Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-seryl-tRNA(Sec) kinase

Gene

pstK

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.1 Publication

Catalytic activityi

ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-seryl-tRNA(Sec) kinase (pstK)
  2. O-phosphoseryl-tRNA(Sec) selenium transferase (spcS)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 14ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14956.
BRENDAi2.7.1.164. 3260.
UniPathwayiUPA00906; UER00897.

Names & Taxonomyi

Protein namesi
Recommended name:
L-seryl-tRNA(Sec) kinase (EC:2.7.1.164)
Alternative name(s):
O-phosphoseryl-tRNA(Sec) kinase
Short name:
PSTK
Gene namesi
Name:pstK
Ordered Locus Names:MJ1538
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001073961 – 248L-seryl-tRNA(Sec) kinaseAdd BLAST248

Interactioni

Protein-protein interaction databases

DIPiDIP-48971N.
STRINGi243232.MJ_1538.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 6Combined sources6
Helixi13 – 26Combined sources14
Beta strandi31 – 34Combined sources4
Helixi37 – 40Combined sources4
Beta strandi43 – 45Combined sources3
Helixi48 – 50Combined sources3
Helixi51 – 66Combined sources16
Beta strandi69 – 73Combined sources5
Helixi80 – 92Combined sources13
Beta strandi96 – 103Combined sources8
Helixi106 – 115Combined sources10
Helixi122 – 131Combined sources10
Helixi140 – 142Combined sources3
Beta strandi145 – 149Combined sources5
Helixi156 – 167Combined sources12
Beta strandi172 – 174Combined sources3
Helixi186 – 205Combined sources20
Helixi209 – 227Combined sources19
Beta strandi229 – 231Combined sources3
Helixi234 – 247Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A4LX-ray1.80A/B1-248[»]
3A4MX-ray1.79A/B1-248[»]
3A4NX-ray2.50A/B1-248[»]
3ADBX-ray2.80A/B1-248[»]
3ADCX-ray2.90A/B1-248[»]
3ADDX-ray2.40A/B1-248[»]
3AM1X-ray2.40A1-248[»]
ProteinModelPortaliQ58933.
SMRiQ58933.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58933.

Family & Domainsi

Sequence similaritiesi

Belongs to the L-seryl-tRNA(Sec) kinase family.Curated

Phylogenomic databases

eggNOGiarCOG01041. Archaea.
COG4088. LUCA.
InParanoidiQ58933.
KOiK10837.
OMAiYNSKRRD.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. KTI12/PSTK.
IPR020024. L-seryl-tRNA_Sec_kinase_arc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03574. selen_PSTK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIILTGLPG VGKSTFSKNL AKILSKNNID VIVLGSDLIR ESFPVWKEKY
60 70 80 90 100
EEFIKKSTYR LIDSALKNYW VIVDDTNYYN SMRRDLINIA KKYNKNYAII
110 120 130 140 150
YLKASLDVLI RRNIERGEKI PNEVIKKMYE KFDEPGKKYK WDEPFLIIDT
160 170 180 190 200
TKDIDFNEIA KKLIEKSKEI PKFYVLEENK NKNNNISDKI DKETRKIVSE
210 220 230 240
YIKSKKLDKD KIKEVVELRK EFLKKIKKME EVDADRVLKE FKDLLNSY
Length:248
Mass (Da):29,467
Last modified:May 1, 2007 - v2
Checksum:i815EF9A453BEA450
GO

Sequence cautioni

The sequence AAB99557 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99557.1. Different initiation.
PIRiA64492.
RefSeqiWP_064496861.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99557; AAB99557; MJ_1538.
GeneIDi1452446.
KEGGimja:MJ_1538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99557.1. Different initiation.
PIRiA64492.
RefSeqiWP_064496861.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A4LX-ray1.80A/B1-248[»]
3A4MX-ray1.79A/B1-248[»]
3A4NX-ray2.50A/B1-248[»]
3ADBX-ray2.80A/B1-248[»]
3ADCX-ray2.90A/B1-248[»]
3ADDX-ray2.40A/B1-248[»]
3AM1X-ray2.40A1-248[»]
ProteinModelPortaliQ58933.
SMRiQ58933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48971N.
STRINGi243232.MJ_1538.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99557; AAB99557; MJ_1538.
GeneIDi1452446.
KEGGimja:MJ_1538.

Phylogenomic databases

eggNOGiarCOG01041. Archaea.
COG4088. LUCA.
InParanoidiQ58933.
KOiK10837.
OMAiYNSKRRD.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00897.
BioCyciMetaCyc:MONOMER-14956.
BRENDAi2.7.1.164. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58933.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. KTI12/PSTK.
IPR020024. L-seryl-tRNA_Sec_kinase_arc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03574. selen_PSTK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSTK_METJA
AccessioniPrimary (citable) accession number: Q58933
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.