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Protein

tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase

Gene

taw2

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 4-demethylwyosine(37) in tRNA(Phe) = S-methyl-5'-thioadenosine + 7-((3S)-3-amino-3-carboxypropyl)-4-demethylwyosine(37) in tRNA(Phe).UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801S-adenosyl-L-methionineUniRule annotation1 Publication
Binding sitei87 – 871S-adenosyl-L-methionineUniRule annotation
Binding sitei127 – 1271S-adenosyl-L-methionineUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.5.1.114. 3260.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferaseUniRule annotation (EC:2.5.1.114UniRule annotation)
Alternative name(s):
MjTYW2
tRNA wyosine derivatives biosynthesis protein Taw2UniRule annotation
Gene namesi
Name:taw2UniRule annotation
Ordered Locus Names:MJ1557
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferasePRO_0000107411Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_1557.

Structurei

Secondary structure

1
249
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Beta strandi11 – 144Combined sources
Beta strandi34 – 385Combined sources
Beta strandi52 – 554Combined sources
Beta strandi60 – 656Combined sources
Beta strandi68 – 736Combined sources
Turni74 – 763Combined sources
Helixi81 – 833Combined sources
Helixi84 – 918Combined sources
Beta strandi99 – 1024Combined sources
Turni106 – 1105Combined sources
Helixi111 – 1177Combined sources
Beta strandi121 – 1277Combined sources
Helixi130 – 14213Combined sources
Beta strandi146 – 1538Combined sources
Helixi155 – 1573Combined sources
Beta strandi164 – 1696Combined sources
Helixi175 – 1784Combined sources
Helixi179 – 1857Combined sources
Beta strandi186 – 19914Combined sources
Helixi200 – 2023Combined sources
Turni203 – 2053Combined sources
Helixi206 – 21712Combined sources
Beta strandi220 – 23415Combined sources
Beta strandi237 – 24812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A27X-ray2.00A1-249[»]
ProteinModelPortaliQ58952.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58952.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 1552S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG10124. Archaea.
COG2520. LUCA.
InParanoidiQ58952.
KOiK07055.
OMAiKYQKIGD.
PhylomeDBiQ58952.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01922. TYW2_archaea. 1 hit.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR030867. TYW2_archaea.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIKYQKIGD VVIVKKELSE DEIREIVKRT KCKAILLYTT QITGEFRTPH
60 70 80 90 100
VKILYGKETE TIHKEYGCLF KLDVAKIMWS QGNIEERKRM AFISNENEVV
110 120 130 140 150
VDMFAGIGYF TIPLAKYSKP KLVYAIEKNP TAYHYLCENI KLNKLNNVIP
160 170 180 190 200
ILADNRDVEL KDVADRVIMG YVHKTHKFLD KTFEFLKDRG VIHYHETVAE
210 220 230 240
KIMYERPIER LKFYAEKNGY KLIDYEVRKI KKYAPGVWHV VVDAKFERI
Length:249
Mass (Da):29,302
Last modified:November 1, 1996 - v1
Checksum:iA4D1EB10B63CA51C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99577.1.
PIRiD64494.

Genome annotation databases

EnsemblBacteriaiAAB99577; AAB99577; MJ_1557.
KEGGimja:MJ_1557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99577.1.
PIRiD64494.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A27X-ray2.00A1-249[»]
ProteinModelPortaliQ58952.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99577; AAB99577; MJ_1557.
KEGGimja:MJ_1557.

Phylogenomic databases

eggNOGiarCOG10124. Archaea.
COG2520. LUCA.
InParanoidiQ58952.
KOiK07055.
OMAiKYQKIGD.
PhylomeDBiQ58952.

Enzyme and pathway databases

BRENDAi2.5.1.114. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58952.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01922. TYW2_archaea. 1 hit.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR030867. TYW2_archaea.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYW2_METJA
AccessioniPrimary (citable) accession number: Q58952
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.