Q58987 (PUR7_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	MJ1592

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 242

242

Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137

PRO_0000100909

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

242

Helix Strand Turn

Details...

Helix

6 – 10

5

Beta strand

14 – 16

3

Beta strand

18 – 24

7

Beta strand

26 – 34

9

Beta strand

36 – 40

5

Beta strand

45 – 48

4

Helix

52 – 69

18

Beta strand

74 – 80

7

Turn

81 – 83

3

Beta strand

84 – 88

5

Beta strand

94 – 102

9

Helix

106 – 110

5

Beta strand

118 – 128

11

Turn

131 – 134

4

Helix

140 – 146

7

Helix

151 – 174

24

Beta strand

177 – 184

8

Beta strand

186 – 188

3

Beta strand

194 – 196

3

Turn

202 – 204

3

Beta strand

205 – 209

5

Turn

210 – 212

3

Helix

219 – 222

4

Helix

228 – 238

11

Sequences

	Sequence		Length	Mass (Da)
	Q58987 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E72A473335DA3E1E	FASTA	242	27,761
	10 20 30 40 50 60 MEIKLEEILK KQPLYSGKAK SIYEIDDDKV LIEFRDDITA GNGAKHDVKQ GKGYLNALIS 70 80 90 100 110 120 SKLFEALEEN GVKTHYIKYI EPRYMIAKKV EIIPIEVIVR NIAAGSLCRR YPFEEGKELP 130 140 150 160 170 180 FPIVQFDYKN DEYGDPMLNE DIAVALGLAT REELNKIKEI ALKVNEVLKK LFDEKGIILV 190 200 210 220 230 240 DFKIEIGKDR EGNLLVADEI SPDTMRLWDK ETRDVLDKDV FRKDLGDVIA KYRIVAERLG LL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB99610.1.

PIR

G64498.

RefSeq

NP_248601.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YZL	X-ray	2.20	A	1-242	[»]
2Z02	X-ray	2.03	A/B	1-242	[»]

ProteinModelPortal

Q58987.

SMR

Q58987. Positions 1-242.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1452500.

GenomeReviews

Gene locus MJ1592 in contig L77117_GR.

KEGG

mja:MJ_1592.

NMPDR

fig|243232.1.peg.1638.

TIGR

MJ1592.

Phylogenomic databases

HOGENOM

HBG306070.

OMA

YKDDALG.

ProtClustDB

PRK09362.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_1592-MONOMER.

Family and domain databases

HAMAP

MF_00137. SAICAR_synth.
[Tree]

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

KO

K01923.

PANTHER

PTHR11609. SAICAR_synt. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00081. PurC. 1 hit.

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PUR7_METJA

Accession

Primary (citable) accession number: Q58987

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program