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Q58DK5

- HEM2_BOVIN

UniProt

Q58DK5 - HEM2_BOVIN

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Protein

Delta-aminolevulinic acid dehydratase

Gene
ALAD
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 8 zinc ions per octamer. Requires four zinc ions per octamer for full catalytic activity. Can bind up to 2 zinc ions per subunit By similarity.

Enzyme regulationi

Can alternate between a fully active homooctamer and a low-activity homohexamer. A bound magnesium ion may promote the assembly of the fully active homooctamer. The magnesium-binding site is absent in the low-activity homohexamer. Inhibited by compounds that favor the hexameric state. Inhibited by divalent lead ions. The lead ions partially displace the zinc cofactor By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Zinc 1; catalytic By similarity
Metal bindingi124 – 1241Zinc 1; catalytic By similarity
Metal bindingi131 – 1311Zinc 2 By similarity
Metal bindingi132 – 1321Zinc 1; catalytic By similarity
Active sitei199 – 1991Schiff-base intermediate with substrate By similarity
Binding sitei209 – 2091Substrate 1 By similarity
Binding sitei221 – 2211Substrate 1 By similarity
Metal bindingi223 – 2231Zinc 2 By similarity
Active sitei252 – 2521Schiff-base intermediate with substrate By similarity
Binding sitei279 – 2791Substrate 2 By similarity
Binding sitei318 – 3181Substrate 2 By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. cellular response to interleukin-4 Source: Ensembl
  2. heme biosynthetic process Source: UniProtKB
  3. protein homooligomerization Source: Ensembl
  4. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_212036. Heme biosynthesis.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:ALAD
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 8

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Delta-aminolevulinic acid dehydratasePRO_0000328038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-succinyllysine By similarity
Modified residuei215 – 2151Phosphoserine By similarity
Modified residuei252 – 2521N6-succinyllysine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ58DK5.
PRIDEiQ58DK5.

Interactioni

Subunit structurei

Homooctamer; active form. Homohexamer; low activity form By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000315.

Structurei

3D structure databases

ProteinModelPortaliQ58DK5.
SMRiQ58DK5. Positions 1-328.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

eggNOGiCOG0113.
GeneTreeiENSGT00390000006998.
HOGENOMiHOG000020323.
HOVERGENiHBG001222.
InParanoidiQ58DK5.
KOiK01698.
OMAiDHPTACY.
OrthoDBiEOG751NFP.
TreeFamiTF300665.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58DK5-1 [UniParc]FASTAAdd to Basket

« Hide

MHPQSVLHSG YFHPLLRNWQ TAATSLSASN LIYPIFVTDV PDDKQPIASL    50
PGVARYGVNR LEEMLKPLVE EGLRCVLIFG VPSRVPKDER GSAADSEDSP 100
AIEAIRLLRK NFPSLLVACD VCLCPYTSHG HCGLLSENGS FQAEESRQRL 150
AEVALAYAKA GCQVVAPSDM MDGRVEAIKE ALMAHGFGNR VSVMSYSAKF 200
ASCFYGPFRD AAQSSPAFGD RRCYQLPPGA RGLALRAVDR DVREGADLLM 250
VKPGTPYLDI VREVKNKHPE LPLAVYHVSG EFAMLWHGAQ AGAFDLKAAV 300
LEVMTAFRRA GADVIITYYT PQLLQWLKE 329
Length:329
Mass (Da):36,081
Last modified:April 26, 2005 - v1
Checksum:i8BD0E141749B6465
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT021592 mRNA. Translation: AAX46439.1.
RefSeqiNP_001014895.1. NM_001014895.1.
UniGeneiBt.37574.

Genome annotation databases

EnsembliENSBTAT00000000315; ENSBTAP00000000315; ENSBTAG00000000251.
GeneIDi510679.
KEGGibta:510679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT021592 mRNA. Translation: AAX46439.1 .
RefSeqi NP_001014895.1. NM_001014895.1.
UniGenei Bt.37574.

3D structure databases

ProteinModelPortali Q58DK5.
SMRi Q58DK5. Positions 1-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000000315.

Proteomic databases

PaxDbi Q58DK5.
PRIDEi Q58DK5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000000315 ; ENSBTAP00000000315 ; ENSBTAG00000000251 .
GeneIDi 510679.
KEGGi bta:510679.

Organism-specific databases

CTDi 210.

Phylogenomic databases

eggNOGi COG0113.
GeneTreei ENSGT00390000006998.
HOGENOMi HOG000020323.
HOVERGENi HBG001222.
InParanoidi Q58DK5.
KOi K01698.
OMAi DHPTACY.
OrthoDBi EOG751NFP.
TreeFami TF300665.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
Reactomei REACT_212036. Heme biosynthesis.

Miscellaneous databases

NextBioi 20869564.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiHEM2_BOVIN
AccessioniPrimary (citable) accession number: Q58DK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 26, 2005
Last modified: September 3, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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