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Q58980

- DKFP_METJA

UniProt

Q58980 - DKFP_METJA

Protein

Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthase

Gene

MJ1585

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the transaldolisation of either fructose-1-P or fructose-1,6-bisphosphate with methylglyoxal to produce 6-deoxy-5-ketofructose-1-phosphate (DKFP). Also catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P or GAP) to produce fructose 1,6-bisphosphate (FBP).1 Publication

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.1 Publication
    Methylglyoxal + D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate.1 Publication
    Methylglyoxal + D-fructose 1-phosphate = D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate.1 Publication

    Pathwayi

    Aromatic compound metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531SubstrateBy similarity
    Binding sitei57 – 571SubstrateBy similarity
    Binding sitei180 – 1801SubstrateBy similarity
    Active sitei182 – 1821Proton donorBy similarity
    Binding sitei184 – 1841SubstrateBy similarity
    Active sitei213 – 2131Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Active sitei213 – 2131Schiff-base intermediate with substrateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB
    2. transferase activity, transferring aldehyde or ketonic groups Source: UniProtKB

    GO - Biological processi

    1. metabolic process Source: GOC

    Keywords - Molecular functioni

    Lyase, Transferase

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14590.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthase (EC:2.2.1.11, EC:4.1.2.13)
    Alternative name(s):
    DKFP synthase
    Fructose-bisphosphate aldolase class 1
    Fructose-bisphosphate aldolase class I
    Short name:
    FBP aldolase
    Gene namesi
    Ordered Locus Names:MJ1585
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthasePRO_0000138958Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243232.MJ1585.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58980.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 492Substrate bindingBy similarity
    Regioni213 – 2153Substrate bindingBy similarity
    Regioni241 – 2433Substrate bindingBy similarity
    Regioni270 – 2712Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DeoC/FbaB aldolase family.Curated

    Phylogenomic databases

    eggNOGiCOG1830.
    KOiK16305.
    OMAiKINSKTH.
    PhylomeDBiQ58980.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002915. DeoC/FbaB/lacD_aldolase.
    [Graphical view]
    PfamiPF01791. DeoC. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q58980-1 [UniParc]FASTAAdd to Basket

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    MGIFMIKRLK KRDVKVPLTV PEDRKEEYIK NYLELTKRTG NVMLFAGDQK    50
    IEHLNDDFFG EGIAKDDASP EHLFNIASKG KICGFATQLG LIARYGMDYK 100
    KIPYIVKINS KTHLVKTRDP ISRALVHVKD VVDLKENSGL KILGVGYTIY 150
    PGSEYEHIMF EEASRVILEA HKHGLIAIIW SYPRGKNVKD EKDPHLIAGA 200
    AGVAACLGAD FVKVNYPKCD NPAERFKEAV LAAGRTGVLC AGGKSIEPEK 250
    FLKQIWEQIN ISGARGNATG RNIHQKPLDA AIRMCNAIYA ITIEGKSLEE 300
    ALKIYYGDRK 310
    Length:310
    Mass (Da):34,574
    Last modified:November 1, 1996 - v1
    Checksum:i4AB6286F888049C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99604.1.
    PIRiH64497.
    RefSeqiNP_248594.1. NC_000909.1.
    WP_010871110.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99604; AAB99604; MJ_1585.
    GeneIDi1452494.
    KEGGimja:MJ_1585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99604.1 .
    PIRi H64497.
    RefSeqi NP_248594.1. NC_000909.1.
    WP_010871110.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58980.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1585.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99604 ; AAB99604 ; MJ_1585 .
    GeneIDi 1452494.
    KEGGi mja:MJ_1585.

    Phylogenomic databases

    eggNOGi COG1830.
    KOi K16305.
    OMAi KINSKTH.
    PhylomeDBi Q58980.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14590.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002915. DeoC/FbaB/lacD_aldolase.
    [Graphical view ]
    Pfami PF01791. DeoC. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038992. Aldolase_Ia. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii."
      White R.H., Xu H.
      Biochemistry 45:12366-12379(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A FRUCTOSE-BISPHOSPHATE ALDOLASE AND DKFP SYNTHASE, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiDKFP_METJA
    AccessioniPrimary (citable) accession number: Q58980
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3