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Q58DB6 (EYA2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eyes absent homolog 2

EC=3.1.3.48
Gene names
Name:EYA2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length537 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Cofactor

Binds 1 Mg2+ ion per subunit By similarity.

Subunit structure

Interacts with DACH2 and SIX1, and probably with SIX2, SIX4 and SIX5. Interacts with CAPN8. Interacts with GNAZ and GNAI2; this precludes interaction with SIX1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Retained in the cytoplasm via interaction with GNAZ and GNAI2. Interaction with SIX1, SIX2, SIX4 or SIX5 is required for translocation to the nucleus By similarity.

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. EYA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 537537Eyes absent homolog 2
PRO_0000244420

Sites

Active site2731Nucleophile By similarity
Active site2751Proton donor By similarity
Metal binding2731Magnesium By similarity
Metal binding2751Magnesium; via carbonyl oxygen By similarity
Metal binding5011Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58DB6 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 081635FDF8BEE2DF

FASTA53758,807
        10         20         30         40         50         60 
MLELLVSASL TVNSDRPGKL KPSRADADVW TLSDREGITT SARSVSQLFA RPCPRVPPGQ 

        70         80         90        100        110        120 
PPSAMAAYSQ TQYSAGIQQA TPYTAYPPPA QAYGIPSYSI KTEDSLNHSP GQSGFLSYGS 

       130        140        150        160        170        180 
SFSTPASGQS PYTYQMHGTA GIYQGANGLT NAAGFGTVHQ DYPSYPGFPQ SQYSQYYSSS 

       190        200        210        220        230        240 
YNPPYVPASS ICPSPLSTST YVLQEASHNI PSQSSESLGG EYNTHNGPST PAKEGDTDRP 

       250        260        270        280        290        300 
PRASDGKLRG RSKRSSDPSP AGDNEIERVF VWDLDETIII FHSLLTGTFA SRYGKDTTAS 

       310        320        330        340        350        360 
VRIGLMMEEM IFNLADTHLF FNDLEDCDQI HVDDVSSDDN GQDLSTYNFS ADGFHSSAPG 

       370        380        390        400        410        420 
ANLCLGSGVH GGVDWMRKLA FRYRRVKEMY NTYKNNVGGL IGAPKRETWL QLRAELEALT 

       430        440        450        460        470        480 
DLWLTHSLKA LNLINSRPNC VNVLVTTTQL IPALAKVLLY GLGSVFPIEN IYSATKTGKE 

       490        500        510        520        530 
SCFERIMQRF GRKAVYIVIG DGVEEEQGAK KHNMPFWRIS CHADLEALRH ALELEYL 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT021681 mRNA. Translation: AAX46528.1.
RefSeqNP_001030541.1. NM_001035464.1.
UniGeneBt.26339.

3D structure databases

ProteinModelPortalQ58DB6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000017737.

Proteomic databases

PRIDEQ58DB6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000017737; ENSBTAP00000017737; ENSBTAG00000013336.
GeneID615264.
KEGGbta:615264.

Organism-specific databases

CTD2139.

Phylogenomic databases

eggNOGNOG297494.
GeneTreeENSGT00390000008860.
HOGENOMHOG000293149.
HOVERGENHBG002447.
InParanoidQ58DB6.
KOK17620.
OMAIHSRPNC.
OrthoDBEOG7DNNTZ.
TreeFamTF319337.

Family and domain databases

InterProIPR028473. EYA2.
IPR006545. EYA_dom.
IPR028472. EYA_fam.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERPTHR10190. PTHR10190. 1 hit.
PTHR10190:SF7. PTHR10190:SF7. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01658. EYA-cons_domain. 1 hit.
ProtoNetSearch...

Other

NextBio20899533.

Entry information

Entry nameEYA2_BOVIN
AccessionPrimary (citable) accession number: Q58DB6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: April 26, 2005
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families