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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation1 Publication

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei128 – 1281Nucleophile; methyl group acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:MJ1529
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Methylated-DNA--protein-cysteine methyltransferasePRO_0000139377Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_1529.

Structurei

Secondary structure

1
167
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Beta strandi8 – 2316Combined sources
Helixi31 – 344Combined sources
Turni44 – 474Combined sources
Helixi48 – 5811Combined sources
Turni67 – 704Combined sources
Helixi82 – 887Combined sources
Helixi99 – 1057Combined sources
Helixi110 – 1189Combined sources
Turni123 – 1253Combined sources
Helixi127 – 1304Combined sources
Beta strandi134 – 1363Combined sources
Beta strandi140 – 1423Combined sources
Helixi145 – 15511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G7HNMR-A1-167[»]
ProteinModelPortaliQ58924.
SMRiQ58924. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58924.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
InParanoidiQ58924.
KOiK00567.
OMAiILKLYFA.
PhylomeDBiQ58924.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIQIEEYFI GMIFKGNQLV RNTIPLRREE IFNFMDGEVV SNPEDEHLKV
60 70 80 90 100
AEIILKLYFA EIDDKKVREL ISYKLEVPEF TKKVLDIVKD IEFGKTLTYG
110 120 130 140 150
DIAKKLNTSP RAVGMALKRN PLPLIIPCHR VVAKNSLGGY SYGLDKKKFI
160
LERERLNMVS FKFNKVY
Length:167
Mass (Da):19,431
Last modified:November 1, 1996 - v1
Checksum:i7FC44483D31F6EF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99547.1.
PIRiH64490.

Genome annotation databases

EnsemblBacteriaiAAB99547; AAB99547; MJ_1529.
KEGGimja:MJ_1529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99547.1.
PIRiH64490.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G7HNMR-A1-167[»]
ProteinModelPortaliQ58924.
SMRiQ58924. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99547; AAB99547; MJ_1529.
KEGGimja:MJ_1529.

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
InParanoidiQ58924.
KOiK00567.
OMAiILKLYFA.
PhylomeDBiQ58924.

Miscellaneous databases

EvolutionaryTraceiQ58924.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Structural studies of MJ1529, an O6-methylguanine-DNA methyltransferase."
    Roberts A., Pelton J.G., Wemmer D.E.
    Magn. Reson. Chem. 44:S71-S82(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiOGT_METJA
AccessioniPrimary (citable) accession number: Q58924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: February 17, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.