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Q58DK5

- HEM2_BOVIN

UniProt

Q58DK5 - HEM2_BOVIN

Protein

Delta-aminolevulinic acid dehydratase

Gene

ALAD

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 8 zinc ions per octamer. Requires four zinc ions per octamer for full catalytic activity. Can bind up to 2 zinc ions per subunit By similarity.By similarity

    Enzyme regulationi

    Can alternate between a fully active homooctamer and a low-activity homohexamer. A bound magnesium ion may promote the assembly of the fully active homooctamer. The magnesium-binding site is absent in the low-activity homohexamer. Inhibited by compounds that favor the hexameric state. Inhibited by divalent lead ions. The lead ions partially displace the zinc cofactor By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi122 – 1221Zinc 1; catalyticBy similarity
    Metal bindingi124 – 1241Zinc 1; catalyticBy similarity
    Metal bindingi131 – 1311Zinc 2By similarity
    Metal bindingi132 – 1321Zinc 1; catalyticBy similarity
    Active sitei199 – 1991Schiff-base intermediate with substrateBy similarity
    Binding sitei209 – 2091Substrate 1By similarity
    Binding sitei221 – 2211Substrate 1By similarity
    Metal bindingi223 – 2231Zinc 2By similarity
    Active sitei252 – 2521Schiff-base intermediate with substrateBy similarity
    Binding sitei279 – 2791Substrate 2By similarity
    Binding sitei318 – 3181Substrate 2By similarity

    GO - Molecular functioni

    1. lead ion binding Source: UniProtKB
    2. porphobilinogen synthase activity Source: UniProtKB
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to interleukin-4 Source: Ensembl
    2. heme biosynthetic process Source: UniProtKB
    3. protein homooligomerization Source: Ensembl
    4. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_212036. Heme biosynthesis.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:ALAD
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 8

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Delta-aminolevulinic acid dehydratasePRO_0000328038Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991N6-succinyllysineBy similarity
    Modified residuei215 – 2151PhosphoserineBy similarity
    Modified residuei252 – 2521N6-succinyllysineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ58DK5.
    PRIDEiQ58DK5.

    Interactioni

    Subunit structurei

    Homooctamer; active form. Homohexamer; low activity form By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000000315.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58DK5.
    SMRiQ58DK5. Positions 1-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    GeneTreeiENSGT00390000006998.
    HOGENOMiHOG000020323.
    HOVERGENiHBG001222.
    InParanoidiQ58DK5.
    KOiK01698.
    OMAiDHPTACY.
    OrthoDBiEOG751NFP.
    TreeFamiTF300665.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q58DK5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPQSVLHSG YFHPLLRNWQ TAATSLSASN LIYPIFVTDV PDDKQPIASL    50
    PGVARYGVNR LEEMLKPLVE EGLRCVLIFG VPSRVPKDER GSAADSEDSP 100
    AIEAIRLLRK NFPSLLVACD VCLCPYTSHG HCGLLSENGS FQAEESRQRL 150
    AEVALAYAKA GCQVVAPSDM MDGRVEAIKE ALMAHGFGNR VSVMSYSAKF 200
    ASCFYGPFRD AAQSSPAFGD RRCYQLPPGA RGLALRAVDR DVREGADLLM 250
    VKPGTPYLDI VREVKNKHPE LPLAVYHVSG EFAMLWHGAQ AGAFDLKAAV 300
    LEVMTAFRRA GADVIITYYT PQLLQWLKE 329
    Length:329
    Mass (Da):36,081
    Last modified:April 26, 2005 - v1
    Checksum:i8BD0E141749B6465
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT021592 mRNA. Translation: AAX46439.1.
    RefSeqiNP_001014895.1. NM_001014895.1.
    UniGeneiBt.37574.

    Genome annotation databases

    EnsembliENSBTAT00000000315; ENSBTAP00000000315; ENSBTAG00000000251.
    GeneIDi510679.
    KEGGibta:510679.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT021592 mRNA. Translation: AAX46439.1 .
    RefSeqi NP_001014895.1. NM_001014895.1.
    UniGenei Bt.37574.

    3D structure databases

    ProteinModelPortali Q58DK5.
    SMRi Q58DK5. Positions 1-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000000315.

    Proteomic databases

    PaxDbi Q58DK5.
    PRIDEi Q58DK5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000000315 ; ENSBTAP00000000315 ; ENSBTAG00000000251 .
    GeneIDi 510679.
    KEGGi bta:510679.

    Organism-specific databases

    CTDi 210.

    Phylogenomic databases

    eggNOGi COG0113.
    GeneTreei ENSGT00390000006998.
    HOGENOMi HOG000020323.
    HOVERGENi HBG001222.
    InParanoidi Q58DK5.
    KOi K01698.
    OMAi DHPTACY.
    OrthoDBi EOG751NFP.
    TreeFami TF300665.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    Reactomei REACT_212036. Heme biosynthesis.

    Miscellaneous databases

    NextBioi 20869564.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiHEM2_BOVIN
    AccessioniPrimary (citable) accession number: Q58DK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3