Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eyes absent homolog 2

Gene

EYA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731NucleophileBy similarity
Metal bindingi273 – 2731MagnesiumBy similarity
Active sitei275 – 2751Proton donorBy similarity
Metal bindingi275 – 2751Magnesium; via carbonyl oxygenBy similarity
Metal bindingi501 – 5011MagnesiumBy similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB
  2. protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  3. histone dephosphorylation Source: UniProtKB
  4. mitochondrial outer membrane permeabilization Source: Ensembl
  5. multicellular organismal development Source: UniProtKB-KW
  6. peptidyl-tyrosine dephosphorylation Source: GOC
  7. regulation of transcription, DNA-templated Source: UniProtKB-KW
  8. striated muscle tissue development Source: Ensembl
  9. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Developmental protein, Hydrolase, Protein phosphatase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eyes absent homolog 2 (EC:3.1.3.48)
Gene namesi
Name:EYA2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Retained in the cytoplasm via interaction with GNAZ and GNAI2. Interaction with SIX1, SIX2, SIX4 or SIX5 is required for translocation to the nucleus (By similarity).By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. mitochondrion Source: GOC
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Eyes absent homolog 2PRO_0000244420Add
BLAST

Proteomic databases

PRIDEiQ58DB6.

Interactioni

Subunit structurei

Interacts with DACH2 and SIX1, and probably with SIX2, SIX4 and SIX5. Interacts with CAPN8. Interacts with GNAZ and GNAI2; this precludes interaction with SIX1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017737.

Structurei

3D structure databases

ProteinModelPortaliQ58DB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG297494.
GeneTreeiENSGT00390000008860.
HOGENOMiHOG000293149.
HOVERGENiHBG002447.
InParanoidiQ58DB6.
KOiK17620.
OMAiIHSRPNC.
OrthoDBiEOG7DNNTZ.
TreeFamiTF319337.

Family and domain databases

InterProiIPR028473. EYA2.
IPR006545. EYA_dom.
IPR028472. EYA_fam.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR10190. PTHR10190. 1 hit.
PTHR10190:SF7. PTHR10190:SF7. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01658. EYA-cons_domain. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58DB6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLELLVSASL TVNSDRPGKL KPSRADADVW TLSDREGITT SARSVSQLFA
60 70 80 90 100
RPCPRVPPGQ PPSAMAAYSQ TQYSAGIQQA TPYTAYPPPA QAYGIPSYSI
110 120 130 140 150
KTEDSLNHSP GQSGFLSYGS SFSTPASGQS PYTYQMHGTA GIYQGANGLT
160 170 180 190 200
NAAGFGTVHQ DYPSYPGFPQ SQYSQYYSSS YNPPYVPASS ICPSPLSTST
210 220 230 240 250
YVLQEASHNI PSQSSESLGG EYNTHNGPST PAKEGDTDRP PRASDGKLRG
260 270 280 290 300
RSKRSSDPSP AGDNEIERVF VWDLDETIII FHSLLTGTFA SRYGKDTTAS
310 320 330 340 350
VRIGLMMEEM IFNLADTHLF FNDLEDCDQI HVDDVSSDDN GQDLSTYNFS
360 370 380 390 400
ADGFHSSAPG ANLCLGSGVH GGVDWMRKLA FRYRRVKEMY NTYKNNVGGL
410 420 430 440 450
IGAPKRETWL QLRAELEALT DLWLTHSLKA LNLINSRPNC VNVLVTTTQL
460 470 480 490 500
IPALAKVLLY GLGSVFPIEN IYSATKTGKE SCFERIMQRF GRKAVYIVIG
510 520 530
DGVEEEQGAK KHNMPFWRIS CHADLEALRH ALELEYL
Length:537
Mass (Da):58,807
Last modified:April 26, 2005 - v1
Checksum:i081635FDF8BEE2DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021681 mRNA. Translation: AAX46528.1.
RefSeqiNP_001030541.1. NM_001035464.1.
UniGeneiBt.26339.

Genome annotation databases

EnsembliENSBTAT00000017737; ENSBTAP00000017737; ENSBTAG00000013336.
GeneIDi615264.
KEGGibta:615264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021681 mRNA. Translation: AAX46528.1.
RefSeqiNP_001030541.1. NM_001035464.1.
UniGeneiBt.26339.

3D structure databases

ProteinModelPortaliQ58DB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017737.

Proteomic databases

PRIDEiQ58DB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017737; ENSBTAP00000017737; ENSBTAG00000013336.
GeneIDi615264.
KEGGibta:615264.

Organism-specific databases

CTDi2139.

Phylogenomic databases

eggNOGiNOG297494.
GeneTreeiENSGT00390000008860.
HOGENOMiHOG000293149.
HOVERGENiHBG002447.
InParanoidiQ58DB6.
KOiK17620.
OMAiIHSRPNC.
OrthoDBiEOG7DNNTZ.
TreeFamiTF319337.

Miscellaneous databases

NextBioi20899533.

Family and domain databases

InterProiIPR028473. EYA2.
IPR006545. EYA_dom.
IPR028472. EYA_fam.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR10190. PTHR10190. 1 hit.
PTHR10190:SF7. PTHR10190:SF7. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01658. EYA-cons_domain. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiEYA2_BOVIN
AccessioniPrimary (citable) accession number: Q58DB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: April 26, 2005
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.