Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]

Names and origin

Protein names

Recommended name:
    Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
    EC=2.6.1.16
Alternative name(s):
    Hexosephosphate aminotransferase
    D-fructose-6-phosphate amidotransferase
    GFAT
    L-glutamine-D-fructose-6-phosphate amidotransferase
    Glucosamine-6-phosphate synthase
Cleaved into the following chain:
    1- Recommended name:
            Mja gf6p intein

Gene names

Name:	glmS
Ordered Locus Names:	MJ1420

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

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Protein attributes

Sequence length	1099 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity.
Catalytic activity	L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP MF_00164
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	In the C-terminal section; belongs to the SIS family. GFAT subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 glutamine amidotransferase type-2 domain. Contains 1 HTH cro/C1-type DNA-binding domain. Contains 2 SIS domains.

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Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase Repeat
Molecular function	Aminotransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endonuclease activity Inferred from electronic annotation. Source: InterPro glutamine-fructose-6-phosphate transaminase (isomerizing) activity Inferred from electronic annotation. Source: HAMAP sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed By similarity

Chain

2 – 71

70

Glucosamine--fructose-6-phosphate aminotransferase [isomerizing], 1st part Potential

PRO_0000010848

Chain

72 – 570

499

Mja gf6p intein Potential

PRO_0000010849

Chain

571 – 1099

529

Glucosamine--fructose-6-phosphate aminotransferase [isomerizing], 2nd part Potential

PRO_0000010850

Regions

Domain

2 – 71

70

Glutamine amidotransferase type-2; first part

Domain

198 – 253

56

HTH cro/C1-type

Domain

278 – 413

136

DOD-type homing endonuclease

Domain

571 – 723

153

Glutamine amidotransferase type-2; second part

Domain

786 – 923

138

SIS 1

Domain

948 – 1089

142

SIS 2

Sites

Active site

2

1

Nucleophile; for GATase activity By similarity

Active site

1094

1

For Fru-6P isomerization activity By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q58815-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A4CFE30CB3894F16
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FASTA

1,099

125,501

        10         20         30         40         50         60 
MCGIIGYIGN DKAPKILLNG LRRLEYRGYD SCGIGVVDNN KLIIKKNVGK VEEVAKKERF 

        70         80         90        100        110        120 
LDIDGNIGIG HCLHPDTYVI LPDGRMKKIS EIDEDEVLSV NFEDLKLYNK KIKKFKHKAP 

       130        140        150        160        170        180 
KILYKIKTAF SELITTGEHK LFVVENGKIV EKCVKDLNGS ELIGVVRKLN YSFNDNVEFK 

       190        200        210        220        230        240 
DVYVERHYKL DETIRNKLRK VREKLGLTRK DVEKLCGVKE IYIVKIETGK LESIEEERLK 

       250        260        270        280        290        300 
KLCSLYGINF EEIIYRDNLH YTNPVKFPKT PTPELMQIIG YIIGDGHFPS NRMLRLKDER 

       310        320        330        340        350        360 
KEVLEEYNQL FKTVFNLEGN IKKGDGNYYI LEINSKYLID WFRENIPELF NKTGNERTPE 

       370        380        390        400        410        420 
FVFRLNNDLV ASYLRGIFDA EGYIRAEAKQ IGIGMTSKCF IKEIQFLLLR FGILASYSKI 

       430        440        450        460        470        480 
KRKEENWNNT HKLLISDKKS FELFKKYIGF TAKDKMEKLE AILNKMKGLN FRYISIPLTK 

       490        500        510        520        530        540 
KEIREFVGVP LKTIKNGDNY CTDYTIEKII EELNSKGLYD KAEYLKRFLD ADIVWTKFKI 

       550        560        570        580        590        600 
EEVESDVEYV YDLEVEDYHN FIGNLIINHN SRWATHGNVC KENAHPHTDC KEEIAVVHNG 

       610        620        630        640        650        660 
IISNYKELKD ELMKKGHKFK SETDTEVVPH LIEEELKKFK EINEENYIKA VKNAIKKLKG 

       670        680        690        700        710        720 
TYALVIINKN FPNLLIGARN ESPLILGIND DGYFLGSDIT AFLDYTNKAI PLEDGDVVVI 

       730        740        750        760        770        780 
KKKENGYEVT IENNGNTVER EMMEINWDIS SAEKMGYPHF MLKEIMEQPE VLKVSAKISA 

       790        800        810        820        830        840 
EEIKELAKCI KDYDRVYFVA MGTSLHAAMV VEYLFAKLGK LVIACDASEF LNKGVVDDKT 

       850        860        870        880        890        900 
LVIGITQSGE TYDTLKALRF AKKNKAKTGA IVNVLGSTAT READITVMMG AGIEIAVCAT 

       910        920        930        940        950        960 
KTYTSQLMIL YRLFIEYGKL LGRDMSEYEK EIDKIPNYIK EVLDKKETIK EIANNLKVNN 

       970        980        990       1000       1010       1020 
YIFISKGINI ASALEGALKF KEITYLHAEG MSGGLLKHGT ISLIDENMDT VAIVPPRDSA 

      1030       1040       1050       1060       1070       1080 
VFNSILSNIE EVKARGGKVI AITPTEIDGA ENILVPEVIE EISPIVYAPA FQLLAYYKAV 

      1090 
ELGRDVDKPR GLAKSVTVE

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References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii."
Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G.

Venter J.C.
Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Cross-references

Sequence databases
	L77117 Genomic DNA. Translation: AAB99430.1. Different initiation.
PIR	C64477.
RefSeq	NP_248424.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C44.971.
Genome annotation databases
GeneID	1452324.
GenomeReviews	Gene locus MJ1420 in contig L77117_GR.
KEGG	mja:MJ1420.
NMPDR	fig\|243232.1.peg.1461.
TIGR	MJ1420.
Enzyme and pathway databases
BRENDA	2.6.1.16. 256362.
Family and domain databases
HAMAP	MF_00164. Divergent sequence. [Tree]
InterPro	IPR000583. GATase_2. IPR017932. GATase_II. IPR005855. GlmS_trans. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR001387. HTH_3. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR010982. Lambda_DNA-bd. IPR001347. SIS. [Graphical view]
Pfam	PF00310. GATase_2. 2 hits. PF01381. HTH_3. 1 hit. PF01380. SIS. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00530. HTH_XRE. 1 hit. [Graphical view]
TIGRFAMs	TIGR01135. glmS. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS50943. HTH_CROC1. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS51464. SIS. 2 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

GLMS_METJA

Accession

Primary (citable) accession number: Q58815

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Intein-containing proteins

List of intein-containing protein entries

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents