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Protein

Enoyl-CoA hydratase, mitochondrial

Gene

ECHS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate (By similarity). Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate (By similarity).By similarity

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.By similarity

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141Substrate; via amide nitrogenBy similarity1
Sitei164Important for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11697.
ReactomeiR-BTA-77310. Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA.
R-BTA-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
R-BTA-77348. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
R-BTA-77350. Beta oxidation of hexanoyl-CoA to butanoyl-CoA.
R-BTA-77352. Beta oxidation of butanoyl-CoA to acetyl-CoA.
SABIO-RKQ58DM8.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-CoA hydratase, mitochondrial (EC:4.2.1.17By similarity)
Alternative name(s):
Enoyl-CoA hydratase 1
Short-chain enoyl-CoA hydratase
Short name:
SCEH
Gene namesi
Name:ECHS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000000741028 – 290Enoyl-CoA hydratase, mitochondrialAdd BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101N6-acetyllysine; alternateBy similarity1
Modified residuei101N6-succinyllysine; alternateBy similarity1
Modified residuei204N6-succinyllysineBy similarity1
Modified residuei211N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ58DM8.
PeptideAtlasiQ58DM8.
PRIDEiQ58DM8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017710.

Interactioni

Subunit structurei

Homohexamer; dimer of trimers.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042386.

Structurei

3D structure databases

ProteinModelPortaliQ58DM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 101Substrate bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1680. Eukaryota.
COG1024. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG010157.
InParanoidiQ58DM8.
KOiK07511.
OMAiVSKIYPV.
OrthoDBiEOG091G0MVQ.
TreeFamiTF314497.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58DM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRALLPR VRAPLRPWLF CPVQRSFASS AAFEYIITAK KGRNSNVGLI
60 70 80 90 100
QLNRPKALNA LCNGLIVELN QALQAFEEDP AVGAIVLTGG EKVFAAGADI
110 120 130 140 150
KEMQSLTFQN CYSGGFLSHW DQLTRVKKPV IAAVNGYALG GGCELAMMCD
160 170 180 190 200
IIYAGEKAQF GQPEILIGTI PGAGGTQRLT RAVGKSLAME MVLTGDRISA
210 220 230 240 250
QDAKQAGLVS KIFPVETVVE EAIQCAEKIA SNSKIVTAMA KESVNAAFEM
260 270 280 290
TLAEGVKLEK KLFYSTFATE DRKEGMAAFV EKRKANFKDQ
Length:290
Mass (Da):31,243
Last modified:April 26, 2005 - v1
Checksum:i30CAD9F7314AD50F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198I → N in AAI09606 (Ref. 3) Curated1
Sequence conflicti269 – 277TEDRKEGMA → PKTGRKAWP in AAY83884 (Ref. 1) Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ058610 mRNA. Translation: AAY83884.1.
DQ058603 Genomic DNA. Translation: AAY83878.1.
BT021569 mRNA. Translation: AAX46416.1.
BC109605 mRNA. Translation: AAI09606.1.
RefSeqiNP_001020377.2. NM_001025206.2.
UniGeneiBt.64629.

Genome annotation databases

EnsembliENSBTAT00000044947; ENSBTAP00000042386; ENSBTAG00000017710.
GeneIDi281748.
KEGGibta:281748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ058610 mRNA. Translation: AAY83884.1.
DQ058603 Genomic DNA. Translation: AAY83878.1.
BT021569 mRNA. Translation: AAX46416.1.
BC109605 mRNA. Translation: AAI09606.1.
RefSeqiNP_001020377.2. NM_001025206.2.
UniGeneiBt.64629.

3D structure databases

ProteinModelPortaliQ58DM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042386.

Proteomic databases

PaxDbiQ58DM8.
PeptideAtlasiQ58DM8.
PRIDEiQ58DM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000044947; ENSBTAP00000042386; ENSBTAG00000017710.
GeneIDi281748.
KEGGibta:281748.

Organism-specific databases

CTDi1892.

Phylogenomic databases

eggNOGiKOG1680. Eukaryota.
COG1024. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG010157.
InParanoidiQ58DM8.
KOiK07511.
OMAiVSKIYPV.
OrthoDBiEOG091G0MVQ.
TreeFamiTF314497.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciMetaCyc:MONOMER-11697.
ReactomeiR-BTA-77310. Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA.
R-BTA-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
R-BTA-77348. Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
R-BTA-77350. Beta oxidation of hexanoyl-CoA to butanoyl-CoA.
R-BTA-77352. Beta oxidation of butanoyl-CoA to acetyl-CoA.
SABIO-RKQ58DM8.

Gene expression databases

BgeeiENSBTAG00000017710.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECHM_BOVIN
AccessioniPrimary (citable) accession number: Q58DM8
Secondary accession number(s): Q2TBV2, Q4PS76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: April 26, 2005
Last modified: October 5, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.