Reviewed, UniProtKB/Swiss-Prot Q58DL1 (ARGI2_BOVIN)

Names and origin

Protein names

Recommended name:
    Arginase-2, mitochondrial
      Short name=Arginase II
    EC=3.5.3.1

Gene names

Name:

ARG2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis By similarity.
Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subunit structure	Homotrimer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism Urea cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arginase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 354	332	Arginase-2, mitochondrial		PRO_0000041752
Regions
	Region	145 – 149	5	Substrate binding By similarity
	Region	156 – 158	3	Substrate binding By similarity
Sites
	Metal binding	120	1	Manganese 1 By similarity
	Metal binding	143	1	Manganese 1 By similarity
	Metal binding	143	1	Manganese 2 By similarity
	Metal binding	145	1	Manganese 2 By similarity
	Metal binding	147	1	Manganese 1 By similarity
	Metal binding	251	1	Manganese 1 By similarity
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	253	1	Manganese 2 By similarity
	Binding site	202	1	Substrate By similarity
	Binding site	296	1	Substrate By similarity
Experimental info
	Sequence conflict	276	1	G → D in AAI33644. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q58DL1-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: E6DFBC08F32EEA04	FASTA	354	38,616
	10 20 30 40 50 60 MSLRSHLSRL LRTQVHSVRK KSVHSVAVIG APFSQGQKRK GVEYGPAAVR XAGLMKRLSD 70 80 90 100 110 120 LGCHLKDFGD LNFTPVPKDD LYNNLIVNPR SVGLANQELA EVVSRAVSGG YSCVTVGGDH 130 140 150 160 170 180 SLAIGTISGH ARHCPDLGVI WVDAHADINT PLTTSSGNLH GQPVSFLLRE LQDKVPQLPG 190 200 210 220 230 240 FSWIKPCISS PSIVYIGLRD VDPPEHFILK NYDIQYFSMR DIDRLGIQKV MEQTFDLLIG 250 260 270 280 290 300 KRQRPIHLSF DIDAFDPTLA PATGTPVVGG LTYREGIYIT EEIHSTGLLS ALDLVEVNPR 310 320 330 340 350 LAVSEEEAKA TASLAVDVIA SSFGQTREGG HIVYDQLPTP SSPDESESEE RVRI

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal cerebellum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT021586 mRNA. Translation: AAX46433.1. BC133643 mRNA. Translation: AAI33644.1.
IPI	IPI00700911.
RefSeq	NP_001017942.1.
UniGene	Bt.22399
3D structure databases
SMR	Q58DL1. Positions 24-329.
ModBase	Search...
Protein-protein interaction databases
STRING	Q58DL1.
Genome annotation databases
Ensembl	ENSBTAT00000002529; ENSBTAP00000002529; ENSBTAG00000001945; Bos taurus. [Genome view]
GeneID	518752.
KEGG	bta:518752.
Organism-specific databases
CTD	518752.
Phylogenomic databases
HOVERGEN	Q58DL1.
Enzyme and pathway databases
BRENDA	3.5.3.1. 251.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI2_BOVIN

Accession

Primary (citable) accession number: Q58DL1
Secondary accession number(s): A2VEA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	April 26, 2005
Last modified:	September 1, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)