Q58893 (MTM5_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase MjaV
Short name=M.MjaV
EC=2.1.1.113

Alternative name(s):
N-4 cytosine-specific methyltransferase MjaV

Gene names

Name:	mjaVM
Ordered Locus Names:	MJ1498

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTAC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaV endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Modification methylase MjaV		PRO_0000087938

Sequences

	Sequence		Length	Mass (Da)
	Q58893 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A5A46D7C8FBD3FE3	FASTA	292	33,877
	10 20 30 40 50 60 MEINKIYCMD CLEGMKQLKD KTVDVVVTSP PYNIGIKYNK YSDNLSREDY LNWIEEVVKE 70 80 90 100 110 120 IKRVLKDDGS FFINVGYTAK DPWIAFDVAN VIRKHFKLQN TIHWVKSIAI QKEDVGNYPN 130 140 150 160 170 180 IIGDIAVGHY KPINSDRFLS IMHEYIFHFT KNGNVKLDKL AIGVPYQDKS NIKRFNRKGD 190 200 210 220 230 240 LRDRGNTWFI PYETIQSKEK ERPHPATFPP KLPEMCIKLH GVKKTNLVLD PFMGIGSTAI 250 260 270 280 290 ACIRLGIDYI GFEIDEYYCR VAEERIKKEL LKTDGKFDNV KNKNIITLDA FI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99509.1.
PIR	A64487.
RefSeq	NP_248505.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58893.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ1498.
Protein family/group databases
REBASE	3897. M.MjaV.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB99509; AAB99509; MJ_1498.
GeneID	1452405.
KEGG	mja:MJ_1498.
Phylogenomic databases
eggNOG	COG0863.
KO	K00571. K00590.
OMA	DTWFIPY.
ProtClustDB	CLSK876599.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR017985. MeTrfase_CN4_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM5_METJA

Accession

Primary (citable) accession number: Q58893

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program