Q58EK5 (TDRD1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tudor domain-containing protein 1

Gene names

Name:	tdrd1
ORF Names:	im:7155161

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	1175 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a central role during spermatogenesis by participating in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the localization of Piwi proteins to the meiotic nuage. Involved in the piRNA metabolic process by ensuring the entry of correct transcripts into the normal piRNA pool and limiting the entry of cellular transcripts into the piRNA pathway. May act by allowing the recruitment of piRNA biogenesis or loading factors that ensure the correct entry of transcripts and piRNAs into Piwi proteins By similarity.
Subcellular location	Cytoplasm By similarity. Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis By similarity.
Sequence similarities	Belongs to the TDRD1 family. Contains 1 MYND-type zinc finger. Contains 4 Tudor domains.

Ontologies

Keywords
Biological process	Differentiation Meiosis RNA-mediated gene silencing
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA methylation involved in gamete generation Inferred from sequence or structural similarity. Source: UniProtKB gene silencing by RNA Inferred from sequence or structural similarity. Source: UniProtKB germ cell development Inferred from sequence or structural similarity. Source: UniProtKB meiosis Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW piRNA metabolic process Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	pi-body Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1175	1175	Tudor domain-containing protein 1		PRO_0000367315
Regions
	Domain	222 – 282	61	Tudor 1
	Domain	453 – 512	60	Tudor 2
	Domain	674 – 733	60	Tudor 3
	Domain	925 – 983	59	Tudor 4
	Zinc finger	83 – 119	37	MYND-type
Experimental info
	Sequence conflict	691	1	W → G in AAH91863. Ref.2
	Sequence conflict	748	1	V → M in AAH91863. Ref.2
	Sequence conflict	836	1	P → Q in AAH91863. Ref.2
	Sequence conflict	1132	1	S → P in AAH91863. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q58EK5 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 6DD18C1D98CAB859	FASTA	1,175	129,042
	10 20 30 40 50 60 MNPAFAQPMM RPNLPLRRPA TGPSSLSPRG PAPAIYEERL LASDVLDGPK IDTMRKDISQ 70 80 90 100 110 120 NCGDVLSSPQ TAVSMMGQVV KLCNYCSHQG NLRCTRCKKT CYCSVACQTQ DWIAHRHVCK 130 140 150 160 170 180 PSIPEVTSEK PKESKAVPYA NGLGGTQAKE ISVDAQPKRI YRRDLHKKVV SKGSEIKGTV 190 200 210 220 230 240 IDLRNPGMFS IHCQCEEMIE SLKKITQQLQ KTYCSSFAQE YKPEVGELCA VKFSLDQNWY 250 260 270 280 290 300 RAEIQAVDVA RKTAGVFYID FGNEENVALD HIRPLSENID AVPPFALQCC IAGVKPLTGS 310 320 330 340 350 360 WTGECCIAVR QLIAGKSLTF TVLDIMNDGD LLAVDSLVST LGKHVSTFLI DQSYAIKEDV 370 380 390 400 410 420 PVKTQTEHSI SSLLTASFEN FKRFSGGKNE NSEARPPEPL TQGVGDSFTA VVTHLQSPSE 430 440 450 460 470 480 ILCQKLENAS IIQQLQMNLR VHCSNTAASD DFRPAPGTVC CSLFSEDNQW YRAKVLAYSS 490 500 510 520 530 540 EDRVCVGYID FGNSEEVELN RLRPISKELL ALATQAIPCS LAGIKSLTDT WSDEAVLMLK 550 560 570 580 590 600 HLVCNRFIRV EILGKKDGRA LVSMIDESSD PQASVTELLV NMGFAAIESV ETKKNEPDPA 610 620 630 640 650 660 TSTEIPLSQP VVEKLEWTGA ELPFDGQKVE LVISTLKSLD EFYCYNYSKT DEHTLTEMSF 670 680 690 700 710 720 ELMKHCESER APFTPIVGEP CCALFTGDAR WYRAMVLEVC GEGKARVCFV DYGNSCEVDA 730 740 750 760 770 780 AHLKAITQSL LKLPFQAIRC WLAGVEPVEG QWKKEAMLRF QALCAGQPLS GKVLSITEKG 790 800 810 820 830 840 YGMELESAGQ TVASVLISEH LAKPYGQVRQ PPQIQPAKPA SQIEDLPSLK PIDQNPSVEE 850 860 870 880 890 900 PLKVSSKGAA TTPEDLPVSS GCFPLNWKTL ELSCSGTFQP RVAAVISPSL FYIMNPGQVN 910 920 930 940 950 960 VEGLKAVMTD VAKYCSKQPV PNQCHPLPGA SCCAQFSGDK NWYRAVVLEV TTKHAHVIYS 970 980 990 1000 1010 1020 DYGNMETVPL SSILPITKEL LQHPFQIVRC ALTGKEHFPV VWPTEVLELF GIQLSGGVLA 1030 1040 1050 1060 1070 1080 SFQGFDGTSN LLTLTQQSGQ SDRDINSIIL GALQKGQIKP SSKLPANVNE EKKDVEQKQT 1090 1100 1110 1120 1130 1140 QPISSNKAVE QTLSTNVEKP ALDDQTLPLS VSLKHEEPEN MSKTKTAEEC TSTPDTVSSI 1150 1160 1170 ESSHAAQSCC CQELKQKMDR IEELVLLLVK QVGSR

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 691-1175. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR932360 Genomic DNA. No translation available. BC091863 mRNA. Translation: AAH91863.1.
IPI	IPI00481706.
UniGene	Dr.133744.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-070803-1. tdrd1.
Phylogenomic databases
eggNOG	fiNOG15419.
GeneTree	ENSGT00520000055580.
HOVERGEN	HBG108556.
InParanoid	Q58EK5.
OrthoDB	EOG4C87RJ.
Gene expression databases
Bgee	Q58EK5.
Family and domain databases
InterPro	IPR008191. Maternal_tudor. IPR002999. Tudor. IPR018351. Tudor_subgr. IPR002893. Znf_MYND. [Graphical view]
Pfam	PF00567. TUDOR. 4 hits. PF01753. zf-MYND. 1 hit. [Graphical view]
SMART	SM00333. TUDOR. 4 hits. [Graphical view]
PROSITE	PS50304. TUDOR. 4 hits. PS01360. ZF_MYND_1. 1 hit. PS50865. ZF_MYND_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDRD1_DANRE

Accession

Primary (citable) accession number: Q58EK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program