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Q58817 (RFCS_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Replication factor C small subunit

Short name=RFC small subunit
Alternative name(s):
Clamp loader small subunit

Cleaved into the following 3 chains:

  1. Mja RFC-1 intein
  2. Mja RFC-2 intein
  3. Mja RFC-3 intein
Gene names
Name:rfcS
Ordered Locus Names:MJ1422
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length1847 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA By similarity.

Subunit structure

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL) By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Miscellaneous

The intein interrupts the potential ATP-binding site.

Sequence similarities

Belongs to the activator 1 small subunits family. RfcS subfamily.

Contains 3 DOD-type homing endonuclease domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5353Replication factor C small subunit, 1st part Potential
PRO_0000030361
Chain54 – 601548Mja RFC-1 intein Potential
PRO_0000030362
Chain602 – 62625Replication factor C small subunit, 2nd part Potential
PRO_0000030363
Chain627 – 1062436Mja RFC-2 intein Potential
PRO_0000030364
Chain1063 – 112462Replication factor C small subunit, 3rd part Potential
PRO_0000030365
Chain1125 – 1667543Mja RFC-3 intein Potential
PRO_0000030366
Chain1668 – 1847180Replication factor C small subunit, 4th part Potential
PRO_0000030367

Regions

Domain179 – 311133DOD-type homing endonuclease 1
Domain780 – 927148DOD-type homing endonuclease 2
Domain1348 – 1508161DOD-type homing endonuclease 3

Sequences

Sequence LengthMass (Da)Tools
Q58817 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 44EADB5B4D6DE64A

FASTA1,847214,097
        10         20         30         40         50         60 
MVIIMEKPWV EKYRPKTLDD IVGQDEIVKR LKKYVEKKSM PHLLFSGPPG VGKCLTGDTK 

        70         80         90        100        110        120 
VIVNGEIREI GEVIEEISNG KFGVTLTNNL KVLGIDEDGK IREFDVQYVY KDKTNTLIKI 

       130        140        150        160        170        180 
KTKMGRELKV TTYHPLLINH KNGEIKWEKA ENLKVGDKLA TPRYILFNES DYNEELAEWL 

       190        200        210        220        230        240 
GYFIGDGHAD KESNKITFTN GDEKLRKRFA ELTEKLFKDA KIKERIHKDR TPDIYVNSKE 

       250        260        270        280        290        300 
AVEFIDKLGL RGKKADKVRI PKEIMRSDAL RAFLRAYFDC DGGIEKHSIV LSTASKEMAE 

       310        320        330        340        350        360 
DLVYALLRFG IIAKLREKVN KNNNKVYYHI VISNSSNLRT FLDNIGFSQE RKLKKLLEII 

       370        380        390        400        410        420 
KDENPNLDVI TIDKEKIRYI RDRLKVKLTR DIEKDNWSYN KCRKITQELL KEIYYRLEEL 

       430        440        450        460        470        480 
KEIEKALEEN ILIDWDEVAE RRKEIAEKTG IRSDRILEYI RGKRKPSLKN YIKIANTLGK 

       490        500        510        520        530        540 
NIEKIIDAMR IFAKKYSSYA EIGKMLNMWN SSIKIYLESN TQEIEKLEEI RKTELKLVKE 

       550        560        570        580        590        600 
ILNDEKLIDS IGYVLFLASN EIYWDEIVEI EQLNGEFTIY DLHVPRYHNF IGGNLPTILH 

       610        620        630        640        650        660 
NTTAALCLAR DLFGENWRDN FLELNASVSK DTPILVKIDG KVKRTTFEEL DKIYFETNDE 

       670        680        690        700        710        720 
NEMYKKVDNL EVLTVDENFR VRWRKVSTII RHKVDKILRI KFEGGYIELT GNHSIMMLDE 

       730        740        750        760        770        780 
NGLVAKKASD IKVGDCFLSF VANIEGEKDR LDLKEFEPKD ITSRVKIIND FDIDEDTAWM 

       790        800        810        820        830        840 
LGLYVAEGAV GFKGKTSGQV IYTLGSHEHD LINKLNDIVD KKGFSKYENF TGSGFDRKRL 

       850        860        870        880        890        900 
SAKQIRILNT QLARFVEENF YDGNGRRARN KRIPDIIFEL KENLRVEFLK GLADGDSSGN 

       910        920        930        940        950        960 
WREVVRISSK SDNLLIDTVW LARISGIESS IFENEARLIW KGGMKWKKSN LLPAEPIIKM 

       970        980        990       1000       1010       1020 
IKKLENKING NWRYILRHQL YEGKKRVSKD KIKQILEMVN VEKLSDKEKE VYDLLKKLSK 

      1030       1040       1050       1060       1070       1080 
TELYALVVKE IEIIDYNDFV YDVSVPNNEM FFAGNVPILL HNSDERGIDV IRTKVKDFAR 

      1090       1100       1110       1120       1130       1140 
TKPIGDVPFK IIFLDESDAL TADAQNALRR TMEKYSDVCR FILSCLTGDA KITLPDEREI 

      1150       1160       1170       1180       1190       1200 
KIEDFIKMFE ERKLKHVLNR NGEDLVLAGV KFNSKIVNHK VYRLVLESGR EIEATGDHKF 

      1210       1220       1230       1240       1250       1260 
LTRDGWKEVY ELKEDDEVLV YPALEGVGFE VDERRIIGLN EFYEFLTNYE IKLGYKPLGK 

      1270       1280       1290       1300       1310       1320 
AKSYKELITR DKEKILSRVL ELSDKYSKSE IRRKIEEEFG IKISLTTIKN LINGKIDGFA 

      1330       1340       1350       1360       1370       1380 
LKYVRKIKEL GWDEITYDDE KAGIFARLLG FIIGDGHLSK SKEGRILITA TINELEGIKK 

      1390       1400       1410       1420       1430       1440 
DLEKLGIKAS NIIEKDIEHK LDGREIKGKT SFIYINNKAF YLLLNFWGVE IGNKTINGYN 

      1450       1460       1470       1480       1490       1500 
IPKWIKYGNK FVKREFLRGL FGADGTKPYI KKYNINGIKL GIRVENISKD KTLEFFEEVK 

      1510       1520       1530       1540       1550       1560 
KMLEEFEVES YIKVSKIDNK NLTELIVKAN NKNYLKYLSR ISYAYEKDNF ARLVGEYLRI 

      1570       1580       1590       1600       1610       1620 
KEAYKDIILK EIAENALKEA DGEKSLRELA RKYNVPVDFI INQLKGKDIG LPRNFMTFEE 

      1630       1640       1650       1660       1670       1680 
FLKEKVVDGK YVSERIIKKE CIGYRDVYDI TCHKDPSFIA NGFVSHNCNY PSKIIPPIQS 

      1690       1700       1710       1720       1730       1740 
RCAVFRFSPL KKEDIAKKLK EIAEKEGLNL TESGLEAIIY VSEGDMRKAI NVLQTAAALS 

      1750       1760       1770       1780       1790       1800 
DVIDDEIVYK VSSRARPEEV KKMMELALDG KFMEARDLLY KLMVEWGMSG EDILNQMFRE 

      1810       1820       1830       1840 
INSLDIDERK KVELADAIGE TDFRIVEGAN ERIQLSALLA KMALMGR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB99433.1.
PIRE64477.
RefSeqNP_248426.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58817.
SMRQ58817. Positions 2-53, 1058-1125, 1667-1847.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ1422.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB99433; AAB99433; MJ_1422.
GeneID1452326.
KEGGmja:MJ_1422.

Phylogenomic databases

eggNOGCOG1372.
KOK04801.
OMATNDENEM.

Family and domain databases

Gene3D1.10.260.40. 1 hit.
2.170.16.10. 7 hits.
3.10.28.10. 5 hits.
3.40.50.300. 4 hits.
InterProIPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]
PfamPF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 3 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 3 hits.
SM00306. HintN. 3 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 7 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 3 hits.
TIGRFAMsTIGR01443. intein_Cterm. 3 hits.
TIGR01445. intein_Nterm. 3 hits.
PROSITEPS50818. INTEIN_C_TER. 3 hits.
PS50819. INTEIN_ENDONUCLEASE. 3 hits.
PS50817. INTEIN_N_TER. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFCS_METJA
AccessionPrimary (citable) accession number: Q58817
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

Intein-containing proteins

List of intein-containing protein entries