Reviewed, UniProtKB/Swiss-Prot Q58CP0 (IDH3G_BOVIN)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

IDH3G

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Enzyme regulation	Activated by increasing ADP/ATP ratios and by Ca2+ By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Manganese Metal-binding NAD Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	isocitrate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: AgBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 392	353	Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial		PRO_0000236185
Regions
	Nucleotide binding	55 – 83	29	NAD Potential
	Nucleotide binding	308 – 315	8	ATP Potential
Sites
	Metal binding	253	1	Magnesium or manganese By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
	Site	173	1	Critical for catalysis By similarity
	Site	220	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	362	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58CP0-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 44F749F782E18EFD	FASTA	392	42,863
	10 20 30 40 50 60 MALKVATAAG GAVKAALRPA LLWRPWEVLG SHEAPRRSFS QQTIPPSAKY GGRHTVTMIP 70 80 90 100 110 120 GDGIGPELML HVKSVFRHAC VPVDFEEVHV SSTADEEDIR NAIMAIRRNR VALKGNIETN 130 140 150 160 170 180 HNLPPSHKSR NNILRTSLDL YANVIHCKSL PGVVTRHRDI DILIVRENTE GEYSSLEHES 190 200 210 220 230 240 VAGVVESLKI ITKAKSLRIA EYAFQLAQES GRKKVTAVHK ANIMKLGDGL FLQCCREVAA 250 260 270 280 290 300 RYPQITFENM IVDNTTMQLV SRPQQFDVMV MPNLYGNIVN NVCAGLVGGP GLVAGANYGH 310 320 330 340 350 360 VYAVFETATR NTGKSIANKN IANPTATLLA SCMMLDHLKL HSYATSIRKA VLASMDNENM 370 380 390 HTPDIGGQGT TSEAIQDIIR HIRVINGRAV EA

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT021907 mRNA. Translation: AAX46754.1. BC118275 mRNA. Translation: AAI18276.1.
IPI	IPI00709977.
RefSeq	NP_001069781.1.
UniGene	Bt.23357
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000001059. Bos taurus. [Contig view]
GeneID	614145.
KEGG	bta:614145.
Phylogenomic databases
HOVERGEN	Q58CP0.
Enzyme and pathway databases
BRENDA	1.1.1.41. 251.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH3G_BOVIN

Accession

Primary (citable) accession number: Q58CP0
Secondary accession number(s): Q148J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	April 26, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)