Q58970 (GUAAA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	MJ1575

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140221
Regions
	Domain	1 – 188	188	Glutamine amidotransferase type-1
Sites
	Active site	76	1	Nucleophile By similarity
	Active site	163	1	By similarity
	Active site	165	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58970 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3F1DDB0FA1F2D850	FASTA	188	21,020
	10 20 30 40 50 60 MIVILDNGGQ YVHRIHRSLK YIGVSSKIVP NTTPLEEIES NKEVKGIILS GGPDIEKAKN 70 80 90 100 110 120 CIDIALNAKL PILGICLGHQ LIALAYGGEV GRAEAEEYAL TKVYVDKEND LFKNVPREFN 130 140 150 160 170 180 AWASHKDEVK KVPEGFEILA HSDICQVEAM KHKTKPIYGV QFHPEVAHTE YGNEILKNFC KVCGYKFE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99597.1.
PIR	F64496.
RefSeq	NP_248584.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58970.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452484.
GenomeReviews	Gene locus MJ1575 in contig L77117_GR.
KEGG	mja:MJ_1575.
NMPDR	fig|243232.1.peg.1621.
TIGR	MJ1575.
Phylogenomic databases
HOGENOM	HBG292341.
OMA	GQYVHRI.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1575-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METJA

Accession

Primary (citable) accession number: Q58970

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program