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Q58815

- GLMS_METJA

UniProt

Q58815 - GLMS_METJA

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityPROSITE-ProRule annotation
Active sitei1094 – 10941For Fru-6P isomerization activityBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. endonuclease activity Source: InterPro
  3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-EC
  4. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-KW
  3. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Cleaved into the following chain:
Gene namesi
Name:glmS
Ordered Locus Names:MJ1420
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 7170Glutamine--fructose-6-phosphate aminotransferase [isomerizing], 1st partSequence AnalysisPRO_0000010848Add
BLAST
Chaini72 – 570499Mja gf6p inteinSequence AnalysisPRO_0000010849Add
BLAST
Chaini571 – 1099529Glutamine--fructose-6-phosphate aminotransferase [isomerizing], 2nd partSequence AnalysisPRO_0000010850Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243232.MJ1420.

Structurei

3D structure databases

ProteinModelPortaliQ58815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7170Glutamine amidotransferase type-2; first partPROSITE-ProRule annotationAdd
BLAST
Domaini198 – 25356HTH cro/C1-typePROSITE-ProRule annotationAdd
BLAST
Domaini278 – 413136DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST
Domaini571 – 723153Glutamine amidotransferase type-2; second partPROSITE-ProRule annotationAdd
BLAST
Domaini786 – 923138SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini948 – 1089142SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the SIS family. GFAT subfamily.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
InParanoidiQ58815.
KOiK00820.
OMAiNSRWATH.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
3.10.28.10. 2 hits.
3.60.20.10. 2 hits.
InterProiIPR001387. Cro/C1-type_HTH.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF13537. GATase_7. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF51294. SSF51294. 3 hits.
SSF55608. SSF55608. 1 hit.
SSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q58815-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIIGYIGN DKAPKILLNG LRRLEYRGYD SCGIGVVDNN KLIIKKNVGK
60 70 80 90 100
VEEVAKKERF LDIDGNIGIG HCLHPDTYVI LPDGRMKKIS EIDEDEVLSV
110 120 130 140 150
NFEDLKLYNK KIKKFKHKAP KILYKIKTAF SELITTGEHK LFVVENGKIV
160 170 180 190 200
EKCVKDLNGS ELIGVVRKLN YSFNDNVEFK DVYVERHYKL DETIRNKLRK
210 220 230 240 250
VREKLGLTRK DVEKLCGVKE IYIVKIETGK LESIEEERLK KLCSLYGINF
260 270 280 290 300
EEIIYRDNLH YTNPVKFPKT PTPELMQIIG YIIGDGHFPS NRMLRLKDER
310 320 330 340 350
KEVLEEYNQL FKTVFNLEGN IKKGDGNYYI LEINSKYLID WFRENIPELF
360 370 380 390 400
NKTGNERTPE FVFRLNNDLV ASYLRGIFDA EGYIRAEAKQ IGIGMTSKCF
410 420 430 440 450
IKEIQFLLLR FGILASYSKI KRKEENWNNT HKLLISDKKS FELFKKYIGF
460 470 480 490 500
TAKDKMEKLE AILNKMKGLN FRYISIPLTK KEIREFVGVP LKTIKNGDNY
510 520 530 540 550
CTDYTIEKII EELNSKGLYD KAEYLKRFLD ADIVWTKFKI EEVESDVEYV
560 570 580 590 600
YDLEVEDYHN FIGNLIINHN SRWATHGNVC KENAHPHTDC KEEIAVVHNG
610 620 630 640 650
IISNYKELKD ELMKKGHKFK SETDTEVVPH LIEEELKKFK EINEENYIKA
660 670 680 690 700
VKNAIKKLKG TYALVIINKN FPNLLIGARN ESPLILGIND DGYFLGSDIT
710 720 730 740 750
AFLDYTNKAI PLEDGDVVVI KKKENGYEVT IENNGNTVER EMMEINWDIS
760 770 780 790 800
SAEKMGYPHF MLKEIMEQPE VLKVSAKISA EEIKELAKCI KDYDRVYFVA
810 820 830 840 850
MGTSLHAAMV VEYLFAKLGK LVIACDASEF LNKGVVDDKT LVIGITQSGE
860 870 880 890 900
TYDTLKALRF AKKNKAKTGA IVNVLGSTAT READITVMMG AGIEIAVCAT
910 920 930 940 950
KTYTSQLMIL YRLFIEYGKL LGRDMSEYEK EIDKIPNYIK EVLDKKETIK
960 970 980 990 1000
EIANNLKVNN YIFISKGINI ASALEGALKF KEITYLHAEG MSGGLLKHGT
1010 1020 1030 1040 1050
ISLIDENMDT VAIVPPRDSA VFNSILSNIE EVKARGGKVI AITPTEIDGA
1060 1070 1080 1090
ENILVPEVIE EISPIVYAPA FQLLAYYKAV ELGRDVDKPR GLAKSVTVE
Length:1,099
Mass (Da):125,501
Last modified:January 23, 2007 - v3
Checksum:iA4CFE30CB3894F16
GO

Sequence cautioni

The sequence AAB99430.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99430.1. Different initiation.
PIRiC64477.
RefSeqiNP_248424.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB99430; AAB99430; MJ_1420.
GeneIDi1452324.
KEGGimja:MJ_1420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB99430.1 . Different initiation.
PIRi C64477.
RefSeqi NP_248424.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58815.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ1420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB99430 ; AAB99430 ; MJ_1420 .
GeneIDi 1452324.
KEGGi mja:MJ_1420.

Phylogenomic databases

eggNOGi COG0449.
InParanoidi Q58815.
KOi K00820.
OMAi NSRWATH.

Family and domain databases

Gene3Di 1.10.260.40. 1 hit.
3.10.28.10. 2 hits.
3.60.20.10. 2 hits.
InterProi IPR001387. Cro/C1-type_HTH.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF13537. GATase_7. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view ]
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF51294. SSF51294. 3 hits.
SSF55608. SSF55608. 1 hit.
SSF56235. SSF56235. 2 hits.
TIGRFAMsi TIGR01135. glmS. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiGLMS_METJA
AccessioniPrimary (citable) accession number: Q58815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3