Q58DM8 (ECHM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-CoA hydratase, mitochondrial
EC=4.2.1.17

Alternative name(s):
Enoyl-CoA hydratase 1
Short-chain enoyl-CoA hydratase
Short name=SCEH

Gene names

Name:

ECHS1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Homohexamer; dimer of trimers By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Lyase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 290	263	Enoyl-CoA hydratase, mitochondrial		PRO_0000007410
Regions
	Region	98 – 101	4	Substrate binding By similarity
Sites
	Binding site	141	1	Substrate; via amide nitrogen By similarity
	Site	164	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	101	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	198	1	I → N in AAI09606. Ref.3
	Sequence conflict	269 – 277	9	TEDRKEGMA → PKTGRKAWP in AAY83884. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q58DM8 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 30CAD9F7314AD50F	FASTA	290	31,243
	10 20 30 40 50 60 MAALRALLPR VRAPLRPWLF CPVQRSFASS AAFEYIITAK KGRNSNVGLI QLNRPKALNA 70 80 90 100 110 120 LCNGLIVELN QALQAFEEDP AVGAIVLTGG EKVFAAGADI KEMQSLTFQN CYSGGFLSHW 130 140 150 160 170 180 DQLTRVKKPV IAAVNGYALG GGCELAMMCD IIYAGEKAQF GQPEILIGTI PGAGGTQRLT 190 200 210 220 230 240 RAVGKSLAME MVLTGDRISA QDAKQAGLVS KIFPVETVVE EAIQCAEKIA SNSKIVTAMA 250 260 270 280 290 KESVNAAFEM TLAEGVKLEK KLFYSTFATE DRKEGMAAFV EKRKANFKDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative mapping of the bovine SPRN locus." Ferretti L., Uboldi C., Del Vecchio I., Eggen A., Brunner R., Iannuzzi L. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ058610 mRNA. Translation: AAY83884.1. DQ058603 Genomic DNA. Translation: AAY83878.1. BT021569 mRNA. Translation: AAX46416.1. BC109605 mRNA. Translation: AAI09606.1.
IPI	IPI00701876.
RefSeq	NP_001020377.2. NM_001025206.2.
UniGene	Bt.64629.
3D structure databases
ProteinModelPortal	Q58DM8.
SMR	Q58DM8. Positions 31-290.
ModBase	Search...
Protein-protein interaction databases
STRING	Q58DM8.
Proteomic databases
PRIDE	Q58DM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000044947; ENSBTAP00000042386; ENSBTAG00000017710.
GeneID	281748.
KEGG	bta:281748.
Organism-specific databases
CTD	1892.
Phylogenomic databases
eggNOG	maNOG05271.
GeneTree	ENSGT00580000081296.
HOVERGEN	HBG010157.
InParanoid	Q58DM8.
OMA	TGNMINA.
OrthoDB	EOG4P2Q32.
PhylomeDB	Q58DM8.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11697.
Family and domain databases
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Gene3D	G3DSA:1.10.12.10. Crontonase_C. 1 hit.
KO	K07511.
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHM_BOVIN

Accession

Primary (citable) accession number: Q58DM8
Secondary accession number(s): Q2TBV2, Q4PS76

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	April 26, 2005
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program