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Protein

Enoyl-CoA hydratase, mitochondrial

Gene

ECHS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate (By similarity). Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate (By similarity).By similarity

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.By similarity

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141Substrate; via amide nitrogenBy similarity1
Sitei164Important for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11697
ReactomeiR-BTA-77310 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
R-BTA-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
R-BTA-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
R-BTA-77350 Beta oxidation of hexanoyl-CoA to butanoyl-CoA
R-BTA-77352 Beta oxidation of butanoyl-CoA to acetyl-CoA
SABIO-RKiQ58DM8
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-CoA hydratase, mitochondrial (EC:4.2.1.17By similarity)
Alternative name(s):
Enoyl-CoA hydratase 1
Short-chain enoyl-CoA hydratase
Short name:
SCEH
Gene namesi
Name:ECHS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Organism-specific databases

VGNCiVGNC:28308 ECHS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000000741028 – 290Enoyl-CoA hydratase, mitochondrialAdd BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101N6-acetyllysine; alternateBy similarity1
Modified residuei101N6-succinyllysine; alternateBy similarity1
Modified residuei204N6-succinyllysineBy similarity1
Modified residuei211N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ58DM8
PeptideAtlasiQ58DM8
PRIDEiQ58DM8

Expressioni

Gene expression databases

BgeeiENSBTAG00000017710

Interactioni

Subunit structurei

Homohexamer; dimer of trimers.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042386

Structurei

3D structure databases

ProteinModelPortaliQ58DM8
SMRiQ58DM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 101Substrate bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1680 Eukaryota
COG1024 LUCA
GeneTreeiENSGT00890000139344
HOGENOMiHOG000027939
HOVERGENiHBG010157
InParanoidiQ58DM8
KOiK07511
OMAiIFKQTDA
OrthoDBiEOG091G0MVQ
TreeFamiTF314497

Family and domain databases

Gene3Di1.10.12.10, 1 hit
InterProiView protein in InterPro
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR014748 enoyl-CoA_hydra_C
PfamiView protein in Pfam
PF00378 ECH_1, 1 hit
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58DM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRALLPR VRAPLRPWLF CPVQRSFASS AAFEYIITAK KGRNSNVGLI
60 70 80 90 100
QLNRPKALNA LCNGLIVELN QALQAFEEDP AVGAIVLTGG EKVFAAGADI
110 120 130 140 150
KEMQSLTFQN CYSGGFLSHW DQLTRVKKPV IAAVNGYALG GGCELAMMCD
160 170 180 190 200
IIYAGEKAQF GQPEILIGTI PGAGGTQRLT RAVGKSLAME MVLTGDRISA
210 220 230 240 250
QDAKQAGLVS KIFPVETVVE EAIQCAEKIA SNSKIVTAMA KESVNAAFEM
260 270 280 290
TLAEGVKLEK KLFYSTFATE DRKEGMAAFV EKRKANFKDQ
Length:290
Mass (Da):31,243
Last modified:April 26, 2005 - v1
Checksum:i30CAD9F7314AD50F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198I → N in AAI09606 (Ref. 3) Curated1
Sequence conflicti269 – 277TEDRKEGMA → PKTGRKAWP in AAY83884 (Ref. 1) Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ058610 mRNA Translation: AAY83884.1
DQ058603 Genomic DNA Translation: AAY83878.1
BT021569 mRNA Translation: AAX46416.1
BC109605 mRNA Translation: AAI09606.1
RefSeqiNP_001020377.2, NM_001025206.2
UniGeneiBt.64629

Genome annotation databases

EnsembliENSBTAT00000044947; ENSBTAP00000042386; ENSBTAG00000017710
GeneIDi281748
KEGGibta:281748

Similar proteinsi

Entry informationi

Entry nameiECHM_BOVIN
AccessioniPrimary (citable) accession number: Q58DM8
Secondary accession number(s): Q2TBV2, Q4PS76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: April 26, 2005
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health