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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.1 Publication

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, N-terminal section (carB1), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain, C-terminal section (carB2)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BRENDAi2.1.3.2. 3260.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:MJ1581
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001132471 – 306Aspartate carbamoyltransferaseAdd BLAST306

Interactioni

Subunit structurei

Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2).1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1581.

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi12 – 30Combined sources19
Turni37 – 40Combined sources4
Beta strandi42 – 49Combined sources8
Helixi52 – 64Combined sources13
Beta strandi68 – 72Combined sources5
Turni76 – 79Combined sources4
Beta strandi80 – 83Combined sources4
Helixi86 – 96Combined sources11
Beta strandi98 – 103Combined sources6
Beta strandi105 – 108Combined sources4
Helixi109 – 116Combined sources8
Beta strandi121 – 124Combined sources4
Beta strandi126 – 129Combined sources4
Helixi132 – 146Combined sources15
Beta strandi149 – 151Combined sources3
Beta strandi153 – 158Combined sources6
Turni160 – 162Combined sources3
Helixi164 – 174Combined sources11
Beta strandi176 – 178Combined sources3
Beta strandi180 – 184Combined sources5
Helixi187 – 189Combined sources3
Helixi193 – 201Combined sources9
Beta strandi206 – 210Combined sources5
Helixi212 – 214Combined sources3
Beta strandi220 – 224Combined sources5
Helixi229 – 231Combined sources3
Helixi235 – 245Combined sources11
Helixi249 – 252Combined sources4
Beta strandi258 – 260Combined sources3
Beta strandi266 – 269Combined sources4
Helixi271 – 273Combined sources3
Helixi281 – 302Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RGWX-ray2.80A/B/C/D/E/F1-306[»]
3E2PX-ray3.00A/B/C/D/E/F/I/J/K/L/M/N1-306[»]
4EKNX-ray2.50B1-306[»]
ProteinModelPortaliQ58976.
SMRiQ58976.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58976.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiarCOG00911. Archaea.
COG0540. LUCA.
InParanoidiQ58976.
KOiK00609.
OMAiKQSFYGV.
PhylomeDBiQ58976.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHLISMKDI GKEEILEILD EARKMEELLN TKRPLKLLEG KILATVFYEP
60 70 80 90 100
STRTRLSFET AMKRLGGEVI TMTDLKSSSV AKGESLIDTI RVISGYADII
110 120 130 140 150
VLRHPSEGAA RLASEYSQVP IINAGDGSNQ HPTQTLLDLY TIMREIGRID
160 170 180 190 200
GIKIAFVGDL KYGRTVHSLV YALSLFENVE MYFVSPKELR LPKDIIEDLK
210 220 230 240 250
AKNIKFYEKE SLDDLDDDID VLYVTRIQKE RFPDPNEYEK VKGSYKIKRE
260 270 280 290 300
YVEGKKFIIM HPLPRVDEID YDVDDLPQAK YFKQSFYGIP VRMAILKKLI

EDNEGE
Length:306
Mass (Da):35,160
Last modified:November 1, 1996 - v1
Checksum:iCBDC31FC450CEF6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99601.1.
PIRiD64497.
RefSeqiWP_010871106.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99601; AAB99601; MJ_1581.
GeneIDi1452490.
KEGGimja:MJ_1581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99601.1.
PIRiD64497.
RefSeqiWP_010871106.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RGWX-ray2.80A/B/C/D/E/F1-306[»]
3E2PX-ray3.00A/B/C/D/E/F/I/J/K/L/M/N1-306[»]
4EKNX-ray2.50B1-306[»]
ProteinModelPortaliQ58976.
SMRiQ58976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99601; AAB99601; MJ_1581.
GeneIDi1452490.
KEGGimja:MJ_1581.

Phylogenomic databases

eggNOGiarCOG00911. Archaea.
COG0540. LUCA.
InParanoidiQ58976.
KOiK00609.
OMAiKQSFYGV.
PhylomeDBiQ58976.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BRENDAi2.1.3.2. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58976.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRB_METJA
AccessioniPrimary (citable) accession number: Q58976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.