Q58980 (DKFP_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthase
EC=2.2.1.11
EC=4.1.2.13

Alternative name(s):
DKFP synthase
Fructose-bisphosphate aldolase class 1
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase

Gene names

Ordered Locus Names:

MJ1585

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transaldolisation of either fructose-1-P or fructose-1,6-bisphosphate with methylglyoxal to produce 6-deoxy-5-ketofructose-1-phosphate (DKFP). Also catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P or GAP) to produce fructose 1,6-bisphosphate (FBP). Ref.2
Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. Ref.2 Methylglyoxal + D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate. Ref.2 Methylglyoxal + D-fructose 1-phosphate = D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate. Ref.2
Pathway	Aromatic compound metabolism.
Sequence similarities	Belongs to the DeoC/FbaB aldolase family.

Ontologies

Keywords
Ligand	Schiff base
Molecular function	Lyase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	fructose-bisphosphate aldolase activity Inferred from direct assay Ref.2. Source: UniProtKB transferase activity, transferring aldehyde or ketonic groups Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthase		PRO_0000138958
Regions
	Region	48 – 49	2	Substrate binding By similarity
	Region	213 – 215	3	Substrate binding By similarity
	Region	241 – 243	3	Substrate binding By similarity
	Region	270 – 271	2	Substrate binding By similarity
Sites
	Active site	182	1	Proton donor By similarity
	Active site	213	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Active site	213	1	Schiff-base intermediate with substrate By similarity
	Binding site	53	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58980 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4AB6286F888049C0	FASTA	310	34,574
	10 20 30 40 50 60 MGIFMIKRLK KRDVKVPLTV PEDRKEEYIK NYLELTKRTG NVMLFAGDQK IEHLNDDFFG 70 80 90 100 110 120 EGIAKDDASP EHLFNIASKG KICGFATQLG LIARYGMDYK KIPYIVKINS KTHLVKTRDP 130 140 150 160 170 180 ISRALVHVKD VVDLKENSGL KILGVGYTIY PGSEYEHIMF EEASRVILEA HKHGLIAIIW 190 200 210 220 230 240 SYPRGKNVKD EKDPHLIAGA AGVAACLGAD FVKVNYPKCD NPAERFKEAV LAAGRTGVLC 250 260 270 280 290 300 AGGKSIEPEK FLKQIWEQIN ISGARGNATG RNIHQKPLDA AIRMCNAIYA ITIEGKSLEE 310 ALKIYYGDRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii." White R.H., Xu H. Biochemistry 45:12366-12379(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A FRUCTOSE-BISPHOSPHATE ALDOLASE AND DKFP SYNTHASE, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99604.1.
PIR	H64497.
RefSeq	NP_248594.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58980.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ1585.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB99604; AAB99604; MJ_1585.
GeneID	1452494.
KEGG	mja:MJ_1585.
Phylogenomic databases
eggNOG	COG1830.
KO	K16305.
OMA	RNIHQRP.
ProtClustDB	PRK06852.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14590.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/FbaB/lacD_aldolase. [Graphical view]
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DKFP_METJA

Accession

Primary (citable) accession number: Q58980

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program