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Protein

Enoyl-CoA hydratase, mitochondrial

Gene

ECHS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate.By similarity

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
Sitei164 – 1641Important for catalytic activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11697.
ReactomeiR-BTA-77310. Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA.
R-BTA-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
R-BTA-77348. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
R-BTA-77350. Beta oxidation of hexanoyl-CoA to butanoyl-CoA.
R-BTA-77352. Beta oxidation of butanoyl-CoA to acetyl-CoA.
SABIO-RKQ58DM8.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-CoA hydratase, mitochondrial (EC:4.2.1.17)
Alternative name(s):
Enoyl-CoA hydratase 1
Short-chain enoyl-CoA hydratase
Short name:
SCEH
Gene namesi
Name:ECHS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 290263Enoyl-CoA hydratase, mitochondrialPRO_0000007410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei101 – 1011N6-acetyllysine; alternateBy similarity
Modified residuei101 – 1011N6-succinyllysine; alternateBy similarity
Modified residuei204 – 2041N6-succinyllysineBy similarity
Modified residuei211 – 2111N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ58DM8.
PRIDEiQ58DM8.

Interactioni

Subunit structurei

Homohexamer; dimer of trimers.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042386.

Structurei

3D structure databases

ProteinModelPortaliQ58DM8.
SMRiQ58DM8. Positions 31-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1014Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1680. Eukaryota.
COG1024. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG010157.
InParanoidiQ58DM8.
KOiK07511.
OMAiSKAMEMV.
OrthoDBiEOG7N63NC.
TreeFamiTF314497.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58DM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRALLPR VRAPLRPWLF CPVQRSFASS AAFEYIITAK KGRNSNVGLI
60 70 80 90 100
QLNRPKALNA LCNGLIVELN QALQAFEEDP AVGAIVLTGG EKVFAAGADI
110 120 130 140 150
KEMQSLTFQN CYSGGFLSHW DQLTRVKKPV IAAVNGYALG GGCELAMMCD
160 170 180 190 200
IIYAGEKAQF GQPEILIGTI PGAGGTQRLT RAVGKSLAME MVLTGDRISA
210 220 230 240 250
QDAKQAGLVS KIFPVETVVE EAIQCAEKIA SNSKIVTAMA KESVNAAFEM
260 270 280 290
TLAEGVKLEK KLFYSTFATE DRKEGMAAFV EKRKANFKDQ
Length:290
Mass (Da):31,243
Last modified:April 26, 2005 - v1
Checksum:i30CAD9F7314AD50F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981I → N in AAI09606 (Ref. 3) Curated
Sequence conflicti269 – 2779TEDRKEGMA → PKTGRKAWP in AAY83884 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ058610 mRNA. Translation: AAY83884.1.
DQ058603 Genomic DNA. Translation: AAY83878.1.
BT021569 mRNA. Translation: AAX46416.1.
BC109605 mRNA. Translation: AAI09606.1.
RefSeqiNP_001020377.2. NM_001025206.2.
UniGeneiBt.64629.

Genome annotation databases

EnsembliENSBTAT00000044947; ENSBTAP00000042386; ENSBTAG00000017710.
GeneIDi281748.
KEGGibta:281748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ058610 mRNA. Translation: AAY83884.1.
DQ058603 Genomic DNA. Translation: AAY83878.1.
BT021569 mRNA. Translation: AAX46416.1.
BC109605 mRNA. Translation: AAI09606.1.
RefSeqiNP_001020377.2. NM_001025206.2.
UniGeneiBt.64629.

3D structure databases

ProteinModelPortaliQ58DM8.
SMRiQ58DM8. Positions 31-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042386.

Proteomic databases

PaxDbiQ58DM8.
PRIDEiQ58DM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000044947; ENSBTAP00000042386; ENSBTAG00000017710.
GeneIDi281748.
KEGGibta:281748.

Organism-specific databases

CTDi1892.

Phylogenomic databases

eggNOGiKOG1680. Eukaryota.
COG1024. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG010157.
InParanoidiQ58DM8.
KOiK07511.
OMAiSKAMEMV.
OrthoDBiEOG7N63NC.
TreeFamiTF314497.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciMetaCyc:MONOMER-11697.
ReactomeiR-BTA-77310. Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA.
R-BTA-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
R-BTA-77348. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
R-BTA-77350. Beta oxidation of hexanoyl-CoA to butanoyl-CoA.
R-BTA-77352. Beta oxidation of butanoyl-CoA to acetyl-CoA.
SABIO-RKQ58DM8.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative mapping of the bovine SPRN locus."
    Ferretti L., Uboldi C., Del Vecchio I., Eggen A., Brunner R., Iannuzzi L.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiECHM_BOVIN
AccessioniPrimary (citable) accession number: Q58DM8
Secondary accession number(s): Q2TBV2, Q4PS76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: April 26, 2005
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.