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Q58DH9 (NAGAB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-N-acetylgalactosaminidase

EC=3.2.1.49
Alternative name(s):
Alpha-galactosidase B
Gene names
Name:NAGA
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids By similarity.

Catalytic activity

Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.

Subunit structure

Homodimer By similarity.

Subcellular location

Lysosome By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 By similarity
Chain18 – 411394Alpha-N-acetylgalactosaminidase
PRO_0000001017

Regions

Region78 – 792Substrate binding By similarity

Sites

Active site1561Nucleophile By similarity
Active site2171Proton donor By similarity
Binding site1541Substrate By similarity
Binding site1881Substrate By similarity
Binding site2131Substrate By similarity
Binding site2171Substrate By similarity

Amino acid modifications

Modified residue3221Phosphoserine By similarity
Glycosylation1241N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation2011N-linked (GlcNAc...) Potential
Glycosylation3591N-linked (GlcNAc...) Potential
Glycosylation3851N-linked (GlcNAc...) Potential
Disulfide bond38 ↔ 80 By similarity
Disulfide bond42 ↔ 49 By similarity
Disulfide bond127 ↔ 158 By similarity
Disulfide bond187 ↔ 209 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58DH9 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 69039FB69B869144

FASTA41146,533
        10         20         30         40         50         60 
MLLKTVLLLA LASQVLVLEN GLLRKPPMGW LAWERFRCNI DCSEDPKNCI SEQLFMEMAD 

        70         80         90        100        110        120 
RLAQDGWRDL GYVYLNIDDC WIGGRDAKGN LVPDRKRFPH GIAFLADYAH SLGLKLGIYE 

       130        140        150        160        170        180 
DLGNFTCMGY PGTTLDKVVQ DAQTFAEWKV DMLKLDGCYS TPQERAEGYP KMAAALNATG 

       190        200        210        220        230        240 
RPIAFSCSWP AYEGGLPPKV NYTLLADICN LWRNFDDIQD SWRSVLSVLD WFVTHQDVLQ 

       250        260        270        280        290        300 
PIAGPGHWND PDMLLIGNFG LSFEQAQAQM ALWTVLAAPL FMSTDLRTIS AQNMDILQNP 

       310        320        330        340        350        360 
LMIKINQDPL GIQGRRILKE KSHIEVYLRP LASEASAIVF FSRRMDMPYH YHSSLARLNF 

       370        380        390        400        410 
SSSVVYEAQD VYTGDIISGL QDKTNFTVII NPSGVVMWYL YPIRKLEIPQ Q 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT021618 mRNA. Translation: AAX46465.1.
RefSeqNP_001039814.1. NM_001046349.1.
UniGeneBt.57867.

3D structure databases

ProteinModelPortalQ58DH9.
SMRQ58DH9. Positions 18-402.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL5079.

Protein family/group databases

CAZyGH27. Glycoside Hydrolase Family 27.

Proteomic databases

PRIDEQ58DH9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID533357.
KEGGbta:533357.

Organism-specific databases

CTD4668.

Phylogenomic databases

eggNOGNOG68897.
HOGENOMHOG000161224.
HOVERGENHBG001989.
InParanoidQ58DH9.
KOK01204.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSPR00740. GLHYDRLASE27.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20876014.

Entry information

Entry nameNAGAB_BOVIN
AccessionPrimary (citable) accession number: Q58DH9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries