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Protein

C-Jun-amino-terminal kinase-interacting protein 4

Gene

Spag9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module.2 Publications

GO - Molecular functioni

  • JUN kinase binding Source: MGI
  • kinesin binding Source: MGI
  • MAP-kinase scaffold activity Source: GO_Central
  • mitogen-activated protein kinase p38 binding Source: MGI
  • receptor signaling complex scaffold activity Source: GO_Central

GO - Biological processi

  • activation of JUN kinase activity Source: MGI
  • activation of MAPK activity Source: MGI
  • negative regulation of protein homodimerization activity Source: MGI
  • positive regulation of cell migration Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of muscle cell differentiation Source: Reactome
  • positive regulation of neuron differentiation Source: MGI
  • protein homooligomerization Source: MGI
  • retrograde transport, endosome to Golgi Source: MGI
  • striated muscle cell differentiation Source: MGI
  • vesicle-mediated transport Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-375170. CDO in myogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
C-Jun-amino-terminal kinase-interacting protein 4
Short name:
JIP-4
Short name:
JNK-interacting protein 4
Alternative name(s):
JNK-associated leucine-zipper protein
Short name:
JLP
JNK/SAPK-associated protein 2
Short name:
JSAP2
Mitogen-activated protein kinase 8-interacting protein 4
Sperm-associated antigen 9
Gene namesi
Name:Spag9
Synonyms:Jip4, Jsap2, Kiaa0516, Mapk8ip4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1918084. Spag9.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • extracellular exosome Source: MGI
  • microtubule organizing center Source: MGI
  • perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340771 – 1321C-Jun-amino-terminal kinase-interacting protein 4Add BLAST1321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei194PhosphoserineBy similarity1
Modified residuei203PhosphoserineCombined sources1
Modified residuei217PhosphothreonineCombined sources1
Modified residuei238PhosphoserineBy similarity1
Modified residuei251PhosphoserineBy similarity1
Modified residuei265PhosphoserineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1
Modified residuei292PhosphothreonineCombined sources1
Modified residuei311PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Modified residuei347PhosphoserineBy similarity1
Modified residuei348PhosphothreonineBy similarity1
Modified residuei365PhosphothreonineCombined sources1
Modified residuei418PhosphothreonineBy similarity1
Modified residuei586PhosphothreonineBy similarity1
Modified residuei588PhosphoserineBy similarity1
Modified residuei595PhosphothreonineBy similarity1
Modified residuei705PhosphoserineBy similarity1
Modified residuei728PhosphoserineBy similarity1
Modified residuei730PhosphoserineBy similarity1
Modified residuei732PhosphoserineBy similarity1
Modified residuei733PhosphoserineCombined sources1
Modified residuei1188PhosphoserineBy similarity1
Modified residuei1264PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated by MAPK8 and MAPK14.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ58A65.
PeptideAtlasiQ58A65.
PRIDEiQ58A65.

PTM databases

iPTMnetiQ58A65.
PhosphoSitePlusiQ58A65.

Expressioni

Tissue specificityi

Isoform 6 is highly expressed in brain, kidney, liver, heart.1 Publication

Inductioni

Up-regulated during neuronal differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000020859.
CleanExiMM_SPAG9.
ExpressionAtlasiQ58A65. baseline and differential.
GenevisibleiQ58A65. MM.

Interactioni

Subunit structurei

Homodimer. The homodimer interacts with ARF6, forming a heterotetramer (By similarity). Homooligomer. Interacts with MAX, MAPK8, MAPK14, MAPK10, MAPK14, MAP3K3, MYC, KNS2, and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arf6P6233110EBI-6530207,EBI-988682
LRRK2Q5S0072EBI-6530207,EBI-5323863From a different organism.

GO - Molecular functioni

  • JUN kinase binding Source: MGI
  • kinesin binding Source: MGI
  • MAP-kinase scaffold activity Source: GO_Central
  • mitogen-activated protein kinase p38 binding Source: MGI
  • receptor signaling complex scaffold activity Source: GO_Central

Protein-protein interaction databases

BioGridi214283. 5 interactors.
IntActiQ58A65. 3 interactors.
MINTiMINT-1351345.
STRINGi10090.ENSMUSP00000042271.

Structurei

3D structure databases

ProteinModelPortaliQ58A65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 95RH1PROSITE-ProRule annotationAdd BLAST89
Domaini500 – 604RH2PROSITE-ProRule annotationAdd BLAST105

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili66 – 166Sequence analysisAdd BLAST101
Coiled coili408 – 534By similarityAdd BLAST127
Coiled coili724 – 758Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the JIP scaffold family.Curated
Contains 1 RH1 domain.PROSITE-ProRule annotation
Contains 1 RH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2077. Eukaryota.
ENOG410XQ19. LUCA.
GeneTreeiENSGT00670000097546.
HOVERGENiHBG024110.
InParanoidiQ58A65.
KOiK20317.
OMAiPPVNVKY.
OrthoDBiEOG091G016S.
PhylomeDBiQ58A65.
TreeFamiTF313096.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR032486. JIP_LZII.
IPR019143. JNK/Rab-associated_protein-1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF16471. JIP_LZII. 1 hit.
PF09744. Jnk-SapK_ap_N. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51776. RH1. 1 hit.
PS51777. RH2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q58A65-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL
60 70 80 90 100
MPLVVAVLEN LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE
110 120 130 140 150
EKFIEFEDSQ EQEKKDLQTR VESLESQTRQ LELKAKNYAD QISRLEEREA
160 170 180 190 200
ELKKEYNALH QRHTEMIHNY MEHLERTKLH QLSGSDQLEA TAHSRIRKER
210 220 230 240 250
PISLGIFPLP AGDGLLTPDT QKGGETPGSE QWKFQELSQP RSHTSLKVSH
260 270 280 290 300
SPEPPKAVEQ EDELSDISQG GSKATTPAST ANSDVSAIPP DTPSKEDNEG
310 320 330 340 350
FVKGTDTSNK SEISKHIEVQ VAQETRNVST ESGENEEKSE VQAIIESTPE
360 370 380 390 400
LDMDKDLSGY KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG
410 420 430 440 450
ADLLGMGREV ENLILENTQL LETKNALNVV KNDLIAKVDE LTCEKDVLQG
460 470 480 490 500
ELEAVKQAKL KLEDKNRELE EELRKARAEA EDARQKAKDD DDSDIPTAQR
510 520 530 540 550
KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE NPAMQEKKRS
560 570 580 590 600
SIWQFFSRLF SSSSNATKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP
610 620 630 640 650
GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL
660 670 680 690 700
PQKYKQVANG QGETKMKNLP VPVYLRPLDE KDASMKLWCA VGVNLSGGKT
710 720 730 740 750
RDGGSVVGAS VFYKDIAGLD TEGSKQRSAS QSSLDKLDQE LKEQQKEFKN
760 770 780 790 800
QEELSSQVWI CTSTHSTTKV IIIDAVQPGN ILDSFTVCNS HVLCIASVPG
810 820 830 840 850
ARETDYPAGE ELSESGQVDK ASLCGSMTSN SSAEMDSLLG GITVVGCSTE
860 870 880 890 900
GLTGAATSPS TNGASPVIEK PPEMETENSE VDENIPTAEE ATEATEGNAG
910 920 930 940 950
STEDTVDISQ PGVYTEHVFT DPLGVQIPED LSPVFQSSND SDVYKDQISV
960 970 980 990 1000
LPNEQDLARE EAQKMSSLLP TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK
1010 1020 1030 1040 1050
DSILSIVHVK GIVLVALADG TLAIFHRGVD GQWDLSNYHL LDLGRPHHSI
1060 1070 1080 1090 1100
RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK SFDAHPRKES QVRQLAWVGD
1110 1120 1130 1140 1150
GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK LGFSFVRITA
1160 1170 1180 1190 1200
LMVSCNRLWV GTGNGVIISI PLTETNKTSG TPGNRPGSVI RVYGDENSDK
1210 1220 1230 1240 1250
VTPGTFIPYC SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSGGADLTA
1260 1270 1280 1290 1300
DKAGSSAQEP SSQTPLKSML VISGGEGYID FRMGDEGGES ELLGEDLPLE
1310 1320
PSVTKAERSH LIVWQVMCGN E
Length:1,321
Mass (Da):146,219
Last modified:May 2, 2006 - v2
Checksum:i93A6E97A62E0E92C
GO
Isoform 2 (identifier: Q58A65-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.

Show »
Length:1,307
Mass (Da):144,704
Checksum:i8C99195A5AFFC7BF
GO
Isoform 3 (identifier: Q58A65-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.
     144-196: RLEEREAELK...QLEATAHSRI → MNPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     405-405: G → GEYSG

Note: No experimental confirmation available.
Show »
Length:1,168
Mass (Da):127,680
Checksum:i34B7E6B2D81B7DF9
GO
Isoform 4 (identifier: Q58A65-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.
     144-196: RLEEREAELK...QLEATAHSRI → MNPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     1175-1175: T → TVILHQGRLLGLRA

Note: No experimental confirmation available.
Show »
Length:1,177
Mass (Da):128,672
Checksum:i730D991D29C5A74B
GO
Isoform 5 (identifier: Q58A65-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-196: RLEEREAELK...QLEATAHSRI → MNPGCMLLFV...ALTQNLPRIL
     248-261: Missing.

Note: No experimental confirmation available.
Show »
Length:1,307
Mass (Da):143,835
Checksum:iF186D445467EE5B6
GO
Isoform 6 (identifier: Q58A65-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-165: Missing.
     248-261: Missing.

Show »
Length:1,142
Mass (Da):125,350
Checksum:i16184E1CE2BF26A1
GO

Sequence cautioni

The sequence BAD32239 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI35376 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti241R → C in AAN61564 (PubMed:12391307).Curated1
Sequence conflicti453E → A in AAN61564 (PubMed:12391307).Curated1
Sequence conflicti468E → G in BAE27980 (PubMed:16141072).Curated1
Sequence conflicti705S → T in BAD93176 (Ref. 3) Curated1
Sequence conflicti1114L → P in BAE27907 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0182251 – 165Missing in isoform 6. 1 PublicationAdd BLAST165
Alternative sequenceiVSP_0182261 – 143Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST143
Alternative sequenceiVSP_018227144 – 196RLEER…AHSRI → MNPGCMLLFVFGFVGGAVVI NSAILVSLSVLLLVHFSIST GVPALTQNLPRIL in isoform 3, isoform 4 and isoform 5. 3 PublicationsAdd BLAST53
Alternative sequenceiVSP_018228248 – 261Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6. 5 PublicationsAdd BLAST14
Alternative sequenceiVSP_018229405G → GEYSG in isoform 3. 1 Publication1
Alternative sequenceiVSP_0182301175T → TVILHQGRLLGLRA in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327451 mRNA. Translation: AAN61564.1.
AY823270 mRNA. Translation: AAX19462.1.
AB047782 mRNA. Translation: BAD93176.1.
AK172961 mRNA. Translation: BAD32239.1. Different initiation.
AK147431 mRNA. Translation: BAE27907.1.
AK147537 mRNA. Translation: BAE27980.1.
AL662838 Genomic DNA. Translation: CAI35367.1.
AL662838 Genomic DNA. Translation: CAI35375.1.
AL662838 Genomic DNA. Translation: CAI35376.1. Sequence problems.
BC060100 mRNA. Translation: AAH60100.1.
BC094670 mRNA. Translation: AAH94670.1.
CCDSiCCDS25248.1. [Q58A65-2]
CCDS56797.1. [Q58A65-3]
RefSeqiNP_001020599.1. NM_001025428.1.
NP_001020600.1. NM_001025429.1. [Q58A65-3]
NP_001020601.1. NM_001025430.1.
NP_001186132.1. NM_001199203.1.
NP_001186133.1. NM_001199204.1.
NP_001186134.1. NM_001199205.1. [Q58A65-4]
NP_081845.2. NM_027569.2. [Q58A65-2]
XP_006534282.1. XM_006534219.2. [Q58A65-6]
UniGeneiMm.260737.
Mm.479010.

Genome annotation databases

EnsembliENSMUST00000041956; ENSMUSP00000042271; ENSMUSG00000020859. [Q58A65-2]
ENSMUST00000075695; ENSMUSP00000075115; ENSMUSG00000020859. [Q58A65-3]
GeneIDi70834.
KEGGimmu:70834.
UCSCiuc007kxx.1. mouse. [Q58A65-2]
uc007kxy.1. mouse. [Q58A65-1]
uc007kxz.1. mouse. [Q58A65-6]
uc007kya.1. mouse. [Q58A65-3]
uc007kyc.1. mouse. [Q58A65-5]
uc007kyd.1. mouse. [Q58A65-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327451 mRNA. Translation: AAN61564.1.
AY823270 mRNA. Translation: AAX19462.1.
AB047782 mRNA. Translation: BAD93176.1.
AK172961 mRNA. Translation: BAD32239.1. Different initiation.
AK147431 mRNA. Translation: BAE27907.1.
AK147537 mRNA. Translation: BAE27980.1.
AL662838 Genomic DNA. Translation: CAI35367.1.
AL662838 Genomic DNA. Translation: CAI35375.1.
AL662838 Genomic DNA. Translation: CAI35376.1. Sequence problems.
BC060100 mRNA. Translation: AAH60100.1.
BC094670 mRNA. Translation: AAH94670.1.
CCDSiCCDS25248.1. [Q58A65-2]
CCDS56797.1. [Q58A65-3]
RefSeqiNP_001020599.1. NM_001025428.1.
NP_001020600.1. NM_001025429.1. [Q58A65-3]
NP_001020601.1. NM_001025430.1.
NP_001186132.1. NM_001199203.1.
NP_001186133.1. NM_001199204.1.
NP_001186134.1. NM_001199205.1. [Q58A65-4]
NP_081845.2. NM_027569.2. [Q58A65-2]
XP_006534282.1. XM_006534219.2. [Q58A65-6]
UniGeneiMm.260737.
Mm.479010.

3D structure databases

ProteinModelPortaliQ58A65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214283. 5 interactors.
IntActiQ58A65. 3 interactors.
MINTiMINT-1351345.
STRINGi10090.ENSMUSP00000042271.

PTM databases

iPTMnetiQ58A65.
PhosphoSitePlusiQ58A65.

Proteomic databases

PaxDbiQ58A65.
PeptideAtlasiQ58A65.
PRIDEiQ58A65.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041956; ENSMUSP00000042271; ENSMUSG00000020859. [Q58A65-2]
ENSMUST00000075695; ENSMUSP00000075115; ENSMUSG00000020859. [Q58A65-3]
GeneIDi70834.
KEGGimmu:70834.
UCSCiuc007kxx.1. mouse. [Q58A65-2]
uc007kxy.1. mouse. [Q58A65-1]
uc007kxz.1. mouse. [Q58A65-6]
uc007kya.1. mouse. [Q58A65-3]
uc007kyc.1. mouse. [Q58A65-5]
uc007kyd.1. mouse. [Q58A65-4]

Organism-specific databases

CTDi9043.
MGIiMGI:1918084. Spag9.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2077. Eukaryota.
ENOG410XQ19. LUCA.
GeneTreeiENSGT00670000097546.
HOVERGENiHBG024110.
InParanoidiQ58A65.
KOiK20317.
OMAiPPVNVKY.
OrthoDBiEOG091G016S.
PhylomeDBiQ58A65.
TreeFamiTF313096.

Enzyme and pathway databases

ReactomeiR-MMU-375170. CDO in myogenesis.

Miscellaneous databases

ChiTaRSiSpag9. mouse.
PROiQ58A65.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020859.
CleanExiMM_SPAG9.
ExpressionAtlasiQ58A65. baseline and differential.
GenevisibleiQ58A65. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR032486. JIP_LZII.
IPR019143. JNK/Rab-associated_protein-1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF16471. JIP_LZII. 1 hit.
PF09744. Jnk-SapK_ap_N. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51776. RH1. 1 hit.
PS51777. RH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJIP4_MOUSE
AccessioniPrimary (citable) accession number: Q58A65
Secondary accession number(s): Q3UH77
, Q3UHF0, Q58VQ4, Q5NC70, Q5NC78, Q6A057, Q6PAS3, Q8CJC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.