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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi81PotassiumBy similarity1
Metal bindingi154PotassiumBy similarity1
Binding sitei192NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi195PotassiumBy similarity1
Binding sitei332NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei433NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi404 – 408ADPBy similarity5
Nucleotide bindingi423 – 432ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:MJ1586
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001190511 – 491Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST491

Proteomic databases

PRIDEiQ58981

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_1586

Structurei

3D structure databases

ProteinModelPortaliQ58981
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 230YjeF N-terminalAdd BLAST196
Domaini232 – 490YjeF C-terminalAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 230NAD(P)H-hydrate epimeraseBy similarityAdd BLAST230
Regioni80 – 84NAD(P)HX (for epimerase activity)By similarity5
Regioni158 – 164NAD(P)HX (for epimerase activity)By similarity7
Regioni232 – 491ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST260
Regioni380 – 386NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiarCOG00018 Archaea
ENOG4102TCZ Archaea
COG0062 LUCA
COG0063 LUCA
InParanoidiQ58981
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG093Z0AXM
PhylomeDBiQ58981

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q58981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRDINNFLF GERMELFEIL KQKIKEKEVI TPKEMAIIDD NAEFLGIQKI
60 70 80 90 100
LLMENAGKAV YEEIKDIDAE EFIIFCGTGN NGGDGFVVAR HLGKGDVILI
110 120 130 140 150
GKESEIKTYE ARENFKILKN LAEFGNIRIR EIKWAEEVND IFERLKNKKA
160 170 180 190 200
VIIDAMIGTG VKGELREPFK TIVDKINELK QINKNIFVIS VDVETGHLES
210 220 230 240 250
DLTITFHKRK TINKDNAIVK KIGIPKEAEY IVGWGDLKAL RKRDSNSHKG
260 270 280 290 300
QNGKVLIIGG SKDFYGAPIL AGLAALKIVD LVGILSVGKV IDKVNHPEFI
310 320 330 340 350
MYRVEGDYLS SQHVDYTLEI AKKYDVVVLG NGLGANNRTK AFLNEFLAKY
360 370 380 390 400
DGKVVIDADA IKVIDYNNFE FSENYIFTPH KREFEYMGID LDNIENIKST
410 420 430 440 450
IVLKGKYDII FNANNLKINK TGNAGLTKGG TGDVLAGLIG ALFAVNEAFL
460 470 480 490
SACCGAFING YAGDLLLKEK GYYYTPLDLI EKIPNVLKIF Q
Length:491
Mass (Da):54,979
Last modified:November 1, 1996 - v1
Checksum:i6F8994706685F6F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB99605.1
PIRiA64498

Genome annotation databases

EnsemblBacteriaiAAB99605; AAB99605; MJ_1586
KEGGimja:MJ_1586

Similar proteinsi

Entry informationi

Entry nameiNNR_METJA
AccessioniPrimary (citable) accession number: Q58981
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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