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Q58817

- RFCS_METJA

UniProt

Q58817 - RFCS_METJA

Protein

Replication factor C small subunit

Gene

rfcS

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. endonuclease activity Source: InterPro
    3. four-way junction helicase activity Source: InterPro
    4. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: InterPro
    2. DNA repair Source: InterPro
    3. DNA replication Source: UniProtKB-KW
    4. intein-mediated protein splicing Source: InterPro

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication factor C small subunit
    Short name:
    RFC small subunit
    Alternative name(s):
    Clamp loader small subunit
    Cleaved into the following 3 chains:
    Gene namesi
    Name:rfcS
    Ordered Locus Names:MJ1422
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5353Replication factor C small subunit, 1st partSequence AnalysisPRO_0000030361Add
    BLAST
    Chaini54 – 601548Mja RFC-1 inteinSequence AnalysisPRO_0000030362Add
    BLAST
    Chaini602 – 62625Replication factor C small subunit, 2nd partSequence AnalysisPRO_0000030363Add
    BLAST
    Chaini627 – 1062436Mja RFC-2 inteinSequence AnalysisPRO_0000030364Add
    BLAST
    Chaini1063 – 112462Replication factor C small subunit, 3rd partSequence AnalysisPRO_0000030365Add
    BLAST
    Chaini1125 – 1667543Mja RFC-3 inteinSequence AnalysisPRO_0000030366Add
    BLAST
    Chaini1668 – 1847180Replication factor C small subunit, 4th partSequence AnalysisPRO_0000030367Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).By similarity

    Protein-protein interaction databases

    STRINGi243232.MJ1422.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58817.
    SMRiQ58817. Positions 2-53, 1058-1125, 1667-1847.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini179 – 311133DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini780 – 927148DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1348 – 1508161DOD-type homing endonuclease 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1372.
    KOiK04801.
    OMAiTNDENEM.

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    2.170.16.10. 7 hits.
    3.10.28.10. 5 hits.
    3.40.50.300. 4 hits.
    InterProiIPR001387. Cro/C1-type_HTH.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR013748. Rep_factorC_C_dom.
    [Graphical view]
    PfamiPF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 3 hits.
    PF08542. Rep_fac_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 3 hits.
    SM00306. HintN. 3 hits.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF48019. SSF48019. 1 hit.
    SSF51294. SSF51294. 7 hits.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 3 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 3 hits.
    TIGR01445. intein_Nterm. 3 hits.
    PROSITEiPS50818. INTEIN_C_TER. 3 hits.
    PS50819. INTEIN_ENDONUCLEASE. 3 hits.
    PS50817. INTEIN_N_TER. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58817-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVIIMEKPWV EKYRPKTLDD IVGQDEIVKR LKKYVEKKSM PHLLFSGPPG     50
    VGKCLTGDTK VIVNGEIREI GEVIEEISNG KFGVTLTNNL KVLGIDEDGK 100
    IREFDVQYVY KDKTNTLIKI KTKMGRELKV TTYHPLLINH KNGEIKWEKA 150
    ENLKVGDKLA TPRYILFNES DYNEELAEWL GYFIGDGHAD KESNKITFTN 200
    GDEKLRKRFA ELTEKLFKDA KIKERIHKDR TPDIYVNSKE AVEFIDKLGL 250
    RGKKADKVRI PKEIMRSDAL RAFLRAYFDC DGGIEKHSIV LSTASKEMAE 300
    DLVYALLRFG IIAKLREKVN KNNNKVYYHI VISNSSNLRT FLDNIGFSQE 350
    RKLKKLLEII KDENPNLDVI TIDKEKIRYI RDRLKVKLTR DIEKDNWSYN 400
    KCRKITQELL KEIYYRLEEL KEIEKALEEN ILIDWDEVAE RRKEIAEKTG 450
    IRSDRILEYI RGKRKPSLKN YIKIANTLGK NIEKIIDAMR IFAKKYSSYA 500
    EIGKMLNMWN SSIKIYLESN TQEIEKLEEI RKTELKLVKE ILNDEKLIDS 550
    IGYVLFLASN EIYWDEIVEI EQLNGEFTIY DLHVPRYHNF IGGNLPTILH 600
    NTTAALCLAR DLFGENWRDN FLELNASVSK DTPILVKIDG KVKRTTFEEL 650
    DKIYFETNDE NEMYKKVDNL EVLTVDENFR VRWRKVSTII RHKVDKILRI 700
    KFEGGYIELT GNHSIMMLDE NGLVAKKASD IKVGDCFLSF VANIEGEKDR 750
    LDLKEFEPKD ITSRVKIIND FDIDEDTAWM LGLYVAEGAV GFKGKTSGQV 800
    IYTLGSHEHD LINKLNDIVD KKGFSKYENF TGSGFDRKRL SAKQIRILNT 850
    QLARFVEENF YDGNGRRARN KRIPDIIFEL KENLRVEFLK GLADGDSSGN 900
    WREVVRISSK SDNLLIDTVW LARISGIESS IFENEARLIW KGGMKWKKSN 950
    LLPAEPIIKM IKKLENKING NWRYILRHQL YEGKKRVSKD KIKQILEMVN 1000
    VEKLSDKEKE VYDLLKKLSK TELYALVVKE IEIIDYNDFV YDVSVPNNEM 1050
    FFAGNVPILL HNSDERGIDV IRTKVKDFAR TKPIGDVPFK IIFLDESDAL 1100
    TADAQNALRR TMEKYSDVCR FILSCLTGDA KITLPDEREI KIEDFIKMFE 1150
    ERKLKHVLNR NGEDLVLAGV KFNSKIVNHK VYRLVLESGR EIEATGDHKF 1200
    LTRDGWKEVY ELKEDDEVLV YPALEGVGFE VDERRIIGLN EFYEFLTNYE 1250
    IKLGYKPLGK AKSYKELITR DKEKILSRVL ELSDKYSKSE IRRKIEEEFG 1300
    IKISLTTIKN LINGKIDGFA LKYVRKIKEL GWDEITYDDE KAGIFARLLG 1350
    FIIGDGHLSK SKEGRILITA TINELEGIKK DLEKLGIKAS NIIEKDIEHK 1400
    LDGREIKGKT SFIYINNKAF YLLLNFWGVE IGNKTINGYN IPKWIKYGNK 1450
    FVKREFLRGL FGADGTKPYI KKYNINGIKL GIRVENISKD KTLEFFEEVK 1500
    KMLEEFEVES YIKVSKIDNK NLTELIVKAN NKNYLKYLSR ISYAYEKDNF 1550
    ARLVGEYLRI KEAYKDIILK EIAENALKEA DGEKSLRELA RKYNVPVDFI 1600
    INQLKGKDIG LPRNFMTFEE FLKEKVVDGK YVSERIIKKE CIGYRDVYDI 1650
    TCHKDPSFIA NGFVSHNCNY PSKIIPPIQS RCAVFRFSPL KKEDIAKKLK 1700
    EIAEKEGLNL TESGLEAIIY VSEGDMRKAI NVLQTAAALS DVIDDEIVYK 1750
    VSSRARPEEV KKMMELALDG KFMEARDLLY KLMVEWGMSG EDILNQMFRE 1800
    INSLDIDERK KVELADAIGE TDFRIVEGAN ERIQLSALLA KMALMGR 1847
    Length:1,847
    Mass (Da):214,097
    Last modified:November 1, 1996 - v1
    Checksum:i44EADB5B4D6DE64A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99433.1.
    PIRiE64477.
    RefSeqiNP_248426.1. NC_000909.1.
    WP_010870940.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99433; AAB99433; MJ_1422.
    GeneIDi1452326.
    KEGGimja:MJ_1422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99433.1 .
    PIRi E64477.
    RefSeqi NP_248426.1. NC_000909.1.
    WP_010870940.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58817.
    SMRi Q58817. Positions 2-53, 1058-1125, 1667-1847.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1422.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99433 ; AAB99433 ; MJ_1422 .
    GeneIDi 1452326.
    KEGGi mja:MJ_1422.

    Phylogenomic databases

    eggNOGi COG1372.
    KOi K04801.
    OMAi TNDENEM.

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    2.170.16.10. 7 hits.
    3.10.28.10. 5 hits.
    3.40.50.300. 4 hits.
    InterProi IPR001387. Cro/C1-type_HTH.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR013748. Rep_factorC_C_dom.
    [Graphical view ]
    Pfami PF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 3 hits.
    PF08542. Rep_fac_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 3 hits.
    SM00306. HintN. 3 hits.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF48019. SSF48019. 1 hit.
    SSF51294. SSF51294. 7 hits.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 3 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 3 hits.
    TIGR01445. intein_Nterm. 3 hits.
    PROSITEi PS50818. INTEIN_C_TER. 3 hits.
    PS50819. INTEIN_ENDONUCLEASE. 3 hits.
    PS50817. INTEIN_N_TER. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiRFCS_METJA
    AccessioniPrimary (citable) accession number: Q58817
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the potential ATP-binding site.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3