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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.1 Publication

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, N-terminal section (carB1), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain, C-terminal section (carB2)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BRENDAi2.1.3.2. 3260.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:MJ1581
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Aspartate carbamoyltransferasePRO_0000113247Add
BLAST

Interactioni

Subunit structurei

Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2).1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_1581.

Structurei

Secondary structure

1
306
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Helixi12 – 3019Combined sources
Turni37 – 404Combined sources
Beta strandi42 – 498Combined sources
Helixi52 – 6413Combined sources
Beta strandi68 – 725Combined sources
Turni76 – 794Combined sources
Beta strandi80 – 834Combined sources
Helixi86 – 9611Combined sources
Beta strandi98 – 1036Combined sources
Beta strandi105 – 1084Combined sources
Helixi109 – 1168Combined sources
Beta strandi121 – 1244Combined sources
Beta strandi126 – 1294Combined sources
Helixi132 – 14615Combined sources
Beta strandi149 – 1513Combined sources
Beta strandi153 – 1586Combined sources
Turni160 – 1623Combined sources
Helixi164 – 17411Combined sources
Beta strandi176 – 1783Combined sources
Beta strandi180 – 1845Combined sources
Helixi187 – 1893Combined sources
Helixi193 – 2019Combined sources
Beta strandi206 – 2105Combined sources
Helixi212 – 2143Combined sources
Beta strandi220 – 2245Combined sources
Helixi229 – 2313Combined sources
Helixi235 – 24511Combined sources
Helixi249 – 2524Combined sources
Beta strandi258 – 2603Combined sources
Beta strandi266 – 2694Combined sources
Helixi271 – 2733Combined sources
Helixi281 – 30222Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RGWX-ray2.80A/B/C/D/E/F1-306[»]
3E2PX-ray3.00A/B/C/D/E/F/I/J/K/L/M/N1-306[»]
4EKNX-ray2.50B1-306[»]
ProteinModelPortaliQ58976.
SMRiQ58976. Positions 2-300.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58976.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiarCOG00911. Archaea.
COG0540. LUCA.
InParanoidiQ58976.
KOiK00609.
OMAiKQSFYGV.
PhylomeDBiQ58976.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHLISMKDI GKEEILEILD EARKMEELLN TKRPLKLLEG KILATVFYEP
60 70 80 90 100
STRTRLSFET AMKRLGGEVI TMTDLKSSSV AKGESLIDTI RVISGYADII
110 120 130 140 150
VLRHPSEGAA RLASEYSQVP IINAGDGSNQ HPTQTLLDLY TIMREIGRID
160 170 180 190 200
GIKIAFVGDL KYGRTVHSLV YALSLFENVE MYFVSPKELR LPKDIIEDLK
210 220 230 240 250
AKNIKFYEKE SLDDLDDDID VLYVTRIQKE RFPDPNEYEK VKGSYKIKRE
260 270 280 290 300
YVEGKKFIIM HPLPRVDEID YDVDDLPQAK YFKQSFYGIP VRMAILKKLI

EDNEGE
Length:306
Mass (Da):35,160
Last modified:November 1, 1996 - v1
Checksum:iCBDC31FC450CEF6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99601.1.
PIRiD64497.

Genome annotation databases

EnsemblBacteriaiAAB99601; AAB99601; MJ_1581.
KEGGimja:MJ_1581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99601.1.
PIRiD64497.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RGWX-ray2.80A/B/C/D/E/F1-306[»]
3E2PX-ray3.00A/B/C/D/E/F/I/J/K/L/M/N1-306[»]
4EKNX-ray2.50B1-306[»]
ProteinModelPortaliQ58976.
SMRiQ58976. Positions 2-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99601; AAB99601; MJ_1581.
KEGGimja:MJ_1581.

Phylogenomic databases

eggNOGiarCOG00911. Archaea.
COG0540. LUCA.
InParanoidiQ58976.
KOiK00609.
OMAiKQSFYGV.
PhylomeDBiQ58976.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BRENDAi2.1.3.2. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58976.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRB_METJA
AccessioniPrimary (citable) accession number: Q58976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 17, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.