ID   MTM2_METJA              Reviewed;         530 AA.
AC   Q58843;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 121.
DE   RecName: Full=Type II methyltransferase M.MjaII {ECO:0000303|PubMed:12654995};
DE            Short=M.MjaII {ECO:0000303|PubMed:12654995};
DE            EC=2.1.1.113;
DE   AltName: Full=N-4 cytosine-specific methyltransferase MjaII;
GN   Name=mjaIIM; OrderedLocusNames=MJ1448;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   CHARACTERIZATION.
RA   Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D.;
RT   "Method for screening restriction endonucleases.";
RL   Patent number WO9911821, 11-MAR-1999.
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: An alpha subtype methylase that recognizes the double-
CC       stranded sequence 5'-GGNCC-3', methylates C-5 on both strands, and
CC       protects the DNA from cleavage by the MjaII endonuclease.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|Ref.2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC         methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; L77117; AAB99457.1; -; Genomic_DNA.
DR   PIR; G64480; G64480.
DR   RefSeq; WP_010870968.1; NC_000909.1.
DR   AlphaFoldDB; Q58843; -.
DR   STRING; 243232.MJ_1448; -.
DR   REBASE; 3896; M.MjaII.
DR   PaxDb; 243232-MJ_1448; -.
DR   EnsemblBacteria; AAB99457; AAB99457; MJ_1448.
DR   GeneID; 1452352; -.
DR   KEGG; mja:MJ_1448; -.
DR   eggNOG; arCOG00890; Archaea.
DR   HOGENOM; CLU_039482_0_0_2; -.
DR   InParanoid; Q58843; -.
DR   OrthoDB; 38200at2157; -.
DR   PhylomeDB; Q58843; -.
DR   PRO; PR:Q58843; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR017985; MeTrfase_CN4_CS.
DR   InterPro; IPR001091; RM_Methyltransferase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR   PANTHER; PTHR13370:SF33; TYPE II METHYLTRANSFERASE M.MJAV; 1.
DR   Pfam; PF01555; N6_N4_Mtase; 2.
DR   PRINTS; PR00508; S21N4MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR   PROSITE; PS00093; N4_MTASE; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Methyltransferase; Reference proteome; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..530
FT                   /note="Type II methyltransferase M.MjaII"
FT                   /id="PRO_0000087937"
SQ   SEQUENCE   530 AA;  63755 MW;  FF6F29ADD080060C CRC64;
     MNLDAWLDIQ PAKKLYTIKE ASRILTKKFG KEIKEHNISY LVQYGRVNKY KIKNRVYVDI
     DEVENYYKKL FFEKRKEWEE KLGFKLDWDL AFDLLSEKER TKHVHGIHPY KGKFIPQLVE
     YFLKRHFNVG DIIIDPFMGS GTTLVQCMEM GINSIGIDIS PFNCLIAEVK LQKYDIQKLK
     KILLDMLNKT KEFSKNLGDD EFVKEMDKLI EKYNKKYFTL EYKRKLSKKE IDEDSYSEKI
     MEMFYLEYKK LKEKYCKNDD EFDDIFKDKP FLYKWYSPRI RAELNFYLNL IKDCRDETIK
     KVAMIILSRT ARSVRGTTHF DLATLKEPVF DPYYCYKHKK ICRPVQTILR HLEEYTNDVI
     SRIEEFSKIR KDAYYLIING DSRTVDIEEE LKKHPNFYEL YKNKKIDGIF TSPPYLGQID
     YHEQHAYAYE LFDIPRLDEL EIGPKFKGSS KKAQKEYIEG ISDVLINMKR FLNEDAKIFI
     VVNDKKNLYK EIFEKSGLIL VREFKRPVLN RTERDRNPYY ESIFELKMEE
//