Reviewed, UniProtKB/Swiss-Prot Q58931 (TRXB_METJA)

Names and origin

Protein names

Recommended name:
    Putative thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Ordered Locus Names:

MJ1536

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond By similarity.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Putative thioredoxin reductase		PRO_0000166759
Regions
	Nucleotide binding	32 – 39	8	FAD By similarity
	Nucleotide binding	272 – 280	9	FAD By similarity
Amino acid modifications
	Disulfide bond	130 ↔ 133		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58931-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B8319F2B33559AD8	FASTA	301	33,035
	10 20 30 40 50 60 MIHDTIIIGA GPGGLTAGIY AMRGKLNALC IEKENAGGRI AEAGIVENYP GFEEIRGYEL 70 80 90 100 110 120 AEKFKNHAEK FKLPIIYDEV IKIETKERPF KVITKNSEYL TKTIVIATGT KPKKLGLNED 130 140 150 160 170 180 KFIGRGISYC TMCDAFFYLN KEVIVIGRDT PAIMSAINLK DIAKKVIVIT DKSELKAAES 190 200 210 220 230 240 IMLDKLKEAN NVEIIYNAKP LEIVGEERAE GVKISVNGKE EIIKADGIFI SLGHVPNTEF 250 260 270 280 290 300 LKDSGIELDK KGFIKTDENC RTNIDGIYAV GDVRGGVMQV AKAVGDGCVA MANIIKYLQK L

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99556.1.
PIR	G64491.
RefSeq	NP_248544.1.
3D structure databases
HSSP	HSSP built from PDB template 1CL0 based on UniProtKB P09625.
ModBase	Search...
Genome annotation databases
GeneID	1452444.
GenomeReviews	Gene locus MJ1536 in contig L77117_GR.
KEGG	mja:MJ1536.
NMPDR	fig|243232.1.peg.1581.
TIGR	MJ1536.
Phylogenomic databases
HOGENOM	Q58931.
OMA	Q58931. IIYNAKP.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1536-MON.
BRENDA	1.8.1.9. 256362.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_METJA

Accession

Primary (citable) accession number: Q58931

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)