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Q58F21

- BRDT_HUMAN

UniProt

Q58F21 - BRDT_HUMAN

Protein

Bromodomain testis-specific protein

Gene

BRDT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 4 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091Histone H4K5acBy similarity
    Binding sitei109 – 1091JQ1 inhibitor1 Publication
    Binding sitei114 – 1141Histone H4K5acBy similarity

    GO - Molecular functioni

    1. histone binding Source: UniProtKB
    2. lysine-acetylated histone binding Source: UniProtKB
    3. transcription coactivator activity Source: ProtInc

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. chromatin remodeling Source: UniProtKB
    3. histone displacement Source: UniProtKB
    4. male meiosis Source: UniProtKB
    5. male meiosis I Source: UniProtKB
    6. mRNA processing Source: UniProtKB-KW
    7. positive regulation of transcription during meiosis Source: UniProtKB
    8. regulation of RNA splicing Source: UniProtKB
    9. RNA splicing Source: UniProtKB-KW
    10. spermatogenesis Source: UniProtKB
    11. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Chromatin regulator

    Keywords - Biological processi

    Differentiation, Meiosis, mRNA processing, mRNA splicing, Spermatogenesis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bromodomain testis-specific protein
    Alternative name(s):
    Cancer/testis antigen 9
    Short name:
    CT9
    RING3-like protein
    Gene namesi
    Name:BRDT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:1105. BRDT.

    Subcellular locationi

    Nucleus 3 Publications
    Note: Detected on chromatin.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25418.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 947947Bromodomain testis-specific proteinPRO_0000239225Add
    BLAST

    Proteomic databases

    MaxQBiQ58F21.
    PaxDbiQ58F21.
    PRIDEiQ58F21.

    PTM databases

    PhosphoSiteiQ58F21.

    Expressioni

    Tissue specificityi

    Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expression is seen in patients' testes with abnormal spermatogenesis. Expressed in cancers such as non-small cell lung cancer and squamous cell carcinomas of the head and neck as well as of esophagus, but not in melanoma or in cancers of the colon, breast, kidney and bladder.3 Publications

    Developmental stagei

    Expressed in embryo testis.

    Gene expression databases

    ArrayExpressiQ58F21.
    BgeeiQ58F21.
    CleanExiHS_BRDT.
    GenevestigatoriQ58F21.

    Organism-specific databases

    HPAiCAB012237.
    HPA015283.

    Interactioni

    Subunit structurei

    Interacts with mRNA splicing machinery proteins SRSF2, DDX5, HNRNPK and TARDBP. Interacts with the acetylated N-terminus of histone H1, H2, H3 and H4. Interacts with P-TEFb components CDK9 and CCNT1/cyclin-T1. Interacts with SMARCE1 By similarity. Interacts with the acetylated N-terminus of histone H1.4, H2A, H2B, H3 and H4.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi107143. 4 interactions.
    IntActiQ58F21. 1 interaction.
    STRINGi9606.ENSP00000354568.

    Structurei

    Secondary structure

    1
    947
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 378
    Helixi39 – 446
    Helixi47 – 526
    Beta strandi53 – 553
    Turni58 – 625
    Helixi66 – 694
    Helixi76 – 849
    Helixi91 – 10818
    Helixi114 – 13017

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2RFJX-ray2.05A/B/C21-137[»]
    4FLPX-ray2.23A/B21-137[»]
    4KCXX-ray2.00A/B21-137[»]
    ProteinModelPortaliQ58F21.
    SMRiQ58F21. Positions 29-134, 266-377, 505-578.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ58F21.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 11673Bromo 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini287 – 35973Bromo 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini500 – 58283NETPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili417 – 47054Sequence AnalysisAdd
    BLAST
    Coiled coili591 – 62131Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi209 – 22012Nuclear localization signalAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi635 – 66733Ser-richAdd
    BLAST

    Domaini

    Bromo domains mediate interaction with histones that have acetylated lysine residues at specific positions (PubMed:22464331). Bromo domain 1 mediates binding with histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) (PubMed:22901802).2 Publications

    Sequence similaritiesi

    Contains 2 bromo domains.PROSITE-ProRule annotation
    Contains 1 NET domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Bromodomain, Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG5076.
    InParanoidiQ58F21.
    KOiK11724.
    OMAiENQRDLG.
    OrthoDBiEOG7TTQ86.
    PhylomeDBiQ58F21.
    TreeFamiTF317345.

    Family and domain databases

    Gene3Di1.20.920.10. 2 hits.
    InterProiIPR001487. Bromodomain.
    IPR018359. Bromodomain_CS.
    IPR027353. NET_dom.
    [Graphical view]
    PfamiPF00439. Bromodomain. 2 hits.
    [Graphical view]
    PRINTSiPR00503. BROMODOMAIN.
    SMARTiSM00297. BROMO. 2 hits.
    [Graphical view]
    SUPFAMiSSF47370. SSF47370. 2 hits.
    PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
    PS50014. BROMODOMAIN_2. 2 hits.
    PS51525. NET. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q58F21-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLPSRQTAI IVNPPPPEYI NTKKNGRLTN QLQYLQKVVL KDLWKHSFSW    50
    PFQRPVDAVK LQLPDYYTII KNPMDLNTIK KRLENKYYAK ASECIEDFNT 100
    MFSNCYLYNK PGDDIVLMAQ ALEKLFMQKL SQMPQEEQVV GVKERIKKGT 150
    QQNIAVSSAK EKSSPSATEK VFKQQEIPSV FPKTSISPLN VVQGASVNSS 200
    SQTAAQVTKG VKRKADTTTP ATSAVKASSE FSPTFTEKSV ALPPIKENMP 250
    KNVLPDSQQQ YNVVKTVKVT EQLRHCSEIL KEMLAKKHFS YAWPFYNPVD 300
    VNALGLHNYY DVVKNPMDLG TIKEKMDNQE YKDAYKFAAD VRLMFMNCYK 350
    YNPPDHEVVT MARMLQDVFE THFSKIPIEP VESMPLCYIK TDITETTGRE 400
    NTNEASSEGN SSDDSEDERV KRLAKLQEQL KAVHQQLQVL SQVPFRKLNK 450
    KKEKSKKEKK KEKVNNSNEN PRKMCEQMRL KEKSKRNQPK KRKQQFIGLK 500
    SEDEDNAKPM NYDEKRQLSL NINKLPGDKL GRVVHIIQSR EPSLSNSNPD 550
    EIEIDFETLK ASTLRELEKY VSACLRKRPL KPPAKKIMMS KEELHSQKKQ 600
    ELEKRLLDVN NQLNSRKRQT KSDKTQPSKA VENVSRLSES SSSSSSSSES 650
    ESSSSDLSSS DSSDSESEMF PKFTEVKPND SPSKENVKKM KNECIPPEGR 700
    TGVTQIGYCV QDTTSANTTL VHQTTPSHVM PPNHHQLAFN YQELEHLQTV 750
    KNISPLQILP PSGDSEQLSN GITVMHPSGD SDTTMLESEC QAPVQKDIKI 800
    KNADSWKSLG KPVKPSGVMK SSDELFNQFR KAAIEKEVKA RTQELIRKHL 850
    EQNTKELKAS QENQRDLGNG LTVESFSNKI QNKCSGEEQK EHQQSSEAQD 900
    KSKLWLLKDR DLARQKEQER RRREAMVGTI DMTLQSDIMT MFENNFD 947
    Length:947
    Mass (Da):107,954
    Last modified:April 14, 2009 - v4
    Checksum:i09C0FD8690E217BB
    GO
    Isoform 2 (identifier: Q58F21-2) [UniParc]FASTAAdd to Basket

    Also known as: BRDT-NY

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MTCTCRQDSKQLRM

    Show »
    Length:960
    Mass (Da):109,506
    Checksum:iD477F897F6D72935
    GO
    Isoform 3 (identifier: Q58F21-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         148-148: K → KGKAG

    Show »
    Length:951
    Mass (Da):108,268
    Checksum:i8A9508DF12C6AB36
    GO
    Isoform 4 (identifier: Q58F21-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         65-110: Missing.

    Show »
    Length:901
    Mass (Da):102,361
    Checksum:i64B19CBC80791B60
    GO
    Isoform 5 (identifier: Q58F21-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-73: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:874
    Mass (Da):99,394
    Checksum:iC8A7FAC55BA14F94
    GO

    Sequence cautioni

    The sequence AAH05281.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
    The sequence AAH47900.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
    The sequence AAH62700.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421D → N in BAH13876. (PubMed:14702039)Curated
    Sequence conflicti73 – 731P → S in AAH05281. (PubMed:15489334)Curated
    Sequence conflicti80 – 801K → I in AAH62700. (PubMed:15489334)Curated
    Sequence conflicti265 – 2651K → E in AAB87862. (PubMed:9367677)Curated
    Sequence conflicti336 – 3361K → S in AAB87862. (PubMed:9367677)Curated
    Sequence conflicti462 – 4621E → K in AAH17582. (PubMed:15489334)Curated
    Sequence conflicti477 – 4771Q → R in BAH13876. (PubMed:14702039)Curated
    Sequence conflicti552 – 5521I → M in AAQ16198. (PubMed:15647849)Curated
    Sequence conflicti686 – 6861N → H in AAB87862. (PubMed:9367677)Curated
    Sequence conflicti893 – 8931Q → P in AAB87862. (PubMed:9367677)Curated
    Sequence conflicti915 – 9151Q → P in AAB87862. (PubMed:9367677)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti2 – 21S → F.1 Publication
    Corresponds to variant rs55806733 [ dbSNP | Ensembl ].
    VAR_041924
    Natural varianti6 – 61R → Q.1 Publication
    Corresponds to variant rs56273490 [ dbSNP | Ensembl ].
    VAR_041925
    Natural varianti62 – 621Q → K.5 Publications
    Corresponds to variant rs10783071 [ dbSNP | Ensembl ].
    VAR_026584
    Natural varianti89 – 891A → V in a gastric adenocarcinoma sample; somatic mutation. 1 Publication
    VAR_041926
    Natural varianti238 – 2381K → N.2 Publications
    Corresponds to variant rs1156281 [ dbSNP | Ensembl ].
    VAR_026585
    Natural varianti288 – 2881H → Y in a lung neuroendocrine carcinoma sample; somatic mutation. 1 Publication
    VAR_041927
    Natural varianti336 – 3361K → T.
    Corresponds to variant rs1064567 [ dbSNP | Ensembl ].
    VAR_047327
    Natural varianti357 – 3571E → K.1 Publication
    Corresponds to variant rs34674879 [ dbSNP | Ensembl ].
    VAR_041928
    Natural varianti410 – 4101N → K.2 Publications
    Corresponds to variant rs3088232 [ dbSNP | Ensembl ].
    VAR_026586
    Natural varianti542 – 5421P → A.1 Publication
    Corresponds to variant rs55912588 [ dbSNP | Ensembl ].
    VAR_041929
    Natural varianti605 – 6051R → Q.
    Corresponds to variant rs35327986 [ dbSNP | Ensembl ].
    VAR_047328
    Natural varianti696 – 6961P → L.4 Publications
    Corresponds to variant rs10747493 [ dbSNP | Ensembl ].
    VAR_047329

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7373Missing in isoform 5. CuratedVSP_044509Add
    BLAST
    Alternative sequencei1 – 11M → MTCTCRQDSKQLRM in isoform 2. 1 PublicationVSP_019118
    Alternative sequencei65 – 11046Missing in isoform 4. 1 PublicationVSP_044510Add
    BLAST
    Alternative sequencei148 – 1481K → KGKAG in isoform 3. 1 PublicationVSP_044511

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019085 mRNA. Translation: AAB87862.1.
    AY338951 mRNA. Translation: AAQ16198.1.
    AK303008 mRNA. Translation: BAH13876.1.
    AK302758 mRNA. Translation: BAH13797.1.
    AK316442 mRNA. Translation: BAH14813.1.
    AC114486 Genomic DNA. No translation available.
    CH471097 Genomic DNA. Translation: EAW73106.1.
    CH471097 Genomic DNA. Translation: EAW73107.1.
    CH471097 Genomic DNA. Translation: EAW73108.1.
    CH471097 Genomic DNA. Translation: EAW73110.1.
    CH471097 Genomic DNA. Translation: EAW73111.1.
    BC005281 mRNA. Translation: AAH05281.1. Sequence problems.
    BC017582 mRNA. Translation: AAH17582.1.
    BC047900 mRNA. Translation: AAH47900.1. Sequence problems.
    BC062700 mRNA. Translation: AAH62700.1. Sequence problems.
    CCDSiCCDS55615.1. [Q58F21-4]
    CCDS55616.1. [Q58F21-5]
    CCDS735.1. [Q58F21-1]
    RefSeqiNP_001229734.1. NM_001242805.1.
    NP_001229735.1. NM_001242806.1.
    NP_001229736.1. NM_001242807.1.
    NP_001229737.1. NM_001242808.1.
    NP_001229739.1. NM_001242810.1.
    NP_001717.2. NM_001726.3.
    NP_997072.1. NM_207189.2.
    XP_005271208.1. XM_005271151.2. [Q58F21-1]
    XP_006710916.1. XM_006710853.1. [Q58F21-1]
    XP_006710917.1. XM_006710854.1. [Q58F21-1]
    XP_006710918.1. XM_006710855.1. [Q58F21-1]
    XP_006710919.1. XM_006710856.1. [Q58F21-1]
    XP_006710920.1. XM_006710857.1. [Q58F21-1]
    UniGeneiHs.482520.

    Genome annotation databases

    EnsembliENST00000362005; ENSP00000354568; ENSG00000137948. [Q58F21-1]
    ENST00000370389; ENSP00000359416; ENSG00000137948. [Q58F21-5]
    ENST00000394530; ENSP00000378038; ENSG00000137948. [Q58F21-4]
    ENST00000399546; ENSP00000387822; ENSG00000137948. [Q58F21-1]
    ENST00000402388; ENSP00000384051; ENSG00000137948. [Q58F21-1]
    GeneIDi676.
    KEGGihsa:676.
    UCSCiuc001dok.4. human. [Q58F21-1]
    uc010osz.2. human. [Q58F21-3]

    Polymorphism databases

    DMDMi226694198.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Asking life to be patient - Issue 144 of November 2012

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019085 mRNA. Translation: AAB87862.1 .
    AY338951 mRNA. Translation: AAQ16198.1 .
    AK303008 mRNA. Translation: BAH13876.1 .
    AK302758 mRNA. Translation: BAH13797.1 .
    AK316442 mRNA. Translation: BAH14813.1 .
    AC114486 Genomic DNA. No translation available.
    CH471097 Genomic DNA. Translation: EAW73106.1 .
    CH471097 Genomic DNA. Translation: EAW73107.1 .
    CH471097 Genomic DNA. Translation: EAW73108.1 .
    CH471097 Genomic DNA. Translation: EAW73110.1 .
    CH471097 Genomic DNA. Translation: EAW73111.1 .
    BC005281 mRNA. Translation: AAH05281.1 . Sequence problems.
    BC017582 mRNA. Translation: AAH17582.1 .
    BC047900 mRNA. Translation: AAH47900.1 . Sequence problems.
    BC062700 mRNA. Translation: AAH62700.1 . Sequence problems.
    CCDSi CCDS55615.1. [Q58F21-4 ]
    CCDS55616.1. [Q58F21-5 ]
    CCDS735.1. [Q58F21-1 ]
    RefSeqi NP_001229734.1. NM_001242805.1.
    NP_001229735.1. NM_001242806.1.
    NP_001229736.1. NM_001242807.1.
    NP_001229737.1. NM_001242808.1.
    NP_001229739.1. NM_001242810.1.
    NP_001717.2. NM_001726.3.
    NP_997072.1. NM_207189.2.
    XP_005271208.1. XM_005271151.2. [Q58F21-1 ]
    XP_006710916.1. XM_006710853.1. [Q58F21-1 ]
    XP_006710917.1. XM_006710854.1. [Q58F21-1 ]
    XP_006710918.1. XM_006710855.1. [Q58F21-1 ]
    XP_006710919.1. XM_006710856.1. [Q58F21-1 ]
    XP_006710920.1. XM_006710857.1. [Q58F21-1 ]
    UniGenei Hs.482520.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2RFJ X-ray 2.05 A/B/C 21-137 [» ]
    4FLP X-ray 2.23 A/B 21-137 [» ]
    4KCX X-ray 2.00 A/B 21-137 [» ]
    ProteinModelPortali Q58F21.
    SMRi Q58F21. Positions 29-134, 266-377, 505-578.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107143. 4 interactions.
    IntActi Q58F21. 1 interaction.
    STRINGi 9606.ENSP00000354568.

    Chemistry

    ChEMBLi CHEMBL1795185.
    GuidetoPHARMACOLOGYi 2729.

    PTM databases

    PhosphoSitei Q58F21.

    Polymorphism databases

    DMDMi 226694198.

    Proteomic databases

    MaxQBi Q58F21.
    PaxDbi Q58F21.
    PRIDEi Q58F21.

    Protocols and materials databases

    DNASUi 676.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000362005 ; ENSP00000354568 ; ENSG00000137948 . [Q58F21-1 ]
    ENST00000370389 ; ENSP00000359416 ; ENSG00000137948 . [Q58F21-5 ]
    ENST00000394530 ; ENSP00000378038 ; ENSG00000137948 . [Q58F21-4 ]
    ENST00000399546 ; ENSP00000387822 ; ENSG00000137948 . [Q58F21-1 ]
    ENST00000402388 ; ENSP00000384051 ; ENSG00000137948 . [Q58F21-1 ]
    GeneIDi 676.
    KEGGi hsa:676.
    UCSCi uc001dok.4. human. [Q58F21-1 ]
    uc010osz.2. human. [Q58F21-3 ]

    Organism-specific databases

    CTDi 676.
    GeneCardsi GC01P092414.
    HGNCi HGNC:1105. BRDT.
    HPAi CAB012237.
    HPA015283.
    MIMi 602144. gene.
    neXtProti NX_Q58F21.
    PharmGKBi PA25418.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5076.
    InParanoidi Q58F21.
    KOi K11724.
    OMAi ENQRDLG.
    OrthoDBi EOG7TTQ86.
    PhylomeDBi Q58F21.
    TreeFami TF317345.

    Miscellaneous databases

    ChiTaRSi BRDT. human.
    EvolutionaryTracei Q58F21.
    GeneWikii BRDT.
    GenomeRNAii 676.
    NextBioi 2784.
    PROi Q58F21.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q58F21.
    Bgeei Q58F21.
    CleanExi HS_BRDT.
    Genevestigatori Q58F21.

    Family and domain databases

    Gene3Di 1.20.920.10. 2 hits.
    InterProi IPR001487. Bromodomain.
    IPR018359. Bromodomain_CS.
    IPR027353. NET_dom.
    [Graphical view ]
    Pfami PF00439. Bromodomain. 2 hits.
    [Graphical view ]
    PRINTSi PR00503. BROMODOMAIN.
    SMARTi SM00297. BROMO. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47370. SSF47370. 2 hits.
    PROSITEi PS00633. BROMODOMAIN_1. 2 hits.
    PS50014. BROMODOMAIN_2. 2 hits.
    PS51525. NET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of BRDT: a testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh."
      Jones M.H., Numata M., Shimane M.
      Genomics 45:529-534(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANTS LYS-62 AND LEU-696.
    2. "Molecular cloning and expression of a novel alternative splice variant of BRDT gene."
      Zheng Y., Yuan W., Zhou Z., Xu M., Sha J.-H.
      Int. J. Mol. Med. 15:315-321(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, VARIANTS LYS-62 AND LEU-696.
      Tissue: Testis.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), VARIANTS LYS-62 AND LEU-696.
      Tissue: Testis.
    4. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS LYS-62 AND LEU-696.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-462 (ISOFORM 1), VARIANTS LYS-62; ASN-238 AND LYS-410.
      Tissue: Brain and Testis.
    7. "Expression of cancer-testis antigens in lung cancer: definition of bromodomain testis-specific gene (BRDT) as a new CT gene, CT9."
      Scanlan M.J., Altorki N.K., Gure A.O., Williamson B., Jungbluth A., Chen Y.-T., Old L.J.
      Cancer Lett. 150:155-164(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
    8. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] PHE-2; GLN-6; VAL-89; ASN-238; TYR-288; LYS-357; LYS-410 AND ALA-542.
    9. "The interaction of modified histones with the bromodomain testis-specific (BRDT) gene and its mRNA level in sperm of fertile donors and subfertile men."
      Steilmann C., Cavalcanti M.C., Bartkuhn M., Pons-Kuhnemann J., Schuppe H.C., Weidner W., Steger K., Paradowska A.
      Reproduction 140:435-443(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "The conserved 12-amino acid stretch in the inter-bromodomain region of BET family proteins functions as a nuclear localization signal."
      Fukazawa H., Masumi A.
      Biol. Pharm. Bull. 35:2064-2068(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MOTIF NUCLEAR LOCALIZATION SIGNAL.
    11. Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 21-137, SUBUNIT, DOMAIN.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (2.23 ANGSTROMS) OF 21-137 IN COMPLEX WITH JQ1, SUBCELLULAR LOCATION, FUNCTION.

    Entry informationi

    Entry nameiBRDT_HUMAN
    AccessioniPrimary (citable) accession number: Q58F21
    Secondary accession number(s): A6NF68
    , B7Z811, B7Z890, B7ZAX7, D3DT32, O14789, Q05DQ4, Q6P5T1, Q7Z4A6, Q8IWI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 91 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    BRDT is a promising target for male contraception. Inhibition by thienodiazepine inhibitor (+)-JQ1 that binds Asn-109, prevents recognition of acetylated histone H4, causing a complete and reversible contraceptive effect in male mice (PubMed:22901802).1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3