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Protein

Replication factor C small subunit

Gene

rfcS

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
Cleaved into the following 3 chains:
Gene namesi
Name:rfcS
Ordered Locus Names:MJ1422
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000303611 – 53Replication factor C small subunit, 1st partSequence analysisAdd BLAST53
ChainiPRO_000003036254 – 601Mja RFC-1 inteinSequence analysisAdd BLAST548
ChainiPRO_0000030363602 – 626Replication factor C small subunit, 2nd partSequence analysisAdd BLAST25
ChainiPRO_0000030364627 – 1062Mja RFC-2 inteinSequence analysisAdd BLAST436
ChainiPRO_00000303651063 – 1124Replication factor C small subunit, 3rd partSequence analysisAdd BLAST62
ChainiPRO_00000303661125 – 1667Mja RFC-3 inteinSequence analysisAdd BLAST543
ChainiPRO_00000303671668 – 1847Replication factor C small subunit, 4th partSequence analysisAdd BLAST180

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ58817.

Interactioni

Subunit structurei

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).By similarity

Protein-protein interaction databases

STRINGi243232.MJ_1422.

Structurei

3D structure databases

ProteinModelPortaliQ58817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 311DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd BLAST133
Domaini780 – 927DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd BLAST148
Domaini1348 – 1508DOD-type homing endonuclease 3PROSITE-ProRule annotationAdd BLAST161

Sequence similaritiesi

Contains 3 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG00469. Archaea.
arCOG03145. Archaea.
arCOG03154. Archaea.
arCOG03158. Archaea.
COG0470. LUCA.
InParanoidiQ58817.
KOiK04801.
OMAiTNDENEM.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.170.16.10. 7 hits.
3.10.28.10. 5 hits.
3.40.50.300. 4 hits.
InterProiIPR001387. Cro/C1-type_HTH.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
IPR008824. RuvB_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 3 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 7 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 3 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 3 hits.
PROSITEiPS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 3 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVIIMEKPWV EKYRPKTLDD IVGQDEIVKR LKKYVEKKSM PHLLFSGPPG
60 70 80 90 100
VGKCLTGDTK VIVNGEIREI GEVIEEISNG KFGVTLTNNL KVLGIDEDGK
110 120 130 140 150
IREFDVQYVY KDKTNTLIKI KTKMGRELKV TTYHPLLINH KNGEIKWEKA
160 170 180 190 200
ENLKVGDKLA TPRYILFNES DYNEELAEWL GYFIGDGHAD KESNKITFTN
210 220 230 240 250
GDEKLRKRFA ELTEKLFKDA KIKERIHKDR TPDIYVNSKE AVEFIDKLGL
260 270 280 290 300
RGKKADKVRI PKEIMRSDAL RAFLRAYFDC DGGIEKHSIV LSTASKEMAE
310 320 330 340 350
DLVYALLRFG IIAKLREKVN KNNNKVYYHI VISNSSNLRT FLDNIGFSQE
360 370 380 390 400
RKLKKLLEII KDENPNLDVI TIDKEKIRYI RDRLKVKLTR DIEKDNWSYN
410 420 430 440 450
KCRKITQELL KEIYYRLEEL KEIEKALEEN ILIDWDEVAE RRKEIAEKTG
460 470 480 490 500
IRSDRILEYI RGKRKPSLKN YIKIANTLGK NIEKIIDAMR IFAKKYSSYA
510 520 530 540 550
EIGKMLNMWN SSIKIYLESN TQEIEKLEEI RKTELKLVKE ILNDEKLIDS
560 570 580 590 600
IGYVLFLASN EIYWDEIVEI EQLNGEFTIY DLHVPRYHNF IGGNLPTILH
610 620 630 640 650
NTTAALCLAR DLFGENWRDN FLELNASVSK DTPILVKIDG KVKRTTFEEL
660 670 680 690 700
DKIYFETNDE NEMYKKVDNL EVLTVDENFR VRWRKVSTII RHKVDKILRI
710 720 730 740 750
KFEGGYIELT GNHSIMMLDE NGLVAKKASD IKVGDCFLSF VANIEGEKDR
760 770 780 790 800
LDLKEFEPKD ITSRVKIIND FDIDEDTAWM LGLYVAEGAV GFKGKTSGQV
810 820 830 840 850
IYTLGSHEHD LINKLNDIVD KKGFSKYENF TGSGFDRKRL SAKQIRILNT
860 870 880 890 900
QLARFVEENF YDGNGRRARN KRIPDIIFEL KENLRVEFLK GLADGDSSGN
910 920 930 940 950
WREVVRISSK SDNLLIDTVW LARISGIESS IFENEARLIW KGGMKWKKSN
960 970 980 990 1000
LLPAEPIIKM IKKLENKING NWRYILRHQL YEGKKRVSKD KIKQILEMVN
1010 1020 1030 1040 1050
VEKLSDKEKE VYDLLKKLSK TELYALVVKE IEIIDYNDFV YDVSVPNNEM
1060 1070 1080 1090 1100
FFAGNVPILL HNSDERGIDV IRTKVKDFAR TKPIGDVPFK IIFLDESDAL
1110 1120 1130 1140 1150
TADAQNALRR TMEKYSDVCR FILSCLTGDA KITLPDEREI KIEDFIKMFE
1160 1170 1180 1190 1200
ERKLKHVLNR NGEDLVLAGV KFNSKIVNHK VYRLVLESGR EIEATGDHKF
1210 1220 1230 1240 1250
LTRDGWKEVY ELKEDDEVLV YPALEGVGFE VDERRIIGLN EFYEFLTNYE
1260 1270 1280 1290 1300
IKLGYKPLGK AKSYKELITR DKEKILSRVL ELSDKYSKSE IRRKIEEEFG
1310 1320 1330 1340 1350
IKISLTTIKN LINGKIDGFA LKYVRKIKEL GWDEITYDDE KAGIFARLLG
1360 1370 1380 1390 1400
FIIGDGHLSK SKEGRILITA TINELEGIKK DLEKLGIKAS NIIEKDIEHK
1410 1420 1430 1440 1450
LDGREIKGKT SFIYINNKAF YLLLNFWGVE IGNKTINGYN IPKWIKYGNK
1460 1470 1480 1490 1500
FVKREFLRGL FGADGTKPYI KKYNINGIKL GIRVENISKD KTLEFFEEVK
1510 1520 1530 1540 1550
KMLEEFEVES YIKVSKIDNK NLTELIVKAN NKNYLKYLSR ISYAYEKDNF
1560 1570 1580 1590 1600
ARLVGEYLRI KEAYKDIILK EIAENALKEA DGEKSLRELA RKYNVPVDFI
1610 1620 1630 1640 1650
INQLKGKDIG LPRNFMTFEE FLKEKVVDGK YVSERIIKKE CIGYRDVYDI
1660 1670 1680 1690 1700
TCHKDPSFIA NGFVSHNCNY PSKIIPPIQS RCAVFRFSPL KKEDIAKKLK
1710 1720 1730 1740 1750
EIAEKEGLNL TESGLEAIIY VSEGDMRKAI NVLQTAAALS DVIDDEIVYK
1760 1770 1780 1790 1800
VSSRARPEEV KKMMELALDG KFMEARDLLY KLMVEWGMSG EDILNQMFRE
1810 1820 1830 1840
INSLDIDERK KVELADAIGE TDFRIVEGAN ERIQLSALLA KMALMGR
Length:1,847
Mass (Da):214,097
Last modified:November 1, 1996 - v1
Checksum:i44EADB5B4D6DE64A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99433.1.
PIRiE64477.
RefSeqiWP_010870940.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99433; AAB99433; MJ_1422.
GeneIDi1452326.
KEGGimja:MJ_1422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB99433.1.
PIRiE64477.
RefSeqiWP_010870940.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ58817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_1422.

Proteomic databases

PRIDEiQ58817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB99433; AAB99433; MJ_1422.
GeneIDi1452326.
KEGGimja:MJ_1422.

Phylogenomic databases

eggNOGiarCOG00469. Archaea.
arCOG03145. Archaea.
arCOG03154. Archaea.
arCOG03158. Archaea.
COG0470. LUCA.
InParanoidiQ58817.
KOiK04801.
OMAiTNDENEM.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.170.16.10. 7 hits.
3.10.28.10. 5 hits.
3.40.50.300. 4 hits.
InterProiIPR001387. Cro/C1-type_HTH.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
IPR008824. RuvB_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 3 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 7 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 3 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 3 hits.
PROSITEiPS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 3 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFCS_METJA
AccessioniPrimary (citable) accession number: Q58817
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the potential ATP-binding site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.