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Protein

PAB-dependent poly(A)-specific ribonuclease subunit PAN3

Gene

PAN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei521ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri43 – 71C3H1-typeUniRule annotationAdd BLAST29
Nucleotide bindingi570 – 575ATPUniRule annotation6
Nucleotide bindingi644 – 645ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000154143-MONOMER.
ReactomeiR-HSA-429947. Deadenylation of mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
PAB-dependent poly(A)-specific ribonuclease subunit PAN3UniRule annotation
Alternative name(s):
PAB1P-dependent poly(A)-nucleaseUniRule annotation
PAN deadenylation complex subunit 3UniRule annotation
Short name:
hPan3
Gene namesi
Name:PAN3UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:29991. PAN3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: GO_Central
  • cytosol Source: Reactome
  • PAN complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi203Y → A: Reduces interaction with polyadenylate-binding protein. 1 Publication1
Mutagenesisi293F → A: Reduces interaction with polyadenylate-binding protein. 2 Publications1

Organism-specific databases

DisGeNETi255967.
OpenTargetsiENSG00000152520.
PharmGKBiPA144596398.

Polymorphism and mutation databases

BioMutaiPAN3.
DMDMi341942213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805251 – 887PAB-dependent poly(A)-specific ribonuclease subunit PAN3Add BLAST887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphoserineCombined sources1
Modified residuei361PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ58A45.
MaxQBiQ58A45.
PaxDbiQ58A45.
PeptideAtlasiQ58A45.
PRIDEiQ58A45.

PTM databases

iPTMnetiQ58A45.
PhosphoSitePlusiQ58A45.

Expressioni

Gene expression databases

BgeeiENSG00000152520.
CleanExiHS_PAN3.
ExpressionAtlasiQ58A45. baseline and differential.

Organism-specific databases

HPAiHPA028817.

Interactioni

Subunit structurei

Homodimer. Interacts with the catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PABPC1 (via PABC domain), conferring substrate specificity of the enzyme complex. Interacts with the GW182 family proteins TNRC6A, TNRC6B and TNRC6C.UniRule annotation5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PABPC1P119404EBI-2513054,EBI-81531
PAN2Q504Q36EBI-2513054,EBI-1058976
TNRC6AQ8NDV72EBI-2513054,EBI-2269715
TNRC6BQ9UPQ94EBI-2513054,EBI-947158
TNRC6CQ9HCJ04EBI-2513054,EBI-6507625

Protein-protein interaction databases

BioGridi129129. 11 interactors.
DIPiDIP-53755N.
IntActiQ58A45. 9 interactors.
STRINGi9606.ENSP00000370345.

Structurei

3D structure databases

ProteinModelPortaliQ58A45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini492 – 754Protein kinaseUniRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 491Interaction with polyadenylate-binding protein1 PublicationAdd BLAST491
Regioni492 – 887Interaction with PAN2UniRule annotation2 PublicationsAdd BLAST396

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili756 – 777UniRule annotationAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi284 – 299PABPC-interacting motif-2 (PAM-2)UniRule annotation1 PublicationAdd BLAST16

Domaini

The protein kinase domain is predicted to be catalytically inactive. However it still binds ATP and ATP-binding is required for mRNA degradation.UniRule annotation

Sequence similaritiesi

Belongs to the protein kinase superfamily. PAN3 family.UniRule annotation
Contains 1 C3H1-type zinc finger.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri43 – 71C3H1-typeUniRule annotationAdd BLAST29

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG3741. Eukaryota.
ENOG410XQ42. LUCA.
GeneTreeiENSGT00390000001504.
HOVERGENiHBG058849.
InParanoidiQ58A45.
KOiK12572.
OMAiYYAKDKT.
OrthoDBiEOG091G04WA.
PhylomeDBiQ58A45.
TreeFamiTF105865.

Family and domain databases

Gene3Di4.10.1000.10. 1 hit.
HAMAPiMF_03181. PAN3. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR030844. PAN3.
IPR000719. Prot_kinase_dom.
IPR000571. Znf_CCCH.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF90229. SSF90229. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q58A45-1) [UniParc]FASTAAdd to basket
Also known as: Pan3b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSGGGLPPP SAAASPSSSS LAAAVAVVAP PGVGGVPGGA AVGVKLKYCR
60 70 80 90 100
YYAKDKTCFY GEECQFLHED PAAGAAPGLG LHSNSVPLAL AGAPVAGFPP
110 120 130 140 150
GAVAGGGAGP PPGPKKPDLG DPGTGAAAGG GGSSGGLDGP RLAIPGMDGG
160 170 180 190 200
ALTDTSLTDS YFSTSFIGVN GFGSPVETKY PLMQRMTNSS SSPSLLNDSA
210 220 230 240 250
KPYSAHDPLT SPASSLFNDF GALNISQRRK PRKYRLGMLE ERLVPMGSKA
260 270 280 290 300
RKAKNPIGCL ADRCKSGVPI NMVWWNRVTE NNLQTPNPTA SEFIPKGGST
310 320 330 340 350
SRLSNVSQSN MSAFSQVFSH PSMGSPATAG LAPGMSLSAG SSPLHSPKIT
360 370 380 390 400
PHTSPAPRRR SHTPNPASYM VPSSASTSVN NPVSQTPSSG QVIQKETVGG
410 420 430 440 450
TTYFYTDTTP APLTGMVFPN YHIYPPTAPH VAYMQPKANA PSFFMADELR
460 470 480 490 500
QELINRHLIT MAQIDQADMP AVPTEVDSYH SLFPLEPLPP PNRIQKSSNF
510 520 530 540 550
GYITSCYKAV NSKDDLPYCL RRIHGFRLVN TKCMVLVDMW KKIQHSNIVT
560 570 580 590 600
LREVFTTKAF AEPSLVFAYD FHAGGETMMS RHFNDPNADA YFTKRKWGQH
610 620 630 640 650
EGPLPRQHAG LLPESLIWAY IVQLSSALRT IHTAGLACRV MDPTKILITG
660 670 680 690 700
KTRLRVNCVG VFDVLTFDNS QNNNPLALMA QYQQADLISL GKVVLALACN
710 720 730 740 750
SLAGIQRENL QKAMELVTIN YSSDLKNLIL YLLTDQNRMR SVNDIMPMIG
760 770 780 790 800
ARFYTQLDAA QMRNDVIEED LAKEVQNGRL FRLLAKLGTI NERPEFQKDP
810 820 830 840 850
TWSETGDRYL LKLFRDHLFH QVTEAGAPWI DLSHIISCLN KLDAGVPEKI
860 870 880
SLISRDEKSV LVVTYSDLKR CFENTFQELI AAANGQL
Length:887
Mass (Da):95,613
Last modified:July 27, 2011 - v3
Checksum:i4883F859D2A642D2
GO
Isoform 2 (identifier: Q58A45-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-332: Missing.
     333-333: P → MKLTDSTKGWIVWAALDSSMR

Show »
Length:575
Mass (Da):64,465
Checksum:i8FF7B393A072FD8D
GO
Isoform 3 (identifier: Q58A45-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-284: Missing.

Show »
Length:833
Mass (Da):89,405
Checksum:i06EAD23E1B205A4F
GO
Isoform 4 (identifier: Q58A45-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     684-687: QADL → VSKN
     688-887: Missing.

Show »
Length:687
Mass (Da):72,933
Checksum:iC3AFEB0807CE9F3B
GO

Sequence cautioni

The sequence AAI28180 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence ABK41888 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC03632 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI12449 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI12450 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI14848 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM20921 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti229R → Q in BAD02262 (PubMed:14583602).Curated1
Sequence conflicti569Y → H in AAI28181 (PubMed:15489334).Curated1
Sequence conflicti793R → G in BAC03632 (PubMed:14702039).Curated1
Sequence conflicti856D → G in BAD02262 (PubMed:14583602).Curated1
Sequence conflicti856D → G in BAD93184 (PubMed:14583602).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0237531 – 332Missing in isoform 2. 1 PublicationAdd BLAST332
Alternative sequenceiVSP_023754231 – 284Missing in isoform 3. 2 PublicationsAdd BLAST54
Alternative sequenceiVSP_023755333P → MKLTDSTKGWIVWAALDSSM R in isoform 2. 1 Publication1
Alternative sequenceiVSP_041648684 – 687QADL → VSKN in isoform 4. 1 Publication4
Alternative sequenceiVSP_041649688 – 887Missing in isoform 4. 1 PublicationAdd BLAST200

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK091307 mRNA. Translation: BAC03632.1. Different initiation.
AK296435 mRNA. Translation: BAH12353.1.
EF088216 Genomic DNA. Translation: ABK41888.1. Sequence problems.
AL356915, AL138712 Genomic DNA. Translation: CAI12449.2. Sequence problems.
AL356915, AL138712 Genomic DNA. Translation: CAI12450.2. Sequence problems.
AL138712, AL356915 Genomic DNA. Translation: CAI14848.1. Sequence problems.
AL138712, AL356915 Genomic DNA. Translation: CAM20921.1. Sequence problems.
BC024318 mRNA. Translation: AAH24318.1.
BC128179 mRNA. Translation: AAI28180.1. Different initiation.
BC128180 mRNA. Translation: AAI28181.1.
AB107584 mRNA. Translation: BAD02262.1.
AB109552 mRNA. Translation: BAD93184.1.
BX647740 mRNA. Translation: CAI45987.2.
CCDSiCCDS9329.2. [Q58A45-1]
RefSeqiNP_787050.6. NM_175854.7. [Q58A45-1]
XP_005266390.1. XM_005266333.3. [Q58A45-3]
XP_005266391.1. XM_005266334.1. [Q58A45-2]
XP_016876021.1. XM_017020532.1. [Q58A45-2]
XP_016876022.1. XM_017020533.1.
UniGeneiHs.645015.

Genome annotation databases

EnsembliENST00000380958; ENSP00000370345; ENSG00000152520. [Q58A45-1]
GeneIDi255967.
KEGGihsa:255967.
UCSCiuc001urz.4. human. [Q58A45-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK091307 mRNA. Translation: BAC03632.1. Different initiation.
AK296435 mRNA. Translation: BAH12353.1.
EF088216 Genomic DNA. Translation: ABK41888.1. Sequence problems.
AL356915, AL138712 Genomic DNA. Translation: CAI12449.2. Sequence problems.
AL356915, AL138712 Genomic DNA. Translation: CAI12450.2. Sequence problems.
AL138712, AL356915 Genomic DNA. Translation: CAI14848.1. Sequence problems.
AL138712, AL356915 Genomic DNA. Translation: CAM20921.1. Sequence problems.
BC024318 mRNA. Translation: AAH24318.1.
BC128179 mRNA. Translation: AAI28180.1. Different initiation.
BC128180 mRNA. Translation: AAI28181.1.
AB107584 mRNA. Translation: BAD02262.1.
AB109552 mRNA. Translation: BAD93184.1.
BX647740 mRNA. Translation: CAI45987.2.
CCDSiCCDS9329.2. [Q58A45-1]
RefSeqiNP_787050.6. NM_175854.7. [Q58A45-1]
XP_005266390.1. XM_005266333.3. [Q58A45-3]
XP_005266391.1. XM_005266334.1. [Q58A45-2]
XP_016876021.1. XM_017020532.1. [Q58A45-2]
XP_016876022.1. XM_017020533.1.
UniGeneiHs.645015.

3D structure databases

ProteinModelPortaliQ58A45.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129129. 11 interactors.
DIPiDIP-53755N.
IntActiQ58A45. 9 interactors.
STRINGi9606.ENSP00000370345.

PTM databases

iPTMnetiQ58A45.
PhosphoSitePlusiQ58A45.

Polymorphism and mutation databases

BioMutaiPAN3.
DMDMi341942213.

Proteomic databases

EPDiQ58A45.
MaxQBiQ58A45.
PaxDbiQ58A45.
PeptideAtlasiQ58A45.
PRIDEiQ58A45.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380958; ENSP00000370345; ENSG00000152520. [Q58A45-1]
GeneIDi255967.
KEGGihsa:255967.
UCSCiuc001urz.4. human. [Q58A45-1]

Organism-specific databases

CTDi255967.
DisGeNETi255967.
GeneCardsiPAN3.
H-InvDBHIX0011200.
HGNCiHGNC:29991. PAN3.
HPAiHPA028817.
neXtProtiNX_Q58A45.
OpenTargetsiENSG00000152520.
PharmGKBiPA144596398.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3741. Eukaryota.
ENOG410XQ42. LUCA.
GeneTreeiENSGT00390000001504.
HOVERGENiHBG058849.
InParanoidiQ58A45.
KOiK12572.
OMAiYYAKDKT.
OrthoDBiEOG091G04WA.
PhylomeDBiQ58A45.
TreeFamiTF105865.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000154143-MONOMER.
ReactomeiR-HSA-429947. Deadenylation of mRNA.

Miscellaneous databases

ChiTaRSiPAN3. human.
GenomeRNAii255967.
PROiQ58A45.

Gene expression databases

BgeeiENSG00000152520.
CleanExiHS_PAN3.
ExpressionAtlasiQ58A45. baseline and differential.

Family and domain databases

Gene3Di4.10.1000.10. 1 hit.
HAMAPiMF_03181. PAN3. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR030844. PAN3.
IPR000719. Prot_kinase_dom.
IPR000571. Znf_CCCH.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF90229. SSF90229. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAN3_HUMAN
AccessioniPrimary (citable) accession number: Q58A45
Secondary accession number(s): A0N0X1
, A1A4Y8, A1A4Y9, B1ALF1, B7Z3W7, Q0D2P2, Q5HYG6, Q5T515, Q5T516, Q5TBA0, Q76E13, Q8NBA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.