Reviewed, UniProtKB/Swiss-Prot Q58843 (MTM2_METJA)

Names and origin

Protein names

Recommended name:
    Modification methylase MjaII
      Short name=M.MjaII
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase MjaII

Gene names

Name:	mjaIIM
Ordered Locus Names:	MJ1448

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGNC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaII endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Modification methylase MjaII		PRO_0000087937

Sequences

	Sequence		Length	Mass (Da)
	Q58843-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FF6F29ADD080060C	FASTA	530	63,755
	10 20 30 40 50 60 MNLDAWLDIQ PAKKLYTIKE ASRILTKKFG KEIKEHNISY LVQYGRVNKY KIKNRVYVDI 70 80 90 100 110 120 DEVENYYKKL FFEKRKEWEE KLGFKLDWDL AFDLLSEKER TKHVHGIHPY KGKFIPQLVE 130 140 150 160 170 180 YFLKRHFNVG DIIIDPFMGS GTTLVQCMEM GINSIGIDIS PFNCLIAEVK LQKYDIQKLK 190 200 210 220 230 240 KILLDMLNKT KEFSKNLGDD EFVKEMDKLI EKYNKKYFTL EYKRKLSKKE IDEDSYSEKI 250 260 270 280 290 300 MEMFYLEYKK LKEKYCKNDD EFDDIFKDKP FLYKWYSPRI RAELNFYLNL IKDCRDETIK 310 320 330 340 350 360 KVAMIILSRT ARSVRGTTHF DLATLKEPVF DPYYCYKHKK ICRPVQTILR HLEEYTNDVI 370 380 390 400 410 420 SRIEEFSKIR KDAYYLIING DSRTVDIEEE LKKHPNFYEL YKNKKIDGIF TSPPYLGQID 430 440 450 460 470 480 YHEQHAYAYE LFDIPRLDEL EIGPKFKGSS KKAQKEYIEG ISDVLINMKR FLNEDAKIFI 490 500 510 520 530 VVNDKKNLYK EIFEKSGLIL VREFKRPVLN RTERDRNPYY ESIFELKMEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB99457.1.
PIR	G64480.
RefSeq	NP_248452.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1452352.
GenomeReviews	Gene locus MJ1448 in contig L77117_GR.
KEGG	mja:MJ1448.
NMPDR	fig|243232.1.peg.1489.
TIGR	MJ1448.
Phylogenomic databases
HOGENOM	Q58843.
OMA	IDYHEQH.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_1448-MON.
BRENDA	2.1.1.113. 256362.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM2_METJA

Accession

Primary (citable) accession number: Q58843

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)