Q58D55 (BGAL_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
EC=3.2.1.23

Alternative name(s):
Acid beta-galactosidase
Short name=Lactase

Gene names

Name:

GLB1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 27	5	By similarity		PRO_0000283035
	Chain	28 – 653	626	Beta-galactosidase		PRO_0000283036
Sites
	Active site	187	1	Proton donor Potential
	Active site	267	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	246	1	N-linked (GlcNAc...) Potential
	Glycosylation	463	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Glycosylation	554	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q58D55 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 37ED2284A4EB4F97	FASTA	653	73,413
	10 20 30 40 50 60 MPGVVRLLAL LLVPLLLGSA RGLHNATQRT FQIDYRRNRF LKDGQPFRYI SGSIHYFRVP 70 80 90 100 110 120 RFYWKDRLLK MKMAGLNAIQ TYVAWNFHEL QPGRYNFSGD HDVEHFIQLA HELGLLVILR 130 140 150 160 170 180 PGPYICAEWD MGGLPAWLLE KKSIVLRSSD PDYLAAVDKW LGVLLPKMRP LLYKNGGPII 190 200 210 220 230 240 TVQVENEYGS YLSCDYDYLR FLQKRFHDHL GEDVLLFTTD GVNERLLQCG ALQGLYATVD 250 260 270 280 290 300 FSPGTNLTAA FMLQRKFEPT GPLVNSEFYT GWLDHWGQRH STVSSKAVAF TLHDMLALGA 310 320 330 340 350 360 NVNMYMFIGG TNFAYWNGAN IPYQPQPTSY DYDAPLSEAG DLTEKYFALR DIIQKFAKVP 370 380 390 400 410 420 EGPIPPSTPK FAYGKVALNK LKTVEDALNI LCPSGPIKSV YPLTFIDVKQ YFGFVLYRTM 430 440 450 460 470 480 LPEDCSDPTP LSSPLSGVHD RAYVSVNGVA QGILERESVI TLNITGKAGA TLDLLVENMG 490 500 510 520 530 540 RVNYGSSIND FKGLVSNLTL GSKILTNWEI FPLDMEDAVR SHLGTWGGRD RGYHNKARAH 550 560 570 580 590 600 SPPTYALPTF YVGNFTIPSG IADLPQDTFI QFPGWTKGQV WINGFNLGRY WPVRGPQMTL 610 620 630 640 650 FVPQHILVTS TPNTIVVLEL EHAPCQDGGP ELCTVEFVDK PVFRTVQTHR HAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of lysosomal beta-galactosidase from bovine liver." Satoh Y., Yajima A., Uda Y. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB325580 mRNA. Translation: BAF64285.1. BT021742 mRNA. Translation: AAX46589.1.
IPI	IPI00699237.
RefSeq	NP_001030215.1. NM_001035043.1.
UniGene	Bt.6127.
3D structure databases
ProteinModelPortal	Q58D55.
ModBase	Search...
Protein-protein interaction databases
STRING	Q58D55.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q58D55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	507188.
KEGG	bta:507188.
Organism-specific databases
CTD	2720.
Phylogenomic databases
eggNOG	maNOG10463.
GeneTree	ENSGT00390000006586.
HOVERGEN	HBG004841.
InParanoid	Q58D55.
OrthoDB	EOG46MBJ7.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K12309.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_BOVIN

Accession

Primary (citable) accession number: Q58D55
Secondary accession number(s): A5LIP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 26, 2005
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program