Q58929 (MER_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	MJ1534

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subcellular location	Cytoplasm By similarity HAMAP MF_01091.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_0000084807

Sequences

	Sequence		Length	Mass (Da)
	Q58929 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: 367B5DFD1EB6EBBD	FASTA	331	35,353
	10 20 30 40 50 60 MKFGIEFVPN EPIQKLCYYV KLAEDNGFEY CWITDHYNNR NVYMALTAIA MNTNKIKLGP 70 80 90 100 110 120 GVTNPYVRSP AITASAIATL DELSGGRAVL GIGPGDKATF DALGIEWVKP VTTLKESIEV 130 140 150 160 170 180 IRKLLAGERV SYEGKVVKIA GAALAVKPIQ KAVPVYMGAQ GPKMLETAGM IADGVLINAS 190 200 210 220 230 240 NPKDFEAAIP LIKKGAEAAG RSMDEIDVAA YACMSVDKNA DKAKQAAVPV VAFIAAGSPP 250 260 270 280 290 300 VVLERHGIDM EKVEAIRNAL KSGNFPEAFK NVDDTMLEAF SIYGTPEDVV EKCKKLAEMG 310 320 330 VTQIVAGSPI GPNKETAIKL IGKKVIPALK E

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99555.1.
RefSeq	NP_248542.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58929.
SMR	Q58929. Positions 1-325.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1452442.
GenomeReviews	Gene locus MJ1534 in contig L77117_GR.
KEGG	mja:MJ_1534.
NMPDR	fig|243232.1.peg.1579.
TIGR	MJ1534.
Phylogenomic databases
HOGENOM	HBG497169.
OMA	MGVTQIV.
ProtClustDB	PRK02271.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1534-MONOMER.
Family and domain databases
HAMAP	MF_01091. F420_mer. [Tree]
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
KO	K00320.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_METJA

Accession

Primary (citable) accession number: Q58929

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 1998
Last modified:	November 16, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program