Q58816 (COBB_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cobyrinic acid A,C-diamide synthase

Gene names

Name:	cobB
Ordered Locus Names:	MJ1421

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. Contains 1 GATase cobBQ-type domain.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Domain	Glutamine amidotransferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Probable cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027		PRO_0000141276
Regions
	Domain	248 – 433	186	GATase cobBQ-type
Sites
	Active site	327	1	Nucleophile By similarity
	Active site	425	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58816 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EA8D66D0FC9ED212	FASTA	443	50,206
	10 20 30 40 50 60 MIMKRVVIAG TSSEVGKTVI STGIMKALSK KYNVQGYKVG PDYIDPTYHT IATGNKSRNL 70 80 90 100 110 120 DSFFMNKEQI KYLFQKHSKD KDISVIEGVR GLYEGISAID DIGSTASVAK ALDSPIILLV 130 140 150 160 170 180 NAKSLTRSAI AIIKGFMSFD NVKIKGVIFN FVRSENHIKK LKDAMSYYLP DIEIIGFIPR 190 200 210 220 230 240 NEDFKVEGRH LGLVPTPENL KEIESKIVLW GELVEKYLDL DKIVEIADED FEEVDDVFLW 250 260 270 280 290 300 EVNENYKKIA VAYDKAFNFY YWDNFEALKE NKAKIEFFSP LKDSEVPDAD ILYIGGGYPE 310 320 330 340 350 360 LFKEELSRNK EMIESIKEFD GYIYGECGGL MYITKSIDNV PMVGLLNCSA VMTKHVQGLS 370 380 390 400 410 420 YVKAEFLEDC LIGRKGLKFK GHEFHYSKLV NIKEERFAYK IERGRGIINN LDGIFNGKVL 430 440 AGYLHNHAVA NPYFASSMVN FGE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB99432.1.
PIR	D64477.
RefSeq	NP_248425.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58816.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ1421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB99432; AAB99432; MJ_1421.
GeneID	1452325.
KEGG	mja:MJ_1421.
Phylogenomic databases
eggNOG	COG1797.
KO	K02224.
OMA	CWLPGGY.
ProtClustDB	PRK01077.
Enzyme and pathway databases
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COBB_METJA

Accession

Primary (citable) accession number: Q58816

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program