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Q58DH9

- NAGAB_BOVIN

UniProt

Q58DH9 - NAGAB_BOVIN

Protein

Alpha-N-acetylgalactosaminidase

Gene

NAGA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids By similarity.By similarity

    Catalytic activityi

    Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei154 – 1541SubstrateBy similarity
    Active sitei156 – 1561NucleophileBy similarity
    Binding sitei188 – 1881SubstrateBy similarity
    Binding sitei213 – 2131SubstrateBy similarity
    Active sitei217 – 2171Proton donorBy similarity
    Binding sitei217 – 2171SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-N-acetylgalactosaminidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate catabolic process Source: UniProtKB
    2. glycolipid catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH27. Glycoside Hydrolase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
    Alternative name(s):
    Alpha-galactosidase B
    Gene namesi
    Name:NAGA
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Lysosome By similarity

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717By similarityAdd
    BLAST
    Chaini18 – 411394Alpha-N-acetylgalactosaminidasePRO_0000001017Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi38 ↔ 80By similarity
    Disulfide bondi42 ↔ 49By similarity
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi127 ↔ 158By similarity
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi187 ↔ 209By similarity
    Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
    Modified residuei322 – 3221PhosphoserineBy similarity
    Glycosylationi359 – 3591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ58DH9.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ58DH9.
    SMRiQ58DH9. Positions 18-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni78 – 792Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68897.
    HOGENOMiHOG000161224.
    HOVERGENiHBG001989.
    InParanoidiQ58DH9.
    KOiK01204.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PRINTSiPR00740. GLHYDRLASE27.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58DH9-1 [UniParc]FASTAAdd to Basket

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    MLLKTVLLLA LASQVLVLEN GLLRKPPMGW LAWERFRCNI DCSEDPKNCI    50
    SEQLFMEMAD RLAQDGWRDL GYVYLNIDDC WIGGRDAKGN LVPDRKRFPH 100
    GIAFLADYAH SLGLKLGIYE DLGNFTCMGY PGTTLDKVVQ DAQTFAEWKV 150
    DMLKLDGCYS TPQERAEGYP KMAAALNATG RPIAFSCSWP AYEGGLPPKV 200
    NYTLLADICN LWRNFDDIQD SWRSVLSVLD WFVTHQDVLQ PIAGPGHWND 250
    PDMLLIGNFG LSFEQAQAQM ALWTVLAAPL FMSTDLRTIS AQNMDILQNP 300
    LMIKINQDPL GIQGRRILKE KSHIEVYLRP LASEASAIVF FSRRMDMPYH 350
    YHSSLARLNF SSSVVYEAQD VYTGDIISGL QDKTNFTVII NPSGVVMWYL 400
    YPIRKLEIPQ Q 411
    Length:411
    Mass (Da):46,533
    Last modified:April 26, 2005 - v1
    Checksum:i69039FB69B869144
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT021618 mRNA. Translation: AAX46465.1.
    RefSeqiNP_001039814.1. NM_001046349.1.
    UniGeneiBt.57867.

    Genome annotation databases

    GeneIDi533357.
    KEGGibta:533357.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT021618 mRNA. Translation: AAX46465.1 .
    RefSeqi NP_001039814.1. NM_001046349.1.
    UniGenei Bt.57867.

    3D structure databases

    ProteinModelPortali Q58DH9.
    SMRi Q58DH9. Positions 18-402.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL5079.

    Protein family/group databases

    CAZyi GH27. Glycoside Hydrolase Family 27.

    Proteomic databases

    PRIDEi Q58DH9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 533357.
    KEGGi bta:533357.

    Organism-specific databases

    CTDi 4668.

    Phylogenomic databases

    eggNOGi NOG68897.
    HOGENOMi HOG000161224.
    HOVERGENi HBG001989.
    InParanoidi Q58DH9.
    KOi K01204.

    Miscellaneous databases

    NextBioi 20876014.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PRINTSi PR00740. GLHYDRLASE27.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiNAGAB_BOVIN
    AccessioniPrimary (citable) accession number: Q58DH9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3