSVALPPIK ENMPKNVLPD SQQQYNVVKT VKVTEQLRHC SEILKEMLAK 250 260 270 280 290 300 KHFSYAWPFY NPVDVNALGL HNYYDVVKNP MDLGTIKEKM DNQEYKDAYK FAADVRLMFM 310 320 330 340 350 360 NCYKYNPPDH EVVTMARMLQ DVFETHFSKI PIEPVESMPL CYIKTDITET TGRENTNEAS 370 380 390 400 410 420 SEGNSSDDSE DERVKRLAKL QEQLKAVHQQ LQVLSQVPFR KLNKKKEKSK KEKKKEKVNN 430 440 450 460 470 480 SNENPRKMCE QMRLKEKSKR NQPKKRKQQF IGLKSEDEDN AKPMNYDEKR QLSLNINKLP 490 500 510 520 530 540 GDKLGRVVHI IQSREPSLSN SNPDEIEIDF ETLKASTLRE LEKYVSACLR KRPLKPPAKK 550 560 570 580 590 600 IMMSKEELHS QKKQELEKRL LDVNNQLNSR KRQTKSDKTQ PSKAVENVSR LSESSSSSSS 610 620 630 640 650 660 SSESESSSSD LSSSDSSDSE SEMFPKFTEV KPNDSPSKEN VKKMKNECIP PEGRTGVTQI 670 680 690 700 710 720 GYCVQDTTSA NTTLVHQTTP SHVMPPNHHQ LAFNYQELEH LQTVKNISPL QILPPSGDSE 730 740 750 760 770 780 QLSNGITVMH PSGDSDTTML ESECQAPVQK DIKIKNADSW KSLGKPVKPS GVMKSSDELF 790 800 810 820 830 840 NQFRKAAIEK EVKARTQELI RKHLEQNTKE LKASQENQRD LGNGLTVESF SNKIQNKCSG 850 860 870 880 890 900 EEQKEHQQSS EAQDKSKLWL LKDRDLARQK EQERRRREAM VGTIDMTLQS DIMTMFENNF D
	Isoform 5 [UniParc]. Checksum: C8A7FAC55BA14F94	FASTA	874	99,394
	10 20 30 40 50 60 MDLNTIKKRL ENKYYAKASE CIEDFNTMFS NCYLYNKPGD DIVLMAQALE KLFMQKLSQM 70 80 90 100 110 120 PQEEQVVGVK ERIKKGTQQN IAVSSAKEKS SPSATEKVFK QQEIPSVFPK TSISPLNVVQ 130 140 150 160 170 180 GASVNSSSQT AAQVTKGVKR KADTTTPATS AVKASSEFSP TFTEKSVALP PIKENMPKNV 190 200 210 220 230 240 LPDSQQQYNV VKTVKVTEQL RHCSEILKEM LAKKHFSYAW PFYNPVDVNA LGLHNYYDVV 250 260 270 280 290 300 KNPMDLGTIK EKMDNQEYKD AYKFAADVRL MFMNCYKYNP PDHEVVTMAR MLQDVFETHF 310 320 330 340 350 360 SKIPIEPVES MPLCYIKTDI TETTGRENTN EASSEGNSSD DSEDERVKRL AKLQEQLKAV 370 380 390 400 410 420 HQQLQVLSQV PFRKLNKKKE KSKKEKKKEK VNNSNENPRK MCEQMRLKEK SKRNQPKKRK 430 440 450 460 470 480 QQFIGLKSED EDNAKPMNYD EKRQLSLNIN KLPGDKLGRV VHIIQSREPS LSNSNPDEIE 490 500 510 520 530 540 IDFETLKAST LRELEKYVSA CLRKRPLKPP AKKIMMSKEE LHSQKKQELE KRLLDVNNQL 550 560 570 580 590 600 NSRKRQTKSD KTQPSKAVEN VSRLSESSSS SSSSSESESS SSDLSSSDSS DSESEMFPKF 610 620 630 640 650 660 TEVKPNDSPS KENVKKMKNE CIPPEGRTGV TQIGYCVQDT TSANTTLVHQ TTPSHVMPPN 670 680 690 700 710 720 HHQLAFNYQE LEHLQTVKNI SPLQILPPSG DSEQLSNGIT VMHPSGDSDT TMLESECQAP 730 740 750 760 770 780 VQKDIKIKNA DSWKSLGKPV KPSGVMKSSD ELFNQFRKAA IEKEVKARTQ ELIRKHLEQN 790 800 810 820 830 840 TKELKASQEN QRDLGNGLTV ESFSNKIQNK CSGEEQKEHQ QSSEAQDKSK LWLLKDRDLA 850 860 870 RQKEQERRRR EAMVGTIDMT LQSDIMTMFE NNFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of BRDT: a testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh." Jones M.H., Numata M., Shimane M. Genomics 45:529-534(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANTS LYS-62 AND LEU-696.
[2]	"Molecular cloning and expression of a novel alternative splice variant of BRDT gene." Zheng Y., Yuan W., Zhou Z., Xu M., Sha J.-H. Int. J. Mol. Med. 15:315-321(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, VARIANTS LYS-62 AND LEU-696. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), VARIANTS LYS-62 AND LEU-696. Tissue: Testis.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS LYS-62 AND LEU-696.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-462 (ISOFORM 1), VARIANTS LYS-62; ASN-238 AND LYS-410. Tissue: Brain and Testis.
[7]	"Expression of cancer-testis antigens in lung cancer: definition of bromodomain testis-specific gene (BRDT) as a new CT gene, CT9." Scanlan M.J., Altorki N.K., Gure A.O., Williamson B., Jungbluth A., Chen Y.-T., Old L.J. Cancer Lett. 150:155-164(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] PHE-2; GLN-6; VAL-89; ASN-238; TYR-288; LYS-357; LYS-410 AND ALA-542.
[9]	"The interaction of modified histones with the bromodomain testis-specific (BRDT) gene and its mRNA level in sperm of fertile donors and subfertile men." Steilmann C., Cavalcanti M.C., Bartkuhn M., Pons-Kuhnemann J., Schuppe H.C., Weidner W., Steger K., Paradowska A. Reproduction 140:435-443(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"The conserved 12-amino acid stretch in the inter-bromodomain region of BET family proteins functions as a nuclear localization signal." Fukazawa H., Masumi A. Biol. Pharm. Bull. 35:2064-2068(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MOTIF NUCLEAR LOCALIZATION SIGNAL.
[11]	"Histone recognition and large-scale structural analysis of the human bromodomain family." Filippakopoulos P., Picaud S., Mangos M., Keates T., Lambert J.P., Barsyte-Lovejoy D., Felletar I., Volkmer R., Muller S., Pawson T., Gingras A.C., Arrowsmith C.H., Knapp S. Cell 149:214-231(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 21-137, SUBUNIT, DOMAIN.
[12]	"Small-molecule inhibition of BRDT for male contraception." Matzuk M.M., McKeown M.R., Filippakopoulos P., Li Q., Ma L., Agno J.E., Lemieux M.E., Picaud S., Yu R.N., Qi J., Knapp S., Bradner J.E. Cell 150:673-684(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.23 ANGSTROMS) OF 21-137 IN COMPLEX WITH JQ1, SUBCELLULAR LOCATION, FUNCTION.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Asking life to be patient - Issue 144 of November 2012

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF019085 mRNA. Translation: AAB87862.1.
AY338951 mRNA. Translation: AAQ16198.1.
AK303008 mRNA. Translation: BAH13876.1.
AK302758 mRNA. Translation: BAH13797.1.
AK316442 mRNA. Translation: BAH14813.1.
AC114486 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73106.1.
CH471097 Genomic DNA. Translation: EAW73107.1.
CH471097 Genomic DNA. Translation: EAW73108.1.
CH471097 Genomic DNA. Translation: EAW73110.1.
CH471097 Genomic DNA. Translation: EAW73111.1.
BC005281 mRNA. Translation: AAH05281.1. Sequence problems.
BC017582 mRNA. Translation: AAH17582.1.
BC047900 mRNA. Translation: AAH47900.1. Sequence problems.
BC062700 mRNA. Translation: AAH62700.1. Sequence problems.

IPI

IPI00479548.
IPI00759691.

RefSeq

NP_001229734.1. NM_001242805.1.
NP_001229735.1. NM_001242806.1.
NP_001229736.1. NM_001242807.1.
NP_001229737.1. NM_001242808.1.
NP_001229739.1. NM_001242810.1.
NP_001717.2. NM_001726.3.
NP_997072.1. NM_207189.2.

UniGene

Hs.482520.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RFJ	X-ray	2.05	A/B/C	21-137	[»]
4FLP	X-ray	2.23	A/B	21-137	[»]

ProteinModelPortal

Q58F21.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q58F21. 1 interaction.

STRING

9606.ENSP00000354568.

PTM databases

PhosphoSite

Q58F21.

Polymorphism databases

DMDM

226694198.

Proteomic databases

PaxDb

Q58F21.

PRIDE

Q58F21.

Protocols and materials databases

DNASU

676.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000362005; ENSP00000354568; ENSG00000137948.
ENST00000370389; ENSP00000359416; ENSG00000137948.
ENST00000394530; ENSP00000378038; ENSG00000137948.
ENST00000399546; ENSP00000387822; ENSG00000137948.
ENST00000402388; ENSP00000384051; ENSG00000137948.

GeneID

676.

KEGG

hsa:676.

UCSC

uc001dok.4. human.

Organism-specific databases

CTD

676.

GeneCards

GC01P092414.

HGNC

HGNC:1105. BRDT.

HPA

CAB012237.
HPA015283.

MIM

602144. gene.

neXtProt

NX_Q58F21.

PharmGKB

PA25418.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5076.

InParanoid

Q58F21.

KO

K11724.

OMA

GVMKSSD.

OrthoDB

EOG4NVZJT.

Gene expression databases

ArrayExpress

Q58F21.

Bgee

Q58F21.

CleanEx

HS_BRDT.

Genevestigator

Q58F21.

GermOnline

ENSG00000137948. Homo sapiens.

Family and domain databases

Gene3D

1.20.920.10. 2 hits.

InterPro

IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]

Pfam

PF00439. Bromodomain. 2 hits.
[Graphical view]

PRINTS

PR00503. BROMODOMAIN.

SMART

SM00297. BROMO. 2 hits.
[Graphical view]

SUPFAM

SSF47370. Bromodomain. 2 hits.

PROSITE

PS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1795185.

ChiTaRS

BRDT. human.

EvolutionaryTrace

Q58F21.

GenomeRNAi

676.

NextBio

2784.

SOURCE

Search...

Entry information

Entry name

BRDT_HUMAN

Accession

Primary (citable) accession number: Q58F21
Secondary accession number(s): A6NF68 , B7Z811, B7Z890, B7ZAX7, D3DT32, O14789, Q05DQ4, Q6P5T1, Q7Z4A6, Q8IWI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 77 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.