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Q58DK4 (DHAK_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

Including the following 2 domains:

  1. ATP-dependent dihydroxyacetone kinase
    Short name=DHA kinase
    EC=2.7.1.28
    EC=2.7.1.29
    Alternative name(s):
    Glycerone kinase
    Triokinase
    Triose kinase
  2. FAD-AMP lyase (cyclizing)
    EC=4.6.1.15
    Alternative name(s):
    FAD-AMP lyase (cyclic FMN forming)
    FMN cyclase
Gene names
Name:DAK
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate By similarity.

Catalytic activity

ATP + glycerone = ADP + glycerone phosphate.

ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

FAD = AMP + riboflavin cyclic-4',5'-phosphate.

Cofactor

Magnesium By similarity.

Manganese or cobalt; for FAD-AMP lyase activity By similarity.

Enzyme regulation

Each activity is inhibited by the substrate(s) of the other By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Contains 1 DhaK domain.

Contains 1 DhaL domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
PRO_0000121524

Regions

Domain9 – 336328DhaK
Domain372 – 571200DhaL
Nucleotide binding401 – 4044ATP By similarity
Nucleotide binding446 – 4472ATP By similarity
Nucleotide binding494 – 4952ATP By similarity
Nucleotide binding556 – 5583ATP By similarity
Region56 – 594Dihydroxyacetone binding By similarity

Sites

Active site2211Tele-hemiaminal-histidine intermediate By similarity
Binding site1091Dihydroxyacetone By similarity
Binding site1141Dihydroxyacetone By similarity
Binding site4861ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58DK4 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 04F870A1C05001E4

FASTA57859,124
        10         20         30         40         50         60 
MTSKKLVNSV AGCADDALAG LVACNPSLQL LQGHRVALRS DLDSLKGRVA LLSGGGSGHE 

        70         80         90        100        110        120 
PAHAGFIGKG MLTGVIAGAV FTSPAVGSIL AAIRAVAQAG TVGTLLIVKN YTGDRLNFGL 

       130        140        150        160        170        180 
AREQARAEGI PVEMVVVGDD SAFTVLKKAG RRGLCGTVLI HKVAGALAEA GVGLEEITDR 

       190        200        210        220        230        240 
VSVVAKAMGT LGVSLSSCSV PGSKPTFELS ADEVELGLGI HGEAGVRRIK MATANEIVAL 

       250        260        270        280        290        300 
MLDHMTSSSN ASHVPVPPGS SVVLMVNNLG GLSFLELGII ADAAVCSLEG HGVKIARALV 

       310        320        330        340        350        360 
GTFMSALEMP GVSLTLLLVD EPLLKLIDAE TTASAWPNVA KVWVTGRKRS RAAPTEPLAA 

       370        380        390        400        410        420 
PDSTTAAGEA SKQMVLVLEW VCTTLLGLEE HLNALDRAAG DGDCGTTHSR AARAIXGWLK 

       430        440        450        460        470        480 
EGPPPASPAQ LLSKLSFLLL EKMGGSSGAL YGLFLTAAAQ PLKAKTDLPA WSAAMDAGLE 

       490        500        510        520        530        540 
AMQKYGKAAP GDRTMLDSLW AAGQELQAWK SPGANMLQIL TKAVKSAEAA AEATKNMEAG 

       550        560        570 
AGRASYISSA RLDQPDPGAV AAAAILRAIL EVLQSQGA 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT021593 mRNA. Translation: AAX46440.1.
UniGeneBt.3266.

3D structure databases

ProteinModelPortalQ58DK4.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ58DK4.
PRIDEQ58DK4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG2376.
HOGENOMHOG000234158.
HOVERGENHBG079502.
InParanoidQ58DK4.

Family and domain databases

InterProIPR004006. Dak1.
IPR012734. DhaK_ATP.
IPR004007. DhaL_dom.
[Graphical view]
PfamPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SUPFAMSSF101473. SSF101473. 1 hit.
TIGRFAMsTIGR02361. dak_ATP. 1 hit.
PROSITEPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHAK_BOVIN
AccessionPrimary (citable) accession number: Q58DK4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: April 26, 2005
Last modified: January 22, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families