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Protein

L-seryl-tRNA(Sec) kinase

Gene

pstK

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.1 Publication

Catalytic activityi

ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-seryl-tRNA(Sec) kinase (pstK)
  2. O-phosphoseryl-tRNA(Sec) selenium transferase (spcS)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 148ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14956.
BRENDAi2.7.1.164. 3260.
UniPathwayiUPA00906; UER00897.

Names & Taxonomyi

Protein namesi
Recommended name:
L-seryl-tRNA(Sec) kinase (EC:2.7.1.164)
Alternative name(s):
O-phosphoseryl-tRNA(Sec) kinase
Short name:
PSTK
Gene namesi
Name:pstK
Ordered Locus Names:MJ1538
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248L-seryl-tRNA(Sec) kinasePRO_0000107396Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-48971N.
STRINGi243232.MJ_1538.

Structurei

Secondary structure

248
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 66Combined sources
Helixi13 – 2614Combined sources
Beta strandi31 – 344Combined sources
Helixi37 – 404Combined sources
Beta strandi43 – 453Combined sources
Helixi48 – 503Combined sources
Helixi51 – 6616Combined sources
Beta strandi69 – 735Combined sources
Helixi80 – 9213Combined sources
Beta strandi96 – 1038Combined sources
Helixi106 – 11510Combined sources
Helixi122 – 13110Combined sources
Helixi140 – 1423Combined sources
Beta strandi145 – 1495Combined sources
Helixi156 – 16712Combined sources
Beta strandi172 – 1743Combined sources
Helixi186 – 20520Combined sources
Helixi209 – 22719Combined sources
Beta strandi229 – 2313Combined sources
Helixi234 – 24714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A4LX-ray1.80A/B1-248[»]
3A4MX-ray1.79A/B1-248[»]
3A4NX-ray2.50A/B1-248[»]
3ADBX-ray2.80A/B1-248[»]
3ADCX-ray2.90A/B1-248[»]
3ADDX-ray2.40A/B1-248[»]
3AM1X-ray2.40A1-248[»]
ProteinModelPortaliQ58933.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58933.

Family & Domainsi

Sequence similaritiesi

Belongs to the L-seryl-tRNA(Sec) kinase family.Curated

Phylogenomic databases

eggNOGiarCOG01041. Archaea.
COG4088. LUCA.
InParanoidiQ58933.
KOiK10837.
OMAiYNSKRRD.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. KTI12/PSTK.
IPR020024. L-seryl-tRNA_Sec_kinase_arc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03574. selen_PSTK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIILTGLPG VGKSTFSKNL AKILSKNNID VIVLGSDLIR ESFPVWKEKY
60 70 80 90 100
EEFIKKSTYR LIDSALKNYW VIVDDTNYYN SMRRDLINIA KKYNKNYAII
110 120 130 140 150
YLKASLDVLI RRNIERGEKI PNEVIKKMYE KFDEPGKKYK WDEPFLIIDT
160 170 180 190 200
TKDIDFNEIA KKLIEKSKEI PKFYVLEENK NKNNNISDKI DKETRKIVSE
210 220 230 240
YIKSKKLDKD KIKEVVELRK EFLKKIKKME EVDADRVLKE FKDLLNSY
Length:248
Mass (Da):29,467
Last modified:May 1, 2007 - v2
Checksum:i815EF9A453BEA450
GO

Sequence cautioni

The sequence AAB99557.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99557.1. Different initiation.
PIRiA64492.

Genome annotation databases

EnsemblBacteriaiAAB99557; AAB99557; MJ_1538.
KEGGimja:MJ_1538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99557.1. Different initiation.
PIRiA64492.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A4LX-ray1.80A/B1-248[»]
3A4MX-ray1.79A/B1-248[»]
3A4NX-ray2.50A/B1-248[»]
3ADBX-ray2.80A/B1-248[»]
3ADCX-ray2.90A/B1-248[»]
3ADDX-ray2.40A/B1-248[»]
3AM1X-ray2.40A1-248[»]
ProteinModelPortaliQ58933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48971N.
STRINGi243232.MJ_1538.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99557; AAB99557; MJ_1538.
KEGGimja:MJ_1538.

Phylogenomic databases

eggNOGiarCOG01041. Archaea.
COG4088. LUCA.
InParanoidiQ58933.
KOiK10837.
OMAiYNSKRRD.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00897.
BioCyciMetaCyc:MONOMER-14956.
BRENDAi2.7.1.164. 3260.

Miscellaneous databases

EvolutionaryTraceiQ58933.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. KTI12/PSTK.
IPR020024. L-seryl-tRNA_Sec_kinase_arc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03574. selen_PSTK. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Structural and functional investigation of a putative archaeal selenocysteine synthase."
    Kaiser J.T., Gromadski K., Rother M., Engelhardt H., Rodnina M.V., Wahl M.C.
    Biochemistry 44:13315-13327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiPSTK_METJA
AccessioniPrimary (citable) accession number: Q58933
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: May 1, 2007
Last modified: November 11, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.