Q58999 (K6PF_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional ADP-specific glucokinase/phosphofructokinase
EC=2.7.1.146
EC=2.7.1.147

Alternative name(s):
ADP-GK
ADP-GK/PFK
ADP-Pfk
ADP-dependent glucokinase
ADP-dependent phosphofructokinase

Gene names

Name:	pfkC
Synonyms:	glkA
Ordered Locus Names:	MJ1604

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of fructose 6-phosphate and D-glucose to fructose 1,6-bisphosphate and D-glucose 6-phosphate, respectively, using ADP as the phosphate donor. HAMAP MF_00561
Catalytic activity	ADP + D-glucose = AMP + D-glucose 6-phosphate. HAMAP MF_00561 ADP + D-fructose 6-phosphate = AMP + D-fructose 1,6-bisphosphate. HAMAP MF_00561
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00561
Pathway	Carbohydrate degradation; glycolysis. HAMAP MF_00561
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP MF_00561.
Sequence similarities	Belongs to the carbohydrate kinase pfkC family. Contains 1 ADPK (ADP-dependent kinase) domain.
Biophysicochemical properties	Kinetic parameters: KM=1.6 mM for D-glucose Ref.3 KM=0.032 mM for ADP with D-glucose as phosphoryl group acceptor KM=9.6 µM for fructose 6-phosphate KM=0.49 mM for ADP with D-fructose 6-phosphate as phosphoryl group acceptor Vmax=11.2 µmol/min/mg enzyme toward fructose 6-phosphate Vmax=9.59 µmol/min/mg enzyme toward ADP pH dependence: Optimum pH is 6.5.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	fructose metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ADP-specific glucokinase activity Inferred from electronic annotation. Source: EC ADP-specific phosphofructokinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphofructokinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Bifunctional ADP-specific glucokinase/phosphofructokinase HAMAP MF_00561		PRO_0000184764
Regions
	Domain	1 – 458	458	ADPK
Sites
	Active site	442	1	Proton acceptor By similarity
	Metal binding	272	1	Magnesium By similarity
	Metal binding	302	1	Magnesium By similarity
	Metal binding	442	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58999 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D91088B60DBAB104	FASTA	462	53,362
	10 20 30 40 50 60 MCDIMEIKKF IETIKGTKLF TAYNTNVDAI KYLKDEDVQK LVDEFNHKDI IERMEEYPRI 70 80 90 100 110 120 IEEPLDFVAR LVHSIKTGKP AEVPIKDDKK LHEWFDRIKY DEERMGGQAG IVSNLMATLQ 130 140 150 160 170 180 IDKIIVYTPF LSKKQAEMFV DYDNLLYPLV ENGNLVLKKV REAYRDDPIK INRIFEFKKG 190 200 210 220 230 240 LKFKLNGEEI TAKQSTRFIV ASRPEALRIE IKDDVRKFLP KIGEAVDCAF LSGYQAIKEE 250 260 270 280 290 300 YRDGKTAKYY FERAEEDIKL LKKNKNIKTH LEFASISNIE IRKMVVDYIL SNVESVGMDE 310 320 330 340 350 360 TEIANVLHIL GYDELSNNIL KDSFIEDVIE GAKILLDKFK NLEVVQVHTI YYILFVCRAD 370 380 390 400 410 420 NPLSKEELEE CLEFSTILAS TKAKLGNIRA IDDLHEGLKI PHNKYGDLLK EIAEKFNDNN 430 440 450 460 YKIALSPSRY VEKPKSTVGL GDTISSGAFV YYVSLLNKKR MS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea." Verhees C.H., Tuininga J.E., Kengen S.W.M., Stams A.J.M., van der Oost J., de Vos W.M. J. Bacteriol. 183:7145-7153(2001) [PubMed: 11717273] [Abstract] Cited for: CHARACTERIZATION.
[3]	"ADP-dependent glucokinase/phosphofructokinase, a novel bifunctional enzyme from the hyperthermophilic archaeon Methanococcus jannaschii." Sakuraba H., Yoshioka I., Koga S., Takahashi M., Kitahama Y., Satomura T., Kawakami R., Ohshima T. J. Biol. Chem. 277:12495-12498(2002) [PubMed: 11856730] [Abstract] Cited for: CHARACTERIZATION, ENZYME KINETICS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99627.1.
PIR	C64500.
RefSeq	NP_248614.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58999.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452513.
GenomeReviews	Gene locus MJ1604 in contig L77117_GR.
KEGG	mja:MJ_1604.
NMPDR	fig|243232.1.peg.1651.
TIGR	MJ1604.
Phylogenomic databases
HOGENOM	HBG645711.
OMA	HLEFASI.
ProtClustDB	PRK03979.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1604-MONOMER.
Family and domain databases
HAMAP	MF_00561. ADP_PFKinase. [Tree]
InterPro	IPR007666. ADP_PFK/GK. IPR015990. ADP_PFK/GK_arc. IPR011790. ADP_PFK_arc. [Graphical view]
KO	K00918.
Pfam	PF04587. ADP_PFK_GK. 1 hit. [Graphical view]
PIRSF	PIRSF015883. ADP-Pfk_glckin. 1 hit.
TIGRFAMs	TIGR02045. P_fruct_ADP. 1 hit.
PROSITE	PS51255. ADPK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_METJA

Accession

Primary (citable) accession number: Q58999

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program