Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cystathionine gamma-lyase

Gene

CTH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function (By similarity).By similarity

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cystathionine gamma-lyase (CTH)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621SubstrateBy similarity
Binding sitei114 – 1141SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei339 – 3391SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Calmodulin-binding, Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00136; UER00202.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Cysteine-protein sulfhydrase
Gamma-cystathionase
Gene namesi
Name:CTH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Cystathionine gamma-lyasePRO_0000245576Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei212 – 2121N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ58DW2.
PRIDEiQ58DW2.

Interactioni

Subunit structurei

Homotetramer. Interacts with CALM in a calcium-dependent manner (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000056275.

Structurei

3D structure databases

ProteinModelPortaliQ58DW2.
SMRiQ58DW2. Positions 10-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
HOVERGENiHBG005322.
InParanoidiQ58DW2.
KOiK01758.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58DW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEKEASPHG FLPRFQHFAT QAIHVGQEPE QWTSQAVVPP ISLSTTFKQG
60 70 80 90 100
APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYSLAFASG LAATVTITHL
110 120 130 140 150
LKAGDQIICM DDVYGGTNRY FRQVATEFGL KISFVDCSKP KLLEAAITPE
160 170 180 190 200
TKLVWIETPT NPSLKMIDIE ACAHTVHKHG DIILVVDNTF MSAYFQRPLS
210 220 230 240 250
LGADICMYSA TKYMNGYSDV VMGLVSLNSE SLHDRLRFLQ NSLGAVPSPI
260 270 280 290 300
DCYLCNRGLK TLQVRMEKHF ENGMAVAQFL ESNPQVEKVI YPGLPSHPQH
310 320 330 340 350
ELAKRQCTGC PGMVTFYIKG SLQHAETFLQ NLKLFTLAES LGGYESLAEL
360 370 380 390 400
PAIMTHASVP KSDREVLGIS DTLIRLSVGL EDKQDLLDDL DQALKAANPP

NASSN
Length:405
Mass (Da):44,406
Last modified:April 26, 2005 - v1
Checksum:i53B1E2AF2A6C194A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021485 mRNA. Translation: AAX46332.1.
RefSeqiNP_001019738.1. NM_001024567.1.
UniGeneiBt.37399.
Bt.73096.

Genome annotation databases

GeneIDi539159.
KEGGibta:539159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021485 mRNA. Translation: AAX46332.1.
RefSeqiNP_001019738.1. NM_001024567.1.
UniGeneiBt.37399.
Bt.73096.

3D structure databases

ProteinModelPortaliQ58DW2.
SMRiQ58DW2. Positions 10-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000056275.

Proteomic databases

PaxDbiQ58DW2.
PRIDEiQ58DW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi539159.
KEGGibta:539159.

Organism-specific databases

CTDi1491.

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
HOVERGENiHBG005322.
InParanoidiQ58DW2.
KOiK01758.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00202.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiCGL_BOVIN
AccessioniPrimary (citable) accession number: Q58DW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: April 26, 2005
Last modified: June 8, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.