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Q58815

- GLMS_METJA

UniProt

Q58815 - GLMS_METJA

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.By similarity

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Nucleophile; for GATase activityPROSITE-ProRule annotation
    Active sitei1094 – 10941For Fru-6P isomerization activityBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. endonuclease activity Source: InterPro
    3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-EC
    4. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. glutamine metabolic process Source: UniProtKB-KW
    3. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase
    GFAT
    Glucosamine-6-phosphate synthase
    Hexosephosphate aminotransferase
    L-glutamine--D-fructose-6-phosphate amidotransferase
    Cleaved into the following chain:
    Gene namesi
    Name:glmS
    Ordered Locus Names:MJ1420
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 7170Glutamine--fructose-6-phosphate aminotransferase [isomerizing], 1st partSequence AnalysisPRO_0000010848Add
    BLAST
    Chaini72 – 570499Mja gf6p inteinSequence AnalysisPRO_0000010849Add
    BLAST
    Chaini571 – 1099529Glutamine--fructose-6-phosphate aminotransferase [isomerizing], 2nd partSequence AnalysisPRO_0000010850Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi243232.MJ1420.

    Structurei

    3D structure databases

    ProteinModelPortaliQ58815.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 7170Glutamine amidotransferase type-2; first partPROSITE-ProRule annotationAdd
    BLAST
    Domaini198 – 25356HTH cro/C1-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini278 – 413136DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST
    Domaini571 – 723153Glutamine amidotransferase type-2; second partPROSITE-ProRule annotationAdd
    BLAST
    Domaini786 – 923138SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini948 – 1089142SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the SIS family. GFAT subfamily.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    KOiK00820.
    OMAiNSRWATH.

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    3.10.28.10. 2 hits.
    3.60.20.10. 2 hits.
    InterProiIPR001387. Cro/C1-type_HTH.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF13537. GATase_7. 1 hit.
    PF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    PF01380. SIS. 2 hits.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF51294. SSF51294. 3 hits.
    SSF55608. SSF55608. 1 hit.
    SSF56235. SSF56235. 2 hits.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q58815-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIIGYIGN DKAPKILLNG LRRLEYRGYD SCGIGVVDNN KLIIKKNVGK     50
    VEEVAKKERF LDIDGNIGIG HCLHPDTYVI LPDGRMKKIS EIDEDEVLSV 100
    NFEDLKLYNK KIKKFKHKAP KILYKIKTAF SELITTGEHK LFVVENGKIV 150
    EKCVKDLNGS ELIGVVRKLN YSFNDNVEFK DVYVERHYKL DETIRNKLRK 200
    VREKLGLTRK DVEKLCGVKE IYIVKIETGK LESIEEERLK KLCSLYGINF 250
    EEIIYRDNLH YTNPVKFPKT PTPELMQIIG YIIGDGHFPS NRMLRLKDER 300
    KEVLEEYNQL FKTVFNLEGN IKKGDGNYYI LEINSKYLID WFRENIPELF 350
    NKTGNERTPE FVFRLNNDLV ASYLRGIFDA EGYIRAEAKQ IGIGMTSKCF 400
    IKEIQFLLLR FGILASYSKI KRKEENWNNT HKLLISDKKS FELFKKYIGF 450
    TAKDKMEKLE AILNKMKGLN FRYISIPLTK KEIREFVGVP LKTIKNGDNY 500
    CTDYTIEKII EELNSKGLYD KAEYLKRFLD ADIVWTKFKI EEVESDVEYV 550
    YDLEVEDYHN FIGNLIINHN SRWATHGNVC KENAHPHTDC KEEIAVVHNG 600
    IISNYKELKD ELMKKGHKFK SETDTEVVPH LIEEELKKFK EINEENYIKA 650
    VKNAIKKLKG TYALVIINKN FPNLLIGARN ESPLILGIND DGYFLGSDIT 700
    AFLDYTNKAI PLEDGDVVVI KKKENGYEVT IENNGNTVER EMMEINWDIS 750
    SAEKMGYPHF MLKEIMEQPE VLKVSAKISA EEIKELAKCI KDYDRVYFVA 800
    MGTSLHAAMV VEYLFAKLGK LVIACDASEF LNKGVVDDKT LVIGITQSGE 850
    TYDTLKALRF AKKNKAKTGA IVNVLGSTAT READITVMMG AGIEIAVCAT 900
    KTYTSQLMIL YRLFIEYGKL LGRDMSEYEK EIDKIPNYIK EVLDKKETIK 950
    EIANNLKVNN YIFISKGINI ASALEGALKF KEITYLHAEG MSGGLLKHGT 1000
    ISLIDENMDT VAIVPPRDSA VFNSILSNIE EVKARGGKVI AITPTEIDGA 1050
    ENILVPEVIE EISPIVYAPA FQLLAYYKAV ELGRDVDKPR GLAKSVTVE 1099
    Length:1,099
    Mass (Da):125,501
    Last modified:January 23, 2007 - v3
    Checksum:iA4CFE30CB3894F16
    GO

    Sequence cautioni

    The sequence AAB99430.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99430.1. Different initiation.
    PIRiC64477.
    RefSeqiNP_248424.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB99430; AAB99430; MJ_1420.
    GeneIDi1452324.
    KEGGimja:MJ_1420.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB99430.1 . Different initiation.
    PIRi C64477.
    RefSeqi NP_248424.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q58815.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ1420.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB99430 ; AAB99430 ; MJ_1420 .
    GeneIDi 1452324.
    KEGGi mja:MJ_1420.

    Phylogenomic databases

    eggNOGi COG0449.
    KOi K00820.
    OMAi NSRWATH.

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    3.10.28.10. 2 hits.
    3.60.20.10. 2 hits.
    InterProi IPR001387. Cro/C1-type_HTH.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF13537. GATase_7. 1 hit.
    PF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    PF01380. SIS. 2 hits.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF51294. SSF51294. 3 hits.
    SSF55608. SSF55608. 1 hit.
    SSF56235. SSF56235. 2 hits.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiGLMS_METJA
    AccessioniPrimary (citable) accession number: Q58815
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3