Reviewed, UniProtKB/Swiss-Prot Q58DS7 (PON2_BOVIN)

Names and origin

Protein names

Recommended name:
    Serum paraoxonase/arylesterase 2
      Short name=PON 2
    EC=3.1.1.2
    EC=3.1.8.1
Alternative name(s):
    Serum aryldialkylphosphatase 2
    Aromatic esterase 2
      Short name=A-esterase 2

Gene names

Name:

PON2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters By similarity.
Catalytic activity	A phenyl acetate + H2O = a phenol + acetate. An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Glycosylated By similarity. The signal sequence is not cleaved By similarity.
Sequence similarities	Belongs to the paraoxonase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aryldialkylphosphatase activity Inferred from electronic annotation. Source: EC arylesterase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Serum paraoxonase/arylesterase 2		PRO_0000223285
	Signal peptide	1 – ?		Not cleaved Potential
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	53	1	Calcium 1; catalytic By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	116	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	167	1	Calcium 1; catalytic By similarity
	Metal binding	168	1	Calcium 2 By similarity
	Metal binding	223	1	Calcium 1; catalytic By similarity
	Metal binding	268	1	Calcium 1; catalytic By similarity
	Metal binding	269	1	Calcium 1; catalytic By similarity
Amino acid modifications
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 352		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58DS7-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: BB49FA5EC3170239	FASTA	354	39,421
	10 20 30 40 50 60 MGRLLALSLL GIALALLGER LLALRNRLKA SREVESVDLP NCHLIKGIEA GAEDIDILPN 70 80 90 100 110 120 GLAFFSVGLK CPGLHSFAPD KPGGILMMDL NEENPRALEL RVSRGFNLAS FNPHGISTFI 130 140 150 160 170 180 DSDDTVYLFV VNHPEFKNTV EIFKFEEEEN SLLHLKTIKH ELLPSVNDII AVGPEHFYAT 190 200 210 220 230 240 NDHYFSDPFL KYLETYLNLH WTNVVYYSPN EVKVVAEGFD SANGINISPD KKYIYVADIL 250 260 270 280 290 300 AHEIHVLEKH PNMNLTQLKV LKLDTLVDNL SIDPSSGDVL VGCHPNGQKL FVYDPKNPPS 310 320 330 340 350 SEVLRIQNIL SEKPTVTTVY ANNGSVLQGS SVASVYDKKL LIGTLYHRAL YCEL

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT021520 mRNA. Translation: AAX46367.1. BC118371 mRNA. Translation: AAI18372.1.
IPI	IPI00695295.
RefSeq	NP_001013606.1.
UniGene	Bt.23599
3D structure databases
SMR	Q58DS7. Positions 23-354.
ModBase	Search...
Protein-protein interaction databases
STRING	Q58DS7.
Genome annotation databases
Ensembl	ENSBTAT00000011009; ENSBTAP00000011009; ENSBTAG00000008361; Bos taurus. [Genome view]
GeneID	281417.
KEGG	bta:281417.
Organism-specific databases
CTD	281417.
Phylogenomic databases
eggNOG	maNOG17971.
HOVERGEN	Q58DS7.
InParanoid	Q58DS7.
PhylomeDB	Q58DS7.
Enzyme and pathway databases
BRENDA	3.1.1.2. 251. 3.1.8.1. 251.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR002640. Arylesterase. IPR008364. Paraoxonase2. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF01731. Arylesterase. 1 hit. [Graphical view]
PRINTS	PR01785. PARAOXONASE. PR01787. PARAOXONASE2.
ProtoNet	Search...

Entry information

Entry name

PON2_BOVIN

Accession

Primary (citable) accession number: Q58DS7
Secondary accession number(s): Q148F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	April 26, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)