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Protein

Pyrroline-5-carboxylate reductase 1, mitochondrial

Gene

PYCR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress (By similarity).By similarity

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrroline-5-carboxylate reductase 2 (PYCR2), Pyrroline-5-carboxylate reductase 3 (PYCRL), Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADP; via carbonyl oxygenBy similarity
Binding sitei56 – 561NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 116NADPBy similarity
Nucleotide bindingi69 – 724NADPBy similarity
Nucleotide bindingi95 – 973NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis, Stress response

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-BTA-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase 1, mitochondrial (EC:1.5.1.2)
Short name:
P5C reductase 1
Short name:
P5CR 1
Gene namesi
Name:PYCR1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 320319Pyrroline-5-carboxylate reductase 1, mitochondrialPRO_0000270817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei278 – 2781PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ58DT4.
PeptideAtlasiQ58DT4.
PRIDEiQ58DT4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000000042.

Interactioni

Subunit structurei

Homodecamer; composed of 5 homodimers.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000046.

Structurei

3D structure databases

ProteinModelPortaliQ58DT4.
SMRiQ58DT4. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3124. Eukaryota.
COG0345. LUCA.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ58DT4.
KOiK00286.
OMAiQPIARTM.
OrthoDBiEOG091G0KMI.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58DT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVGFIGAGQ LAFALAKGFT AAGVVAAHKI MASSPDMDLA TVSALRKMGV
60 70 80 90 100
NLTHHNKETV QHSDVLFLAV KPHIIPFILD EIAANIEARH IVVSCAAGVT
110 120 130 140 150
ISSIEKKLTA FQPAPKVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLLE
160 170 180 190 200
QLMSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR
210 220 230 240 250
LAVRLGAQAL LGAAKMLLDS EQHPGQLKDN VCSPGGATIH ALHVLESGGF
260 270 280 290 300
RSLLIKAVEA SCTRTRELQS MADQEKVSPA AIKKTILDKV KLDSPPGTSL
310 320
APSGHSKLLP RSMAPAGKQD
Length:320
Mass (Da):33,454
Last modified:April 26, 2005 - v1
Checksum:i0265F89F283CA765
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021513 mRNA. Translation: AAX46360.1.
BC123510 mRNA. Translation: AAI23511.1.
RefSeqiNP_001014957.1. NM_001014957.1.
XP_010814772.1. XM_010816470.1.
UniGeneiBt.12489.

Genome annotation databases

EnsembliENSBTAT00000000046; ENSBTAP00000000046; ENSBTAG00000000042.
GeneIDi539606.
KEGGibta:539606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021513 mRNA. Translation: AAX46360.1.
BC123510 mRNA. Translation: AAI23511.1.
RefSeqiNP_001014957.1. NM_001014957.1.
XP_010814772.1. XM_010816470.1.
UniGeneiBt.12489.

3D structure databases

ProteinModelPortaliQ58DT4.
SMRiQ58DT4. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000046.

Proteomic databases

PaxDbiQ58DT4.
PeptideAtlasiQ58DT4.
PRIDEiQ58DT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000046; ENSBTAP00000000046; ENSBTAG00000000042.
GeneIDi539606.
KEGGibta:539606.

Organism-specific databases

CTDi5831.

Phylogenomic databases

eggNOGiKOG3124. Eukaryota.
COG0345. LUCA.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ58DT4.
KOiK00286.
OMAiQPIARTM.
OrthoDBiEOG091G0KMI.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.
ReactomeiR-BTA-70614. Amino acid synthesis and interconversion (transamination).

Gene expression databases

BgeeiENSBTAG00000000042.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP5CR1_BOVIN
AccessioniPrimary (citable) accession number: Q58DT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: April 26, 2005
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.