Q58A65 (JIP4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-Jun-amino-terminal kinase-interacting protein 4
Short name=JIP-4
Short name=JNK-interacting protein 4

Alternative name(s):
JNK-associated leucine-zipper protein
Short name=JLP
JNK/SAPK-associated protein 2
Short name=JSAP2
Mitogen-activated protein kinase 8-interacting protein 4
Sperm-associated antigen 9

Gene names

Name:	Spag9
Synonyms:	Jip4, Jsap2, Kiaa0516, Mapk8ip4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Ref.1 Ref.2
Subunit structure	Homooligomer. Interacts with MAX, MAPK8, MAPK14, MAPK10, MAPK14, MAP3K3, MYC, KNS2, and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Ref.1 Ref.2 Ref.8
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. Ref.1 Ref.2
Tissue specificity	Isoform 6 is highly expressed in brain, kidney, liver, heart. Ref.2
Induction	Up-regulated during neuronal differentiation. Ref.8
Post-translational modification	Phosphorylated by MAPK8 and MAPK14. Ref.2
Sequence similarities	Belongs to the JIP scaffold family.
Sequence caution	The sequence BAD32239.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI35376.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of JUN kinase activity Inferred from direct assay Ref.1. Source: MGI activation of MAPK activity Inferred from direct assay Ref.2. Source: MGI negative regulation of protein homodimerization activity Inferred from direct assay Ref.1. Source: MGI positive regulation of cell migration Inferred from electronic annotation. Source: Compara positive regulation of neuron differentiation Inferred from mutant phenotype PubMed 19244314. Source: MGI protein homooligomerization Inferred from direct assay Ref.2. Source: MGI retrograde transport, endosome to Golgi Inferred from electronic annotation. Source: Compara striated muscle cell differentiation Inferred from genetic interaction PubMed 20160094. Source: MGI
Cellular_component	cytosol Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from direct assay PubMed 19056739. Source: MGI
Molecular_function	JUN kinase binding Inferred from direct assay Ref.1Ref.2. Source: MGI kinesin binding Inferred from direct assay Ref.2. Source: MGI mitogen-activated protein kinase p38 binding Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LRRK2	Q5S007	2	EBI-6530207,EBI-5323863	From a different organism.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q58A65-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q58A65-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.

Isoform 3 (identifier: Q58A65-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.
     144-196: RLEEREAELK...QLEATAHSRI → MNPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     405-405: G → GEYSG

Note: No experimental confirmation available.

Isoform 4 (identifier: Q58A65-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.
     144-196: RLEEREAELK...QLEATAHSRI → MNPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     1175-1175: T → TVILHQGRLLGLRA

Note: No experimental confirmation available.

Isoform 5 (identifier: Q58A65-5)

The sequence of this isoform differs from the canonical sequence as follows:
     144-196: RLEEREAELK...QLEATAHSRI → MNPGCMLLFV...ALTQNLPRIL
     248-261: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: Q58A65-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-165: Missing.
     248-261: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1321	1321	C-Jun-amino-terminal kinase-interacting protein 4		PRO_0000234077
Regions
	Coiled coil	66 – 166	101	Potential
	Coiled coil	408 – 534	127	Potential
	Coiled coil	724 – 758	35	Potential
Amino acid modifications
	Modified residue	109	1	Phosphoserine By similarity
	Modified residue	183	1	Phosphoserine By similarity
	Modified residue	185	1	Phosphoserine By similarity
	Modified residue	194	1	Phosphoserine By similarity
	Modified residue	203	1	Phosphoserine Ref.11
	Modified residue	217	1	Phosphothreonine Ref.12
	Modified residue	244	1	Phosphothreonine Ref.10
	Modified residue	251	1	Phosphoserine By similarity
	Modified residue	265	1	Phosphoserine By similarity
	Modified residue	268	1	Phosphoserine By similarity
	Modified residue	272	1	Phosphoserine By similarity
	Modified residue	292	1	Phosphothreonine Ref.10
	Modified residue	311	1	Phosphoserine By similarity
	Modified residue	329	1	Phosphoserine By similarity
	Modified residue	332	1	Phosphoserine By similarity
	Modified residue	347	1	Phosphoserine By similarity
	Modified residue	348	1	Phosphothreonine By similarity
	Modified residue	365	1	Phosphothreonine Ref.12
	Modified residue	418	1	Phosphothreonine By similarity
	Modified residue	586	1	Phosphothreonine By similarity
	Modified residue	730	1	Phosphoserine Ref.9 Ref.11
	Modified residue	733	1	Phosphoserine Ref.9 Ref.10
Natural variations
	Alternative sequence	1 – 165	165	Missing in isoform 6.		VSP_018225
	Alternative sequence	1 – 143	143	Missing in isoform 3 and isoform 4.		VSP_018226
	Alternative sequence	144 – 196	53	RLEER…AHSRI → MNPGCMLLFVFGFVGGAVVI NSAILVSLSVLLLVHFSIST GVPALTQNLPRIL in isoform 3, isoform 4 and isoform 5.		VSP_018227
	Alternative sequence	248 – 261	14	Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6.		VSP_018228
	Alternative sequence	405	1	G → GEYSG in isoform 3.		VSP_018229
	Alternative sequence	1175	1	T → TVILHQGRLLGLRA in isoform 4.		VSP_018230
Experimental info
	Sequence conflict	241	1	R → C in AAN61564. Ref.1
	Sequence conflict	453	1	E → A in AAN61564. Ref.1
	Sequence conflict	468	1	E → G in BAE27980. Ref.5
	Sequence conflict	705	1	S → T in BAD93176. Ref.3
	Sequence conflict	1114	1	L → P in BAE27907. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: 93A6E97A62E0E92C	FASTA	1,321	146,219
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLEA TAHSRIRKER PISLGIFPLP AGDGLLTPDT QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKVSH SPEPPKAVEQ EDELSDISQG GSKATTPAST ANSDVSAIPP DTPSKEDNEG 310 320 330 340 350 360 FVKGTDTSNK SEISKHIEVQ VAQETRNVST ESGENEEKSE VQAIIESTPE LDMDKDLSGY 370 380 390 400 410 420 KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG ADLLGMGREV ENLILENTQL 430 440 450 460 470 480 LETKNALNVV KNDLIAKVDE LTCEKDVLQG ELEAVKQAKL KLEDKNRELE EELRKARAEA 490 500 510 520 530 540 EDARQKAKDD DDSDIPTAQR KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE 550 560 570 580 590 600 NPAMQEKKRS SIWQFFSRLF SSSSNATKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 610 620 630 640 650 660 GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVANG 670 680 690 700 710 720 QGETKMKNLP VPVYLRPLDE KDASMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDIAGLD 730 740 750 760 770 780 TEGSKQRSAS QSSLDKLDQE LKEQQKEFKN QEELSSQVWI CTSTHSTTKV IIIDAVQPGN 790 800 810 820 830 840 ILDSFTVCNS HVLCIASVPG ARETDYPAGE ELSESGQVDK ASLCGSMTSN SSAEMDSLLG 850 860 870 880 890 900 GITVVGCSTE GLTGAATSPS TNGASPVIEK PPEMETENSE VDENIPTAEE ATEATEGNAG 910 920 930 940 950 960 STEDTVDISQ PGVYTEHVFT DPLGVQIPED LSPVFQSSND SDVYKDQISV LPNEQDLARE 970 980 990 1000 1010 1020 EAQKMSSLLP TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK DSILSIVHVK GIVLVALADG 1030 1040 1050 1060 1070 1080 TLAIFHRGVD GQWDLSNYHL LDLGRPHHSI RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK 1090 1100 1110 1120 1130 1140 SFDAHPRKES QVRQLAWVGD GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK 1150 1160 1170 1180 1190 1200 LGFSFVRITA LMVSCNRLWV GTGNGVIISI PLTETNKTSG TPGNRPGSVI RVYGDENSDK 1210 1220 1230 1240 1250 1260 VTPGTFIPYC SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSGGADLTA DKAGSSAQEP 1270 1280 1290 1300 1310 1320 SSQTPLKSML VISGGEGYID FRMGDEGGES ELLGEDLPLE PSVTKAERSH LIVWQVMCGN E
	Isoform 2 [UniParc]. Checksum: 8C99195A5AFFC7BF	FASTA	1,307	144,704
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLEA TAHSRIRKER PISLGIFPLP AGDGLLTPDT QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDISQGGSKA TTPASTANSD VSAIPPDTPS KEDNEGFVKG TDTSNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTESGE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNVVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLED KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FFSRLFSSSS NATKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 610 620 630 640 650 660 EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVANGQGET KMKNLPVPVY 670 680 690 700 710 720 LRPLDEKDAS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DIAGLDTEGS KQRSASQSSL 730 740 750 760 770 780 DKLDQELKEQ QKEFKNQEEL SSQVWICTST HSTTKVIIID AVQPGNILDS FTVCNSHVLC 790 800 810 820 830 840 IASVPGARET DYPAGEELSE SGQVDKASLC GSMTSNSSAE MDSLLGGITV VGCSTEGLTG 850 860 870 880 890 900 AATSPSTNGA SPVIEKPPEM ETENSEVDEN IPTAEEATEA TEGNAGSTED TVDISQPGVY 910 920 930 940 950 960 TEHVFTDPLG VQIPEDLSPV FQSSNDSDVY KDQISVLPNE QDLAREEAQK MSSLLPTMWL 970 980 990 1000 1010 1020 GAQNGCLYVH SSVAQWRKCL HSIKLKDSIL SIVHVKGIVL VALADGTLAI FHRGVDGQWD 1030 1040 1050 1060 1070 1080 LSNYHLLDLG RPHHSIRCMT VVHDKVWCGY RNKIYVVQPK AMKIEKSFDA HPRKESQVRQ 1090 1100 1110 1120 1130 1140 LAWVGDGVWV SIRLDSTLRL YHAHTYQHLQ DVDIEPYVSK MLGTGKLGFS FVRITALMVS 1150 1160 1170 1180 1190 1200 CNRLWVGTGN GVIISIPLTE TNKTSGTPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH 1210 1220 1230 1240 1250 1260 AQLCFHGHRD AVKFFVAVPG QVISPQSSSG GADLTADKAG SSAQEPSSQT PLKSMLVISG 1270 1280 1290 1300 GEGYIDFRMG DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMCGNE
	Isoform 3 [UniParc]. Checksum: 34B7E6B2D81B7DF9	FASTA	1,168	127,680
	10 20 30 40 50 60 MNPGCMLLFV FGFVGGAVVI NSAILVSLSV LLLVHFSIST GVPALTQNLP RILRKERPIS 70 80 90 100 110 120 LGIFPLPAGD GLLTPDTQKG GETPGSEQWK FQELSQPRSH TSLKDELSDI SQGGSKATTP 130 140 150 160 170 180 ASTANSDVSA IPPDTPSKED NEGFVKGTDT SNKSEISKHI EVQVAQETRN VSTESGENEE 190 200 210 220 230 240 KSEVQAIIES TPELDMDKDL SGYKGSSTPT KGIENKAFDR NTESLFEELS SAGSGLIGDV 250 260 270 280 290 300 DEGADLLGEY SGMGREVENL ILENTQLLET KNALNVVKND LIAKVDELTC EKDVLQGELE 310 320 330 340 350 360 AVKQAKLKLE DKNRELEEEL RKARAEAEDA RQKAKDDDDS DIPTAQRKRF TRVEMARVLM 370 380 390 400 410 420 ERNQYKERLM ELQEAVRWTE MIRASRENPA MQEKKRSSIW QFFSRLFSSS SNATKKPEPP 430 440 450 460 470 480 VNLKYNAPTS HVTPSVKKRS STLSQLPGDK SKAFDFLSEE TEASLASRRE QKREQYRQVK 490 500 510 520 530 540 AHVQKEDGRV QAFGWSLPQK YKQVANGQGE TKMKNLPVPV YLRPLDEKDA SMKLWCAVGV 550 560 570 580 590 600 NLSGGKTRDG GSVVGASVFY KDIAGLDTEG SKQRSASQSS LDKLDQELKE QQKEFKNQEE 610 620 630 640 650 660 LSSQVWICTS THSTTKVIII DAVQPGNILD SFTVCNSHVL CIASVPGARE TDYPAGEELS 670 680 690 700 710 720 ESGQVDKASL CGSMTSNSSA EMDSLLGGIT VVGCSTEGLT GAATSPSTNG ASPVIEKPPE 730 740 750 760 770 780 METENSEVDE NIPTAEEATE ATEGNAGSTE DTVDISQPGV YTEHVFTDPL GVQIPEDLSP 790 800 810 820 830 840 VFQSSNDSDV YKDQISVLPN EQDLAREEAQ KMSSLLPTMW LGAQNGCLYV HSSVAQWRKC 850 860 870 880 890 900 LHSIKLKDSI LSIVHVKGIV LVALADGTLA IFHRGVDGQW DLSNYHLLDL GRPHHSIRCM 910 920 930 940 950 960 TVVHDKVWCG YRNKIYVVQP KAMKIEKSFD AHPRKESQVR QLAWVGDGVW VSIRLDSTLR 970 980 990 1000 1010 1020 LYHAHTYQHL QDVDIEPYVS KMLGTGKLGF SFVRITALMV SCNRLWVGTG NGVIISIPLT 1030 1040 1050 1060 1070 1080 ETNKTSGTPG NRPGSVIRVY GDENSDKVTP GTFIPYCSMA HAQLCFHGHR DAVKFFVAVP 1090 1100 1110 1120 1130 1140 GQVISPQSSS GGADLTADKA GSSAQEPSSQ TPLKSMLVIS GGEGYIDFRM GDEGGESELL 1150 1160 GEDLPLEPSV TKAERSHLIV WQVMCGNE
	Isoform 4 [UniParc]. Checksum: 730D991D29C5A74B	FASTA	1,177	128,672
	10 20 30 40 50 60 MNPGCMLLFV FGFVGGAVVI NSAILVSLSV LLLVHFSIST GVPALTQNLP RILRKERPIS 70 80 90 100 110 120 LGIFPLPAGD GLLTPDTQKG GETPGSEQWK FQELSQPRSH TSLKDELSDI SQGGSKATTP 130 140 150 160 170 180 ASTANSDVSA IPPDTPSKED NEGFVKGTDT SNKSEISKHI EVQVAQETRN VSTESGENEE 190 200 210 220 230 240 KSEVQAIIES TPELDMDKDL SGYKGSSTPT KGIENKAFDR NTESLFEELS SAGSGLIGDV 250 260 270 280 290 300 DEGADLLGMG REVENLILEN TQLLETKNAL NVVKNDLIAK VDELTCEKDV LQGELEAVKQ 310 320 330 340 350 360 AKLKLEDKNR ELEEELRKAR AEAEDARQKA KDDDDSDIPT AQRKRFTRVE MARVLMERNQ 370 380 390 400 410 420 YKERLMELQE AVRWTEMIRA SRENPAMQEK KRSSIWQFFS RLFSSSSNAT KKPEPPVNLK 430 440 450 460 470 480 YNAPTSHVTP SVKKRSSTLS QLPGDKSKAF DFLSEETEAS LASRREQKRE QYRQVKAHVQ 490 500 510 520 530 540 KEDGRVQAFG WSLPQKYKQV ANGQGETKMK NLPVPVYLRP LDEKDASMKL WCAVGVNLSG 550 560 570 580 590 600 GKTRDGGSVV GASVFYKDIA GLDTEGSKQR SASQSSLDKL DQELKEQQKE FKNQEELSSQ 610 620 630 640 650 660 VWICTSTHST TKVIIIDAVQ PGNILDSFTV CNSHVLCIAS VPGARETDYP AGEELSESGQ 670 680 690 700 710 720 VDKASLCGSM TSNSSAEMDS LLGGITVVGC STEGLTGAAT SPSTNGASPV IEKPPEMETE 730 740 750 760 770 780 NSEVDENIPT AEEATEATEG NAGSTEDTVD ISQPGVYTEH VFTDPLGVQI PEDLSPVFQS 790 800 810 820 830 840 SNDSDVYKDQ ISVLPNEQDL AREEAQKMSS LLPTMWLGAQ NGCLYVHSSV AQWRKCLHSI 850 860 870 880 890 900 KLKDSILSIV HVKGIVLVAL ADGTLAIFHR GVDGQWDLSN YHLLDLGRPH HSIRCMTVVH 910 920 930 940 950 960 DKVWCGYRNK IYVVQPKAMK IEKSFDAHPR KESQVRQLAW VGDGVWVSIR LDSTLRLYHA 970 980 990 1000 1010 1020 HTYQHLQDVD IEPYVSKMLG TGKLGFSFVR ITALMVSCNR LWVGTGNGVI ISIPLTETVI 1030 1040 1050 1060 1070 1080 LHQGRLLGLR ANKTSGTPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH AQLCFHGHRD 1090 1100 1110 1120 1130 1140 AVKFFVAVPG QVISPQSSSG GADLTADKAG SSAQEPSSQT PLKSMLVISG GEGYIDFRMG 1150 1160 1170 DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMCGNE
	Isoform 5 [UniParc]. Checksum: F186D445467EE5B6	FASTA	1,307	143,835
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISMNPGCML LFVFGFVGGA VVINSAILVS LSVLLLVHFS 190 200 210 220 230 240 ISTGVPALTQ NLPRILRKER PISLGIFPLP AGDGLLTPDT QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDISQGGSKA TTPASTANSD VSAIPPDTPS KEDNEGFVKG TDTSNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTESGE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNVVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLED KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FFSRLFSSSS NATKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 610 620 630 640 650 660 EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVANGQGET KMKNLPVPVY 670 680 690 700 710 720 LRPLDEKDAS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DIAGLDTEGS KQRSASQSSL 730 740 750 760 770 780 DKLDQELKEQ QKEFKNQEEL SSQVWICTST HSTTKVIIID AVQPGNILDS FTVCNSHVLC 790 800 810 820 830 840 IASVPGARET DYPAGEELSE SGQVDKASLC GSMTSNSSAE MDSLLGGITV VGCSTEGLTG 850 860 870 880 890 900 AATSPSTNGA SPVIEKPPEM ETENSEVDEN IPTAEEATEA TEGNAGSTED TVDISQPGVY 910 920 930 940 950 960 TEHVFTDPLG VQIPEDLSPV FQSSNDSDVY KDQISVLPNE QDLAREEAQK MSSLLPTMWL 970 980 990 1000 1010 1020 GAQNGCLYVH SSVAQWRKCL HSIKLKDSIL SIVHVKGIVL VALADGTLAI FHRGVDGQWD 1030 1040 1050 1060 1070 1080 LSNYHLLDLG RPHHSIRCMT VVHDKVWCGY RNKIYVVQPK AMKIEKSFDA HPRKESQVRQ 1090 1100 1110 1120 1130 1140 LAWVGDGVWV SIRLDSTLRL YHAHTYQHLQ DVDIEPYVSK MLGTGKLGFS FVRITALMVS 1150 1160 1170 1180 1190 1200 CNRLWVGTGN GVIISIPLTE TNKTSGTPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH 1210 1220 1230 1240 1250 1260 AQLCFHGHRD AVKFFVAVPG QVISPQSSSG GADLTADKAG SSAQEPSSQT PLKSMLVISG 1270 1280 1290 1300 GEGYIDFRMG DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMCGNE
	Isoform 6 [UniParc]. Checksum: 16184E1CE2BF26A1	FASTA	1,142	125,350
	10 20 30 40 50 60 MIHNYMEHLE RTKLHQLSGS DQLEATAHSR IRKERPISLG IFPLPAGDGL LTPDTQKGGE 70 80 90 100 110 120 TPGSEQWKFQ ELSQPRSHTS LKDELSDISQ GGSKATTPAS TANSDVSAIP PDTPSKEDNE 130 140 150 160 170 180 GFVKGTDTSN KSEISKHIEV QVAQETRNVS TESGENEEKS EVQAIIESTP ELDMDKDLSG 190 200 210 220 230 240 YKGSSTPTKG IENKAFDRNT ESLFEELSSA GSGLIGDVDE GADLLGMGRE VENLILENTQ 250 260 270 280 290 300 LLETKNALNV VKNDLIAKVD ELTCEKDVLQ GELEAVKQAK LKLEDKNREL EEELRKARAE 310 320 330 340 350 360 AEDARQKAKD DDDSDIPTAQ RKRFTRVEMA RVLMERNQYK ERLMELQEAV RWTEMIRASR 370 380 390 400 410 420 ENPAMQEKKR SSIWQFFSRL FSSSSNATKK PEPPVNLKYN APTSHVTPSV KKRSSTLSQL 430 440 450 460 470 480 PGDKSKAFDF LSEETEASLA SRREQKREQY RQVKAHVQKE DGRVQAFGWS LPQKYKQVAN 490 500 510 520 530 540 GQGETKMKNL PVPVYLRPLD EKDASMKLWC AVGVNLSGGK TRDGGSVVGA SVFYKDIAGL 550 560 570 580 590 600 DTEGSKQRSA SQSSLDKLDQ ELKEQQKEFK NQEELSSQVW ICTSTHSTTK VIIIDAVQPG 610 620 630 640 650 660 NILDSFTVCN SHVLCIASVP GARETDYPAG EELSESGQVD KASLCGSMTS NSSAEMDSLL 670 680 690 700 710 720 GGITVVGCST EGLTGAATSP STNGASPVIE KPPEMETENS EVDENIPTAE EATEATEGNA 730 740 750 760 770 780 GSTEDTVDIS QPGVYTEHVF TDPLGVQIPE DLSPVFQSSN DSDVYKDQIS VLPNEQDLAR 790 800 810 820 830 840 EEAQKMSSLL PTMWLGAQNG CLYVHSSVAQ WRKCLHSIKL KDSILSIVHV KGIVLVALAD 850 860 870 880 890 900 GTLAIFHRGV DGQWDLSNYH LLDLGRPHHS IRCMTVVHDK VWCGYRNKIY VVQPKAMKIE 910 920 930 940 950 960 KSFDAHPRKE SQVRQLAWVG DGVWVSIRLD STLRLYHAHT YQHLQDVDIE PYVSKMLGTG 970 980 990 1000 1010 1020 KLGFSFVRIT ALMVSCNRLW VGTGNGVIIS IPLTETNKTS GTPGNRPGSV IRVYGDENSD 1030 1040 1050 1060 1070 1080 KVTPGTFIPY CSMAHAQLCF HGHRDAVKFF VAVPGQVISP QSSSGGADLT ADKAGSSAQE 1090 1100 1110 1120 1130 1140 PSSQTPLKSM LVISGGEGYI DFRMGDEGGE SELLGEDLPL EPSVTKAERS HLIVWQVMCG NE

References

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[1]	"JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors." Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P. Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH MAX; MAPK8; MAPK14; MAP3K3; MYC AND MAP2K4, SUBCELLULAR LOCATION.
[2]	"Role of the JIP4 scaffold protein in the regulation of mitogen-activated protein kinase signaling pathways." Kelkar N., Standen C.L., Davis R.J. Mol. Cell. Biol. 25:2733-2743(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), FUNCTION, INTERACTION WITH KNS2 AND MAPK8, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION. Strain: C57BL/6J. Tissue: Testis.
[3]	"JSAP2, a novel member of the JSAP1 JNK scaffold protein family." Takamatsu N., Tochigi M., Ito M., Odama Y., Xu P., Nakabeppu Y., Yoshioka K., Shiba T. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Strain: C57BL/6J.
[6]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: C57BL/6J.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Strain: C57BL/6. Tissue: Brain and Olfactory epithelium.
[8]	"JLP associates with kinesin light chain 1 through a novel leucine zipper-like domain." Nguyen Q., Lee C.M., Le A., Reddy E.P. J. Biol. Chem. 280:30185-30191(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KNS2, INDUCTION.
[9]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-730 AND SER-733, MASS SPECTROMETRY. Tissue: Teratocarcinoma.
[10]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-244; THR-292 AND SER-733, MASS SPECTROMETRY. Tissue: Liver.
[11]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203 AND SER-730, MASS SPECTROMETRY. Tissue: Melanoma.
[12]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-217 AND THR-365, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF327451 mRNA. Translation: AAN61564.1. AY823270 mRNA. Translation: AAX19462.1. AB047782 mRNA. Translation: BAD93176.1. AK172961 mRNA. Translation: BAD32239.1. Different initiation. AK147431 mRNA. Translation: BAE27907.1. AK147537 mRNA. Translation: BAE27980.1. AL662838 Genomic DNA. Translation: CAI35367.1. AL662838 Genomic DNA. Translation: CAI35375.1. AL662838 Genomic DNA. Translation: CAI35376.1. Sequence problems. BC060100 mRNA. Translation: AAH60100.1. BC094670 mRNA. Translation: AAH94670.1.
IPI	IPI00462746. IPI00475034. IPI00626409. IPI00648688. IPI00753495. IPI00754112.
RefSeq	NP_001020599.1. NM_001025428.1. NP_001020600.1. NM_001025429.1. NP_001020601.1. NM_001025430.1. NP_001186132.1. NM_001199203.1. NP_001186133.1. NM_001199204.1. NP_001186134.1. NM_001199205.1. NP_081845.2. NM_027569.2.
UniGene	Mm.260737.
3D structure databases
ProteinModelPortal	Q58A65.
SMR	Q58A65. Positions 406-466.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q58A65. 1 interaction.
MINT	MINT-1351345.
STRING	10090.ENSMUSP00000099458.
PTM databases
PhosphoSite	Q58A65.
Proteomic databases
PaxDb	Q58A65.
PRIDE	Q58A65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041956; ENSMUSP00000042271; ENSMUSG00000020859. ENSMUST00000075695; ENSMUSP00000075115; ENSMUSG00000020859. ENSMUST00000103168; ENSMUSP00000099457; ENSMUSG00000020859.
GeneID	70834.
KEGG	mmu:70834.
UCSC	uc007kxx.1. mouse. uc007kxy.1. mouse. uc007kxz.1. mouse. uc007kya.1. mouse. uc007kyc.1. mouse. uc007kyd.1. mouse.
Organism-specific databases
CTD	9043.
MGI	MGI:1918084. Spag9.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG270333.
GeneTree	ENSGT00670000097546.
HOVERGEN	HBG024110.
InParanoid	Q58A65.
OMA	KKLWCAV.
OrthoDB	EOG4QJRMF.
Enzyme and pathway databases
Reactome	REACT_127416. Developmental Biology.
Gene expression databases
ArrayExpress	Q58A65.
Bgee	Q58A65.
CleanEx	MM_SPAG9.
Genevestigator	Q58A65.
GermOnline	ENSMUSG00000020859. Mus musculus.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR019143. JNK/Rab-associated_protein-1_N. IPR011044. Quino_amine_DH_bsu. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Pfam	PF09744. Jnk-SapK_ap_N. 1 hit. [Graphical view]
SUPFAM	SSF50969. Amine_DH_B_like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SPAG9. mouse.
NextBio	332372.
SOURCE	Search...

Entry information

Entry name

JIP4_MOUSE

Accession

Primary (citable) accession number: Q58A65
Secondary accession number(s): Q3UH77 , Q3UHF0, Q58VQ4, Q5NC70, Q5NC78, Q6A057, Q6PAS3, Q8CJC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	May 29, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program