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Q58844

- T2M2_METJA

UniProt

Q58844 - T2M2_METJA

Protein

Type-2 restriction enzyme MjaII

Gene

mjaIIR

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (03 Sep 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGNCC.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1222. MjaII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MjaII (EC:3.1.21.4)
    Short name:
    R.MjaII
    Alternative name(s):
    Endonuclease MjaII
    Type II restriction enzyme MjaII
    Gene namesi
    Name:mjaIIR
    Ordered Locus Names:MJ1449
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Type-2 restriction enzyme MjaIIPRO_0000077333Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243232.MJ1449.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG136805.
    OMAiPNAGEMR.

    Family and domain databases

    InterProiIPR019045. Restrct_endonuc_II_TdeIII.
    [Graphical view]
    PfamiPF09520. RE_TdeIII. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q58844-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLSKNVIEK ISIETIRVLK SRFDTISDED IKIRNMPFHM AFLRAFYGKI    50
    GINDDTEALK FLTLSQWFHG LSTTLGQSYF ENIAHILSNG EKRTFKNYKI 100
    KRSVRDKISE IINDLKSGER LPNVEKENKE LREATSKNSE YVNGLEFTAD 150
    VYFEDKDKVV MIELKTVRPN AGEMRGEKQK ILYGKAYMME TKPNKKVYYF 200
    IGFPYDPTEN PENPCGYDKD RFMSSLIEFS KYFDKREVLI AEELWSFLSG 250
    EENTMKKILD IINSIAKPDF KEKFDFINTF PFINQDRLYT KDAIDEQKFK 300
    KYMDILQEWR LYSEIECAKA VKELSLLKLS SRDRRTFERL INNSMFSNNN 350
    KYNENRKMKI LELYNKYMQK 370
    Length:370
    Mass (Da):43,911
    Last modified:November 1, 1996 - v1
    Checksum:i8B4CF6208F914B33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99461.1.
    PIRiH64480.
    RefSeqiNP_248453.1. NC_000909.1.
    WP_010870969.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99461; AAB99461; MJ_1449.
    GeneIDi1452353.
    KEGGimja:MJ_1449.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99461.1 .
    PIRi H64480.
    RefSeqi NP_248453.1. NC_000909.1.
    WP_010870969.1. NC_000909.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1449.

    Protein family/group databases

    REBASEi 1222. MjaII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99461 ; AAB99461 ; MJ_1449 .
    GeneIDi 1452353.
    KEGGi mja:MJ_1449.

    Phylogenomic databases

    eggNOGi NOG136805.
    OMAi PNAGEMR.

    Family and domain databases

    InterProi IPR019045. Restrct_endonuc_II_TdeIII.
    [Graphical view ]
    Pfami PF09520. RE_TdeIII. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiT2M2_METJA
    AccessioniPrimary (citable) accession number: Q58844
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: September 3, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3