Reviewed, UniProtKB/Swiss-Prot Q58893 (MTM5_METJA)

Names and origin

Protein names

Recommended name:
    Modification methylase MjaV
      Short name=M.MjaV
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase MjaV

Gene names

Name:	mjaVM
Ordered Locus Names:	MJ1498

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTAC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaV endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Modification methylase MjaV		PRO_0000087938

Sequences

	Sequence		Length	Mass (Da)
	Q58893-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A5A46D7C8FBD3FE3	FASTA	292	33,877
	10 20 30 40 50 60 MEINKIYCMD CLEGMKQLKD KTVDVVVTSP PYNIGIKYNK YSDNLSREDY LNWIEEVVKE 70 80 90 100 110 120 IKRVLKDDGS FFINVGYTAK DPWIAFDVAN VIRKHFKLQN TIHWVKSIAI QKEDVGNYPN 130 140 150 160 170 180 IIGDIAVGHY KPINSDRFLS IMHEYIFHFT KNGNVKLDKL AIGVPYQDKS NIKRFNRKGD 190 200 210 220 230 240 LRDRGNTWFI PYETIQSKEK ERPHPATFPP KLPEMCIKLH GVKKTNLVLD PFMGIGSTAI 250 260 270 280 290 ACIRLGIDYI GFEIDEYYCR VAEERIKKEL LKTDGKFDNV KNKNIITLDA FI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99509.1.
PIR	A64487.
RefSeq	NP_248505.1.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Genome annotation databases
GeneID	1452405.
GenomeReviews	Gene locus MJ1498 in contig L77117_GR.
KEGG	mja:MJ1498.
NMPDR	fig|243232.1.peg.1542.
TIGR	MJ1498.
Phylogenomic databases
HOGENOM	Q58893.
OMA	NIGFFAR.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1498-MON.
BRENDA	2.1.1.113. 256362.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM5_METJA

Accession

Primary (citable) accession number: Q58893

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)